Abstract:
:The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genetics. In this study, 10 novel polymorphic microsatellite makers were developed and characterized from the S. grandis through high throughput sequencing. The number of alleles at each locus ranged from 10 to 34 with an average of 20.8 alleles per locus. The observed and expected heterozygosities varied from 0.433 to 1.000 and from 0.696 to 0.976, with an average of 0.793 and 0.884, respectively. The polymorphism information content (PIC) value ranged from 0.633 (Sg43838) to 0.958 (Sg3754), with an average of 0.858. The cross-species amplification transferability of 10 loci to three closely related species ranged from 4.17 to 62.5%. These microsatellite loci will be useful for further investigation of population structure and conversation genetics of this species.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Nie H,Jiang L,Gao Y,Xu H,Du S,Yan Xdoi
10.1080/10495398.2017.1367690subject
Has Abstractpub_date
2019-01-01 00:00:00pages
82-86issue
1eissn
1049-5398issn
1532-2378journal_volume
30pub_type
杂志文章abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390802340923
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journal_title:Animal biotechnology
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doi:10.1080/10495390902996723
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journal_title:Animal biotechnology
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journal_title:Animal biotechnology
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journal_title:Animal biotechnology
pub_type: 杂志文章
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journal_title:Animal biotechnology
pub_type: 杂志文章
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journal_title:Animal biotechnology
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journal_title:Animal biotechnology
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doi:10.1080/10495398.2019.1622555
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journal_title:Animal biotechnology
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1074915
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2012.742909
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399809525902
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journal_title:Animal biotechnology
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journal_title:Animal biotechnology
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