Survival of Vibrio parahaemolyticus at low temperatures under starvation conditions and subsequent resuscitation of viable, nonculturable cells.

abstract：:An l-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of d,l-amino acid amide racemates, was purified to homogeneity. The highly l-enantioselective enzyme resembled thiol reagent-sensitive alkaline serine proteinases and was strongly activated by divalent cations. It possessed a h...

journal_title：Applied and environmental microbiology

authors： Hermes HF,Sonke T,Peters PJ,van Balken JA,Kamphuis J,Dijkhuizen L,Meijer EM

更新日期：1993-12-01 00:00:00

abstract：:A novel Bacillus thuringiensis crystal protein with a silent activity against the Colorado potato beetle is described. The crystal proteins are produced as bipyramidal crystals. These crystals contain a protein of 129 kDa with a trypsin-resistant core fragment of 72 kDa. Neither a spore-crystal mixture nor in vitro-so...

journal_title：Applied and environmental microbiology

authors： Lambert B,Höfte H,Annys K,Jansens S,Soetaert P,Peferoen M

更新日期：1992-08-01 00:00:00

abstract：:The Association of Official Analytical Chemists (AOAC) test for assessing the tuberculocidal activity of disinfectants has been shown to be variable. A modified AOAC test, which substituted Middlebrook 7H9 broth as the primary subculture medium and used neutralization by dilution, was compared with the standard AOAC m...

journal_title：Applied and environmental microbiology

authors： Cole EC,Rutala WA,Nessen L,Wannamaker NS,Weber DJ

更新日期：1990-06-01 00:00:00

abstract：:Parental strains and asporogenous mutants of Bacillus thuringiensis subspp. kurstaki and aizawai produced high yields of delta-endotoxin on M medium, which contained 330 mug of potassium per ml, but not on ST and ST-a media, each of which contained only 11 mug of potassium per ml. On ST and ST-a media, refractile gran...

journal_title：Applied and environmental microbiology

authors： Wakisaka Y,Masaki E,Nishimoto Y

更新日期：1982-06-01 00:00:00

abstract：:The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was clos...

journal_title：Applied and environmental microbiology

authors： Dedysh SN,Pankratov TA,Belova SE,Kulichevskaya IS,Liesack W

更新日期：2006-03-01 00:00:00

abstract：:In this work, we report the expression and secretion of the leaderless two-peptide (EntL50A and EntL50B) bacteriocin enterocin L50 from Enterococcus faecium L50 by the methylotrophic yeast Pichia pastoris X-33. The bacteriocin structural genes entL50A and entL50B were fused to the Saccharomyces cerevisiae gene region ...

journal_title：Applied and environmental microbiology

authors： Basanta A,Gómez-Sala B,Sánchez J,Diep DB,Herranz C,Hernández PE,Cintas LM

更新日期：2010-05-01 00:00:00

abstract：:Strain DCB-1 is an obligately anaerobic bacterium which carries out the reductive dehalogenation of halobenzoates and was previously known to grow only on pyruvate plus 20% ruminal fluid. When various electron acceptors were supplied, thiosulfate and sulfite were found to stimulate growth. Sulfide was produced from th...

journal_title：Applied and environmental microbiology

authors： Stevens TO,Linkfield TG,Tiedje JM

更新日期：1988-12-01 00:00:00

abstract：:Two gram-positive, strictly anoxic, coccoid- to rod-shaped strains of bacteria, Clostridium coccoides 1410 and C. coccoides 3110, were isolated from human feces on the typical homoacetogenic substrates formate plus H2 plus CO2 (strain 1410) and vanillate plus H2 plus CO2 (strain 3110) in the presence of 2-bromoethanes...

journal_title：Applied and environmental microbiology

authors： Kamlage B,Gruhl B,Blaut M

更新日期：1997-05-01 00:00:00

abstract：:Rsan-ver, a strain of Pseudomonas aeruginosa isolated at this department, was used for the development of a continuous process for biosurfactant production. The active compounds were identified as rhamnolipids. A final medium for production was designed in continuous culture by means of medium shifts, since the format...

journal_title：Applied and environmental microbiology

authors： Guerra-Santos L,Käppeli O,Fiechter A

更新日期：1984-08-01 00:00:00

abstract：:A survey was made to detect the sensitivity of 12 yeast genera to T-2 toxin. Seventy-five yeasts isolated from various sources were tested for their susceptibility to T-2 toxin. The MIC of T-2 for these yeasts varied from 1.0 to greater than 8.0 micrograms/ml. Of the yeasts studied, Kluyveromyces fragilis showed the g...

journal_title：Applied and environmental microbiology

authors： Sukroongreung S,Schappert KT,Khachatourians GG

更新日期：1984-08-01 00:00:00

abstract：:After treatment of a dispersed suspension of echovirus with HOCl, much of the lost plaque titer was restored if the treated virus was induced to aggregate by adjustment of the suspending medium to pH 4.5. This did not appear to be a repair of individual virions but rather a special kind of multiplicity-related increas...

journal_title：Applied and environmental microbiology

authors： Young DC,Sharp DG

更新日期：1979-04-01 00:00:00

abstract：:Submicronic particles released from fungal cultures have been suggested to be additional sources of personal exposure in mold-contaminated buildings. In vitro generation of these particles has been studied with particle counters, eventually supplemented by autofluorescence, that recognize fragments by size and discrim...

journal_title：Applied and environmental microbiology

authors： Afanou KA,Straumfors A,Skogstad A,Nilsen T,Synnes O,Skaar I,Hjeljord L,Tronsmo A,Green BJ,Eduard W

更新日期：2014-11-01 00:00:00

abstract：:Relative to those of unexposed cultures, the transcript levels of the four CprA-type reductive dehalogenase genes (cprA2, cprA3, cprA4, and cprA5) in Desulfitobacterium hafniense PCP-1 were measured in cultures exposed to chlorophenols. In 2,4,6-trichlorophenol-amended cultures, cprA2 and cprA3 were upregulated, as wa...

journal_title：Applied and environmental microbiology

authors： Bisaillon A,Beaudet R,Lépine F,Villemur R

更新日期：2011-09-01 00:00:00

abstract：:Bacterium T-52, cultured on ethylene glycol, readily oxidized glycolate and glyoxylate and exhibited elevated activities of ethylene glycol dehydrogenase and glycolate oxidase. Labeled glyoxylate was identified in reaction mixtures containing [C]-ethylene glycol, but no glycolate was detected. The most likely pathway ...

journal_title：Applied and environmental microbiology

authors： Caskey WH,Taber WA

更新日期：1981-07-01 00:00:00

abstract：:Pseudomonas aureofaciens PGS12 produces three phenazine antibiotics, in addition to siderophores, hydrogen cyanide, pyrrolnitrin, and indoleacetic acid. Tn5-259.7 transposon mutagenesis was carried out to identify and clone a chromosomal locus involved in phenazine biosynthesis. Three classes of mutants were obtained:...

journal_title：Applied and environmental microbiology

authors： Georgakopoulos DG,Hendson M,Panopoulos NJ,Schroth MN

更新日期：1994-08-01 00:00:00

abstract：:Ten laboratories participated in an interlaboratory comparison of determination of bioleaching rates of a pyrite reference material. A standardized procedure and a single strain of Thiobacillus ferrooxidans were used in this study. The mean rate of bioleaching of the pyrite reference material was 12.4 mg of Fe per lit...

journal_title：Applied and environmental microbiology

authors： Olson GJ

更新日期：1991-03-01 00:00:00

abstract：:Bacillus megaterium P1, a bacterial strain capable of hydrolyzing chitosan, was isolated from soil samples. Chitosan-degrading activity was induced by chitosan but not by its constituent d-glucosamine. Extracellular secretion of chitosanase reached levels corresponding to 1 U/ml under optimal conditions. Three chitosa...

journal_title：Applied and environmental microbiology

authors： Pelletier A,Sygusch J

更新日期：1990-04-01 00:00:00

abstract：:Two Aeromonas hydrophila bacteriophages, Aeh1 and Aeh2, were isolated from sewage. Both phages showed binal symmetry. The dimensions of A. hydrophila phages Aeh1 and Aeh2 differed from those of the other Aeromonas phages. Also, phage Aeh2 was the largest Aeromonas phage studied to date. Phage Aeh1 formed small, clear ...

journal_title：Applied and environmental microbiology

authors： Chow MS,Rouf MA

更新日期：1983-05-01 00:00:00

abstract：:We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays. ...

journal_title：Applied and environmental microbiology

authors： Alvarez-Ortega C,Harwood CS

更新日期：2007-12-01 00:00:00

abstract：:Genetic competence in Streptococcus mutans is a transient state that is regulated in response to multiple environmental inputs. These include extracellular pH and the concentrations of two secreted peptides, designated CSP (competence-stimulating peptide) and XIP (comX-inducing peptide). The role of environmental cues...

journal_title：Applied and environmental microbiology

authors： Son M,Ghoreishi D,Ahn SJ,Burne RA,Hagen SJ

更新日期：2015-08-15 00:00:00

abstract：:Infectious hematopoietic necrosis virus (IHNV) is a pathogen of young salmon and trout. Viral epizootics among these fish in private and public rearing facilities have been a problem in the northwestern United States from California to Alaska, and an IHNV vaccine has been sought by the aquaculture experts. Since an IH...

journal_title：Applied and environmental microbiology

authors： Engelking HM,Harry JB,Leong JA

更新日期：1991-05-01 00:00:00

abstract：:Cytophaga hutchinsonii is a widely distributed cellulolytic bacterium in the phylum Bacteroidetes. It can digest crystalline cellulose rapidly without free cellulases or cellulosomes. The mechanism of its cellulose utilization remains a mystery. We developed an efficient method based on a linear DNA double-crossover a...

journal_title：Applied and environmental microbiology

authors： Wang Y,Wang Z,Cao J,Guan Z,Lu X

更新日期：2014-10-01 00:00:00

abstract：:Over 200 bacterial strains were selected for anaerobic growth at 50 degrees C and extracellular polysaccharide production in a sucrose-mineral salts medium with NaNO(3) and up to 10% NaCl. The predominant cell type was an encapsulated gram-positive, motile, facultative sporeforming rod similar to Bacillus species. Str...

journal_title：Applied and environmental microbiology

authors： Pfiffner SM,McInerney MJ,Jenneman GE,Knapp RM

更新日期：1986-06-01 00:00:00

abstract：:Fusarium verticillioides (Gibberella fujikuroi mating population A [MP-A]) is a widespread pathogen on maize and is well-known for producing fumonisins, mycotoxins that cause severe disease in animals and humans. The species is a member of the Gibberella fujikuroi species complex, which consists of at least 11 differe...

journal_title：Applied and environmental microbiology

authors： Bömke C,Rojas MC,Hedden P,Tudzynski B

更新日期：2008-12-01 00:00:00

abstract：:A system of tangential-flow filtration was evaluated for use in the detection of Giardia cysts in drinking water. This method was more sensitive in recovering cysts than a frequently used wound-orlon system of through-filtration. ...

journal_title：Applied and environmental microbiology

authors： Isaac-Renton JL,Fung CP,Lochan A

更新日期：1986-08-01 00:00:00

abstract：:Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

journal_title：Applied and environmental microbiology

authors： Ramírez-Reinat EL,Garcia-Pichel F

更新日期：2012-01-01 00:00:00

abstract：:A number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. No single one of the indicators tested to date has been able to determine the source of fecal pollution in water. However, the combined use of different indicators has been demonstrat...

journal_title：Applied and environmental microbiology

authors： Ballesté E,Bonjoch X,Belanche LA,Blanch AR

更新日期：2010-03-01 00:00:00

abstract：:Eight concentration and purification methods were evaluated to determine percentages of recovery of Cryptosporidium parvum oocysts from calf feces. The NaCl flotation method generally resulted in the highest percentages of recovery. Based on the percentages of recovery, the amounts of fecal debris in the final oocyst ...

journal_title：Applied and environmental microbiology

authors： Kuczynska E,Shelton DR

更新日期：1999-07-01 00:00:00

abstract：:The arginine deiminase system in a variety of streptococci and in Pseudomonas aeruginosa was found to be unusually acid tolerant in that arginolysis occurred at pH values well below the minima for growth and glycolysis. The acid tolerance of the system allowed bacteria to survive potentially lethal acidification throu...

journal_title：Applied and environmental microbiology

authors： Marquis RE,Bender GR,Murray DR,Wong A

更新日期：1987-01-01 00:00:00

abstract：:The aim of this work was to identify genes in Lactococcus lactis subsp. lactis IL1403 and Lactococcus lactis subsp. cremoris Wg2 important for adsorption of the 936-species phages bIL170 and phi 645, respectively. Random insertional mutagenesis of the two L. lactis strains was carried out with the vector pGh9:ISS1, an...

journal_title：Applied and environmental microbiology

authors： Dupont K,Janzen T,Vogensen FK,Josephsen J,Stuer-Lauridsen B

更新日期：2004-10-01 00:00:00