Effects of temperature abuse on survival of Vibrio vulnificus in oysters.

abstract：:Wood-degrading fungi use a sequence of oxidative and hydrolytic mechanisms to loosen lignocellulose and then release and metabolize embedded sugars. These temporal sequences have recently been mapped at high resolution using directional growth on wood wafers, revealing previously obscured dynamics as fungi progressive...

journal_title：Applied and environmental microbiology

authors： Presley GN,Panisko E,Purvine SO,Schilling JS

更新日期：2018-08-01 00:00:00

abstract：:The production of a stable cDNA copy of an unstable RNA molecule by reverse transcription is a widely used and essential technology for many important applications, such as the construction of gene libraries, production of DNA probes, and analysis of gene expression by reverse transcriptase PCR (RT-PCR). However, the ...

journal_title：Applied and environmental microbiology

authors： Vellore J,Moretz SE,Lampson BC

更新日期：2004-12-01 00:00:00

abstract：:Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

journal_title：Applied and environmental microbiology

authors： Elhanafi D,Dutta V,Kathariou S

更新日期：2010-12-01 00:00:00

abstract：:A thermophilic strain (D2) identified as a Bacillus sp. was isolated from an aerobic digestor of swine waste after several months of operation at 55 degrees C. Aerobic thermophilic batch treatment of swine waste inoculated with strain D2 was studied in a 4-liter fixed-bed reactor. Stabilization of the waste was achiev...

journal_title：Applied and environmental microbiology

authors： Beaudet R,Gagnon C,Bisaillon JG,Ishaque M

更新日期：1990-04-01 00:00:00

abstract：:Norovirus is one of the most common causes of acute viral gastroenteritis. The virus is spread via the fecal-oral route, most commonly from infected food and water, but several outbreaks have originated from contamination of surfaces with infectious virus. In this study, a close surrogate of human norovirus causing ga...

journal_title：Applied and environmental microbiology

authors： Warnes SL,Summersgill EN,Keevil CW

更新日期：2015-02-01 00:00:00

abstract：:Fecal droppings of migratory Canada geese, Branta canadensis, collected from nine sites near the Chesapeake Bay (Maryland), were examined for the presence of Cryptosporidium parvum and Giardia spp. Cryptosporidium sp. oocysts were found in feces at seven of nine sites, and Giardia cysts were found at all nine sites. T...

journal_title：Applied and environmental microbiology

authors： Graczyk TK,Fayer R,Trout JM,Lewis EJ,Farley CA,Sulaiman I,Lal AA

更新日期：1998-07-01 00:00:00

abstract：:Pili (fimbriae) were observed on cells of each of the five strains of Bradyrhizobium japonicum and the one strain of Rhizobium trifolii examined. Pili on B. japonicum were about 4 nm in diameter and polarly expressed. Piliated cells were estimated by transmission electron microscopy and hydrophobic attachment to polys...

journal_title：Applied and environmental microbiology

authors： Vesper SJ,Bauer WD

更新日期：1986-07-01 00:00:00

abstract：:Lake Fryxell is a perennially ice-covered lake in the McMurdo Dry Valleys, Antarctica, with a sharp oxycline in a water column that is density stabilized by a gradient in salt concentration. Dissolved oxygen falls from 20 mg liter(-1) to undetectable over one vertical meter from 8.9- to 9.9-m depth. We provide the fir...

journal_title：Applied and environmental microbiology

authors： Jungblut AD,Hawes I,Mackey TJ,Krusor M,Doran PT,Sumner DY,Eisen JA,Hillman C,Goroncy AK

更新日期：2015-11-13 00:00:00

abstract：:Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and catalytic efficiency of ...

journal_title：Applied and environmental microbiology

authors： Duan X,Wu J

更新日期：2015-03-01 00:00:00

abstract：:Morphological changes of Vibrio parahaemolyticus from rods to spheres took place after a culture was subjected to starvation at a wide range of temperatures. Scanning electron micrographs revealed that starved spherical cells gradually developed a rippled cell surface with blebs and an extracellular filamentous substa...

journal_title：Applied and environmental microbiology

authors： Jiang X,Chai TJ

更新日期：1996-04-01 00:00:00

abstract：:We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. Species-specific quantification was achieved by targeting the chromosomal monocopy gene choE, universally conserved in R. equi. The choE Q-PCR included an ...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Lewis DA,Ocampo-Sosa AA,Fogarty U,Makrai L,Navas J,Scortti M,Hernández M,Vázquez-Boland JA

更新日期：2006-06-01 00:00:00

abstract：:The role of the storage lipid poly-beta-hydroxybutyrate (PHB) in trichloroethylene transformation by methanotrophic mixed cultures was investigated. Naphthalene oxidation rates were used to assay for soluble methane monooxygenase activity. The PHB content of methanotrophic cells grown in reactors varied diurnally as w...

journal_title：Applied and environmental microbiology

authors： Henrysson T,McCarty PL

更新日期：1993-05-01 00:00:00

abstract：:The temperate bacteriophage phiLC3, isolated from Lactococcus lactis subsp. cremoris, has an isometric head and a flexible tail containing 1 major protein and 8 minor proteins. Infection of a permissive L. lactis host strain yields a burst of about 50 phages per infected cell with a latent period of 60 min. A detailed...

journal_title：Applied and environmental microbiology

authors： Lillehaug D,Lindqvist B,Birkeland NK

更新日期：1991-11-01 00:00:00

abstract：:Thiobacillus ferrooxidans is an acidophilic organism important to metal leaching of low-grade ores. The aforementioned importance is related to the ability of the bacterium to oxidize reduced iron and sulfur, principally found in nature as pyrite (FeS2). The present study dealt with sulfide oxidation at low pH values ...

journal_title：Applied and environmental microbiology

authors： Tano T,Lundgren D

更新日期：1978-06-01 00:00:00

abstract：:Some Listeria monocytogenes internalins are recognized as contributing to invasion of mammalian tissue culture cells. While PrfA is well established as a positive regulator of L. monocytogenes virulence gene expression, the stress-responsive sigma(B) has been recognized only recently as contributing to expression of v...

journal_title：Applied and environmental microbiology

authors： McGann P,Wiedmann M,Boor KJ

更新日期：2007-05-01 00:00:00

abstract：:H(2) generated from renewable resources holds promise as an environmentally innocuous fuel that releases only energy and water when consumed. In biotechnology, photoautotrophic oxygenic diazotrophs could produce H(2) from water and sunlight using the cells' endogenous nitrogenases. However, nitrogenases have low turno...

journal_title：Applied and environmental microbiology

authors： Gärtner K,Lechno-Yossef S,Cornish AJ,Wolk CP,Hegg EL

更新日期：2012-12-01 00:00:00

abstract：:Eight strains of the entomopathogen Bacillus popilliae were analyzed by pulsed-field gel electrophoresis, and genomic size estimates of ;sim2,600 to 3,500 kb were obtained. The type strain, ATCC 14706, had a genomic size of 3,395 kb. For the six New Zealand isolates, the degree of similarity in the pulsed-field gel el...

journal_title：Applied and environmental microbiology

authors： Macdonald R,Kalmakoff J

更新日期：1995-06-01 00:00:00

abstract：:Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...

journal_title：Applied and environmental microbiology

authors： Uchiyama H,Nakajima T,Yagi O,Nakahara T

更新日期：1992-09-01 00:00:00

abstract：:The pathways for degradation of aromatic hydrocarbons are constantly modified by a variety of genetic mechanisms. Genetic studies carried out with Pseudomonas stutzeri OX1 suggested that the tou operon coding for toluene o-xylene monooxygenase (ToMO) was recently recruited into a preexisting pathway that already posse...

journal_title：Applied and environmental microbiology

authors： Cafaro V,Notomista E,Capasso P,Di Donato A

更新日期：2005-08-01 00:00:00

abstract：:Culture-independent (PCR with Crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of Crenarchaeota in terrestrial hot springs of the Kamchatka Peninsula and the Lake Baikal region (Russia) and of Iceland. Among the phy...

journal_title：Applied and environmental microbiology

authors： Perevalova AA,Kolganova TV,Birkeland NK,Schleper C,Bonch-Osmolovskaya EA,Lebedinsky AV

更新日期：2008-12-01 00:00:00

abstract：:UNL-1, a lytic virus of Pseudomonas aeruginosa, was observed to express a novel inducible DNA damage reactivation activity in UV-A-irradiated P. aeruginosa host cells. The expression of bacteriophage reactivation was quantified in hosts exposed to either UV-C or UV-A radiation. While reactivation of UV-C-damaged UNL-1...

journal_title：Applied and environmental microbiology

authors： Shaffer JJ,Jacobsen LM,Schrader JO,Lee KW,Martin EL,Kokjohn TA

更新日期：1999-06-01 00:00:00

abstract：:Bacteroidales are attractive as water quality indicators because of their potential to discern sources of fecal pollution, and it is presumed that these bacteria do not multiply outside their host organisms. The persistence of a fecal Bacteroidales marker was monitored over 14 days in river water microcosms that varie...

journal_title：Applied and environmental microbiology

authors： Schulz CJ,Childers GW

更新日期：2011-04-01 00:00:00

abstract：:Lantibiotics are ribosomally synthesized antimicrobial peptides with substantial posttranslational modifications. They are characterized by the unique amino acids lanthionine and methyllanthionine, which are introduced by dehydration of Ser/Thr residues and linkage of the resulting dehydrated amino acids with Cys resi...

journal_title：Applied and environmental microbiology

authors： Basi-Chipalu S,Dischinger J,Josten M,Szekat C,Zweynert A,Sahl HG,Bierbaum G

更新日期：2015-05-15 00:00:00

abstract：:The type III secretion system (T3SS) is a specialized secretion apparatus that is commonly used by many plant and animal pathogenic bacteria to deliver proteins, termed effectors, to the interior of the host cells. These effectors suppress host defenses and interfere with signal transduction pathways to promote infect...

journal_title：Applied and environmental microbiology

authors： Jiménez-Guerrero I,Pérez-Montaño F,Medina C,Ollero FJ,López-Baena FJ

更新日期：2017-02-15 00:00:00

abstract：:The changes in motility, chemotactic responsiveness, and flagellation of Rhizobium meliloti RMB7201, L5-30, and JJ1c10 were analyzed after transfer of the bacteria to buffer with no available C, N, or phosphate. Cells of these three strains remained viable for weeks after transfer to starvation buffer (SB) but lost al...

journal_title：Applied and environmental microbiology

authors： Wei X,Bauer WD

更新日期：1998-05-01 00:00:00

abstract：:Washed cell suspensions of the facultative methylotroph strain IMB-1 grown on methyl bromide (MeBr) were able to consume methyl chloride (MeCl) and methyl iodide (MeI) as well as MeBr. Consumption of >100 microM MeBr by cells grown on glucose, acetate, or monomethylamine required induction. Induction was inhibited by ...

journal_title：Applied and environmental microbiology

authors： Schaefer JK,Oremland RS

更新日期：1999-11-01 00:00:00

abstract：:In raw sludges and in mesophilically and thermophilically digested anaerobic sludges, large variations in numbers of viruses occurred over narrow ranges of numbers of fecal coliforms, total coliforms, and fecal streptococci, demonstrating that the bacteria were poor quantitative reflectors of the numbers of the viruse...

journal_title：Applied and environmental microbiology

authors： Berg G,Berman D

更新日期：1980-02-01 00:00:00

abstract：:Copper appears to be influencing the distribution and abundance of phytoplankton in marine environments, and cyanobacteria are thought to be the most sensitive of the phytoplankton groups to copper toxicity. By using growth assays of phylogenetically divergent clades, we found that coastal strains of marine Synechococ...

journal_title：Applied and environmental microbiology

authors： Stuart RK,Dupont CL,Johnson DA,Paulsen IT,Palenik B

更新日期：2009-08-01 00:00:00

abstract：:Forty-nine isolates of Butyrivibrio fibrisolvens and a single isolate of Butyrivibrio crossotus were screened for the production of inhibitors by a deferred plating procedure. Twenty-five isolates produced factors which, to various degrees, inhibited the growth of the other Butyrivibrio isolates. None of the inhibitor...

journal_title：Applied and environmental microbiology

authors： Kalmokoff ML,Teather RM

更新日期：1997-02-01 00:00:00

abstract：:Clostridium botulinum 62A and ATCC 25763 spores required heat activation for maximum colony formation when plated on reinforced clostridial agar (BBL Microbiology Systems) but not when plated on botulinum assay medium. Spores from strains B-aphis and 53B did not exhibit heat activation when plated on either medium. ...

journal_title：Applied and environmental microbiology

authors： Montville TJ

更新日期：1981-10-01 00:00:00