Identification of piRNAs in the testes of Sunite and Small-tailed Han sheep.

Abstract:

:P-element-induced wimpy testis-interacting RNAs (piRNAs) are small RNAs that are essential for mammalian spermatogenesis and testicular development. Comparative analyses of the molecular mechanisms of spermatogenesis among different organisms are therefore dependent on accurate piRNA characterizations. In this study, we identified piRNAs in the testes of two breeds of Mongolian sheep: the Sunite (SN), which has a low reproductive rate, and Small-tailed Han (STH), which has a high reproductive rate. A thorough understanding of the mechanisms underlying the differences in fecundity between the two breeds might provide insights for the improvement of fertility and reproductive success in these and other sheep breeds. We identified 835 piRNAs and 206 piRNA clusters across the two breeds. Of these, 29 putative piRNAs were expressed in the SN samples only, and 229 putative piRNAs were expressed in the STH samples only. In addition, 206 piRNA clusters were upregulated in STH sheep as compared to the SN sheep. Functional pathway analysis indicated that the genes neighboring the predicted piRNAs were likely associated with spermatogenesis. piRNAs might thus be linked to male fecundity in sheep. Our results increase knowledge of the association between piRNAs and male fertility.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

He X,Li B,Fu S,Wang B,Qi Y,Da L,Te R,Sun S,Liu Y,Zhang W

doi

10.1080/10495398.2019.1640717

subject

Has Abstract

pub_date

2019-07-18 00:00:00

pages

1-8

eissn

1049-5398

issn

1532-2378

pub_type

杂志文章
  • Isolation of thirty-one new porcine microsatellites from a microsatellite enriched microdissected chromosome 8 library.

    abstract::Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390009525945

    authors: Wang Z,Rohrer G,Stone R,Troyer D

    更新日期:2000-01-01 00:00:00

  • Mutations in Leptin (LEP) Gene Are Associated with Carcass and Meat Quality Traits in Crossbreed Rabbits.

    abstract::Leptin is a hormone synthesized and secreted primarily in adipose cells that help to regulate energy balance. This study examined the associations of single nucleotide polymorphisms in the rabbit leptin gene with growth traits, slaughter traits and physicochemical parameters of New Zealand White (NZW) and Belgian Gian...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1347881

    authors: Migdal L,Koziol K,Palka S,Migdal W,Zabek T,Otwinowska-Mindur A,Migdal A,Kmiecik M,Maj D,Bieniek J

    更新日期:2018-04-03 00:00:00

  • Differential Transcriptome Analysis of Early Postnatal Developing Longissimus Dorsi Muscle from Two Pig Breeds Characterized in Divergent Myofiber Traits and Fatness.

    abstract::Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal m...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2018.1437045

    authors: Xu X,Mishra B,Qin N,Sun X,Zhang S,Yang J,Xu R

    更新日期:2019-01-01 00:00:00

  • Association of genetic variability in CD209 gene with bovine paratuberculosis disease: a case-control study in the Indian cattle population.

    abstract::This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1823400

    authors: Kumar S,Kumar S,Singh RV,Chauhan A,Kumar A,Bharati J,Singh SV

    更新日期:2020-09-28 00:00:00

  • Comparative pharmacokinetic study of two kinds of altrenogest oral solutions for sows.

    abstract::This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1723608

    authors: Li Y,Yang H,Xia L,Wang S,Bu S

    更新日期:2020-03-17 00:00:00

  • Analysis of association between the MUC4 g.8227C>G polymorphism and production traits in Italian heavy pigs using a selective genotyping approach.

    abstract::In pigs, susceptibility to enterotoxigenic Escherichia coli (ETEC) K88 strains (locus F4bcR) is determined by a dominant allele, with the recessive allele determining resistance. The susceptible allele also appeared to be associated with higher growth rate even with discordant results. A single nucleotide polymorphism...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2011.653462

    authors: Fontanesi L,Bertolini F,Dall'Olio S,Buttazzoni L,Gallo M,Russo V

    更新日期:2012-01-01 00:00:00

  • Candidate gene approach: potentional association of caspase-1, inhibitor of apoptosis protein-1, and prosaposin gene polymorphisms with response to Salmonella enteritidis challenge or vaccination in young chicks.

    abstract::Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-120022136

    authors: Liu W,Lamont SJ

    更新日期:2003-05-01 00:00:00

  • Analysis of allele segregation distortion in a swine resource family.

    abstract::Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495399909525939

    authors: Yasue H,Mikawa S,Uenishi H,Wada Y

    更新日期:1999-01-01 00:00:00

  • Genomic organization and promoter analysis of the bovine ADAM12 gene.

    abstract::A disintegrin and metalloprotease (ADAM) 12 is a member of the ADAM family possessing a putative role in a variety of biological processes such as modulation of proteolytic processing, cell adhesion, cell fusion, and signaling. Recently, it has been suggested that ADAM12 is involved in regulation of adipogenesis as we...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390802212445

    authors: Taniguchi Y,Doronbekov K,Yamada T,Sasaki Y,Takano A,Sugimoto Y

    更新日期:2008-01-01 00:00:00

  • Expressional and Bioinformatic Analysis of Bovine Filia/Ecat1/Khdc3l Gene: A Comparison with Ovine Species.

    abstract::Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, li...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2016.1157081

    authors: Zahmatkesh A,Ansari Mahyari S,Daliri Joupari M,Rahmani H,Shirazi A,Amiri Roudbar M,Ansari Majd S

    更新日期:2016-01-01 00:00:00

  • Effects of Recombinant Human Lactoferrin on Osteoblast Growth and Bone Status in Piglets.

    abstract::Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1313269

    authors: Li Q,Zhao J,Hu W,Wang J,Yu T,Dai Y,Li N

    更新日期:2018-04-03 00:00:00

  • Lipopolysaccharide binding protein/CD14/ TLR4-dependent recognition of salmonella LPS induces the functional activation of chicken heterophils and up-regulation of pro-inflammatory cytokine and chemokine gene expression in these cells.

    abstract::Lipopolysaccharide (LPS) is the major pathogen-associated molecular pattern (PAMP) found in the cell wall of gram-negative bacteria and, in mammals, is recognized by the Toll-like receptor 4 (TLR4) in conjunction with the serum protein, lipopolysaccharide-binding protein (LBP), and the CD14 co-receptor. We have found ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390500264896

    authors: Kogut MH,He H,Kaiser P

    更新日期:2005-01-01 00:00:00

  • Production of single-chain fragment variable (scFv) antibodies specific to plasma membrane epitopes on bull Y-bearing sperm.

    abstract::Distinguishing between bull Y- and X-bearing sperm populations is advantageous for techniques using sexed bull semen. The aim of this study was to produce a single-chain fragment variable (scFv) antibody against plasma membrane epitopes on bull Y-bearing sperm. Variable heavy (VH)- and variable light (VL)-region genes...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1811294

    authors: Thaworn W,Hongsibsong S,Thongkham M,Mekchay S,Pattanawong W,Sringarm K

    更新日期:2020-08-31 00:00:00

  • Association between polymorphisms in the SLC27A1 gene and milk production traits in Chinese Holstein cattle.

    abstract::We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2011.527567

    authors: Lv Y,Wei C,Zhang L,Lu G,Liu K,Du L

    更新日期:2011-01-01 00:00:00

  • Candidate SNP of CACNA2D1 Gene Associated with Clinical Mastitis and Production Traits in Sahiwal (Bos taurus indicus) and Karan Fries (Bos taurus taurus × Bos taurus indicus).

    abstract::The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2018.1437046

    authors: Magotra A,Gupta ID,Verma A,Alex R,Mr V,Ahmad T

    更新日期:2019-01-01 00:00:00

  • Establishment and characterization of proliferating primary cultures of equine epidermal keratinocytes.

    abstract::Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2019.1687091

    authors: Ogorevc J,Poklukar K,Dovč P

    更新日期:2019-11-18 00:00:00

  • Partial cloning and expression of the bovine leptin gene.

    abstract::The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495399809525887

    authors: Ji S,Willis GM,Scott RR,Spurlock ME

    更新日期:1998-01-01 00:00:00

  • Interferon-alpha genes from Bos and Bubalus bubalus.

    abstract::Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390500461104

    authors: Shi X,Xia C,Pan B,Wang M

    更新日期:2006-01-01 00:00:00

  • The specificity protein 3 (SP3) gene in ducks (Anas platyrhynchos): cloning, characterization and expression during viral infection.

    abstract::Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck SP3 (duSP3). The full-length cDNA sequence of the duSP3 gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, includ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1740240

    authors: Cao Z,Gao W,Gu T,Huo W,Zhang Y,Zhang Y,Xu Q,Chen G

    更新日期:2020-03-17 00:00:00

  • Cloning of bovine pyruvate carboxylase and 5' untranslated region variants.

    abstract::Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-120037897

    authors: Agca C,Bidwell CA,Donkin SS

    更新日期:2004-05-01 00:00:00

  • A novel 17 bp InDel polymorphism within the PPARGC1A gene is significantly associated with growth traits in sheep.

    abstract::Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a member of transcriptional coactivator of the peroxisome proliferator-activated receptor. It is involved in lipid metabolism, energy metabolism, adipocyte differentiation and regulation of mitochondrial biogenesis. Therefore, the genet...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1796697

    authors: Chen P,Zhao H,Wu M,He S,Yuan T,Yi X,Liu S,Pan Y,Li Q,Wang S,Sun X

    更新日期:2020-08-09 00:00:00

  • Comparison of Copy Number of HSF Genes in Two Buffalo Genomes.

    abstract::The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations foll...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2015.1135807

    authors: Lal SV,Mukherjee A,Brahma B,Gohain M,Patra MC,Saini SK,Mishra P,Ahlawat S,Upadhyaya RC,Datta TK,De S

    更新日期:2016-01-01 00:00:00

  • Genetic variation at the MHC in a population of introduced wild turkeys.

    abstract::Genetic variation in the major histocompatibility complex (MHC) is known to affect disease resistance in many species. Investigations of MHC diversity in populations of wild species have focused on the antigen presenting class IIβ molecules due to the known polymorphic nature of these genes and the role these molecule...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2013.767267

    authors: Bauer MM,Miller MM,Briles WE,Reed KM

    更新日期:2013-01-01 00:00:00

  • Construction of a CRISPR-Cas9 System for Pig Genome Targeting.

    abstract::A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2015.1027774

    authors: Su YH,Lin TY,Huang CL,Tu CF,Chuang CK

    更新日期:2015-01-01 00:00:00

  • Genetic structuring of nine Indian domestic goat breeds based on SNPs identified in IGF-1 gene.

    abstract::The caprine Insulin like Growth Factor1 (IGF1) gene was analyzed for identification of single nucleotide polymorphisms (SNPs) and genetic structuring of Indian goat breeds. A panel of 80 samples belonging to nine Indian goat breeds (Capra hircus) including three large sized breeds (Jamunapari, Beetal and Jakhrana); th...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2013.763815

    authors: Sharma A,Dutt G,Jayakumar S,Saroha V,Verma NK,Dixit SP

    更新日期:2013-01-01 00:00:00

  • Combined Haplotypes of CaSR Gene Sequence Variants and Their Associations with Growth Traits in Cattle.

    abstract::The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2016.1271805

    authors: Yue B,Han F,Wu J,Wang Y,Zhang C,Fang X,Qi X,Bai Y,Chen H

    更新日期:2017-10-02 00:00:00

  • Development and Utilization of VHH Antibodies Derived from Camelus Dromedarius Against Foot-and-Mouth Disease Virus.

    abstract::Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2018.1433191

    authors: Dash L,Subramaniam S,Khulape SA,Prusty BR,Pargai K,Narnaware SD,Patil NV,Pattnaik B

    更新日期:2019-01-01 00:00:00

  • Use of touch-down polymerase chain reaction to enhance the sensitivity of Brucella melitensis detection in raw milk.

    abstract::Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1777149

    authors: Rahimi H,Tukmechi A,Rashidian E

    更新日期:2020-06-10 00:00:00

  • Association of the porcine transforming growth factor beta type I receptor (TGFBR1) gene with growth and carcass traits.

    abstract:BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2011.630897

    authors: Chen K,Hawken R,Flickinger GH,Rodriguez-Zas SL,Rund LA,Wheeler MB,Abrahamsen M,Rutherford MS,Beever JE,Schook LB

    更新日期:2012-01-01 00:00:00

  • Simple sequence repeats for genetic studies of alpaca.

    abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390802340923

    authors: Reed KM,Chaves LD

    更新日期:2008-01-01 00:00:00