Physiological characterization of strain DCB-1, a unique dehalogenating sulfidogenic bacterium.

Abstract:

:Strain DCB-1 is an obligately anaerobic bacterium which carries out the reductive dehalogenation of halobenzoates and was previously known to grow only on pyruvate plus 20% ruminal fluid. When various electron acceptors were supplied, thiosulfate and sulfite were found to stimulate growth. Sulfide was produced from thiosulfate. Cytochrome c and desulfoviridin were detected. The mol% G+C was 49 (at the thermal denaturation temperature). Of 55 carbon sources tested, only pyruvate supported growth as the sole carbon source in mineral medium. Lactate, acetate, L- and D-malate, glycerol, and L- and D-arabinose stimulated growth when supplemented with 10% ruminal fluid and 20 mM thiosulfate. In mineral medium, pyruvate was converted to acetate and lactate, with small amounts of succinate and fumarate accumulating transiently. During growth with thiosulfate, all of these products accumulated transiently. Addition of excess hydrogen to pyruvate-grown cultures resulted in diversion of carbon to formate, lactate, and butyrate, which caused a decrease in cell yield. We conclude that strain DCB-1 is a new type of sulfidogenic bacterium.

journal_name

Appl Environ Microbiol

authors

Stevens TO,Linkfield TG,Tiedje JM

doi

10.1128/AEM.54.12.2938-2943.1988

subject

Has Abstract

pub_date

1988-12-01 00:00:00

pages

2938-43

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

54

pub_type

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