Only part of the protoxin gene of Bacillus thuringiensis subsp. berliner 1715 is necessary for insecticidal activity.

Abstract:

:Escherichia coli strains harboring deletion mutations of the insecticidal protoxin gene of Bacillus thuringiensis subsp. berliner 1715 were constructed. Although these strains did not produce intact protoxin, cell extracts from one of the mutants were extremely toxic to tobacco hornworm (Manduca sexta) larvae, indicating that only a part of the protoxin gene is required for insecticidal activity.

journal_name

Appl Environ Microbiol

authors

Wabiko H,Held GA,Bulla LA Jr

doi

10.1128/AEM.49.3.706-708.1985

subject

Has Abstract

pub_date

1985-03-01 00:00:00

pages

706-8

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

49

pub_type

杂志文章
  • Adherent-invasive Escherichia coli phenotype displayed by intestinal pathogenic E. coli strains from cats, dogs, and swine.

    abstract::The adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease, shows similar traits to human and animal extraintestinal pathogenic E. coli (ExPEC) with respect to their phylogenetic origin and virulence gene profiles. Here, we demonstrate that animal ExPEC strains generally do...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02614-10

    authors: Martinez-Medina M,Garcia-Gil J,Barnich N,Wieler LH,Ewers C

    更新日期:2011-08-15 00:00:00

  • Listeria monocytogenes Strains Underrepresented during Selective Enrichment with an ISO Method Might Dominate during Passage through Simulated Gastric Fluid and In Vitro Infection of Caco-2 Cells.

    abstract::Various Listeria monocytogenes strains may contaminate a single food product, potentially resulting in simultaneous exposure of consumers to multiple strains. However, due to bias in strain recovery, L. monocytogenes strains isolated from foods by selective enrichment (SE) might not always represent those that can bet...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02120-16

    authors: Zilelidou E,Karmiri CV,Zoumpopoulou G,Mavrogonatou E,Kletsas D,Tsakalidou E,Papadimitriou K,Drosinos E,Skandamis P

    更新日期:2016-12-01 00:00:00

  • (p)ppGpp-Dependent Persisters Increase the Fitness of Escherichia coli Bacteria Deficient in Isoaspartyl Protein Repair.

    abstract:UNLABELLED:The l-isoaspartyl protein carboxyl methyltransferase (PCM) repairs protein damage resulting from spontaneous conversion of aspartyl or asparaginyl residues to isoaspartate and increases long-term stationary-phase survival of Escherichia coli under stress. In the course of studies intended to examine PCM func...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00623-16

    authors: VandenBerg KE,Ahn S,Visick JE

    更新日期:2016-08-15 00:00:00

  • Biochemical analyses of multiple endoxylanases from the rumen bacterium Ruminococcus albus 8 and their synergistic activities with accessory hemicellulose-degrading enzymes.

    abstract::Ruminococcus albus 8 is a ruminal bacterium capable of metabolizing hemicellulose and cellulose, the major components of the plant cell wall. The enzymes that allow this bacterium to capture energy from the two polysaccharides, therefore, have potential application in plant cell wall depolymerization, a process critic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00353-11

    authors: Moon YH,Iakiviak M,Bauer S,Mackie RI,Cann IK

    更新日期:2011-08-01 00:00:00

  • Novel functions of (p)ppGpp and Cyclic di-GMP in mycobacterial physiology revealed by phenotype microarray analysis of wild-type and isogenic strains of Mycobacterium smegmatis.

    abstract::The bacterial second messengers (p)ppGpp and bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) regulate important functions, such as transcription, virulence, biofilm formation, and quorum sensing. In mycobacteria, they regulate long-term survival during starvation, pathogenicity, and dormancy. Recently, a Pseudomonas aerugin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03999-14

    authors: Gupta KR,Kasetty S,Chatterji D

    更新日期:2015-04-01 00:00:00

  • Incorporation of Specific Fatty Acid Precursors During Spore Germination and Outgrowth in Bacillus thuringiensis.

    abstract::The selective incorporation of precursors specific for individual fatty acids in germinating and outgrowing spores of Bacillus thuringiensis is described. The specific precursors utilized were [C]butyrate, -isobutyrate, -valerate, and -isovalerate, which were incorporated into even-numbered normal-chain isomers, even-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.1.166-168.1980

    authors: Nickerson KW,Bulla LA

    更新日期:1980-07-01 00:00:00

  • Regulation of cell division, biofilm formation, and virulence by FlhC in Escherichia coli O157:H7 grown on meat.

    abstract::To understand the continuous problems that Escherichia coli O157:H7 causes as food pathogen, this study assessed global gene regulation in bacteria growing on meat. Since FlhD/FlhC of E. coli K-12 laboratory strains was previously established as a major control point in transducing signals from the environment to seve...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00069-11

    authors: Sule P,Horne SM,Logue CM,Prüss BM

    更新日期:2011-06-01 00:00:00

  • Examination and characterization of distribution system biofilms.

    abstract::Investigations concerning the role of distribution system biofilms on water quality were conducted at a drinking water utility in New Jersey. The utility experienced long-term bacteriological problems in the distribution system, while treatment plant effluents were uniformly negative for coliform bacteria. Results of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.12.2714-2724.1987

    authors: LeChevallier MW,Babcock TM,Lee RG

    更新日期:1987-12-01 00:00:00

  • Coordinated, differential expression of two genes through directed mRNA cleavage and stabilization by secondary structures.

    abstract::Metabolic engineering and multisubunit protein production necessitate the expression of multiple genes at coordinated levels. In bacteria, genes for multisubunit proteins or metabolic pathways are often expressed in operons under the control of a single promoter; expression of the genes is coordinated by varying trans...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.12.5399-5405.2000

    authors: Smolke CD,Carrier TA,Keasling JD

    更新日期:2000-12-01 00:00:00

  • Routes of Acquisition of the Gut Microbiota of the Honey Bee Apis mellifera.

    abstract::Studies of newly emerged Apis mellifera worker bees have demonstrated that their guts are colonized by a consistent core microbiota within several days of eclosure. We conducted experiments aimed at illuminating the transmission routes and spatiotemporal colonization dynamics of this microbiota. Experimental groups of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01861-14

    authors: Powell JE,Martinson VG,Urban-Mead K,Moran NA

    更新日期:2014-12-01 00:00:00

  • Chronic Airway Colonization by Achromobacter xylosoxidans in Cystic Fibrosis Patients Is Not Sustained by Their Domestic Environment.

    abstract::Achromobacter spp. are nonfermentative Gram-negative bacilli considered emergent pathogens in cystic fibrosis (CF). Although some cross-transmission events between CF patients have been described, Achromobacter strains were mostly patient specific, suggesting sporadic acquisitions from nonhuman reservoirs. However, so...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01739-18

    authors: Dupont C,Jumas-Bilak E,Doisy C,Aujoulat F,Chiron R,Marchandin H

    更新日期:2018-11-15 00:00:00

  • Kinetics of sulfur oxidation at suboptimal temperatures.

    abstract::Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.2.560-562.1990

    authors: Ahonen L,Tuovinen OH

    更新日期:1990-02-01 00:00:00

  • Contributions of O island 48 to adherence of enterohemorrhagic Escherichia coli O157:H7 to epithelial cells in vitro and in ligated pig ileal loops.

    abstract::O island 48 (OI-48) of Escherichia coli consists of three functional gene clusters that encode urease, tellurite resistance (Te(r)), and putative adhesins Iha and AIDA-1. The functions of these clusters in enterohemorrhagic E. coli (EHEC) O157:H7 infection are unknown. Deletion mutants for these three regions were con...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00507-09

    authors: Yin X,Wheatcroft R,Chambers JR,Liu B,Zhu J,Gyles CL

    更新日期:2009-09-01 00:00:00

  • Coastal strains of marine Synechococcus species exhibit increased tolerance to copper shock and a distinctive transcriptional response relative to those of open-ocean strains.

    abstract::Copper appears to be influencing the distribution and abundance of phytoplankton in marine environments, and cyanobacteria are thought to be the most sensitive of the phytoplankton groups to copper toxicity. By using growth assays of phylogenetically divergent clades, we found that coastal strains of marine Synechococ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00271-09

    authors: Stuart RK,Dupont CL,Johnson DA,Paulsen IT,Palenik B

    更新日期:2009-08-01 00:00:00

  • Development and Application of a Blastocystis Subtype-Specific PCR Assay Reveals that Mixed-Subtype Infections Are Common in a Healthy Human Population.

    abstract::The human gut is host to a diversity of microorganisms, including the single-celled microbial eukaryote Blastocystis. Research has shown that most carriers host a single Blastocystis subtype (ST), which is unusual given the considerable within-host species diversity observed for other microbial genera in this ecosyste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00520-15

    authors: Scanlan PD,Stensvold CR,Cotter PD

    更新日期:2015-06-15 00:00:00

  • A patatin-like protein associated with the polyhydroxyalkanoate (PHA) granules of Haloferax mediterranei acts as an efficient depolymerase in the degradation of native PHA.

    abstract::The key enzymes and pathways involved in polyhydroxyalkanoate (PHA) biosynthesis in haloarchaea have been identified in recent years, but the haloarchaeal enzymes for PHA degradation remain unknown. In this study, a patatin-like PHA depolymerase, PhaZh1, was determined to be located on the PHA granules in the haloarch...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04269-14

    authors: Liu G,Hou J,Cai S,Zhao D,Cai L,Han J,Zhou J,Xiang H

    更新日期:2015-05-01 00:00:00

  • Identification of bacteriophages for biocontrol of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae.

    abstract::Pseudomonas syringae pv. actinidiae is a reemerging pathogen which causes bacterial canker of kiwifruit (Actinidia sp.). Since 2008, a global outbreak of P. syringae pv. actinidiae has occurred, and in 2010 this pathogen was detected in New Zealand. The economic impact and the development of resistance in P. syringae ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00062-14

    authors: Frampton RA,Taylor C,Holguín Moreno AV,Visnovsky SB,Petty NK,Pitman AR,Fineran PC

    更新日期:2014-04-01 00:00:00

  • Mycobacterium avium subspecies paratuberculosis cultured from locally and commercially pasteurized cow's milk in the Czech Republic.

    abstract::Between November 2002 and April 2003, 244 bottles and cartons of commercially pasteurized cow's milk were obtained at random from retail outlets throughout the Czech Republic. During the same period, samples of raw milk and of milk that was subsequently subjected to a minimum of 71.7 degrees C for 15 s in a local past...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.3.1210-1214.2005

    authors: Ayele WY,Svastova P,Roubal P,Bartos M,Pavlik I

    更新日期:2005-03-01 00:00:00

  • A two-component regulatory system controls autoregulated serpin expression in Bifidobacterium breve UCC2003.

    abstract::This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by serRK, which is believed to control the expression of the ser(2003) locus in Bifidobacterium breve UCC2003. The ser(2003) locus consists of two genes, Bbr_1319 (sagA) and Bbr_1320 (serU), whic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01776-12

    authors: Alvarez-Martin P,O'Connell Motherway M,Turroni F,Foroni E,Ventura M,van Sinderen D

    更新日期:2012-10-01 00:00:00

  • Bacterial chemotaxis to effluent from a rum distillery in tropical near-shore coastal waters.

    abstract::Pseudomonas aeruginosa and Vibrio cholerae showed a strong positive chemotactic response towards rum distillery wastewaters (mostos) and a high oxygen uptake rate in the presence of this complex substrate. Rum slops stimulated only motility in Aeromonas hydrophila and Escherichia coli. The A. hydrophila and E. coli is...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.6.1438-1441.1983

    authors: Fuentes FA,Biamon EJ,Hazen TC

    更新日期:1983-12-01 00:00:00

  • VIRTUS, a model of virus transport in unsaturated soils.

    abstract::As a result of the recently proposed mandatory groundwater disinfection requirements to inactivate viruses in potable water supplies, there has been increasing interest in virus fate and transport in the subsurface. Several models have been developed to predict the fate of viruses in groundwater, but few include trans...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.5.1609-1616.1992

    authors: Yates MV,Ouyang Y

    更新日期:1992-05-01 00:00:00

  • Induced Natural Transformation of Acinetobacter calcoaceticus in Soil Microcosms.

    abstract::Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.10.3972-3977.1997

    authors: Nielsen KM,Bones AM,Van Elsas JD

    更新日期:1997-10-01 00:00:00

  • Bacterial growth stimulation with exogenous siderophore and synthetic N-acyl homoserine lactone autoinducers under iron-limited and low-nutrient conditions.

    abstract::The growth of marine bacteria under iron-limited conditions was investigated. Neither siderophore production nor bacterial growth was detected for Pelagiobacter sp. strain V0110 when Fe(III) was present in the culture medium at a concentration of <1.0 microM. However, the growth of V0110 was strongly stimulated by the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.7.2797-2803.2000

    authors: Guan LL,Onuki H,Kamino K

    更新日期:2000-07-01 00:00:00

  • Kinetics and strain specificity of rhizosphere and endophytic colonization by enteric bacteria on seedlings of Medicago sativa and Medicago truncatula.

    abstract::The presence of human-pathogenic, enteric bacteria on the surface and in the interior of raw produce is a significant health concern. Several aspects of the biology of the interaction between these bacteria and alfalfa (Medicago sativa) seedlings are addressed here. A collection of enteric bacteria associated with alf...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1783-1790.2003

    authors: Dong Y,Iniguez AL,Ahmer BM,Triplett EW

    更新日期:2003-03-01 00:00:00

  • Multiple antibiotic resistance (mar) locus in Salmonella enterica serovar typhimurium DT104.

    abstract::In order to understand the role of the mar locus in Salmonella with regard to multiple antibiotic resistance, cyclohexane resistance, and outer membrane protein F (OmpF) regulation, a marA::gfp reporter mutant was constructed in an antibiotic-sensitive Salmonella enterica serovar Typhimurium DT104 background. Salicyla...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.3.1190-1197.2001

    authors: Randall LP,Woodward MJ

    更新日期:2001-03-01 00:00:00

  • Polyphenolic extract from maple syrup potentiates antibiotic susceptibility and reduces biofilm formation of pathogenic bacteria.

    abstract::Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple sy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00239-15

    authors: Maisuria VB,Hosseinidoust Z,Tufenkji N

    更新日期:2015-06-01 00:00:00

  • An Intramolecular Salt Bridge in Bacillus thuringiensis Cry4Ba Toxin Is Involved in the Stability of Helix α-3, Which Is Needed for Oligomerization and Insecticidal Activity.

    abstract::Bacillus thuringiensis three-domain Cry toxins kill insects by forming pores in the apical membrane of larval midgut cells. Oligomerization of the toxin is an important step for pore formation. Domain I helix α-3 participates in toxin oligomerization. Here we identify an intramolecular salt bridge within helix α-3 of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01515-17

    authors: Pacheco S,Gómez I,Sánchez J,García-Gómez BI,Soberón M,Bravo A

    更新日期:2017-09-29 00:00:00

  • Development of a new shuttle plasmid system for Escherichia coli and Clostridium perfringens.

    abstract::We constructed a 7.9-kilobase-pair recombinant shuttle plasmid, designated pHR106, by combining desired segments of three plasmids: an Escherichia coli plasmid (pSL100) which provides a multiple cloning site, a Clostridium perfringens plasmid (pJU122) which provides a clostridial origin of replication, and an E. coli ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.1.268-270.1988

    authors: Roberts I,Holmes WM,Hylemon PB

    更新日期:1988-01-01 00:00:00

  • Common occurrence of plasmid DNA and vancomycin resistance in Leuconostoc spp.

    abstract::Resistance to vancomycin permitted detection, in a culture of Streptococcus cremoris 290PC, of a contaminant gram-positive coccus. Morphological and physiological characteristics indicated that this bacterium was a strain of Leuconostoc sp., designated PO184. This strain contained four plasmid species, which were dist...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.6.1129-1133.1984

    authors: Orberg PK,Sandine WE

    更新日期:1984-12-01 00:00:00

  • Dissimilatory arsenate and sulfate reduction in sediments of two hypersaline, arsenic-rich soda lakes: Mono and Searles Lakes, California.

    abstract::A radioisotope method was devised to study bacterial respiratory reduction of arsenate in sediments. The following two arsenic-rich soda lakes in California were chosen for comparison on the basis of their different salinities: Mono Lake (approximately 90 g/liter) and Searles Lake (approximately 340 g/liter). Profiles...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01066-06

    authors: Kulp TR,Hoeft SE,Miller LG,Saltikov C,Murphy JN,Han S,Lanoil B,Oremland RS

    更新日期:2006-10-01 00:00:00