Abstract:
:The spore-forming bacterium Paenibacillus larvae causes a severe and highly infective bee disease, American foulbrood (AFB). Despite the large economic losses induced by AFB, the virulence factors produced by P. larvae are as yet unknown. To identify such virulence factors, we experimentally infected young, susceptible larvae of the honeybee, Apis mellifera carnica, with different P. larvae isolates. Honeybee larvae were reared in vitro in 24-well plates in the laboratory after isolation from the brood comb. We identified genotype-specific differences in the etiopathology of AFB between the tested isolates of P. larvae, which were revealed by differences in the median lethal times. Furthermore, we confirmed that extracts of P. larvae cultures contain low-molecular-weight compounds, which are toxic to honeybee larvae. Our data indicate that P. larvae secretes metabolites into the medium with a potent honeybee toxic activity pointing to a novel pathogenic factor(s) of P. larvae. Genome mining of P. larvae subsp. larvae BRL-230010 led to the identification of several biosynthesis gene clusters putatively involved in natural product biosynthesis, highlighting the potential of P. larvae to produce such compounds.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Schild HA,Fuchs SW,Bode HB,Grünewald Bdoi
10.1128/AEM.04049-13subject
Has Abstractpub_date
2014-04-01 00:00:00pages
2484-92issue
8eissn
0099-2240issn
1098-5336pii
AEM.04049-13journal_volume
80pub_type
杂志文章abstract::To get insight into the limiting factors existing for the efficient production of fungal peroxidase in filamentous fungi, the expression of the Phanerochaete chrysosporium lignin peroxidase H8 (lipA) and manganese peroxidase (MnP) H4 (mnp1) genes in Aspergillus niger has been studied. For this purpose, a protease-defi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.7.3016-3023.2000
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abstract::Laboratory column studies were conducted at the Utah Water Research Laboratory, Logan, Utah, to evaluate reovirus removal from drinking water supplies by slow-rate sand filtration (SSF). Columns, constructed to simulate a full-scale SSF field operation, were inoculated with reovirus at ca. 1,000-times-greater concentr...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.48.4.818-825.1984
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.9.3486-3489.1995
更新日期:1995-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00