Screening and scanning of single nucleotide polymorphisms in the pig melanocortin 1 receptor gene (MC1R) by pyrosequencing.

abstract：:The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar...

journal_title：Animal biotechnology

authors： Mohalik PK,Sahoo SS,Mishra C,Dash SK,Nayak G

更新日期：2020-02-12 00:00:00

abstract：:A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and th...

journal_title：Animal biotechnology

authors： Wu H,Bi X,Cao F,Zhu C,Liu H,Song J,Ma L,Ma L,Zhang Y,Zhao D,Liu H,Xu X,Zhang S

更新日期：2016-10-01 00:00:00

abstract：:Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...

journal_title：Animal biotechnology

authors： Li Q,Zhao J,Hu W,Wang J,Yu T,Dai Y,Li N

更新日期：2018-04-03 00:00:00

abstract：:Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...

journal_title：Animal biotechnology

authors： Liu W,Lamont SJ

更新日期：2003-05-01 00:00:00

abstract：:The aim of this study was to investigate genes for differential expression in cartilage of foals predisposed to osteochondrosis (OC). Tissue was sampled from the cranial part of the distal intermediate ridge of the tibia in the tarso-crural joint. Foals were considered predisposed to OC when parents had OC at the dist...

journal_title：Animal biotechnology

authors： Austbø L,Røed KH,Dolvik NI,Skretting G

更新日期：2010-04-01 00:00:00

abstract：:Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...

journal_title：Animal biotechnology

authors： Rahimi H,Tukmechi A,Rashidian E

更新日期：2020-06-10 00:00:00

abstract：:Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...

journal_title：Animal biotechnology

authors： Wang Z,Rohrer G,Stone R,Troyer D

更新日期：2000-01-01 00:00:00

abstract：:The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...

journal_title：Animal biotechnology

authors： Rogatcheva MB,Rund LA,Beever JE,Schook LB

更新日期：2003-11-01 00:00:00

abstract：:Fescue toxicosis (FT) reduces beef animal growth and fertility. Animals afflicted with FT typically have decreased circulating prolactin concentrations and thicker summer hair coats. Preliminary experiments examined the informativeness of a novel Dopamine Receptor 2 (DRD2) G/A SNP for resistance to FT. Steers grazed t...

journal_title：Animal biotechnology

authors： Campbell BT,Kojima CJ,Cooper TA,Bastin BC,Wojakiewicz L,Kallenbach RL,Schrick FN,Waller JC

更新日期：2014-01-01 00:00:00

abstract：:The effects of supplementing diet of growing lambs with microbial feed additive mixture (direct-fed microbial; DFM) based on Saccharomyces cerevisiae, lactic acid bacteria and exogenous enzymes on feed intake, nutrient digestibility, nitrogen (N) utilization, ruminal fermentation, blood chemistry and growth performanc...

journal_title：Animal biotechnology

authors： Sallam SMA,Kholif AE,Amin KA,El-Din ANMN,Attia MFA,Matloup OH,Anele UY

更新日期：2020-10-01 00:00:00

abstract：:Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, li...

journal_title：Animal biotechnology

authors： Zahmatkesh A,Ansari Mahyari S,Daliri Joupari M,Rahmani H,Shirazi A,Amiri Roudbar M,Ansari Majd S

更新日期：2016-01-01 00:00:00

abstract：:The caprine Insulin like Growth Factor1 (IGF1) gene was analyzed for identification of single nucleotide polymorphisms (SNPs) and genetic structuring of Indian goat breeds. A panel of 80 samples belonging to nine Indian goat breeds (Capra hircus) including three large sized breeds (Jamunapari, Beetal and Jakhrana); th...

journal_title：Animal biotechnology

authors： Sharma A,Dutt G,Jayakumar S,Saroha V,Verma NK,Dixit SP

更新日期：2013-01-01 00:00:00

abstract：:Skeletal myoblast differentiation is controlled by a multitude of transcription factors and signal pathways. Myogenin is a critical transcriptional regulator in the initiation and maintenance of myoblast differentiation. The Akt/FoxO1 signal pathway plays an important role in myoblast differentiation. MicroRNAs are a ...

journal_title：Animal biotechnology

authors： Zhang S,Chen X,Huang Z,Chen D,Yu B,He J,Zheng P,Yu J,Luo J,Luo Y,Chen H

更新日期：2018-07-03 00:00:00

abstract：:Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP...

journal_title：Animal biotechnology

authors： Yonekura S,Hirota S,Miyazaki H,Tokutake Y

更新日期：2016-01-01 00:00:00

abstract：:Numerous studies have demonstrated that FTO plays an important role in adipogenesis. Herein, we designed a small interfering RNA targeting FTO to knock down its endogenous expression and investigated its effects on the proliferation and differentiation of porcine intramuscular preadipocytes. Its possible mechanism was...

journal_title：Animal biotechnology

authors： Chen X,Luo Y,Jia G,Liu G,Zhao H,Huang Z

更新日期：2017-10-02 00:00:00

abstract：:This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma...

journal_title：Animal biotechnology

authors： Li Y,Yang H,Xia L,Wang S,Bu S

更新日期：2020-03-17 00:00:00

abstract：:To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...

journal_title：Animal biotechnology

authors： Burk NE,Messer LA,Ernst CW,Rothschild MF

更新日期：1998-01-01 00:00:00

abstract：:The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...

journal_title：Animal biotechnology

authors： Fries R,Ewald D,Thaller G,Buitkamp J

更新日期：2001-05-01 00:00:00

abstract：:A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...

journal_title：Animal biotechnology

authors： Su YH,Lin TY,Huang CL,Tu CF,Chuang CK

更新日期：2015-01-01 00:00:00

abstract：:Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...

journal_title：Animal biotechnology

authors： Agca C,Bidwell CA,Donkin SS

更新日期：2004-05-01 00:00:00

abstract：:A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were...

journal_title：Animal biotechnology

authors： Ali SA,Boby N,Preena P,Singh SV,Kaur G,Ghosh SK,Nandi S,Chaudhuri P

更新日期：2021-01-10 00:00:00

abstract：:The objectives of this study were to evaluate the transfection effectiveness of retroviral vector PLNCX2 in yak sperm-mediated gene transfer (SMGT) and the effect of SMGT on sperm motility and fertilizability. Human lactoferrin (hLF) DNA was ligated into PLNCX2 to construct recombinant plasmid PLNCX2-hLF, then, using ...

journal_title：Animal biotechnology

authors： Zi XD,Chen SW,Liang GN,Chen DW,Zhang DW,Yin RH

更新日期：2009-01-01 00:00:00

abstract：:MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered in model animals. To explore the function and mechanism of miR-1 in goat, we firstly explored the expression profile of miR-1 in goat tissues and cells. Furthermore, the target gene of miR-1 ...

journal_title：Animal biotechnology

authors： Sui M,Zheng Q,Wu H,Zhu L,Ling Y,Wang L,Fang F,Liu Y,Zhang Z,Chu M,Zhang Y

更新日期：2020-10-01 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a member of transcriptional coactivator of the peroxisome proliferator-activated receptor. It is involved in lipid metabolism, energy metabolism, adipocyte differentiation and regulation of mitochondrial biogenesis. Therefore, the genet...

journal_title：Animal biotechnology

authors： Chen P,Zhao H,Wu M,He S,Yuan T,Yi X,Liu S,Pan Y,Li Q,Wang S,Sun X

更新日期：2020-08-09 00:00:00

abstract：:The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...

journal_title：Animal biotechnology

authors： Yue B,Han F,Wu J,Wang Y,Zhang C,Fang X,Qi X,Bai Y,Chen H

更新日期：2017-10-02 00:00:00

abstract：:Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...

journal_title：Animal biotechnology

authors： Boudali SF,Al-Jumaili AS,Bouandas A,Mahammi FZ,Tabet Aoul N,Hanotte O,Gaouar SBS

更新日期：2020-08-13 00:00:00

abstract：:The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...

journal_title：Animal biotechnology

authors： Ji S,Willis GM,Scott RR,Spurlock ME

更新日期：1998-01-01 00:00:00

abstract：:Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...

journal_title：Animal biotechnology

authors： Ogorevc J,Poklukar K,Dovč P

更新日期：2019-11-18 00:00:00

abstract：:Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...

journal_title：Animal biotechnology

authors： Shi X,Xia C,Pan B,Wang M

更新日期：2006-01-01 00:00:00

abstract：:Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck SP3 (duSP3). The full-length cDNA sequence of the duSP3 gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, includ...

journal_title：Animal biotechnology

authors： Cao Z,Gao W,Gu T,Huo W,Zhang Y,Zhang Y,Xu Q,Chen G

更新日期：2020-03-17 00:00:00