Abstract:
:A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies. The array elements were selected from > 7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries. Sequence similarity searches of the 3820 ESTs represented on the array using BLASTN identified 3290 (86.1%) as putative human orthologs, with the remainder consisting of "novel" genes or highly divergent orthologs. Experiments were conducted with a prototype 768 gene microarray created from spleen cDNAs and with the 3800 gene array that included genes from spleen and placenta. The 768 gene array was used to profile RNA transcripts expressed by adult and fetal spleen. The 3800 gene array was used to profile transcripts expressed by adult brain and placenta. Microarray analysis of RNA extracted from fetal and adult spleen identified 29 genes that were differentially expressed two-fold or more. Transcriptional differences of two of these genes, IGJ and CTSS, were confirmed using TaqMan technology. The comparison of brain and placenta revealed 400 genes expressed at higher levels in brain and 72 genes expressed at higher levels in placenta. These results demonstrate the potential power of microarrays for understanding the molecular mechanisms of cattle development, disease resistance, nutrition, fertility and production traits.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Band MR,Olmstead C,Everts RE,Liu ZL,Lewin HAdoi
10.1081/ABIO-120005779keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
163-72issue
1eissn
1049-5398issn
1532-2378journal_volume
13pub_type
杂志文章abstract::Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck SP3 (duSP3). The full-length cDNA sequence of the duSP3 gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, includ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1740240
更新日期:2020-03-17 00:00:00
abstract::The aim of this study was to investigate the effects of probiotics on pre-weaning performance, muscle, and fat deposition and serum metabolite profiles in male and female Senepol calves. Thirty new-born Senepol calves, 15 males and 15 females, were randomly allocated to the following treatments: CON a control group th...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1828095
更新日期:2020-10-06 00:00:00
abstract::A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1027774
更新日期:2015-01-01 00:00:00
abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/abio-100102977
更新日期:2001-05-01 00:00:00
abstract::Knockdown of myostatin gene (MSTN), transforming growth factor-β superfamily, and a negative regulator of the skeletal muscle growth, by RNA interference (RNAi), has been reported to increase muscle mass in mammals. The current study was aimed to cotransfect two anti-MSTN short hairpin RNA (shRNA) constructs in caprin...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1074915
更新日期:2016-01-01 00:00:00
abstract::High concentrate (HC) diet feeding leads to the lysis of rumen microbes and the release of hazardous metabolites, which can trigger inflammatory responses, thereby impairing dairy cow health and production. γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP), which constitutes the peptidoglycan (PGN) layer of bacteria, is ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1870484
更新日期:2021-01-24 00:00:00
abstract::The aim of this study was to investigate genes for differential expression in cartilage of foals predisposed to osteochondrosis (OC). Tissue was sampled from the cranial part of the distal intermediate ridge of the tibia in the tarso-crural joint. Foals were considered predisposed to OC when parents had OC at the dist...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495391003608316
更新日期:2010-04-01 00:00:00
abstract::Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1313269
更新日期:2018-04-03 00:00:00
abstract::This study elucidated the molecular markers that decrease oocyte quality during in vitro culture, restricting optimal developmental potential. Here, we evaluated the transcriptomic differences between cysteamine-treated and non-treated bovine cumulus oocyte complexes (COCs) after 22 h of co-culture in the maturation m...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1706545
更新日期:2020-01-03 00:00:00
abstract::A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and th...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1182540
更新日期:2016-10-01 00:00:00
abstract::The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2014.881369
更新日期:2015-01-01 00:00:00
abstract::Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1102148
更新日期:2016-01-01 00:00:00
abstract::Medicinal plants exhibit colossal impact on poultry industries by improving its performance and productivity. However, some of these plants show adverse influence too by decreasing egg production percentage, egg mass, and microbiota counts. Green tea, nettle, pennyroyal, yarrow, and alfalfa in the form of seed, powder...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1798973
更新日期:2020-08-03 00:00:00
abstract::Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this stu...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1824920
更新日期:2021-01-18 00:00:00
abstract::The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1437046
更新日期:2019-01-01 00:00:00
abstract::The α-adducin (ADD1) is a subunit of adducin which is a cytoskeleton heterodimeric protein. Adducin participates in oocytes chromosome meiosis of mice, prompting adducin has an effect on embryonic development. Adducin gene mutation has significantly functional change. So the present study was to identify and character...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1398754
更新日期:2019-01-01 00:00:00
abstract::The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.652325
更新日期:2012-04-01 00:00:00
abstract::Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120037897
更新日期:2004-05-01 00:00:00
abstract::Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120022136
更新日期:2003-05-01 00:00:00
abstract::In pigs, susceptibility to enterotoxigenic Escherichia coli (ETEC) K88 strains (locus F4bcR) is determined by a dominant allele, with the recessive allele determining resistance. The susceptible allele also appeared to be associated with higher growth rate even with discordant results. A single nucleotide polymorphism...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.653462
更新日期:2012-01-01 00:00:00
abstract::A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1863225
更新日期:2021-01-10 00:00:00
abstract::The caprine Insulin like Growth Factor1 (IGF1) gene was analyzed for identification of single nucleotide polymorphisms (SNPs) and genetic structuring of Indian goat breeds. A panel of 80 samples belonging to nine Indian goat breeds (Capra hircus) including three large sized breeds (Jamunapari, Beetal and Jakhrana); th...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2013.763815
更新日期:2013-01-01 00:00:00
abstract::Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525939
更新日期:1999-01-01 00:00:00
abstract::Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1687091
更新日期:2019-11-18 00:00:00
abstract::The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genet...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1367690
更新日期:2019-01-01 00:00:00
abstract::This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1823400
更新日期:2020-09-28 00:00:00
abstract::The aim of the study was to introduce a convenient method for identification of differences among individual animals in genes supposed to influence meat performance in pigs. The set of seven candidate genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF, and MC3R) was used. To determine the genotypes, multiplex polymerase chain r...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601088657
更新日期:2007-01-01 00:00:00
abstract::The objectives of this study were to evaluate the transfection effectiveness of retroviral vector PLNCX2 in yak sperm-mediated gene transfer (SMGT) and the effect of SMGT on sperm motility and fertilizability. Human lactoferrin (hLF) DNA was ligated into PLNCX2 to construct recombinant plasmid PLNCX2-hLF, then, using ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390903196455
更新日期:2009-01-01 00:00:00
abstract::Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390500461104
更新日期:2006-01-01 00:00:00
abstract::Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1803892
更新日期:2020-08-13 00:00:00