Rhodococcus erythropolis BG43 Genes Mediating Pseudomonas aeruginosa Quinolone Signal Degradation and Virulence Factor Attenuation.

Abstract:

:Rhodococcus erythropolis BG43 is able to degrade the Pseudomonas aeruginosa quorum sensing signal molecules PQS (Pseudomonas quinolone signal) [2-heptyl-3-hydroxy-4(1H)-quinolone] and HHQ [2-heptyl-4(1H)-quinolone] to anthranilic acid. Based on the hypothesis that degradation of HHQ might involve hydroxylation to PQS followed by dioxygenolytic cleavage of the heterocyclic ring and hydrolysis of the resulting N-octanoylanthranilate, the genome was searched for corresponding candidate genes. Two gene clusters, aqdA1B1C1 and aqdA2B2C2, each predicted to code for a hydrolase, a flavin monooxygenase, and a dioxygenase related to 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase, were identified on circular plasmid pRLCBG43 of strain BG43. Transcription of all genes was upregulated by PQS, suggesting that both gene clusters code for alkylquinolone-specific catabolic enzymes. An aqdR gene encoding a putative transcriptional regulator, which was also inducible by PQS, is located adjacent to the aqdA2B2C2 cluster. Expression of aqdA2B2C2 in Escherichia coli conferred the ability to degrade HHQ and PQS to anthranilic acid; however, for E. coli transformed with aqdA1B1C1, only PQS degradation was observed. Purification of the recombinant AqdC1 protein verified that it catalyzes the cleavage of PQS to form N-octanoylanthranilic acid and carbon monoxide and revealed apparent Km and kcat values for PQS of ∼27 μM and 21 s(-1), respectively. Heterologous expression of the PQS dioxygenase gene aqdC1 or aqdC2 in P. aeruginosa PAO1 quenched the production of the virulence factors pyocyanin and rhamnolipid and reduced the synthesis of the siderophore pyoverdine. Thus, the toolbox of quorum-quenching enzymes is expanded by new PQS dioxygenases.

journal_name

Appl Environ Microbiol

authors

Müller C,Birmes FS,Rückert C,Kalinowski J,Fetzner S

doi

10.1128/AEM.02145-15

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

7720-9

issue

22

eissn

0099-2240

issn

1098-5336

pii

AEM.02145-15

journal_volume

81

pub_type

杂志文章
  • Only part of the protoxin gene of Bacillus thuringiensis subsp. berliner 1715 is necessary for insecticidal activity.

    abstract::Escherichia coli strains harboring deletion mutations of the insecticidal protoxin gene of Bacillus thuringiensis subsp. berliner 1715 were constructed. Although these strains did not produce intact protoxin, cell extracts from one of the mutants were extremely toxic to tobacco hornworm (Manduca sexta) larvae, indicat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.3.706-708.1985

    authors: Wabiko H,Held GA,Bulla LA Jr

    更新日期:1985-03-01 00:00:00

  • Heterologous Expression of Pseudomonas putida Methyl-Accepting Chemotaxis Proteins Yields Escherichia coli Cells Chemotactic to Aromatic Compounds.

    abstract::Escherichia coli, commonly used in chemotaxis studies, is attracted mostly by amino acids, sugars, and peptides. We envisioned modifying the chemotaxis specificity of E. coli by expressing heterologous chemoreceptors from Pseudomonas putida enabling attraction either to toluene or benzoate. The mcpT gene encoding the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01362-18

    authors: Roggo C,Clerc EE,Hadadi N,Carraro N,Stocker R,van der Meer JR

    更新日期:2018-08-31 00:00:00

  • Mediterranean fruit fly as a potential vector of bacterial pathogens.

    abstract::The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop egg...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.7.4052-4056.2005

    authors: Sela S,Nestel D,Pinto R,Nemny-Lavy E,Bar-Joseph M

    更新日期:2005-07-01 00:00:00

  • Bile salt hydrolase of Bifidobacterium longum-biochemical and genetic characterization.

    abstract::A bile salt hydrolase (BSH) was isolated from Bifidobacterium longum SBT2928, purified, and characterized. Furthermore, we describe for the first time cloning and analysis of the gene encoding BSH (bsh) in a member of the genus Bifidobacterium. The enzyme has a native molecular weight of 125,000 to 130,000 and a subun...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.6.2502-2512.2000

    authors: Tanaka H,Hashiba H,Kok J,Mierau I

    更新日期:2000-06-01 00:00:00

  • Bioconversion of alpha-Damascone by Botrytis cinerea.

    abstract::Bioconversion of alpha-damascone (compound 1) was studied with four strains of Botrytis cinerea in grape must (pH 3.2). As biotransformation products of compound 1, 3-oxo-alpha-damascone, cis- and trans-3-hydroxy-alpha-damascone, gamma-damascenone, 3-oxo-8, 9-dihydro-alpha-damascone, and cis- and trans-3-hydroxy-8,9-d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.1.15-18.1991

    authors: Schoch E,Benda I,Schreier P

    更新日期:1991-01-01 00:00:00

  • Degradation of 1,2-dichloroethane by Ancylobacter aquaticus and other facultative methylotrophs.

    abstract::Cultures of the newly isolated bacterial strains AD20, AD25, and AD27, identified as strains of Ancylobacter aquaticus, were capable of growth on 1,2-dichloroethane (DCE) as the sole carbon and energy source. These strains, as well as two other new DCE utilizers, were facultative methylotrophs and were also able to gr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.3.976-983.1992

    authors: van den Wijngaard AJ,van der Kamp KW,van der Ploeg J,Pries F,Kazemier B,Janssen DB

    更新日期:1992-03-01 00:00:00

  • Purification and Characterization of a Substrate-Size-Recognizing Metalloendopeptidase from Streptococcus cremoris H61.

    abstract::During the ripening of Gouda-type cheese, two kinds of endopeptidases were found to participate in the degradation of alphas1-CN(f1-23), a specific product from alphas1-casein hydrolyzed by chymosin. One of the endopeptidases, lactic acid bacteria endopeptidase (LEP-II), which can recognize the size of its substrates,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.10.2296-2302.1987

    authors: Yan TR,Azuma N,Kaminogawa S,Yamauchi K

    更新日期:1987-10-01 00:00:00

  • Oxidative Pathway for the Biodegradation of Nitrobenzene by Comamonas sp. Strain JS765.

    abstract::Previous studies have shown that the biodegradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45 proceeds by the reduction of nitrobenzene through nitrosobenzene and hydroxylaminobenzene, followed by rearrangement to 2-aminophenol, which then undergoes meta ring cleavage. We report here the isolation of a Com...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.6.2308-2313.1995

    authors: Nishino SF,Spain JC

    更新日期:1995-06-01 00:00:00

  • Ecological behavior of Lactobacillus reuteri 100-23 is affected by mutation of the luxS gene.

    abstract::The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8419-8425.2005

    authors: Tannock GW,Ghazally S,Walter J,Loach D,Brooks H,Cook G,Surette M,Simmers C,Bremer P,Dal Bello F,Hertel C

    更新日期:2005-12-01 00:00:00

  • Selection of clc, cba, and fcb chlorobenzoate-catabolic genotypes from groundwater and surface waters adjacent to the Hyde park, Niagara Falls, chemical landfill.

    abstract::The frequency of isolation of three nonhomologous chlorobenzoate catabolic genotypes (clc, cba, and fcb) was determined for 464 isolates from freshwater sediments and groundwater in the vicinity of the Hyde Park industrial landfill site in the Niagara watershed. Samples were collected from both contaminated and noncon...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.4.1627-1635.1999

    authors: Peel MC,Wyndham RC

    更新日期:1999-04-01 00:00:00

  • Expression of the arginine deiminase pathway genes in Lactobacillus sakei is strain dependent and is affected by the environmental pH.

    abstract::The adaptation of Lactobacillus sakei to a meat environment is reflected in its metabolic potential. For instance, the ability to utilize arginine through the arginine deiminase (ADI) pathway, resulting in additional ATP, represents a competitive benefit. In L. sakei CTC 494, the arc operon (arcABCTDR) shows the same ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07724-11

    authors: Rimaux T,Rivière A,Illeghems K,Weckx S,De Vuyst L,Leroy F

    更新日期:2012-07-01 00:00:00

  • Isolation of halotolerant, thermotolerant, facultative polymer-producing bacteria and characterization of the exopolymer.

    abstract::Over 200 bacterial strains were selected for anaerobic growth at 50 degrees C and extracellular polysaccharide production in a sucrose-mineral salts medium with NaNO(3) and up to 10% NaCl. The predominant cell type was an encapsulated gram-positive, motile, facultative sporeforming rod similar to Bacillus species. Str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.6.1224-1229.1986

    authors: Pfiffner SM,McInerney MJ,Jenneman GE,Knapp RM

    更新日期:1986-06-01 00:00:00

  • Significance of Extracellular Protease for Growth of a Heterotrophic Bacterium, Aeromonas salmonicida.

    abstract::The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid medium (composed of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.4.1031-1037.1985

    authors: Sakai DK

    更新日期:1985-10-01 00:00:00

  • Importance of laccase in vegetative growth of pleurotus Florida.

    abstract::Mycelial culture of Pleurotus florida produced highest extracellular laccase in optimum growth medium. At least two laccases (L(inf1) and L(inf2)) were shown to be present in the culture filtrate. Low-laccase-yielding mutants with impaired L(inf2) activity had poor mycelial growth and could not form fruit body, wherea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.10.4120-4122.1997

    authors: Das N,Sengupta S,Mukherjee M

    更新日期:1997-10-01 00:00:00

  • Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms.

    abstract::We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide so...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02630-07

    authors: Liles MR,Williamson LL,Rodbumrer J,Torsvik V,Goodman RM,Handelsman J

    更新日期:2008-05-01 00:00:00

  • Methods for integrated air sampling and dna analysis for detection of airborne fungal spores.

    abstract::Integrated air sampling and PCR-based methods for detecting airborne fungal spores, using Penicillium roqueforti as a model fungus, are described. P. roqueforti spores were collected directly into Eppendorf tubes using a miniature cyclone-type air sampler. They were then suspended in 0.1% Nonidet P-40, and counted usi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2453-2459.2001

    authors: Williams RH,Ward E,McCartney HA

    更新日期:2001-06-01 00:00:00

  • Viruses as partners in spring bloom microbial trophodynamics.

    abstract::Population sizes of algae, bacteria, heterotrophic flagellates, and viruses were observed through the 1989 spring diatom bloom in Raunefjorden in western Norway. The culmination of the diatom bloom was followed by a peak in the concentration of bacteria and an increase in the concentration of heterotrophic flagellates...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.5.1400-1405.1990

    authors: Bratbak G,Heldal M,Norland S,Thingstad TF

    更新日期:1990-05-01 00:00:00

  • Enhancing the production of hydroxyl radicals by Pleurotus eryngii via quinone redox cycling for pollutant removal.

    abstract::The induction of hydroxyl radical (OH) production via quinone redox cycling in white-rot fungi was investigated to improve pollutant degradation. In particular, we examined the influence of 4-methoxybenzaldehyde (anisaldehyde), Mn(2+), and oxalate on Pleurotus eryngii OH generation. Our standard quinone redox cycling ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02138-08

    authors: Gómez-Toribio V,García-Martín AB,Martínez MJ,Martínez AT,Guillén F

    更新日期:2009-06-01 00:00:00

  • Redox chemistry in laccase-catalyzed oxidation of N-hydroxy compounds.

    abstract::1-Hydroxybenzotriazole, violuric acid, and N-hydroxyacetanilide are three N-OH compounds capable of mediating a range of laccase-catalyzed biotransformations, such as paper pulp delignification and degradation of polycyclic hydrocarbons. The mechanism of their enzymatic oxidation was studied with seven fungal laccases...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.5.2052-2056.2000

    authors: Xu F,Kulys JJ,Duke K,Li K,Krikstopaitis K,Deussen HJ,Abbate E,Galinyte V,Schneider P

    更新日期:2000-05-01 00:00:00

  • Functional screening and in vitro analysis reveal thioesterases with enhanced substrate specificity profiles that improve short-chain fatty acid production in Escherichia coli.

    abstract::Short-chain fatty acid (SCFA) biosynthesis is pertinent to production of biofuels, industrial compounds, and pharmaceuticals from renewable resources. To expand on Escherichia coli SCFA products, we previously implemented a coenzyme A (CoA)-dependent pathway that condenses acetyl-CoA to a diverse group of short-chain ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03303-13

    authors: McMahon MD,Prather KL

    更新日期:2014-02-01 00:00:00

  • Adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and polarized thin films of gold.

    abstract::The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film electrochemical flow cell. The influence of the electrochemical potential was evaluated at two different ionic strengths (0.01 and 0.1 M NaCl; pH...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.7.3188-3194.2001

    authors: Busalmen JP,de Sánchez SR

    更新日期:2001-07-01 00:00:00

  • Disruption of cell-to-cell signaling does not abolish the antagonism of Phaeobacter gallaeciensis toward the fish pathogen Vibrio anguillarum in algal systems.

    abstract::Quorum sensing (QS) regulates Phaeobacter gallaeciensis antagonism in broth systems; however, we demonstrate here that QS is not important for antagonism in algal cultures. QS mutants reduced Vibrio anguillarum to the same extent as the wild type. Consequently, a combination of probiotic Phaeobacter and QS inhibitors ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01436-13

    authors: Prol García MJ,D'Alvise PW,Gram L

    更新日期:2013-09-01 00:00:00

  • Stereoselective Reduction of Ethyl 4-Chloro-3-Oxobutanoate by a Microbial Aldehyde Reductase in an Organic Solvent-Water Diphasic System.

    abstract::Enzyme-catalyzed asymmetric reduction of ethyl 4-chloro-3-oxobutanoate in an organic solvent-water diphasic system was studied. NADPH-dependent aldehyde reductase isolated from Sporobolomyces salmonicolor AKU4429 and glucose dehydrogenase were used as catalysts for reduction of ethyl 4-chloro-3-oxobutanoate and recycl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.8.2374-2377.1990

    authors: Shimizu S,Kataoka M,Katoh M,Morikawa T,Miyoshi T,Yamada H

    更新日期:1990-08-01 00:00:00

  • Plasmid profiles as indicators of the source of contamination of Staphylococcus aureus endemic within poultry processing plants.

    abstract::A total of 530 strains of Staphylococcus aureus were isolated from the defeathering machinery of a chicken processing plant and from neck skin samples of carcasses at different stages of processing in two visits 4 weeks apart. Eleven different plasmid profiles were detected in the isolates, eight being common to both ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.6.1541-1549.1988

    authors: Dodd CE,Chaffey BJ,Waites WM

    更新日期:1988-06-01 00:00:00

  • Temporal changes in microscale colonization of the phylloplane by Aureobasidium pullulans.

    abstract::Colonization of apple leaves by the yeastlike fungus Aureobasidium pullulans was followed quantitatively and spatially at a microscale level throughout two growing seasons. Ten field leaves were sampled on 11 dates in 2003 and 15 dates in 2004. Using an A. pullulans-specific fluorescence in situ hybridization probe an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00744-06

    authors: McGrath MJ,Andrews JH

    更新日期:2006-09-01 00:00:00

  • Anti-Candida albicans activity of Pichia anomala as determined by a growth rate reduction assay.

    abstract::Killer toxin activity of Pichia anomala WC65 appeared fungicidal for P. bimundalis WC38 and fungistatic for Candida albicans RC1. Inhibitory activity against sensitive C. albicans showed a linear relationship between toxin concentrations and the inverse of the reduced growth rates. The plot of toxin concentrations aga...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.5.1099-1103.1988

    authors: Sawant AD,Abdelal AT,Ahearn DG

    更新日期:1988-05-01 00:00:00

  • Culturing enterohemorrhagic Escherichia coli in the presence and absence of glucose as a simple means of evaluating the acid tolerance of stationary-phase cells.

    abstract::Prior growth of seven enterohemorrhagic and one nonenterohemorrhagic strains of Escherichia coli in tryptic soy broth with (TSB+G) and without (TSB-G) 1% glucose was evaluated for its effect on acid tolerance. The final pHs of 18-h TSB+G and TSB-G cultures were 4.6 to 5.2 and 6.9 to 7.0, respectively. Cells were then ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.11.4009-4013.1996

    authors: Buchanan RL,Edelson SG

    更新日期:1996-11-01 00:00:00

  • A nitrite microsensor for profiling environmental biofilms.

    abstract::A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyanocobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 (mu)m. The response is log-linear in freshwater down to 1 (mu)M NO(inf2)(sup-) and in seawater to 10 (mu)M NO(inf2)(sup...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.3.973-977.1997

    authors: De Beer D,Schramm A,Santegoeds CM,Kuhl M

    更新日期:1997-03-01 00:00:00

  • Infectivity titers of adenovirus type 5 suspensions after exposure to cigarette smoke.

    abstract::Suspension of adenovirus type 5 in 2.0 ml of cell culture fluid at 37 degrees C were subjected to smoke from four cigarettes over a 4-h period. The cigarettes were smoked in a normal manner, and the inhaled smoke was exhaled through glass tubing into the virus-containing fluid. The virus suspensions were then titrated...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.36.5.774-775.1978

    authors: Bardell D

    更新日期:1978-11-01 00:00:00

  • Permanent colonization by Lactobacillus casei is hindered by the low rate of cell division in mouse gut.

    abstract::Long residence times of probiotics in the intestinal tract would prolong their potential beneficial health effects and assist colonization. This study investigated the colonization potential of Lactobacillus casei Shirota in mouse intestine by using 5 (and 6)-carboxyfluorescein diacetate, succinimidyl ester (cFDA-SE) ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.2.670-674.2004

    authors: Lee YK,Ho PS,Low CS,Arvilommi H,Salminen S

    更新日期:2004-02-01 00:00:00