Abstract:
:A four-step flavanone biosynthetic pathway was constructed and introduced into Saccharomyces cerevisiae. The recombinant yeast strain was fed with phenylpropanoid acids and produced the flavanones naringenin and pinocembrin 62 and 22 times more efficiently compared to previously reported recombinant prokaryotic strains. Microbial biosynthesis of the flavanone eriodictyol was also achieved.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Yan Y,Kohli A,Koffas MAdoi
10.1128/AEM.71.9.5610-5613.2005keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
5610-3issue
9eissn
0099-2240issn
1098-5336pii
71/9/5610journal_volume
71pub_type
杂志文章abstract::Thirty-one urease-positive thermophilic Campylobacter (UPTC) isolates, including three reference strains (NCTC12892, NCTC12895 and NCTC12896), and three Campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (MLEE). We...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.6.3308-3310.2003
更新日期:2003-06-01 00:00:00
abstract::In our previous study, Bacillus subtilis strain BSK3S, containing a polymyxin biosynthetic gene cluster from Paenibacillus polymyxa, could produce polymyxin only in the presence of exogenously added L-2,4-diaminobutyric acid (Dab). The dependence of polymyxin production on exogenous Dab was removed by introducing an e...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.07912-11
更新日期:2012-06-01 00:00:00
abstract::Based on results from eight experiments, no overall difference was found between roll tube and three- and five-tube most-probable-number (MPN) methods for estimating total numbers of ruminal bacteria. However, standard errors for the replicate means within an experiment were higher with the MPN procedures. Visual grow...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.11.2789-2792.1989
更新日期:1989-11-01 00:00:00
abstract::The presence and activities of urease genes were investigated in 49 clinical, food, and environmental Bacillus cereus isolates. Ten strains were shown to have urease genes, with eight of these strains showing growth on urea as the sole nitrogen source. Two of the urease-positive strains, including the sequenced strain...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02737-07
更新日期:2008-04-01 00:00:00
abstract:UNLABELLED:Mycoplasmas are minimal, wall-less bacteria but have retained the ability to secrete complex carbohydrate polymers that constitute a glycocalyx. In members of the Mycoplasma mycoides cluster, which are important ruminant pathogens, the glycocalyx includes both cell-attached and cell-free polysaccharides. Thi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00274-16
更新日期:2016-05-16 00:00:00
abstract::The pressure resistances of the spores of six Bacillus strains were examined at 5 to 10(deg)C and were compared with their heat resistances. The pressure treatments (at 981 MPa for 40 min and at 588 MPa for 120 min) did not inactivate the spores of B. stearothermophilus IAM12043, B. subtilis IAM12118, and B. lichenifo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.10.3897-3900.1996
更新日期:1996-10-01 00:00:00
abstract::Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.2.560-562.1990
更新日期:1990-02-01 00:00:00
abstract::A dot blot hybridization procedure with 32P-labeled whole chromosomal DNA of the type strains as probes was developed as a rapid and simple method for identification of intestinal Bacteroides species. Bacterial cells were fixed onto membrane filters by slight suction, treated with 0.5 N NaOH, and hybridized with these...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.5.1158-1162.1988
更新日期:1988-05-01 00:00:00
abstract::Magnetotactic bacteria comprise a phylogenetically diverse group that is capable of synthesizing intracellular magnetic particles. Although various morphotypes of magnetotactic bacteria have been observed in the environment, bacterial strains available in pure culture are currently limited to a few genera due to diffi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02124-09
更新日期:2010-03-01 00:00:00
abstract::Bacterial signaling is an important part of community life, but little is known about the signal transduction pathways of the as-yet-uncultured members of microbial communities. To address this gap, we aimed to identify genes directing the synthesis of signals in uncultured bacteria associated with the midguts of gyps...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02617-06
更新日期:2007-06-01 00:00:00
abstract::Heterotrophic bacteria in the SAR11 and Roseobacter lineages shape the marine carbon, nitrogen, phosphorous, and sulfur cycles, yet they do so having adopted divergent ecological strategies. Currently, it is unknown whether these globally significant groups partition into specific niches with respect to micronutrients...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03128-15
更新日期:2016-01-04 00:00:00
abstract::The Chloroflexi CL500-11 clade contributes a large proportion of the bacterial biomass in the oxygenated hypolimnia of deep lakes worldwide, including the world's largest freshwater system, the Laurentian Great Lakes. Traits that allow CL500-11 to thrive and its biogeochemical role in these environments are currently ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03014-15
更新日期:2015-12-18 00:00:00
abstract::The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop egg...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.7.4052-4056.2005
更新日期:2005-07-01 00:00:00
abstract::Oil field bacteria were characterized by cloning and sequencing of PCR-amplified 16S rRNA genes. A variety of gram-negative, sulfate-reducing bacteria was detected (16 members of the family Desulfovibrionaceae and 8 members of the family Desulfobacteriaceae). In contrast, a much more limited number of anaerobic, ferme...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.5.1623-1629.1996
更新日期:1996-05-01 00:00:00
abstract::Bacterial utilization of crude oil components, such as the n-alkanes, requires complex cell surface adaptation to allow adherence to oil. To better understand microbial cell surface adaptation to growth on crude oil, the cell surface characteristics of two Pseudomonas aeruginosa strains, U1 and U3, both isolated from ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.10.5096-5103.2002
更新日期:2002-10-01 00:00:00
abstract::Eubacterium lentum is a gram-positive, nonsporeforming, nonmotile, asaccharolytic anaerobe. In the present investigations, 3 E. lentum strains (group E) isolated from rat feces were compared with 30 E. lentum strains (groups A, B, C, and D) previously studied by Macdonald et al. (I. A. Macdonald, J. F. Jellet, D. E. M...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.1.39-43.1984
更新日期:1984-01-01 00:00:00
abstract::The effects of various nitrogen sources and of the cultivation time on the biosynthesis of N-labelled azoverdin were studied in batch cultures of Azomonas macrocytogenes ATCC 12334. Growth for 48 h in the presence of ammonium sulfate (0.2 g/liter) resulted in 95% labelling of azoverdin with N. A growth-related synthes...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.12.4605-4607.1994
更新日期:1994-12-01 00:00:00
abstract::In two intertidal marshes, the vertical distribution in the sediment and inhibition by ammonium of methane oxidation were investigated by slurry incubation experiments. The two sites differ in their dominant vegetation type, i.e., reed and bulrush, and in their heights above sea level. The reed site was elevated with ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.12.4734-4740.1997
更新日期:1997-12-01 00:00:00
abstract::Vibrio parahaemolyticus and Vibrio vulnificus, which are native to estuaries globally, are agents of seafood-borne or wound infections, both potentially fatal. Like all vibrios autochthonous to coastal regions, their abundance varies with changes in environmental parameters. Sea surface temperature (SST), sea surface ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01296-12
更新日期:2012-10-01 00:00:00
abstract::We examined the influence of elevated CO2 concentration on denitrifier enzyme activity in wheat rhizoplanes by using controlled environments and solution culture techniques. Potential denitrification activity was from 3 to 24 times higher on roots that were grown under an elevated CO2 concentration of 1,000 micromoles...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.11.4621-4624.1997
更新日期:1997-11-01 00:00:00
abstract::Ophthalmic acid (OA; l-γ-glutamyl-l-2-aminobutyryl-glycine) is an analog of glutathione (GSH; l-γ-glutamyl-l-cysteinyl-glycine) in which the cysteine moiety is replaced by l-2-aminobutyrate. OA is a useful peptide for the pharmaceutical and/or food industries. Herein, we report a method for the production of OA using ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02806-17
更新日期:2018-03-19 00:00:00
abstract::The aim of this work was to investigate the medium requirements for growth and production of exopolysaccharides by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772. The strain was grown in batch cultures on a chemically defined medium, and the technique of single omission of medium components was applied to deter...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.4.1333-1337.1998
更新日期:1998-04-01 00:00:00
abstract::A restriction fragment length polymorphism analysis of nuclear ribosomal RNA genes (rDNA) was used to measure the amount and distribution of genetic variability in populations of the Japanese pear pathotype of Alternaria alternata on both micro- and macrogeographical scales. A total of 322 isolates were obtained from ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.10.3197-3205.1993
更新日期:1993-10-01 00:00:00
abstract::We examined nitrate-dependent Fe(2+) oxidation mediated by anaerobic ammonium oxidation (anammox) bacteria. Enrichment cultures of "Candidatus Brocadia sinica" anaerobically oxidized Fe(2+) and reduced NO3(-) to nitrogen gas at rates of 3.7 ± 0.2 and 1.3 ± 0.1 (mean ± standard deviation [SD]) nmol mg protein(-1) min(-...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00743-13
更新日期:2013-07-01 00:00:00
abstract::Graded doses of pure ochratoxin A (0, 0.5, 1.0, 2.0, 4.0, and 8.0 microgram of toxin per g of feed) were incorporated into a commercial diet which was fed to chickens from hatching to 3 weeks of age, at which time the experiments were terminated. Liver glycogen levels were elevated significantly (P less than 0.05) by ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.37.1.122-126.1979
更新日期:1979-01-01 00:00:00
abstract::A technique to determine which nutrients limit bacterial growth in soil was developed. The method was based on measuring the thymidine incorporation rate of bacteria after the addition of C, N, and P in different combinations to soil samples. First, the thymidine incorporation method was tested in two different soils:...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.4.1830-1838.2001
更新日期:2001-04-01 00:00:00
abstract::Production of propionicin PLG-1 by Propionibacterium thoenii P127 was pH dependent, with maximal activity detected in supernatants of cultures grown at pH 7.0 Propionicin PLG-1 was purified by ion-exchange chromatography and isoelectric focusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of propionicin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.1.83-88.1993
更新日期:1993-01-01 00:00:00
abstract::Soils contain a tangle of minerals, water, nutrients, gases, plant roots, decaying organic matter, and microorganisms which work together to cycle nutrients and support terrestrial plant growth. Most soil microorganisms live in periodically interconnected communities closely associated with soil aggregates, i.e., smal...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章,评审
doi:10.1128/AEM.00324-19
更新日期:2019-07-01 00:00:00
abstract::Pierce's disease is a devastating lethal disease of Vitus vinifera grapevines caused by the bacterium Xylella fastidiosa. There is no cure for Pierce's disease, and control is achieved predominantly by suppressing transmission of the glassy-winged sharpshooter insect vector. We present a simple robust approach for the...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.07807-11
更新日期:2012-04-01 00:00:00
abstract:UNLABELLED:The l-isoaspartyl protein carboxyl methyltransferase (PCM) repairs protein damage resulting from spontaneous conversion of aspartyl or asparaginyl residues to isoaspartate and increases long-term stationary-phase survival of Escherichia coli under stress. In the course of studies intended to examine PCM func...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00623-16
更新日期:2016-08-15 00:00:00