Methodology of protistan discovery: from rRNA detection to quality scanning electron microscope images.

abstract：:The transmission of Escherichia coli O157:H7 from manure-contaminated soil and irrigation water to lettuce plants was demonstrated using laser scanning confocal microscopy, epifluorescence microscopy, and recovery of viable cells from the inner tissues of plants. E. coli O157:H7 migrated to internal locations in plant...

journal_title：Applied and environmental microbiology

authors： Solomon EB,Yaron S,Matthews KR

更新日期：2002-01-01 00:00:00

abstract：:Gonometa podocarpi is an important pest of several species of pine in East Africa, and large numbers of trees in plantations in Kenya were partially or completely defoliated by the larval stage of this insect. After the infestation in the Mt. Elgon region, large numbers of dead and moribund larvae were found on the gr...

journal_title：Applied and environmental microbiology

authors： Moore NF,Armstrong T,Green B,Brown D,Hibbin J,Kelly DC,Tinsley TW,Aloo TC

更新日期：1981-08-01 00:00:00

abstract：:Studies of time-dependent drug and environmental effects on single, live bacterial cells would benefit significantly from a permeable, nonperturbative, long-lived fluorescent stain specific to the nucleoids (chromosomal DNA). The ideal stain would not affect cell growth rate or nucleoid morphology and dynamics, even d...

journal_title：Applied and environmental microbiology

authors： Bakshi S,Choi H,Rangarajan N,Barns KJ,Bratton BP,Weisshaar JC

更新日期：2014-08-01 00:00:00

abstract：:Glycerol fermentation by Enterobacter agglomerans revealed that both growth and 1,3-propanediol production ceased after consumption of about 430 mM glycerol, irrespective of the initial glycerol content. This phenomenon was assigned to the production of 3-hydroxypropionaldehyde, which was identified by proton nuclear ...

journal_title：Applied and environmental microbiology

authors： Barbirato F,Grivet JP,Soucaille P,Bories A

更新日期：1996-04-01 00:00:00

abstract：:Bacillus thuringiensis produces large amounts of various pesticidal proteins during the stationary phase. In order to achieve a high yield and form crystals, some pesticidal proteins require the presence of other proteins. Helper protein P20 is required for efficient production of both the Cyt1A and Cry11A crystal pro...

journal_title：Applied and environmental microbiology

authors： Shao Z,Liu Z,Yu Z

更新日期：2001-12-01 00:00:00

abstract：:Kidneys showing renal changes characteristic for mycotoxic porcine nephropathy were collected during the period 1 April 1982 to 31 March 1983 from 225,000 swine processed in a large slaughterhouse in the district of Poznań, Poland. Of 113 kidneys suspected of mycotoxic porcine nephropathy, 27 exhibited ochratoxin A le...

journal_title：Applied and environmental microbiology

authors： Goliński P,Hult K,Grabarkiewicz-Szczesna J,Chełkowski J,Kneblewski P,Szebiotko K

更新日期：1984-06-01 00:00:00

abstract：:The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when ...

journal_title：Applied and environmental microbiology

authors： Fujito BT,Lytle CD

更新日期：1996-09-01 00:00:00

abstract：:We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and...

journal_title：Applied and environmental microbiology

authors： Satokari RM,Vaughan EE,Akkermans AD,Saarela M,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:The consumption of acetate in soils taken from a nutrient gradient in the northern Florida Everglades was studied by stable isotope probing. Bacterial and archaeal 16S rRNA gene clone libraries from eutrophic and oligotrophic soil microcosms strongly suggest that a significant amount of acetate is consumed by syntroph...

journal_title：Applied and environmental microbiology

authors： Chauhan A,Ogram A

更新日期：2006-10-01 00:00:00

abstract：:Erysipelothrix rhusiopathiae causes swine erysipelas, an important infectious disease in the swine industry. In Japan, the incidence of acute swine erysipelas due to E. rhusiopathiae serovar 1a has recently increased markedly. To study the genetic relatedness of the strains from the recent cases, we analyzed 34 E. rhu...

journal_title：Applied and environmental microbiology

authors： Ogawa Y,Shiraiwa K,Ogura Y,Ooka T,Nishikawa S,Eguchi M,Hayashi T,Shimoji Y

更新日期：2017-05-17 00:00:00

abstract：:The impact of host nutrition on symbiont regulation in the pea aphid Acyrthosiphon pisum was investigated. The population density of the obligate symbiont Buchnera aphidicola positively correlated with dietary nitrogen levels. In contrast, the population density of the facultative symbiont Serratia symbiotica increase...

journal_title：Applied and environmental microbiology

authors： Wilkinson TL,Koga R,Fukatsu T

更新日期：2007-02-01 00:00:00

abstract：:A stable bacterial community absorbed and transformed the herbicide metolachlor [2-chloro-N-(2-ethyl- 6-methylphenyl)-N-(2-methoxy-1-methylethyl)-acetamide] from a liquid medium. About 80% of the added ring-[U-C]metolachlor (50 mug/ml) disappeared from the medium and accumulated inside the cells. The ratio of cellular...

journal_title：Applied and environmental microbiology

authors： Liu SY,Zheng Z,Zhang R,Bollag JM

更新日期：1989-03-01 00:00:00

abstract：:We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the ass...

journal_title：Applied and environmental microbiology

authors： Sharma NK,Rees CE,Dodd CE

更新日期：2000-04-01 00:00:00

abstract：:Four soil temperature and moisture treatment regimens were imposed on Florunner peanuts 94 days after planting in experimental plots in 1980. At harvest (145 days after planting), the incidence of the Aspergillus flavus group and the aflatoxin concentration were greatest in damaged kernels. Extensive colonization of s...

journal_title：Applied and environmental microbiology

authors： Hill RA,Blankenship PD,Cole RJ,Sanders TH

更新日期：1983-02-01 00:00:00

abstract：:To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) ...

journal_title：Applied and environmental microbiology

authors： Villarreal DM,Phillips CL,Kelley AM,Villarreal S,Villaloboz A,Hernandez P,Olson JS,Henderson DP

更新日期：2008-09-01 00:00:00

abstract：:Salmonella spp. are remarkably adaptable pathogens, and this adaptability allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amid the native microbiota remain poorly understood. Here, we aimed to uncover the mechanisms that enable ...

journal_title：Applied and environmental microbiology

authors： George AS,Cox CE,Desai P,Porwollik S,Chu W,de Moraes MH,McClelland M,Brandl MT,Teplitski M

更新日期：2018-02-14 00:00:00

abstract：:Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

journal_title：Applied and environmental microbiology

authors： Rapala J,Sivonen K,Lyra C,Niemelä SI

更新日期：1997-06-01 00:00:00

abstract：:The respiration of three phenotypes of Histoplasma capsulatum, the causal agent of histoplasmosis in humans, was markedly reduced by low concentrations of montmorillonite but was reduced less by even higher concentrations of kaolinite or attapulgite (palygorskite). The reduction in respiration followed a pattern that ...

journal_title：Applied and environmental microbiology

authors： Lavie S,Stotzky G

更新日期：1986-01-01 00:00:00

abstract：:β-Aminopeptidases have the unique capability to hydrolyze N-terminal β-amino acids, with varied preferences for the nature of β-amino acid side chains. This unique capability makes them useful as biocatalysts for synthesis of β-peptides and to kinetically resolve β-peptides and amides for the production of enantiopure...

journal_title：Applied and environmental microbiology

authors： John-White M,Gardiner J,Johanesen P,Lyras D,Dumsday G

更新日期：2019-07-18 00:00:00

abstract：:Most species of the diazotrophic Proteobacteria Azoarcus spp. occur in association with grass roots, while A. tolulyticus and A. evansii are soil bacteria not associated with a plant host. To facilitate species identification and strain comparison, we developed a protocol for PCR-generated genomic fingerprints, using ...

journal_title：Applied and environmental microbiology

authors： Hurek T,Wagner B,Reinhold-Hurek B

更新日期：1997-11-01 00:00:00

abstract：:Strain DCB-1 is an obligately anaerobic bacterium which carries out the reductive dehalogenation of halobenzoates and was previously known to grow only on pyruvate plus 20% ruminal fluid. When various electron acceptors were supplied, thiosulfate and sulfite were found to stimulate growth. Sulfide was produced from th...

journal_title：Applied and environmental microbiology

authors： Stevens TO,Linkfield TG,Tiedje JM

更新日期：1988-12-01 00:00:00

abstract：:We detected and identified genotypes of human-pathogenic microsporidia in fecal samples from 51 asymptomatic captive-bred pet parrots in South Korea. Microsporidia were identified in 8 samples (15.7%); 7 parrots tested positive for Encephalitozoon hellem, and 1 parrot tested positive for both E. hellem and Encephalito...

journal_title：Applied and environmental microbiology

authors： Lee SY,Lee SS,Lyoo YS,Park HM

更新日期：2011-12-01 00:00:00

abstract：:Flow cytometry was used to enumerate and characterize bacteria from a sand column microcosm simulating aquifer conditions. Pure cultures of a species of Bacillus isolated from subsurface sediments or Bacillus megaterium were first evaluated to identify these organisms' characteristic histograms. Counting was then carr...

journal_title：Applied and environmental microbiology

authors： Deleo PC,Baveye P

更新日期：1996-12-01 00:00:00

abstract：:Many people in Japan often detect an unpleasant odor generated from laundry that is hung to dry indoors or when using their already-dried laundry. Such an odor is often described as a "wet-and-dirty-dustcloth-like malodor" or an "acidic or sweaty odor." In this study, we isolated the major microorganisms associated wi...

journal_title：Applied and environmental microbiology

authors： Kubota H,Mitani A,Niwano Y,Takeuchi K,Tanaka A,Yamaguchi N,Kawamura Y,Hitomi J

更新日期：2012-05-01 00:00:00

abstract：:Despite a wealth of studies examining the toxicity of engineered nanomaterials, current knowledge on their cytotoxic mechanisms (particularly from a physical perspective) remains limited. In this work, we imaged and quantitatively characterized the biomechanical (hardness and elasticity), adhesive, and surface electri...

journal_title：Applied and environmental microbiology

authors： Zhang W,Hughes J,Chen Y

更新日期：2012-06-01 00:00:00

abstract：:Genomic probes were used to investigate hepatitis A virus (HAV) and enterovirus RNAs in two types of shellfish from natural beds (Atlantic coast, France). After elution concentration, nucleic acid extracted by proteinase K and purified by phenol-chloroform and ethanol precipitation was assayed by dot blot hybridizatio...

journal_title：Applied and environmental microbiology

authors： Le Guyader F,Apaire-Marchais V,Brillet J,Billaudel S

更新日期：1993-11-01 00:00:00

abstract：:Regulation of the expression of the two major chitinase genes, ech42 (encoding the CHIT42 endochitinase) and nag1 (encoding the CHIT73 N-acetyl-beta-D-glucosaminidase), of the chitinolytic system of the mycoparasitic biocontrol fungus Trichoderma atroviride (= Trichoderma harzianum P1) was investigated by using a repo...

journal_title：Applied and environmental microbiology

authors： Mach RL,Peterbauer CK,Payer K,Jaksits S,Woo SL,Zeilinger S,Kullnig CM,Lorito M,Kubicek CP

更新日期：1999-05-01 00:00:00

abstract：:We previously reported the isolation of a 21.5-kb genomic DNA fragment from Pseudomonas sp. strain ADP, which contains the atzA gene, encoding the first metabolic step for the degradation of the herbicide atrazine (M. de Souza, L. P. Wackett, K. L. Boundy-Mills, R. T. Mandelbaum, and M. J. Sadowsky, Appl. Environ. Mic...

journal_title：Applied and environmental microbiology

authors： Boundy-Mills KL,de Souza ML,Mandelbaum RT,Wackett LP,Sadowsky MJ

更新日期：1997-03-01 00:00:00

abstract：:Bacterial aggregates from a chemolithoautotrophic, nitrifying fluidized bed reactor were investigated with microsensors and rRNA-based molecular techniques. The microprofiles of O2, NH4+, NO2-, and NO3- demonstrated the occurrence of complete nitrification in the outer 125 microgram of the aggregates. The ammonia oxid...

journal_title：Applied and environmental microbiology

authors： Schramm A,De Beer D,Wagner M,Amann R

更新日期：1998-09-01 00:00:00

abstract：:The utilization by Alcaligenes faecalis of electrodes as the electron donor for denitrification was investigated in this study. The denitrification rate of A. faecalis with a poised potential was greatly enhanced compared with that of the controls without poised potentials. For nitrate reduction, although A. faecalis ...

journal_title：Applied and environmental microbiology

authors： Wang X,Yu P,Zeng C,Ding H,Li Y,Wang C,Lu A

更新日期：2015-08-15 00:00:00