Cytoskeleton Genes Expression and Survival Rate Comparison Between Immature and Mature Yak Oocyte After OPS Vitrification.

Abstract:

:This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Survival rate after vitrification were compared between immature and mature yak oocytes and cytoskeleton-related genes [cytokeratin 8 (CK8), β-actin (ACTB), and gap junction protein, alpha 1 (GJA1)] were tested by real-time PCR. Our results showed that MII stage survival rate after open pulled straw vitrification (35.60%) is significantly higher than GV stage (25.90%) oocytes. Furthermore, expression of CK8, ACTB, and GJA1 in MII stage oocytes are also significantly higher than GV stage oocytes. In conclusion, our study demonstrated that higher expression of GJA1, CK8, and ACTB in vitrify-warmed MII stage oocytes when compared with GV stage oocytes and such discrepancy might result in higher survival rate in vitrify-warmed MII stage oocytes.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Wei X,Sijie Y,Weibin Z,Qing X,Jie Z,Xiangdong Z

doi

10.1080/10495398.2017.1369429

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

247-251

issue

4

eissn

1049-5398

issn

1532-2378

journal_volume

29

pub_type

杂志文章
  • Association of BF, RBP4, and ESR2 Genotypes with Litter Size in an Autochthonous Pig Population.

    abstract::The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observe...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2016.1242490

    authors: Laliotis GP,Marantidis A,Avdi M

    更新日期:2017-04-03 00:00:00

  • Beneficial and adverse effects of medicinal plants as feed supplements in poultry nutrition: a review.

    abstract::Medicinal plants exhibit colossal impact on poultry industries by improving its performance and productivity. However, some of these plants show adverse influence too by decreasing egg production percentage, egg mass, and microbiota counts. Green tea, nettle, pennyroyal, yarrow, and alfalfa in the form of seed, powder...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1798973

    authors: Pliego AB,Tavakoli M,Khusro A,Seidavi A,Elghandour MMMY,Salem AZM,Márquez-Molina O,Rene Rivas-Caceres R

    更新日期:2020-08-03 00:00:00

  • Genetic characterization and assessment of diversity in Pandharpuri buffalo breed of India using heterologous microsatellite markers.

    abstract::The present study was aimed at genetic characterization and diversity analysis of Pandharpuri buffalo population using the Food and Agriculture Organization (FAO) recommended bovine microsatellite markers. Genomic DNA was isolated from blood samples of 50 unrelated animals and a total of 23 microsatellite loci were am...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2019.1612757

    authors: Khade KA,Panigrahi M,Ahmad SF,Kumar P,Bhushan B

    更新日期:2020-10-01 00:00:00

  • Genetic Variants in ADD1 Gene and their Associations with Growth Traits in Cattle.

    abstract::The α-adducin (ADD1) is a subunit of adducin which is a cytoskeleton heterodimeric protein. Adducin participates in oocytes chromosome meiosis of mice, prompting adducin has an effect on embryonic development. Adducin gene mutation has significantly functional change. So the present study was to identify and character...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1398754

    authors: Huang YZ,Qian LN,Wang J,Zhang CL,Fang XT,Lei CZ,Lan XY,Ma Y,Bai YY,Lin FP,Chen H

    更新日期:2019-01-01 00:00:00

  • Bovine Sex Determining Region Y: Cloning, Optimized Expression, and Purification.

    abstract::Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2016.1198796

    authors: Soleymani B,Hafezian SH,Mianji GR,Mansouri K,Chaharaein B,Tajehmiri A,Sharifi Tabar M,Mostafaie A

    更新日期:2017-01-02 00:00:00

  • Simple sequence repeats for genetic studies of alpaca.

    abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390802340923

    authors: Reed KM,Chaves LD

    更新日期:2008-01-01 00:00:00

  • Genomic organization and promoter analysis of the bovine ADAM12 gene.

    abstract::A disintegrin and metalloprotease (ADAM) 12 is a member of the ADAM family possessing a putative role in a variety of biological processes such as modulation of proteolytic processing, cell adhesion, cell fusion, and signaling. Recently, it has been suggested that ADAM12 is involved in regulation of adipogenesis as we...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390802212445

    authors: Taniguchi Y,Doronbekov K,Yamada T,Sasaki Y,Takano A,Sugimoto Y

    更新日期:2008-01-01 00:00:00

  • Next generation semiconductor based-sequencing of a nutrigenetics target gene (GPR120) and association with growth rate in Italian Large White pigs.

    abstract::The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2014.881369

    authors: Fontanesi L,Bertolini F,Scotti E,Schiavo G,Colombo M,Trevisi P,Ribani A,Buttazzoni L,Russo V,Dall'Olio S

    更新日期:2015-01-01 00:00:00

  • Temporal microRNA expression profile of pig peripheral blood during postnatal development.

    abstract::Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this stu...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1824920

    authors: Zhong Z,Zhu X,Tang Q,Hong L,Gu Y,He Z,Tao X,Yang X,Liang Y,Shen L,Tan Y,Zeng K,Ying S,Yang Y,Lei Y,Wang Y,Gong J,Chen X,Zhou R,Zhu L,Lv X

    更新日期:2021-01-18 00:00:00

  • Metabolome fingerprints, performance and carcass quality of beef calves supplemented with antibiotic free additive.

    abstract::The aim of this study was to investigate the effects of probiotics on pre-weaning performance, muscle, and fat deposition and serum metabolite profiles in male and female Senepol calves. Thirty new-born Senepol calves, 15 males and 15 females, were randomly allocated to the following treatments: CON a control group th...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1828095

    authors: Gandra JR,Pedrini CA,Cônsolo NRB,Acosta AP,Seno LO,Barbosa LCGS,Noia IZ,Buarque VLM,Padilla ARH,Colnago LA,Gandra ERS

    更新日期:2020-10-06 00:00:00

  • Identification of sequence tagged sites in the Asian and African elephant.

    abstract::To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495399809525902

    authors: Burk NE,Messer LA,Ernst CW,Rothschild MF

    更新日期:1998-01-01 00:00:00

  • Somatostatin can alter fertility genes expression, oocytes maturation, and embryo development in cattle.

    abstract::This study was designed to investigate the effect of somatostatin on oocytes maturation and subsequent embryo development in cattle. Bovine granulosa cells separated from oocytes, cultured for 24 h and transfected with pEGFP-N1 vector with mouse SST gene (Experimental) and with out plasmid transfection (Control). RT-P...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390902996723

    authors: Moaeen-ud-Din M,Malik N,Yang LG

    更新日期:2009-01-01 00:00:00

  • Genetic structuring of nine Indian domestic goat breeds based on SNPs identified in IGF-1 gene.

    abstract::The caprine Insulin like Growth Factor1 (IGF1) gene was analyzed for identification of single nucleotide polymorphisms (SNPs) and genetic structuring of Indian goat breeds. A panel of 80 samples belonging to nine Indian goat breeds (Capra hircus) including three large sized breeds (Jamunapari, Beetal and Jakhrana); th...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2013.763815

    authors: Sharma A,Dutt G,Jayakumar S,Saroha V,Verma NK,Dixit SP

    更新日期:2013-01-01 00:00:00

  • Evaluation of Bovine High-Density SNP Genotyping Array in Indigenous Dairy Cattle Breeds.

    abstract::In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their perform...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1329150

    authors: Dash S,Singh A,Bhatia AK,Jayakumar S,Sharma A,Singh S,Ganguly I,Dixit SP

    更新日期:2018-04-03 00:00:00

  • Characterization of a normalized cDNA library from bovine intestinal muscle and epithelial tissues.

    abstract::Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/abio-200053398

    authors: Baumann RG,Baldwin RL,Van Tassell CP 6th,Sonstegard TS,Matukumalli LK

    更新日期:2005-01-01 00:00:00

  • Assessment of the nucleotide sequence variability in the bovine T-cell receptor alpha delta joining gene region.

    abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/abio-100102977

    authors: Fries R,Ewald D,Thaller G,Buitkamp J

    更新日期:2001-05-01 00:00:00

  • Molecular evolution, characterization and expression profiling of uterine aldoketoreductase 1B5 gene in endometrium of goat (Capra hircus).

    abstract::Aldoketoreductase 1B5 (AKR1B5), a member of the Aldoketoreductase family, is involved in the production of Prostaglandin F2α (PGF2α) as one of vital prostaglandin F synthase (PGFS). PGs (Prostaglandins) play a crucial role in female reproductive system. In the present study, we cloned and characterized the full-length...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2013.872653

    authors: Kumar R,Ramteke PW,Sharma SK,Mitra A

    更新日期:2015-01-01 00:00:00

  • Construction of a CRISPR-Cas9 System for Pig Genome Targeting.

    abstract::A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2015.1027774

    authors: Su YH,Lin TY,Huang CL,Tu CF,Chuang CK

    更新日期:2015-01-01 00:00:00

  • Polymorphic Microsatellite Markers for Solen grandis and Their Cross-Species Amplification in Three Other Species.

    abstract::The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genet...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1367690

    authors: Nie H,Jiang L,Gao Y,Xu H,Du S,Yan X

    更新日期:2019-01-01 00:00:00

  • Maternal origin and genetic diversity of Algerian domestic chicken (Gallus gallus domesticus) from North-Western Africa based on mitochondrial DNA analysis.

    abstract::Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1803892

    authors: Boudali SF,Al-Jumaili AS,Bouandas A,Mahammi FZ,Tabet Aoul N,Hanotte O,Gaouar SBS

    更新日期:2020-08-13 00:00:00

  • Candidate SNP of CACNA2D1 Gene Associated with Clinical Mastitis and Production Traits in Sahiwal (Bos taurus indicus) and Karan Fries (Bos taurus taurus × Bos taurus indicus).

    abstract::The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2018.1437046

    authors: Magotra A,Gupta ID,Verma A,Alex R,Mr V,Ahmad T

    更新日期:2019-01-01 00:00:00

  • Association of genetic variability in CD209 gene with bovine paratuberculosis disease: a case-control study in the Indian cattle population.

    abstract::This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1823400

    authors: Kumar S,Kumar S,Singh RV,Chauhan A,Kumar A,Bharati J,Singh SV

    更新日期:2020-09-28 00:00:00

  • Molecular Characterization, Polymorphism, and Association of Porcine GADD45G Gene.

    abstract::Growth arrest and DNA-damage-inducible gamma (GADD45G) is a reproduction related gene. In this study, the full-length cDNA sequence of porcine GADD45G gene was cloned through rapid amplification of cDNA ends (RACE) method. The porcine GADD45G gene encodes a protein of 159 amino acids that shares high homology with the...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2015.1005216

    authors: Liu C,Zhang W,Yang D,Liu Y

    更新日期:2015-01-01 00:00:00

  • Interferon-alpha genes from Bos and Bubalus bubalus.

    abstract::Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390500461104

    authors: Shi X,Xia C,Pan B,Wang M

    更新日期:2006-01-01 00:00:00

  • A novel 17 bp InDel polymorphism within the PPARGC1A gene is significantly associated with growth traits in sheep.

    abstract::Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a member of transcriptional coactivator of the peroxisome proliferator-activated receptor. It is involved in lipid metabolism, energy metabolism, adipocyte differentiation and regulation of mitochondrial biogenesis. Therefore, the genet...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1796697

    authors: Chen P,Zhao H,Wu M,He S,Yuan T,Yi X,Liu S,Pan Y,Li Q,Wang S,Sun X

    更新日期:2020-08-09 00:00:00

  • SNaPshot minisequencing and a panel of candidate genes for complex routine testing of meat performance traits in pigs.

    abstract::The aim of the study was to introduce a convenient method for identification of differences among individual animals in genes supposed to influence meat performance in pigs. The set of seven candidate genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF, and MC3R) was used. To determine the genotypes, multiplex polymerase chain r...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390601088657

    authors: Civánová K,Knoll A

    更新日期:2007-01-01 00:00:00

  • Growth, immunity, relative gene expression, carcass traits and economic efficiency of two rabbit breeds fed prebiotic supplemented diets.

    abstract::The current study designed to evaluate the effect of oligosaccharide supplemented diets on growth performance, histomorphometric changes, economic efficiency and genetic expression of some growth and immunity-relative genes. One hundred and twenty weaned male rabbits, six weeks of age of two breeds (NZW and APPRI) wer...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1800485

    authors: Abd El-Aziz AH,El-Kasrawy NI,Abd El-Hack ME,Kamel SZ,Mahrous UE,El-Deeb EM,Atta MS,Amer MS,Naiel MAE,Khafaga AF,Metwally AE,Abo Ghanima MM

    更新日期:2020-07-31 00:00:00

  • Effect of in-straw thawing on in vitro- and in vivo-development of vitrified mouse morulae.

    abstract::For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution (0.5 M Sucrose) an...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390601091040

    authors: Yang ZQ,Zhou GB,Hou YP,Yan CL,Zhu SE

    更新日期:2007-01-01 00:00:00

  • Effect of Co-transfection of Anti-myostatin shRNA Constructs in Caprine Fetal Fibroblast Cells.

    abstract::Knockdown of myostatin gene (MSTN), transforming growth factor-β superfamily, and a negative regulator of the skeletal muscle growth, by RNA interference (RNAi), has been reported to increase muscle mass in mammals. The current study was aimed to cotransfect two anti-MSTN short hairpin RNA (shRNA) constructs in caprin...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2015.1074915

    authors: Hati Boruah JL,Ranjan R,Gogoi H,Pandey SK,Kumar D,Phukan AJ,Bori J,Sarkhel BC

    更新日期:2016-01-01 00:00:00

  • Cloning of the calpain regulatory subunit cDNA from fish reveals a divergent domain-V.

    abstract::Calpains are Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed micro- and m-calpains. Both are heterodimers, consisting of a distinct catalytic subunit and a common regulatory subunit. We describe cloning and sequencing of the calpain small (regulatory) subunit (cpns) cDNA from rain...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/LABT-200036706

    authors: Salem M,Nath J,Killefer J

    更新日期:2004-01-01 00:00:00