Alternative splicing of testis-specific lactate dehydrogenase C gene in mammals and pigeon.

Abstract:

:The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice variants of ldhc were observed in the seven species as a result of deletion of one or more exons or insertion of partial sequence of an intron in the mature mRNA. The deleted exons occur mostly in exons 5, 4, 6, and 3. The insertion of a partial sequence of introns, which resulted in an abnormal stop codon in the inserted intron sequence, was observed only in dog and rat. The deletion of exons also resulted in a reading frame shift and formation of a stop codon in some variants. No alternative splicing was observed for ldha and ldhb genes in testis of yak. Native polyacrylamide gel electrophoresis and Western blot analysis revealed no obvious LDH-C4 activity derived from expressed ldhc variants. Our results demonstrated the widespread and unique existence of alternative splicing of ldhc genes in mammals.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Huang L,Lin Y,Jin S,Liu W,Xu Y,Zheng Y

doi

10.1080/10495398.2011.652325

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

114-23

issue

2

eissn

1049-5398

issn

1532-2378

journal_volume

23

pub_type

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