Cocultivation of Legionella pneumophila and free-living amoebae.

Abstract:

:Studies of the interaction of Legionella pneumophila with free-living amoebae showed that Naegleria lovaniensis and Acanthamoeba royreba could use L. pneumophila as a sole food source. However, growth of the amoebae on nonnutrient agar plates seeded with L. pneumophila was slower than growth on nonnutrient agar plates seeded with Escherichia coli. On inoculation of L. pneumophila into axenic cultures of N. lovaniensis and A. royreba, 99.9% of the L. pneumophila was destroyed within 24 h. After several weeks, however, some amoeba cultures became chronically infected and supported the growth of L. pneumophila. Amoebae exposed to L. pneumophila and containing adhered L. pneumophila, L. pneumophila antigens, or both, showed no increased pathogenic potential on intranasal inoculation of weanling mice. Similarly, L. pneumophila propagated in chronically infected amoeba cultures showed no increase in virulence on intraperitoneal inoculation of guinea pigs relative to L. pneumophila grown in yeast extract broth.

journal_name

Appl Environ Microbiol

authors

Tyndall RL,Domingue EL

doi

10.1128/AEM.44.4.954-959.1982

subject

Has Abstract

pub_date

1982-10-01 00:00:00

pages

954-9

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

44

pub_type

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