Cloning of bovine pyruvate carboxylase and 5' untranslated region variants.

Abstract:

:Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'UTR variants ranging from 68 to 363 bp were cloned. Bovine PC 5'UTR (bPC5') variants contain 68 (bPC5'A), 263 (bPC5'B), 363 (bPC5'C), 89 (bPC5'D), 275 (bPC5'E), and 178 bp (bPC5'F). All variants contain a common coding sequence. An RNase protection assay and RT-PCR analysis confirms the presence of the 5'UTR variants. The abundance of PC mRNA, determined by Northern blot analysis, indicates that PC is more abundant in gluconeogenic and lipogenic tissues where all PC variants are expressed compared with tissues that do not possess the full spectrum of PC transcripts. The data suggest that bPC5'A, bPC5'B, and bPC5'F are more abundant in bovine liver than the other variants.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Agca C,Bidwell CA,Donkin SS

doi

10.1081/ABIO-120037897

keywords:

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

47-66

issue

1

eissn

1049-5398

issn

1532-2378

journal_volume

15

pub_type

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