Abstract:
:Escherichia coli strains harboring deletion mutations of the insecticidal protoxin gene of Bacillus thuringiensis subsp. berliner 1715 were constructed. Although these strains did not produce intact protoxin, cell extracts from one of the mutants were extremely toxic to tobacco hornworm (Manduca sexta) larvae, indicating that only a part of the protoxin gene is required for insecticidal activity.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Wabiko H,Held GA,Bulla LA Jrdoi
10.1128/AEM.49.3.706-708.1985subject
Has Abstractpub_date
1985-03-01 00:00:00pages
706-8issue
3eissn
0099-2240issn
1098-5336journal_volume
49pub_type
杂志文章abstract::The adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease, shows similar traits to human and animal extraintestinal pathogenic E. coli (ExPEC) with respect to their phylogenetic origin and virulence gene profiles. Here, we demonstrate that animal ExPEC strains generally do...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02614-10
更新日期:2011-08-15 00:00:00
abstract::Various Listeria monocytogenes strains may contaminate a single food product, potentially resulting in simultaneous exposure of consumers to multiple strains. However, due to bias in strain recovery, L. monocytogenes strains isolated from foods by selective enrichment (SE) might not always represent those that can bet...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02120-16
更新日期:2016-12-01 00:00:00
abstract:UNLABELLED:The l-isoaspartyl protein carboxyl methyltransferase (PCM) repairs protein damage resulting from spontaneous conversion of aspartyl or asparaginyl residues to isoaspartate and increases long-term stationary-phase survival of Escherichia coli under stress. In the course of studies intended to examine PCM func...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00623-16
更新日期:2016-08-15 00:00:00
abstract::Ruminococcus albus 8 is a ruminal bacterium capable of metabolizing hemicellulose and cellulose, the major components of the plant cell wall. The enzymes that allow this bacterium to capture energy from the two polysaccharides, therefore, have potential application in plant cell wall depolymerization, a process critic...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00353-11
更新日期:2011-08-01 00:00:00
abstract::The bacterial second messengers (p)ppGpp and bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) regulate important functions, such as transcription, virulence, biofilm formation, and quorum sensing. In mycobacteria, they regulate long-term survival during starvation, pathogenicity, and dormancy. Recently, a Pseudomonas aerugin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03999-14
更新日期:2015-04-01 00:00:00
abstract::The selective incorporation of precursors specific for individual fatty acids in germinating and outgrowing spores of Bacillus thuringiensis is described. The specific precursors utilized were [C]butyrate, -isobutyrate, -valerate, and -isovalerate, which were incorporated into even-numbered normal-chain isomers, even-...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.40.1.166-168.1980
更新日期:1980-07-01 00:00:00
abstract::To understand the continuous problems that Escherichia coli O157:H7 causes as food pathogen, this study assessed global gene regulation in bacteria growing on meat. Since FlhD/FlhC of E. coli K-12 laboratory strains was previously established as a major control point in transducing signals from the environment to seve...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00069-11
更新日期:2011-06-01 00:00:00
abstract::Investigations concerning the role of distribution system biofilms on water quality were conducted at a drinking water utility in New Jersey. The utility experienced long-term bacteriological problems in the distribution system, while treatment plant effluents were uniformly negative for coliform bacteria. Results of ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.12.2714-2724.1987
更新日期:1987-12-01 00:00:00
abstract::Metabolic engineering and multisubunit protein production necessitate the expression of multiple genes at coordinated levels. In bacteria, genes for multisubunit proteins or metabolic pathways are often expressed in operons under the control of a single promoter; expression of the genes is coordinated by varying trans...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.12.5399-5405.2000
更新日期:2000-12-01 00:00:00
abstract::Studies of newly emerged Apis mellifera worker bees have demonstrated that their guts are colonized by a consistent core microbiota within several days of eclosure. We conducted experiments aimed at illuminating the transmission routes and spatiotemporal colonization dynamics of this microbiota. Experimental groups of...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01861-14
更新日期:2014-12-01 00:00:00
abstract::Achromobacter spp. are nonfermentative Gram-negative bacilli considered emergent pathogens in cystic fibrosis (CF). Although some cross-transmission events between CF patients have been described, Achromobacter strains were mostly patient specific, suggesting sporadic acquisitions from nonhuman reservoirs. However, so...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01739-18
更新日期:2018-11-15 00:00:00
abstract::Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.2.560-562.1990
更新日期:1990-02-01 00:00:00
abstract::O island 48 (OI-48) of Escherichia coli consists of three functional gene clusters that encode urease, tellurite resistance (Te(r)), and putative adhesins Iha and AIDA-1. The functions of these clusters in enterohemorrhagic E. coli (EHEC) O157:H7 infection are unknown. Deletion mutants for these three regions were con...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00507-09
更新日期:2009-09-01 00:00:00
abstract::Copper appears to be influencing the distribution and abundance of phytoplankton in marine environments, and cyanobacteria are thought to be the most sensitive of the phytoplankton groups to copper toxicity. By using growth assays of phylogenetically divergent clades, we found that coastal strains of marine Synechococ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00271-09
更新日期:2009-08-01 00:00:00
abstract::The human gut is host to a diversity of microorganisms, including the single-celled microbial eukaryote Blastocystis. Research has shown that most carriers host a single Blastocystis subtype (ST), which is unusual given the considerable within-host species diversity observed for other microbial genera in this ecosyste...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00520-15
更新日期:2015-06-15 00:00:00
abstract::The key enzymes and pathways involved in polyhydroxyalkanoate (PHA) biosynthesis in haloarchaea have been identified in recent years, but the haloarchaeal enzymes for PHA degradation remain unknown. In this study, a patatin-like PHA depolymerase, PhaZh1, was determined to be located on the PHA granules in the haloarch...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.04269-14
更新日期:2015-05-01 00:00:00
abstract::Pseudomonas syringae pv. actinidiae is a reemerging pathogen which causes bacterial canker of kiwifruit (Actinidia sp.). Since 2008, a global outbreak of P. syringae pv. actinidiae has occurred, and in 2010 this pathogen was detected in New Zealand. The economic impact and the development of resistance in P. syringae ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00062-14
更新日期:2014-04-01 00:00:00
abstract::Between November 2002 and April 2003, 244 bottles and cartons of commercially pasteurized cow's milk were obtained at random from retail outlets throughout the Czech Republic. During the same period, samples of raw milk and of milk that was subsequently subjected to a minimum of 71.7 degrees C for 15 s in a local past...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.3.1210-1214.2005
更新日期:2005-03-01 00:00:00
abstract::This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by serRK, which is believed to control the expression of the ser(2003) locus in Bifidobacterium breve UCC2003. The ser(2003) locus consists of two genes, Bbr_1319 (sagA) and Bbr_1320 (serU), whic...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01776-12
更新日期:2012-10-01 00:00:00
abstract::Pseudomonas aeruginosa and Vibrio cholerae showed a strong positive chemotactic response towards rum distillery wastewaters (mostos) and a high oxygen uptake rate in the presence of this complex substrate. Rum slops stimulated only motility in Aeromonas hydrophila and Escherichia coli. The A. hydrophila and E. coli is...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.6.1438-1441.1983
更新日期:1983-12-01 00:00:00
abstract::As a result of the recently proposed mandatory groundwater disinfection requirements to inactivate viruses in potable water supplies, there has been increasing interest in virus fate and transport in the subsurface. Several models have been developed to predict the fate of viruses in groundwater, but few include trans...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.5.1609-1616.1992
更新日期:1992-05-01 00:00:00
abstract::Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.10.3972-3977.1997
更新日期:1997-10-01 00:00:00
abstract::The growth of marine bacteria under iron-limited conditions was investigated. Neither siderophore production nor bacterial growth was detected for Pelagiobacter sp. strain V0110 when Fe(III) was present in the culture medium at a concentration of <1.0 microM. However, the growth of V0110 was strongly stimulated by the...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.7.2797-2803.2000
更新日期:2000-07-01 00:00:00
abstract::The presence of human-pathogenic, enteric bacteria on the surface and in the interior of raw produce is a significant health concern. Several aspects of the biology of the interaction between these bacteria and alfalfa (Medicago sativa) seedlings are addressed here. A collection of enteric bacteria associated with alf...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.3.1783-1790.2003
更新日期:2003-03-01 00:00:00
abstract::In order to understand the role of the mar locus in Salmonella with regard to multiple antibiotic resistance, cyclohexane resistance, and outer membrane protein F (OmpF) regulation, a marA::gfp reporter mutant was constructed in an antibiotic-sensitive Salmonella enterica serovar Typhimurium DT104 background. Salicyla...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.3.1190-1197.2001
更新日期:2001-03-01 00:00:00
abstract::Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple sy...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00239-15
更新日期:2015-06-01 00:00:00
abstract::Bacillus thuringiensis three-domain Cry toxins kill insects by forming pores in the apical membrane of larval midgut cells. Oligomerization of the toxin is an important step for pore formation. Domain I helix α-3 participates in toxin oligomerization. Here we identify an intramolecular salt bridge within helix α-3 of ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01515-17
更新日期:2017-09-29 00:00:00
abstract::We constructed a 7.9-kilobase-pair recombinant shuttle plasmid, designated pHR106, by combining desired segments of three plasmids: an Escherichia coli plasmid (pSL100) which provides a multiple cloning site, a Clostridium perfringens plasmid (pJU122) which provides a clostridial origin of replication, and an E. coli ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.1.268-270.1988
更新日期:1988-01-01 00:00:00
abstract::Resistance to vancomycin permitted detection, in a culture of Streptococcus cremoris 290PC, of a contaminant gram-positive coccus. Morphological and physiological characteristics indicated that this bacterium was a strain of Leuconostoc sp., designated PO184. This strain contained four plasmid species, which were dist...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.6.1129-1133.1984
更新日期:1984-12-01 00:00:00
abstract::A radioisotope method was devised to study bacterial respiratory reduction of arsenate in sediments. The following two arsenic-rich soda lakes in California were chosen for comparison on the basis of their different salinities: Mono Lake (approximately 90 g/liter) and Searles Lake (approximately 340 g/liter). Profiles...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01066-06
更新日期:2006-10-01 00:00:00