A two-component regulatory system controls autoregulated serpin expression in Bifidobacterium breve UCC2003.

Abstract:

:This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by serRK, which is believed to control the expression of the ser(2003) locus in Bifidobacterium breve UCC2003. The ser(2003) locus consists of two genes, Bbr_1319 (sagA) and Bbr_1320 (serU), which are predicted to encode a hypothetical membrane-associated protein and a serpin-like protein, respectively. The response regulator SerR was shown to bind to the promoter region of ser(2003), and the probable recognition sequence of SerR was determined by a combinatorial approach of in vitro site-directed mutagenesis coupled to transcriptional fusion and electrophoretic mobility shift assays (EMSAs). The importance of the serRK 2CRS in the response of B. breve to protease-mediated induction was confirmed by generating a B. breve serR insertion mutant, which was shown to exhibit altered ser(2003) transcriptional induction patterns compared to the parent strain, UCC2003. Interestingly, the analysis of a B. breve serU mutant revealed that the SerRK signaling pathway appears to include a SerU-dependent autoregulatory loop.

journal_name

Appl Environ Microbiol

authors

Alvarez-Martin P,O'Connell Motherway M,Turroni F,Foroni E,Ventura M,van Sinderen D

doi

10.1128/AEM.01776-12

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

7032-41

issue

19

eissn

0099-2240

issn

1098-5336

pii

AEM.01776-12

journal_volume

78

pub_type

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