Abstract:
:A polymerase chain reaction (PCR) method designed to sensitively detect and identify Campylobacter jejuni and Campylobacter coli without the need for isolating and culturing strains is described. The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or seminested primer approach. A total of 50 bacterial strains, 27 of C. jejuni and C. coli and 23 of other species, were tested, giving no false-positive or false-negative results. The detection limit as determined by ethidium bromide staining of amplification products on agarose gels was 10 bacteria or less in artificially contaminated water, milk, and soft cheese samples with the seminested primer PCR assay. As an application of the PCR system, a set of 93 samples of milk and other dairy products was screened for the presence of C. jejuni and C. coli. We identified six positive samples (6.5%), while none were found with a conventional culture method.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Wegmüller B,Lüthy J,Candrian Udoi
10.1128/AEM.59.7.2161-2165.1993subject
Has Abstractpub_date
1993-07-01 00:00:00pages
2161-5issue
7eissn
0099-2240issn
1098-5336journal_volume
59pub_type
杂志文章abstract::Understanding of functional diversity of microbial populations has lagged description of their molecular diversity. Differences in substrate specificity, kinetics, products, and regulation can dramatically influence phenotypic variation among closely related strains, features that are missed when the strains studied a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01905-13
更新日期:2013-11-01 00:00:00
abstract::Cytolethal distending toxins (CDT) are potent cytotoxins of several Gram-negative pathogenic bacteria, including Escherichia coli, in which five types (CDT-I to CDT-V) have been identified so far. CDT-V is frequently associated with Shiga-toxigenic E. coli (STEC), enterohemorrhagic E. coli (EHEC) O157 strains, and str...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01134-13
更新日期:2013-08-01 00:00:00
abstract::Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.12.4993-4995.1997
更新日期:1997-12-01 00:00:00
abstract::Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cyto...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.9.4168-4171.2000
更新日期:2000-09-01 00:00:00
abstract::Flavonoids are 15-carbon plant secondary metabolites exuded in the rhizosphere that hosts several flavonoid-degrading bacteria. We studied flavonoid catabolism in a plant growth-promoting rhizobacterial strain of Pseudomonas by using a combination of biochemical and genetic approaches. Transposants carrying mini-Tn5gf...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.1.143-151.2002
更新日期:2002-01-01 00:00:00
abstract::To determine the presence of Shiga toxin-producing Escherichia coli (STEC) and other potentially diarrheagenic E. coli strains in retail meats, 7,258 E. coli isolates collected by the U.S. National Antimicrobial Resistance Monitoring System (NARMS) retail meat program from 2002 to 2007 were screened for Shiga toxin ge...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01968-09
更新日期:2010-03-01 00:00:00
abstract::Indirect pathogenicity (IP), the commensal protection of antibiotic-sensitive pathogens by resistant microorganisms of low intrinsic virulence, can prevent the eradication of polymicrobial infections. The contributions of antibiotic resistance mechanisms and biofilm structure to IP within polymicrobial biofilms were i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02474-05
更新日期:2006-07-01 00:00:00
abstract::In all purification stages of a biological sewage treatment plant, phototrophic bacteria were detected by the method of viable cell counts. The predominant species identified belonged to the genus Rhodopseudomonas of purple nonsulfur bacteria. The number of phototrophic bacteria was highest in wastewater containing sl...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.35.1.38-44.1978
更新日期:1978-01-01 00:00:00
abstract::Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total ae...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.9.2771-2775.1992
更新日期:1992-09-01 00:00:00
abstract::This work presents data on the application of a bacterial luciferase used to monitor gene expression of Streptococcus thermophilus in the digestive tract. The main result is that the bacterium was able to produce an active beta-galactosidase in the digestive tract, although it did not multiply during its transit. This...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.2.938-941.2002
更新日期:2002-02-01 00:00:00
abstract::Mathematical models were developed to predict the probability of yeast spoilage of cold-filled ready-to-drink beverages as a function of beverage formulation. A Box-Behnken experimental design included five variables, each at three levels: pH (2.8, 3.3, and 3.8), titratable acidity (0.20, 0.40, and 0.60%), sugar conte...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.4.1901-1906.2002
更新日期:2002-04-01 00:00:00
abstract::We investigated the prevalence of sapoviruses (SaVs) in the Tamagawa River in Japan from April 2003 to March 2004 and performed genetic analysis of the SaV genes identified in river water. A total of 60 river water samples were collected from five sites along the river, and 500 ml was concentrated using the cation-coa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02739-09
更新日期:2010-04-01 00:00:00
abstract::Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This meth...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.2.1692-1695.2006
更新日期:2006-02-01 00:00:00
abstract::Laboratory column studies were conducted at the Utah Water Research Laboratory, Logan, Utah, to evaluate reovirus removal from drinking water supplies by slow-rate sand filtration (SSF). Columns, constructed to simulate a full-scale SSF field operation, were inoculated with reovirus at ca. 1,000-times-greater concentr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.4.818-825.1984
更新日期:1984-10-01 00:00:00
abstract::Diploscapter, a thermotolerant, free-living soil bacterial-feeding nematode commonly found in compost, sewage, and agricultural soil in the United States, was studied to determine its potential role as a vehicle of Salmonella enterica serotype Poona, enterohemorrhagic Escherichia coli O157:H7, and Listeria monocytogen...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.5.2433-2437.2005
更新日期:2005-05-01 00:00:00
abstract::rRNA genetic markers were analyzed in 97 isolates of nodule bacteria from six legume species in Chihuahua, Mexico. The most common genotypes were widely shared across host species and had 16S rRNA sequences identical to those of strains from an eastern North American legume (Amphicarpaea) that are closely related to B...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.4.2044-2048.2002
更新日期:2002-04-01 00:00:00
abstract::The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.9.2742-2747.1990
更新日期:1990-09-01 00:00:00
abstract::A modified glucoamylase gene (glaA) with an extra Thr- and Ser-rich Gp-I domain (T. Semimaru, M. Goto, K. Furukawa, and S. Hayashida, Appl. Environ. Microbiol. 61:2885-2890, 1995) was introduced into a mutant parental host, Aspergillus awamori var. kawachi, in which the original glaA gene had been completely deleted a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.7.2940-2943.1997
更新日期:1997-07-01 00:00:00
abstract::The circulation of human parechoviruses (HPeVs) in the population was studied by environmental surveillance comprising of molecular analyses of sewage samples (n = 89) that were collected from 15 different locations in The Netherlands. Samples were taken from sewage originating from schools (n = 9) or from parts of mu...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01917-13
更新日期:2013-10-01 00:00:00
abstract::Several natural isolates of Escherichia coli O157:H7 have previously been shown to exhibit stationary-phase-dependent variation in their resistance to inactivation by high hydrostatic pressure. In this report we demonstrate that loss of the stationary-phase-inducible sigma factor RpoS resulted in decreased resistance ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.10.4901-4907.2001
更新日期:2001-10-01 00:00:00
abstract::Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is str...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.04028-12
更新日期:2013-05-01 00:00:00
abstract::Cyanase catalyzes the decomposition of cyanate into CO(2) and ammonium, with carbamate as an unstable intermediate. The cyanase of Pseudomonas pseudoalcaligenes CECT5344 was negatively regulated by ammonium and positively regulated by cyanate, cyanide, and some cyanometallic complexes. Cyanase activity was not detecte...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00916-08
更新日期:2008-10-01 00:00:00
abstract::N-acylhomoserine lactones (AHLs) are signaling molecules in many quorum-sensing (QS) systems that regulate interactions between various pathogenic bacteria and their hosts. Quorum quenching by the enzymatic inactivation of AHLs holds great promise in preventing and treating infections, and several such enzymes have be...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00477-10
更新日期:2010-08-01 00:00:00
abstract::Inactivation of infectious viruses during drinking water treatment is usually achieved with free chlorine. Many drinking water utilities in the United States now use monochloramine as a secondary disinfectant to minimize disinfectant by-product formation and biofilm growth. The inactivation of human adenoviruses 2, 40...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01342-09
更新日期:2010-02-01 00:00:00
abstract::An immunolatex reagent was developed from antiserum to Fusobacterium varium, an obligate anaerobe isolated from the colon of the cockroach, Periplaneta americana L. A new technique that enabled the preparation of a highly efficient immunolatex conjugate was used to localize the bacterium with scanning electron microsc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.35.1.166-171.1978
更新日期:1978-01-01 00:00:00
abstract::Strains of Bifidobacterium animalis subsp. lactis are extensively exploited by the food industry as health-promoting bacteria, although the genetic variability of members belonging to this taxon has so far not received much scientific attention. In this article, we describe the complete genetic makeup of the B. animal...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00984-13
更新日期:2013-07-01 00:00:00
abstract::To understand how microbial communities and functional genes respond to arsenic contamination in the rhizosphere of Pteris vittata, five soil samples with different arsenic contamination levels were collected from the rhizosphere of P. vittata and nonrhizosphere areas and investigated by Biolog, geochemical, and funct...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00500-10
更新日期:2010-11-01 00:00:00
abstract::An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.37.2.187-193.1979
更新日期:1979-02-01 00:00:00
abstract::Sourdough lactic acid bacteria were preliminarily screened for proteolytic activity by using a digest of albumin and globulin polypeptides as a substrate. Based on their hydrolysis profile patterns, Lactobacillus alimentarius 15M, Lactobacillus brevis 14G, Lactobacillus sanfranciscensis 7A, and Lactobacillus hilgardii...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.2.623-633.2002
更新日期:2002-02-01 00:00:00
abstract::Resistant and susceptible populations of the diamondback moth (Plutella xylostella) were tested with crystalline, solubilized, and partially and fully activated forms of the Bacillus thuringiensis Cry1Ac delta-endotoxin. Fully activated toxin greatly reduced the resistance ratio (ratio of the 50% lethal concentration ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.9.4372-4373.2001
更新日期:2001-09-01 00:00:00