Abstract:
:Knockdown of myostatin gene (MSTN), transforming growth factor-β superfamily, and a negative regulator of the skeletal muscle growth, by RNA interference (RNAi), has been reported to increase muscle mass in mammals. The current study was aimed to cotransfect two anti-MSTN short hairpin RNA (shRNA) constructs in caprine fetal fibroblast cells for transient silencing of MSTN gene. In the present investigation, approximately 89% MSTN silencing was achieved in transiently transfected caprine fetal fibroblast cells by cotransfection of two best out of four anti-MSTN shRNA constructs. Simultaneously, we also monitored the induction of IFN responsive genes (IFN), pro-apoptotic gene (caspase3) and anti-apoptotic gene (MCL-1) due to cotransfection of different anti-MSTN shRNA constructs. We observed induction of 0.66-19.12, 1.04-4.14, 0.50-3.43, and 0.42-1.98 for folds IFN-β, OAS1, caspase3, and MCL-1 genes, respectively (p < 0.05). This RNAi based cotransfection method could provide an alternative strategy of gene knockout and develop stable caprine fetal fibroblast cells. Furthermore, these stable cells can be used as a cell donor for the development of transgenic cloned embryos by somatic cell nuclear transfer (SCNT) technique.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Hati Boruah JL,Ranjan R,Gogoi H,Pandey SK,Kumar D,Phukan AJ,Bori J,Sarkhel BCdoi
10.1080/10495398.2015.1074915subject
Has Abstractpub_date
2016-01-01 00:00:00pages
44-51issue
1eissn
1049-5398issn
1532-2378journal_volume
27pub_type
杂志文章abstract::The expression of genes and their regulation during lactation in Ghezel sheep breed remains less understood. To explore the underlying molecular mechanism of the lactation process in the mammary gland, transcriptome profiles of Iranian fat-tailed Ghezel sheep breed milk at two stages, before (BF) and after peak (AF) s...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1784185
更新日期:2020-07-07 00:00:00
abstract::The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1437046
更新日期:2019-01-01 00:00:00
abstract::The aim of the study was to introduce a convenient method for identification of differences among individual animals in genes supposed to influence meat performance in pigs. The set of seven candidate genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF, and MC3R) was used. To determine the genotypes, multiplex polymerase chain r...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601088657
更新日期:2007-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1398754
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abstract::Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525939
更新日期:1999-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1723608
更新日期:2020-03-17 00:00:00
abstract:BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.630897
更新日期:2012-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1027774
更新日期:2015-01-01 00:00:00
abstract::The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...
journal_title:Animal biotechnology
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doi:10.1080/10495398.2014.881369
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journal_title:Animal biotechnology
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更新日期:2020-10-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399809525902
更新日期:1998-01-01 00:00:00
abstract::The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations foll...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1135807
更新日期:2016-01-01 00:00:00
abstract::A disintegrin and metalloprotease (ADAM) 12 is a member of the ADAM family possessing a putative role in a variety of biological processes such as modulation of proteolytic processing, cell adhesion, cell fusion, and signaling. Recently, it has been suggested that ADAM12 is involved in regulation of adipogenesis as we...
journal_title:Animal biotechnology
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doi:10.1080/10495390802212445
更新日期:2008-01-01 00:00:00
abstract::Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1777149
更新日期:2020-06-10 00:00:00
abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390802340923
更新日期:2008-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1271805
更新日期:2017-10-02 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390801887361
更新日期:2008-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1433191
更新日期:2019-01-01 00:00:00
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journal_title:Animal biotechnology
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doi:10.1080/10495398.2016.1273835
更新日期:2017-10-02 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.652325
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doi:10.1080/10495390009525945
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journal_title:Animal biotechnology
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更新日期:2013-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1735404
更新日期:2020-04-02 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1828095
更新日期:2020-10-06 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-100108341
更新日期:2001-11-01 00:00:00
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journal_title:Animal biotechnology
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doi:10.1080/10495398.2020.1803892
更新日期:2020-08-13 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.527567
更新日期:2011-01-01 00:00:00
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journal_title:Animal biotechnology
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doi:10.1080/10495398.2015.1102148
更新日期:2016-01-01 00:00:00
abstract::A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1863225
更新日期:2021-01-10 00:00:00