Abstract:
:Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck SP3 (duSP3). The full-length cDNA sequence of the duSP3 gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, including a 111 base pair (bp) 5'-untranslated region (UTR), 215 bp 3'-UTR, and 2142 bp open reading frame (ORF), which encoded a 713 amino acid (AA) strongly conserved with Avian SP3. Tissue specificity analysis demonstrated that duSP3 was constitutively expressed in the eight tissues tested: liver, spleen, lung, heart, kidney, thymus, breast, and leg; and low expression levels were observed in all tissues, except the spleen and thymus. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that duSP3 expression rapidly increased in vitro after stimulation with both the hepatitis virus (DHV-1) and polyriboinosinic polyribocytidylic acid (poly(I:C)). However, the expression under these treatments varied in kidney and liver tissues; in the liver, duSP3 increased significantly at 36 h after the DHV-1 treatment and peaked at 72 h after poly(I:C) stimulation. These results suggested that SP3 may play a positive role in immune responses against viral infections in ducks.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Cao Z,Gao W,Gu T,Huo W,Zhang Y,Zhang Y,Xu Q,Chen Gdoi
10.1080/10495398.2020.1740240subject
Has Abstractpub_date
2020-03-17 00:00:00pages
1-7eissn
1049-5398issn
1532-2378pub_type
杂志文章abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/abio-100102977
更新日期:2001-05-01 00:00:00
abstract::The α-adducin (ADD1) is a subunit of adducin which is a cytoskeleton heterodimeric protein. Adducin participates in oocytes chromosome meiosis of mice, prompting adducin has an effect on embryonic development. Adducin gene mutation has significantly functional change. So the present study was to identify and character...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1398754
更新日期:2019-01-01 00:00:00
abstract::Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1102148
更新日期:2016-01-01 00:00:00
abstract::Leptin is a hormone synthesized and secreted primarily in adipose cells that help to regulate energy balance. This study examined the associations of single nucleotide polymorphisms in the rabbit leptin gene with growth traits, slaughter traits and physicochemical parameters of New Zealand White (NZW) and Belgian Gian...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1347881
更新日期:2018-04-03 00:00:00
abstract::The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.652325
更新日期:2012-04-01 00:00:00
abstract::A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and th...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1182540
更新日期:2016-10-01 00:00:00
abstract::This study elucidated the molecular markers that decrease oocyte quality during in vitro culture, restricting optimal developmental potential. Here, we evaluated the transcriptomic differences between cysteamine-treated and non-treated bovine cumulus oocyte complexes (COCs) after 22 h of co-culture in the maturation m...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1706545
更新日期:2020-01-03 00:00:00
abstract::We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.527567
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.630897
更新日期:2012-01-01 00:00:00
abstract::Distinguishing between bull Y- and X-bearing sperm populations is advantageous for techniques using sexed bull semen. The aim of this study was to produce a single-chain fragment variable (scFv) antibody against plasma membrane epitopes on bull Y-bearing sperm. Variable heavy (VH)- and variable light (VL)-region genes...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1811294
更新日期:2020-08-31 00:00:00
abstract::Skeletal myoblast differentiation is controlled by a multitude of transcription factors and signal pathways. Myogenin is a critical transcriptional regulator in the initiation and maintenance of myoblast differentiation. The Akt/FoxO1 signal pathway plays an important role in myoblast differentiation. MicroRNAs are a ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1348357
更新日期:2018-07-03 00:00:00
abstract::Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1777149
更新日期:2020-06-10 00:00:00
abstract::Prolactin is a versatile hormone with multiple activities, including a negative control on egg production. This study was conducted to genotype all the coding portions of the prl gene using PCR-SSCP-sequencing, and to investigate the effects of amino acid substitutions of the prl gene on the structure and function of ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1683568
更新日期:2019-10-29 00:00:00
abstract::P-element-induced wimpy testis-interacting RNAs (piRNAs) are small RNAs that are essential for mammalian spermatogenesis and testicular development. Comparative analyses of the molecular mechanisms of spermatogenesis among different organisms are therefore dependent on accurate piRNA characterizations. In this study, ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1640717
更新日期:2019-07-18 00:00:00
abstract::The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin 1 receptor gene (MC...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-100108341
更新日期:2001-11-01 00:00:00
abstract::The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations foll...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1135807
更新日期:2016-01-01 00:00:00
abstract::Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal m...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1437045
更新日期:2019-01-01 00:00:00
abstract::High concentrate (HC) diet feeding leads to the lysis of rumen microbes and the release of hazardous metabolites, which can trigger inflammatory responses, thereby impairing dairy cow health and production. γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP), which constitutes the peptidoglycan (PGN) layer of bacteria, is ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1870484
更新日期:2021-01-24 00:00:00
abstract::The present study was undertaken to characterize the structure of S100A8 gene and its promoter in water buffalo and yak. Sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete S100A8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. BLAST analys...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.581558
更新日期:2011-07-01 00:00:00
abstract::Since World War II the use of antimicrobial products associated with food animal production has increased. Antimicrobials along with evolving production practices have significantly increased throughput, animal welfare, and improved health. Concerns surrounding the growing significance of emerging and in some cases ra...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390600962274
更新日期:2006-01-01 00:00:00
abstract::Aim of this study was to compare different combinations of penetrating intracellular CPAs, i.e., glycerol (G), ethylene glycol (EG), propylene glycol (PG), dimethyl formamide (DM), and methyl acetamide (MA) and extracellular [egg yolk (EY), egg yolk plasma (EYP), low-density lipoproteins (LDL), and coconut water (CW)]...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1698434
更新日期:2019-12-08 00:00:00
abstract::MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered in model animals. To explore the function and mechanism of miR-1 in goat, we firstly explored the expression profile of miR-1 in goat tissues and cells. Furthermore, the target gene of miR-1 ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1622555
更新日期:2020-10-01 00:00:00
abstract::The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observe...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1242490
更新日期:2017-04-03 00:00:00
abstract::In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their perform...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1329150
更新日期:2018-04-03 00:00:00
abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390802340923
更新日期:2008-01-01 00:00:00
abstract::The expression of genes and their regulation during lactation in Ghezel sheep breed remains less understood. To explore the underlying molecular mechanism of the lactation process in the mammary gland, transcriptome profiles of Iranian fat-tailed Ghezel sheep breed milk at two stages, before (BF) and after peak (AF) s...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1784185
更新日期:2020-07-07 00:00:00
abstract::A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H a...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525921
更新日期:1999-01-01 00:00:00
abstract::Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1198796
更新日期:2017-01-02 00:00:00
abstract::This study was designed to investigate the effect of somatostatin on oocytes maturation and subsequent embryo development in cattle. Bovine granulosa cells separated from oocytes, cultured for 24 h and transfected with pEGFP-N1 vector with mouse SST gene (Experimental) and with out plasmid transfection (Control). RT-P...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390902996723
更新日期:2009-01-01 00:00:00
abstract::Lipopolysaccharide (LPS) is the major pathogen-associated molecular pattern (PAMP) found in the cell wall of gram-negative bacteria and, in mammals, is recognized by the Toll-like receptor 4 (TLR4) in conjunction with the serum protein, lipopolysaccharide-binding protein (LBP), and the CD14 co-receptor. We have found ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390500264896
更新日期:2005-01-01 00:00:00