Harvesting the genomic promise: recombineering sequences for phenotypes.

Abstract:

:The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize genes contributing towards phenotypic variation. Whole-genome BAC contigs are also being constructed that will serve as the tiling path for genomic sequencing. Harvesting this genetic information for biological gain requires either genetic selection or the production of genetically modified animals. This later approach when coupled with nuclear transfer technology (NT) provides "clones" of genetically modified animals. However, to date, the production of genetically modified animals has been limited to either microinjection of small gene constructs into embryos with random insertion or complex gene constructs designed to knock-out targeted gene expression. Neither of these approaches provides for introducing directed genetic manipulation allowing for allelic substitution [knock-in], subsequent analyses of gene expression, and cloning. An alternative approach utilizing genomic sequence information and recombineering to direct gene targeting of specific porcine BACs is presented here.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Rogatcheva MB,Rund LA,Beever JE,Schook LB

doi

10.1081/ABIO-120026481

keywords:

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

103-18

issue

2

eissn

1049-5398

issn

1532-2378

journal_volume

14

pub_type

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