Use of touch-down polymerase chain reaction to enhance the sensitivity of Brucella melitensis detection in raw milk.

abstract：:In the present study, expression level of various ATP-binding cassette (ABC) viz., ABCA1, ABCA7, ABCG1, ABCG2, and ABCG5; associated transcription factors viz., SREBF1, LXRα (NR1H3), PPARA, and Solute Carriers (SLC); or Glucose transporters (GLUT) viz., SLC2A1(GLUT1), SLC2A4 (GLUT4), SLC2A8 (GLUT8), and SLC2A12 (GLUT1...

journal_title：Animal biotechnology

authors： Sharma A,Aggarwal J,Sodhi M,Kishore A,Mishra BP,Mohanty AK,Kataria RS,Kaushik JK,Mukesh M

更新日期：2014-01-01 00:00:00

abstract：:The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations foll...

journal_title：Animal biotechnology

authors： Lal SV,Mukherjee A,Brahma B,Gohain M,Patra MC,Saini SK,Mishra P,Ahlawat S,Upadhyaya RC,Datta TK,De S

更新日期：2016-01-01 00:00:00

abstract：:Numerous studies have demonstrated that FTO plays an important role in adipogenesis. Herein, we designed a small interfering RNA targeting FTO to knock down its endogenous expression and investigated its effects on the proliferation and differentiation of porcine intramuscular preadipocytes. Its possible mechanism was...

journal_title：Animal biotechnology

authors： Chen X,Luo Y,Jia G,Liu G,Zhao H,Huang Z

更新日期：2017-10-02 00:00:00

abstract：:The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...

journal_title：Animal biotechnology

authors： Huang L,Lin Y,Jin S,Liu W,Xu Y,Zheng Y

更新日期：2012-04-01 00:00:00

abstract：:The Jining Grey is a prolific local goat breed in P.R. China. Bone morphogenetic protein 15 (BMP15) gene that controls high fecundity of Inverdale, Hanna, Lacaune, Belclare, Cambridge, and Small Tailed Han ewes was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of ovine...

journal_title：Animal biotechnology

authors： Chu MX,Jiao CL,He YQ,Wang JY,Liu ZH,Chen GH

更新日期：2007-01-01 00:00:00

abstract：:Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...

journal_title：Animal biotechnology

authors： Wang Z,Rohrer G,Stone R,Troyer D

更新日期：2000-01-01 00:00:00

abstract：:Ovarian transvaginal ultrasonography (OTU) has been used world-wide for commercial ovum pick-up programs for in vitro embryo production in elite herds, providing an excellent model for the elucidation of factors controlling bovine oocyte developmental competence. Noninvasive sampling and treatment of ovarian structure...

journal_title：Animal biotechnology

authors： Velazquez MA,Kues WA,Niemann H

更新日期：2014-01-01 00:00:00

abstract：:P-element-induced wimpy testis-interacting RNAs (piRNAs) are small RNAs that are essential for mammalian spermatogenesis and testicular development. Comparative analyses of the molecular mechanisms of spermatogenesis among different organisms are therefore dependent on accurate piRNA characterizations. In this study, ...

journal_title：Animal biotechnology

authors： He X,Li B,Fu S,Wang B,Qi Y,Da L,Te R,Sun S,Liu Y,Zhang W

更新日期：2019-07-18 00:00:00

abstract：:Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...

journal_title：Animal biotechnology

authors： Liu W,Lamont SJ

更新日期：2003-05-01 00:00:00

abstract：:Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...

journal_title：Animal biotechnology

authors： Shi X,Xia C,Pan B,Wang M

更新日期：2006-01-01 00:00:00

abstract：:Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...

journal_title：Animal biotechnology

authors： Yasue H,Mikawa S,Uenishi H,Wada Y

更新日期：1999-01-01 00:00:00

abstract：:The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar...

journal_title：Animal biotechnology

authors： Mohalik PK,Sahoo SS,Mishra C,Dash SK,Nayak G

更新日期：2020-02-12 00:00:00

abstract：:A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H a...

journal_title：Animal biotechnology

authors： Prinzenberg EM,Krause I,Erhardt G

更新日期：1999-01-01 00:00:00

abstract：:The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...

journal_title：Animal biotechnology

authors： Rogatcheva MB,Rund LA,Beever JE,Schook LB

更新日期：2003-11-01 00:00:00

abstract：:A disintegrin and metalloprotease (ADAM) 12 is a member of the ADAM family possessing a putative role in a variety of biological processes such as modulation of proteolytic processing, cell adhesion, cell fusion, and signaling. Recently, it has been suggested that ADAM12 is involved in regulation of adipogenesis as we...

journal_title：Animal biotechnology

authors： Taniguchi Y,Doronbekov K,Yamada T,Sasaki Y,Takano A,Sugimoto Y

更新日期：2008-01-01 00:00:00

abstract：:In pigs, susceptibility to enterotoxigenic Escherichia coli (ETEC) K88 strains (locus F4bcR) is determined by a dominant allele, with the recessive allele determining resistance. The susceptible allele also appeared to be associated with higher growth rate even with discordant results. A single nucleotide polymorphism...

journal_title：Animal biotechnology

authors： Fontanesi L,Bertolini F,Dall'Olio S,Buttazzoni L,Gallo M,Russo V

更新日期：2012-01-01 00:00:00

abstract：:The aim of the study was to introduce a convenient method for identification of differences among individual animals in genes supposed to influence meat performance in pigs. The set of seven candidate genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF, and MC3R) was used. To determine the genotypes, multiplex polymerase chain r...

journal_title：Animal biotechnology

authors： Civánová K,Knoll A

更新日期：2007-01-01 00:00:00

abstract：:The present study was undertaken to characterize the structure of S100A8 gene and its promoter in water buffalo and yak. Sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete S100A8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. BLAST analys...

journal_title：Animal biotechnology

authors： Kathiravan P,Goyal S,Kataria RS,Mishra BP,Jayakumar S,Joshi BK

更新日期：2011-07-01 00:00:00

abstract：:The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...

journal_title：Animal biotechnology

authors： Yue B,Han F,Wu J,Wang Y,Zhang C,Fang X,Qi X,Bai Y,Chen H

更新日期：2017-10-02 00:00:00

abstract：:High concentrate (HC) diet feeding leads to the lysis of rumen microbes and the release of hazardous metabolites, which can trigger inflammatory responses, thereby impairing dairy cow health and production. γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP), which constitutes the peptidoglycan (PGN) layer of bacteria, is ...

journal_title：Animal biotechnology

authors： Wang Y,Gao Q,Cheng X,Chang G,Roy AC,Shen X

更新日期：2021-01-24 00:00:00

abstract：:Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...

journal_title：Animal biotechnology

authors： Boudali SF,Al-Jumaili AS,Bouandas A,Mahammi FZ,Tabet Aoul N,Hanotte O,Gaouar SBS

更新日期：2020-08-13 00:00:00

abstract：:This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Surviv...

journal_title：Animal biotechnology

authors： Wei X,Sijie Y,Weibin Z,Qing X,Jie Z,Xiangdong Z

更新日期：2018-01-01 00:00:00

abstract：:Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...

journal_title：Animal biotechnology

authors： Dash L,Subramaniam S,Khulape SA,Prusty BR,Pargai K,Narnaware SD,Patil NV,Pattnaik B

更新日期：2019-01-01 00:00:00

abstract：:The first hypervariable (HV1) region of mitochondrial DNA (mtDNA) of two popular Nigerian goat breeds: West African Dwarf (WAD) (n=35) and Red Sokoto (RS) (n=37) and one exotic breed: Kalahari Red (KR) (n=38) imported from South Africa were sequenced to investigate sequence diversity, genetic structure, origin, and de...

journal_title：Animal biotechnology

authors： Awotunde EO,Bemji MN,Olowofeso O,James IJ,Ajayi OO,Adebambo AO

更新日期：2015-01-01 00:00:00

abstract：:In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their perform...

journal_title：Animal biotechnology

authors： Dash S,Singh A,Bhatia AK,Jayakumar S,Sharma A,Singh S,Ganguly I,Dixit SP

更新日期：2018-04-03 00:00:00

abstract：:Calpains are Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed micro- and m-calpains. Both are heterodimers, consisting of a distinct catalytic subunit and a common regulatory subunit. We describe cloning and sequencing of the calpain small (regulatory) subunit (cpns) cDNA from rain...

journal_title：Animal biotechnology

authors： Salem M,Nath J,Killefer J

更新日期：2004-01-01 00:00:00

abstract：:Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck SP3 (duSP3). The full-length cDNA sequence of the duSP3 gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, includ...

journal_title：Animal biotechnology

authors： Cao Z,Gao W,Gu T,Huo W,Zhang Y,Zhang Y,Xu Q,Chen G

更新日期：2020-03-17 00:00:00

abstract：:The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of Brucella abortus strain 544 (S544) in the spleen of mice for potency testing of live B. abortus strain 19 (S19) vaccine. IS711 and eryC gene-based qPCR were optimized for calculating copy number. The copy ...

journal_title：Animal biotechnology

authors： Gupta T,Rawat M,Kumar B,Varshney R,Chakravarti S,Qureshi S

更新日期：2020-04-02 00:00:00

abstract：BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...

journal_title：Animal biotechnology

authors： Chen K,Hawken R,Flickinger GH,Rodriguez-Zas SL,Rund LA,Wheeler MB,Abrahamsen M,Rutherford MS,Beever JE,Schook LB

更新日期：2012-01-01 00:00:00

abstract：:Aldoketoreductase 1B5 (AKR1B5), a member of the Aldoketoreductase family, is involved in the production of Prostaglandin F2α (PGF2α) as one of vital prostaglandin F synthase (PGFS). PGs (Prostaglandins) play a crucial role in female reproductive system. In the present study, we cloned and characterized the full-length...

journal_title：Animal biotechnology

authors： Kumar R,Ramteke PW,Sharma SK,Mitra A

更新日期：2015-01-01 00:00:00