Identification and activities in situ of Nitrosospira and Nitrospira spp. as dominant populations in a nitrifying fluidized bed reactor.

abstract：:Gordonia polyisoprenivorans strain VH2, a potent rubber-degrading actinomycete, harbors two latex clearing proteins (Lcps), which are known to be essential for the microbial degradation of rubber. However, biochemical information on the exact role of this protein in the degradation of polyisoprene was lacking. In this...

journal_title：Applied and environmental microbiology

authors： Hiessl S,Böse D,Oetermann S,Eggers J,Pietruszka J,Steinbüchel A

更新日期：2014-09-01 00:00:00

abstract：:Large surface-to-volume ratios provide optimal nutrient uptake conditions for small microorganisms in oligotrophic habitats. The surface area can be increased with appendages. Here, we describe chains of interconnecting vesicles protruding from cells of strain Hel3_A1_48, affiliating with Formosa spp. within the Flavo...

journal_title：Applied and environmental microbiology

authors： Fischer T,Schorb M,Reintjes G,Kolovou A,Santarella-Mellwig R,Markert S,Rhiel E,Littmann S,Becher D,Schweder T,Harder J

更新日期：2019-09-17 00:00:00

abstract：:DNA primers corresponding to conserved motifs in bacterial repetitive (REP, ERIC, and BOX) elements and PCR were used to show that REP-, ERIC-, and BOX-like DNA sequences are widely distributed in phytopathogenic Xanthomonas and Pseudomonas strains. REP-, ERIC, and BOX-PCR (collectively known as rep-PCR) were used to ...

journal_title：Applied and environmental microbiology

authors： Louws FJ,Fulbright DW,Stephens CT,de Bruijn FJ

更新日期：1994-07-01 00:00:00

abstract：:Sixty-three strains of lactic streptococci isolated from commercial lactic streptococcal starter cultures were examined for lysogeny by treatment with ultraviolet light or mitomycin C. After treatment with the inducing agent, all strains, whether or not they lysed, were examined for evidence of phage release by electr...

journal_title：Applied and environmental microbiology

authors： Huggins AR,Sandine WE

更新日期：1977-01-01 00:00:00

abstract：:The prevalence of Escherichia coli O157 associated with feedlot cattle in Saskatchewan was determined in a 10-month longitudinal study (3 feedlots) and a point prevalence study (20 feedlots). The prevalence of E. coli O157 at the three different sites in the horizontal study varied from 2.5 to 45%. The point prevalenc...

journal_title：Applied and environmental microbiology

authors： Vidovic S,Korber DR

更新日期：2006-06-01 00:00:00

abstract：:Oxolinic acid (OA), a quinolone, inhibits the activity of DNA gyrase composed of GyrA and GyrB and shows antibacterial activity against Burkholderia glumae. Since B. glumae causes bacterial seedling rot and grain rot of rice, both of which are devastating diseases, the emergence of OA-resistant bacteria has important ...

journal_title：Applied and environmental microbiology

authors： Maeda Y,Kiba A,Ohnishi K,Hikichi Y

更新日期：2004-09-01 00:00:00

abstract：:When mixed ruminal bacteria were inoculated into semicontinuous cultures (25% transfer every other day) containing lactate, dulcitol, pectin, or xylose and Trypticase (1 g/liter) as the sole nitrogen source, the specific activity of ammonia production increased. The greatest enrichment was observed with lactate and xy...

journal_title：Applied and environmental microbiology

authors： Russell JB,Strobel HJ,Chen GJ

更新日期：1988-04-01 00:00:00

abstract：:The purpose of this investigation was to examine the capacity of the biphenyl catabolic enzymes of Comamonas testosteroni B-356 to metabolize dihydroxybiphenyls symmetrically substituted on both rings. Data show that 3,3'-dihydroxybiphenyl is by far the preferred substrate for strain B-356. However, the dihydrodiol me...

journal_title：Applied and environmental microbiology

authors： Sondossi M,Barriault D,Sylvestre M

更新日期：2004-01-01 00:00:00

abstract：:Vibrio parahaemolyticus is indigenous to coastal environments and a frequent cause of seafood-borne gastroenteritis in the United States, primarily due to raw-oyster consumption. Previous seasonal-cycle studies of V. parahaemolyticus have identified water temperature as the strongest environmental predictor. Salinity ...

journal_title：Applied and environmental microbiology

authors： Zimmerman AM,DePaola A,Bowers JC,Krantz JA,Nordstrom JL,Johnson CN,Grimes DJ

更新日期：2007-12-01 00:00:00

abstract：:Ergosterol, a fungus-specific sterol enriched in cell plasma membranes, is an effective antifungal drug target. However, current knowledge of the ergosterol biosynthesis process in the saprophytic human fungal pathogen Aspergillus fumigatus remains limited. In this study, we found that two endoplasmic reticulum-locali...

journal_title：Applied and environmental microbiology

authors： Long N,Xu X,Zeng Q,Sang H,Lu L

更新日期：2017-02-01 00:00:00

abstract：:This study investigated the efficiency of methane and ammonium for stimulating trichloroethylene (TCE) biodegradation in groundwater microcosms (flasks and batch exchange columns) at a psychrophilic temperature (12 degrees C) typical of shallow aquifers in the northern United States or a mesophilic temperature (24 deg...

journal_title：Applied and environmental microbiology

authors： Moran BN,Hickey WJ

更新日期：1997-10-01 00:00:00

abstract：:Auxotrophic microorganisms are often used for genetic engineering, because their biosynthetic deficiency can be complemented by the transforming DNA and allows selection for transformants that have become prototrophic. However, when complementation is obtained by ectopic expression this may lead to unpredictable side ...

journal_title：Applied and environmental microbiology

authors： Nielsen ML,de Jongh WA,Meijer SL,Nielsen J,Mortensen UH

更新日期：2007-11-01 00:00:00

abstract：:The active population of low-affinity methanotrophs in a peat soil microcosm was characterized by stable-isotope probing. "Heavy" (13)C-labeled DNA, produced after microbial growth on (13)CH(4), was separated from naturally abundant (12)C-DNA by cesium chloride density gradient centrifugation and used as a template fo...

journal_title：Applied and environmental microbiology

authors： Morris SA,Radajewski S,Willison TW,Murrell JC

更新日期：2002-03-01 00:00:00

abstract：:Studies of the interaction of Legionella pneumophila with free-living amoebae showed that Naegleria lovaniensis and Acanthamoeba royreba could use L. pneumophila as a sole food source. However, growth of the amoebae on nonnutrient agar plates seeded with L. pneumophila was slower than growth on nonnutrient agar plates...

journal_title：Applied and environmental microbiology

authors： Tyndall RL,Domingue EL

更新日期：1982-10-01 00:00:00

abstract：:Data from analyses of continuous culture fermentation of insoluble cellulose by Ruminococcus albus 7 were used to derive constants for the rate of cellulose hydrolysis and fermentation, growth yield, and maintenance. Cellulose concentration was 1% in the nutrient reservoir, and hydraulic retention times of 0.5, 1.0, 1...

journal_title：Applied and environmental microbiology

authors： Pavlostathis SG,Miller TL,Wolin MJ

更新日期：1988-11-01 00:00:00

abstract：:The human gastrointestinal (GI) tract provides home to a complex microbial community, collectively termed microbiota. Although major efforts have been made to describe the diversity and stability of the microbiota, functional studies have been largely restricted to intestinal isolates and include few community studies...

journal_title：Applied and environmental microbiology

authors： Booijink CC,Boekhorst J,Zoetendal EG,Smidt H,Kleerebezem M,de Vos WM

更新日期：2010-08-01 00:00:00

abstract：:Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcrip...

journal_title：Applied and environmental microbiology

authors： Skraber S,Langlet J,Kremer JR,Mossong J,De Landtsheer S,Even J,Muller CP,Hoffmann L,Cauchie HM

更新日期：2011-08-01 00:00:00

abstract：:A multilocus enzyme electrophoresis analysis of 96 strains of the salmonellae distinguished 80 electrophoretic types (ETs) and placed them in eight groups, seven of which correspond precisely to the seven taxonomic groups (I, II, IIIa, IIIb, IV, V, and VI) previously defined on the basis of biotype and genomic DNA hyb...

journal_title：Applied and environmental microbiology

authors： Boyd EF,Wang FS,Whittam TS,Selander RK

更新日期：1996-03-01 00:00:00

abstract：:The genus Exiguobacterium can adapt readily to, and survive in, diverse environments. Our study demonstrated that Exiguobacterium sp. strain S3-2, isolated from marine sediment, is resistant to five antibiotics. The plasmid pMC1 in this strain carries seven putative resistance genes. We functionally characterized thes...

journal_title：Applied and environmental microbiology

authors： Yang J,Wang C,Wu J,Liu L,Zhang G,Feng J

更新日期：2014-02-01 00:00:00

abstract：:The gene encoding serine alkaline protease (SapSh) of the psychrotrophic bacterium Shewanella strain Ac10 was cloned in Escherichia coli. The amino acid sequence deduced from the 2,442-bp nucleotide sequence revealed that the protein was 814 amino acids long and had an estimated molecular weight of 85,113. SapSh exhib...

journal_title：Applied and environmental microbiology

authors： Kulakova L,Galkin A,Kurihara T,Yoshimura T,Esaki N

更新日期：1999-02-01 00:00:00

abstract：:We analyzed the communities of soil basidiomycetes in agroecosystems that differ in tillage history at the Kellogg Biological Station Long-Term Ecological Research site near Battle Creek, Michigan. The approach combined soil DNA extraction through a bead-beating method modified to increase recovery of fungal DNA, PCR ...

journal_title：Applied and environmental microbiology

authors： Lynch MD,Thorn RG

更新日期：2006-11-01 00:00:00

abstract：:16S rRNA gene-targeted group-specific primers were designed and validated for specific detection and quantification of the Clostridium leptum subgroup and the Atopobium cluster. To monitor the predominant bacteria in human feces by real-time PCR, we used these specific primers together with four sets of group-specific...

journal_title：Applied and environmental microbiology

authors： Matsuki T,Watanabe K,Fujimoto J,Takada T,Tanaka R

更新日期：2004-12-01 00:00:00

abstract：:Pierce's disease is a devastating lethal disease of Vitus vinifera grapevines caused by the bacterium Xylella fastidiosa. There is no cure for Pierce's disease, and control is achieved predominantly by suppressing transmission of the glassy-winged sharpshooter insect vector. We present a simple robust approach for the...

journal_title：Applied and environmental microbiology

authors： Azizi A,Arora A,Markiv A,Lampe DJ,Miller TA,Kang AS

更新日期：2012-04-01 00:00:00

abstract：:A new bacterial pathogen of chinook salmon (oncorhynchus tshawytscha) was isolated from fish in Oregon's Rogue River. The bacteria are biochemically and serologically related to strains of Edwardsiella tarda. Initially isolated from chinook salmon, the bacteria were also pathogenic for steelhead and rainbow trout (Sal...

journal_title：Applied and environmental microbiology

authors： Amandi A,Hiu SF,Rohovec JS,Fryer JL

更新日期：1982-06-01 00:00:00

abstract：:A soil-inhabiting Fusarium proliferatum strain was capable of transforming or degrading nonlabeled and (sup14)C-labeled industrial, natural, and synthetic lignin. The mineralization rate per day (expressed as the percentage of added radioactivity recovered as to (sup14)CO(inf2)) was maximal during primary metabolism. ...

journal_title：Applied and environmental microbiology

authors： Regalado V,Rodriguez A,Perestelo F,Carnicero A,De La Fuente G,Falcon MA

更新日期：1997-09-01 00:00:00

abstract：:Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not ...

journal_title：Applied and environmental microbiology

authors： Camper AK,McFeters GA

更新日期：1979-03-01 00:00:00

abstract：:The objective of this study was to explore the long-term effects of different organic and inorganic fertilizers on activity and composition of the denitrifying and total bacterial communities in arable soil. Soil from the following six treatments was analyzed in an experimental field site established in 1956: cattle m...

journal_title：Applied and environmental microbiology

authors： Enwall K,Philippot L,Hallin S

更新日期：2005-12-01 00:00:00

abstract：:Reductive and oxidative pathways of the sulfur cycle were studied in a marine sediment by parallel radiotracer experiments with SO(4), H(2)S, and S(2)O(3) injected into undisturbed sediment cores. The distributions of viable populations of sulfate- and thiosulfate-reducing bacteria and of thiosulfate-disproportionatin...

journal_title：Applied and environmental microbiology

authors： Jørgensen BB,Bak F

更新日期：1991-03-01 00:00:00

abstract：:Virulence gene profiles of atypical enteropathogenic Escherichia coli (aEPEC) and Shiga toxin-producing E. coli (STEC) from cattle, sheep, and humans were examined to determine the relationship between pathotypes. Shared virulence factors (intimin, EHEC hemolysin, serine protease, and a type II secretion system) were ...

journal_title：Applied and environmental microbiology

authors： Cookson AL,Cao M,Bennett J,Nicol C,Thomson-Carter F,Attwood GT

更新日期：2010-06-01 00:00:00

abstract：:By using cryo-scanning electron microscopy and quantification with lectin-conjugated probes, we have detected the production of exopolysaccharides (EPS) in Bifidobacterium animalis subsp. lactis in the presence of bile. In addition, the expression of gtf01207, which codifies a putative priming glycosyltransferase invo...

journal_title：Applied and environmental microbiology

authors： Ruas-Madiedo P,Gueimonde M,Arigoni F,de los Reyes-Gavilán CG,Margolles A

更新日期：2009-02-01 00:00:00