Construction of a CRISPR-Cas9 System for Pig Genome Targeting.

abstract：BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...

journal_title：Animal biotechnology

authors： Chen K,Hawken R,Flickinger GH,Rodriguez-Zas SL,Rund LA,Wheeler MB,Abrahamsen M,Rutherford MS,Beever JE,Schook LB

更新日期：2012-01-01 00:00:00

abstract：:Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...

journal_title：Animal biotechnology

authors： Dash L,Subramaniam S,Khulape SA,Prusty BR,Pargai K,Narnaware SD,Patil NV,Pattnaik B

更新日期：2019-01-01 00:00:00

abstract：:Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...

journal_title：Animal biotechnology

authors： Li Q,Zhao J,Hu W,Wang J,Yu T,Dai Y,Li N

更新日期：2018-04-03 00:00:00

abstract：:Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...

journal_title：Animal biotechnology

authors： Wang Z,Rohrer G,Stone R,Troyer D

更新日期：2000-01-01 00:00:00

abstract：:Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...

journal_title：Animal biotechnology

authors： Rahimi H,Tukmechi A,Rashidian E

更新日期：2020-06-10 00:00:00

abstract：:Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this stu...

journal_title：Animal biotechnology

authors： Zhong Z,Zhu X,Tang Q,Hong L,Gu Y,He Z,Tao X,Yang X,Liang Y,Shen L,Tan Y,Zeng K,Ying S,Yang Y,Lei Y,Wang Y,Gong J,Chen X,Zhou R,Zhu L,Lv X

更新日期：2021-01-18 00:00:00

abstract：:The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...

journal_title：Animal biotechnology

authors： Magotra A,Gupta ID,Verma A,Alex R,Mr V,Ahmad T

更新日期：2019-01-01 00:00:00

abstract：:For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution (0.5 M Sucrose) an...

journal_title：Animal biotechnology

authors： Yang ZQ,Zhou GB,Hou YP,Yan CL,Zhu SE

更新日期：2007-01-01 00:00:00

abstract：:To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...

journal_title：Animal biotechnology

authors： Burk NE,Messer LA,Ernst CW,Rothschild MF

更新日期：1998-01-01 00:00:00

abstract：:In the present study, expression level of various ATP-binding cassette (ABC) viz., ABCA1, ABCA7, ABCG1, ABCG2, and ABCG5; associated transcription factors viz., SREBF1, LXRα (NR1H3), PPARA, and Solute Carriers (SLC); or Glucose transporters (GLUT) viz., SLC2A1(GLUT1), SLC2A4 (GLUT4), SLC2A8 (GLUT8), and SLC2A12 (GLUT1...

journal_title：Animal biotechnology

authors： Sharma A,Aggarwal J,Sodhi M,Kishore A,Mishra BP,Mohanty AK,Kataria RS,Kaushik JK,Mukesh M

更新日期：2014-01-01 00:00:00

abstract：:Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP...

journal_title：Animal biotechnology

authors： Yonekura S,Hirota S,Miyazaki H,Tokutake Y

更新日期：2016-01-01 00:00:00

abstract：:The present study was undertaken to characterize the structure of S100A8 gene and its promoter in water buffalo and yak. Sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete S100A8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. BLAST analys...

journal_title：Animal biotechnology

authors： Kathiravan P,Goyal S,Kataria RS,Mishra BP,Jayakumar S,Joshi BK

更新日期：2011-07-01 00:00:00

abstract：:Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...

journal_title：Animal biotechnology

authors： Ogorevc J,Poklukar K,Dovč P

更新日期：2019-11-18 00:00:00

abstract：:We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...

journal_title：Animal biotechnology

authors： Lv Y,Wei C,Zhang L,Lu G,Liu K,Du L

更新日期：2011-01-01 00:00:00

abstract：:Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, li...

journal_title：Animal biotechnology

authors： Zahmatkesh A,Ansari Mahyari S,Daliri Joupari M,Rahmani H,Shirazi A,Amiri Roudbar M,Ansari Majd S

更新日期：2016-01-01 00:00:00

abstract：:A disintegrin and metalloprotease (ADAM) 12 is a member of the ADAM family possessing a putative role in a variety of biological processes such as modulation of proteolytic processing, cell adhesion, cell fusion, and signaling. Recently, it has been suggested that ADAM12 is involved in regulation of adipogenesis as we...

journal_title：Animal biotechnology

authors： Taniguchi Y,Doronbekov K,Yamada T,Sasaki Y,Takano A,Sugimoto Y

更新日期：2008-01-01 00:00:00

abstract：:Skeletal myoblast differentiation is controlled by a multitude of transcription factors and signal pathways. Myogenin is a critical transcriptional regulator in the initiation and maintenance of myoblast differentiation. The Akt/FoxO1 signal pathway plays an important role in myoblast differentiation. MicroRNAs are a ...

journal_title：Animal biotechnology

authors： Zhang S,Chen X,Huang Z,Chen D,Yu B,He J,Zheng P,Yu J,Luo J,Luo Y,Chen H

更新日期：2018-07-03 00:00:00

abstract：:Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY...

journal_title：Animal biotechnology

authors： Soleymani B,Hafezian SH,Mianji GR,Mansouri K,Chaharaein B,Tajehmiri A,Sharifi Tabar M,Mostafaie A

更新日期：2017-01-02 00:00:00

abstract：:Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...

journal_title：Animal biotechnology

authors： Agca C,Bidwell CA,Donkin SS

更新日期：2004-05-01 00:00:00

abstract：:Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal m...

journal_title：Animal biotechnology

authors： Xu X,Mishra B,Qin N,Sun X,Zhang S,Yang J,Xu R

更新日期：2019-01-01 00:00:00

abstract：:MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered in model animals. To explore the function and mechanism of miR-1 in goat, we firstly explored the expression profile of miR-1 in goat tissues and cells. Furthermore, the target gene of miR-1 ...

journal_title：Animal biotechnology

authors： Sui M,Zheng Q,Wu H,Zhu L,Ling Y,Wang L,Fang F,Liu Y,Zhang Z,Chu M,Zhang Y

更新日期：2020-10-01 00:00:00

abstract：:The current study designed to evaluate the effect of oligosaccharide supplemented diets on growth performance, histomorphometric changes, economic efficiency and genetic expression of some growth and immunity-relative genes. One hundred and twenty weaned male rabbits, six weeks of age of two breeds (NZW and APPRI) wer...

journal_title：Animal biotechnology

authors： Abd El-Aziz AH,El-Kasrawy NI,Abd El-Hack ME,Kamel SZ,Mahrous UE,El-Deeb EM,Atta MS,Amer MS,Naiel MAE,Khafaga AF,Metwally AE,Abo Ghanima MM

更新日期：2020-07-31 00:00:00

abstract：:The α-adducin (ADD1) is a subunit of adducin which is a cytoskeleton heterodimeric protein. Adducin participates in oocytes chromosome meiosis of mice, prompting adducin has an effect on embryonic development. Adducin gene mutation has significantly functional change. So the present study was to identify and character...

journal_title：Animal biotechnology

authors： Huang YZ,Qian LN,Wang J,Zhang CL,Fang XT,Lei CZ,Lan XY,Ma Y,Bai YY,Lin FP,Chen H

更新日期：2019-01-01 00:00:00

abstract：:Genetic variation in the major histocompatibility complex (MHC) is known to affect disease resistance in many species. Investigations of MHC diversity in populations of wild species have focused on the antigen presenting class IIβ molecules due to the known polymorphic nature of these genes and the role these molecule...

journal_title：Animal biotechnology

authors： Bauer MM,Miller MM,Briles WE,Reed KM

更新日期：2013-01-01 00:00:00

abstract：:This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...

journal_title：Animal biotechnology

authors： Kumar S,Kumar S,Singh RV,Chauhan A,Kumar A,Bharati J,Singh SV

更新日期：2020-09-28 00:00:00

abstract：:The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...

journal_title：Animal biotechnology

authors： Tantia MS,Vijh RK,Mishra B,Kumar ST

更新日期：2007-01-01 00:00:00

abstract：:Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternati...

journal_title：Animal biotechnology

authors： Nygard AB,Jørgensen CB,Cirera S,Fredholm M

更新日期：2010-10-01 00:00:00

abstract：:The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...

journal_title：Animal biotechnology

authors： Fries R,Ewald D,Thaller G,Buitkamp J

更新日期：2001-05-01 00:00:00

abstract：:The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...

journal_title：Animal biotechnology

authors： Yue B,Han F,Wu J,Wang Y,Zhang C,Fang X,Qi X,Bai Y,Chen H

更新日期：2017-10-02 00:00:00

abstract：:Since World War II the use of antimicrobial products associated with food animal production has increased. Antimicrobials along with evolving production practices have significantly increased throughput, animal welfare, and improved health. Concerns surrounding the growing significance of emerging and in some cases ra...

journal_title：Animal biotechnology

authors： Baker R

更新日期：2006-01-01 00:00:00