Most-probable-number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium.

abstract：:A large, obligately anaerobic spirochete (strain PB) was isolated from bovine rumen fluid by a procedure involving rifampin as a selective agent. The helical cells measured 0.6 to 0.7 micron by 12 to 20 micron and possessed approximately 16 periplasmic flagella inserted near each end of the protoplasmic cylinder. The ...

journal_title：Applied and environmental microbiology

authors： Paster BJ,Canale-Parola E

更新日期：1985-08-01 00:00:00

abstract：:A novel sulfate-reducing bacterium isolated from fuel-contaminated subsurface soil, strain PRTOL1, mineralizes toluene as the sole electron donor and carbon source under strictly anaerobic conditions. The mineralization of 80% of toluene carbon to CO2 was demonstrated in experiments with [ring-U-14C]toluene; 15% of to...

journal_title：Applied and environmental microbiology

authors： Beller HR,Spormann AM,Sharma PK,Cole JR,Reinhard M

更新日期：1996-04-01 00:00:00

abstract：:Acetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions, Clostridium acetobutylicum ATCC 824 (and strains derived from it) generates both d- and l-stereoisomers of acetoin, but because of the absence of an ACR enzyme, it does not produce 2,3-butanediol. A gene encoding A...

journal_title：Applied and environmental microbiology

authors： Siemerink MA,Kuit W,López Contreras AM,Eggink G,van der Oost J,Kengen SW

更新日期：2011-04-01 00:00:00

abstract：:Minute amounts of oxygen were supplied to a continuous cultivation of Lactococcus lactis subsp. cremoris MG1363 grown on a defined glucose-limited medium at a dilution rate of 0.1 h(-1). More than 80% of the carbon supplied with glucose ended up in fermentation products other than lactate. Addition of even minute amou...

journal_title：Applied and environmental microbiology

authors： Jensen NB,Melchiorsen CR,Jokumsen KV,Villadsen J

更新日期：2001-06-01 00:00:00

abstract：:Kinetic parameters and the role of cytochrome c(3) in sulfate, Fe(III), and U(VI) reduction were investigated in Desulfovibrio vulgaris Hildenborough. While sulfate reduction followed Michaelis-Menten kinetics (K(m) = 220 micro M), loss of Fe(III) and U(VI) was first-order at all concentrations tested. Initial reducti...

journal_title：Applied and environmental microbiology

authors： Elias DA,Suflita JM,McInerney MJ,Krumholz LR

更新日期：2004-01-01 00:00:00

abstract：:Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

journal_title：Applied and environmental microbiology

authors： Sjogren RE,Gibson MJ

更新日期：1981-06-01 00:00:00

abstract：:The evolution of the microbial spoilage population for air- and vacuum-packaged meat (beef and pork) stored at 4 degrees C was investigated over 11 days. We monitored the viable counts (mesophilic total aerobic bacteria, Pseudomonas spp., Enterobacteriaceae, lactic acid bacteria, and Enterococcus spp.) by the microbio...

journal_title：Applied and environmental microbiology

authors： Mayr D,Margesin R,Klingsbichel E,Hartungen E,Jenewein D,Schinner F,Märk TD

更新日期：2003-08-01 00:00:00

abstract：:Campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. This procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of C. jejuni in milk from bulk tanks. ...

journal_title：Applied and environmental microbiology

authors： Lovett J,Francis DW,Hunt JM

更新日期：1983-08-01 00:00:00

abstract：:A monoclonal antibody designated 302 beta that is specific for the beta subunit of the oxygenase component (ISPTOL) of toluene dioxygenase from Pseudomonas putida F1 was used to prepare an immunoaffinity column. ISPTOL in cell extracts of Escherichia coli JM109(pDTG611) bound to the column, and an enzyme-linked immuno...

journal_title：Applied and environmental microbiology

authors： Lynch NA,Jiang H,Gibson DT

更新日期：1996-06-01 00:00:00

abstract：:Genome-wide analysis has revealed abundant FabG (beta-ketoacyl-ACP reductase) paralogs, with uncharacterized biological functions, in several halophilic archaea. In this study, we identified for the first time that the fabG1 gene, but not the other five fabG paralogs, encodes the polyhydroxyalkanoate (PHA)-specific ac...

journal_title：Applied and environmental microbiology

authors： Han J,Lu Q,Zhou L,Liu H,Xiang H

更新日期：2009-10-01 00:00:00

abstract：:Lipophilic compounds of the culture suspension containing Rhodococcus erythropolis DSM43215 had surfactant properties when the bacteria were cultivated with n-alkanes as the sole carbon source. Thirteen main components from a dichloromethane-methanol extract of the R. erythropolis cultures were isolated and characteri...

journal_title：Applied and environmental microbiology

authors： Kretschmer A,Bock H,Wagner F

更新日期：1982-10-01 00:00:00

abstract：:NAD+-dependent formate dehydrogenase (FDH; EC 1.2.1.2) is an industrial enzyme widely used for NADH regeneration. However, enzyme inactivation caused by the oxidation of cysteine residues is a flaw of native FDH. In this study, we relieved the oxidation of the free cysteine of FDH from Candida boidinii (CboFDH) throug...

journal_title：Applied and environmental microbiology

authors： Zheng J,Yang T,Zhou J,Xu M,Zhang X,Rao Z

更新日期：2016-12-30 00:00:00

abstract：:Investigations concerning the role of distribution system biofilms on water quality were conducted at a drinking water utility in New Jersey. The utility experienced long-term bacteriological problems in the distribution system, while treatment plant effluents were uniformly negative for coliform bacteria. Results of ...

journal_title：Applied and environmental microbiology

authors： LeChevallier MW,Babcock TM,Lee RG

更新日期：1987-12-01 00:00:00

abstract：:Biotransformation plays an increasingly important role in the industrial production of fine chemicals due to its high product specificity and low energy requirement. One challenge in biotransformation is the toxicity of substrates and/or products to biocatalytic microorganisms and enzymes. Biofilms are known for their...

journal_title：Applied and environmental microbiology

authors： Li XZ,Webb JS,Kjelleberg S,Rosche B

更新日期：2006-02-01 00:00:00

abstract：:Vegetative cells of Bacillus cereus were subjected to low doses of nisin (0.06 microg/ml) and mild pulsed-electric field treatment (16.7 kV/cm, 50 pulses each of 2-micros duration). Combining both treatments resulted in a reduction of 1.8 log units more than the sum of the reductions obtained with the single treatment...

journal_title：Applied and environmental microbiology

authors： Pol IE,Mastwijk HC,Bartels PV,Smid EJ

更新日期：2000-01-01 00:00:00

abstract：:While some species in the Bacillus cereus group are well-characterized human pathogens (e.g., B. anthracis and B. cereus sensu stricto), the pathogenicity of other species (e.g., B. pseudomycoides) either has not been characterized or is presently not well understood. To provide an updated characterization of the path...

journal_title：Applied and environmental microbiology

authors： Miller RA,Jian J,Beno SM,Wiedmann M,Kovac J

更新日期：2018-03-01 00:00:00

abstract：:Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps ...

journal_title：Applied and environmental microbiology

authors： Tyndall RL,Hand RE Jr,Mann RC,Evans C,Jernigan R

更新日期：1985-04-01 00:00:00

abstract：:Mannitol, a six-carbon sugar alcohol, is the main storage carbon in the button mushroom, Agaricus bisporus. Given the physiological importance of mannitol metabolism in growth, fruit body development, and salt tolerance of A. bisporus, the enzyme responsible for mannitol biosynthesis, NADP-dependent mannitol dehydroge...

journal_title：Applied and environmental microbiology

authors： Stoop JM,Mooibroek H

更新日期：1998-12-01 00:00:00

abstract：:The biological succession that occurs during the first year of life in the gut of infants in Western countries is broadly predictable in terms of the increasing complexity of the composition of microbiotas. Less information is available about microbiotas in Asian countries, where environmental, nutritional, and cultur...

journal_title：Applied and environmental microbiology

authors： Lawley B,Otal A,Moloney-Geany K,Diana A,Houghton L,Heath AM,Taylor RW,Tannock GW

更新日期：2019-09-17 00:00:00

abstract：:Resting cells of Micrococcus luteus have been shown to remove strontium (Sr) from dilute aqueous solutions of SrCl(2) at pH 7. Loadings of 25 mg of Sr per g of cell dry weight were achieved by cells exposed to a solution containing 50 ppm (mg/liter) of Sr. Sr binding occurred in the absence of nutrients and did not re...

journal_title：Applied and environmental microbiology

authors： Faison BD,Cancel CA,Lewis SN,Adler HI

更新日期：1990-12-01 00:00:00

abstract：:The growth characteristics and acetate production of several Escherichia coli strains were compared by using shake flasks, batch fermentations, and glucose-feedback-controlled fed-batch fermentations to assess the potential of each strain to grow at high cell densities. Of the E. coli strains tested, including JM105, ...

journal_title：Applied and environmental microbiology

authors： Luli GW,Strohl WR

更新日期：1990-04-01 00:00:00

abstract：:Biodesulfurization was monitored in a recombinant Pseudomonas putida CECT5279 strain. DszB desulfinase activity reached a sharp maximum at the early exponential phase, but it rapidly decreased at later growth phases. A model two-step resting-cell process combining sequentially P. putida cells from the late and early e...

journal_title：Applied and environmental microbiology

authors： Calzada J,Zamarro MT,Alcón A,Santos VE,Díaz E,García JL,Garcia-Ochoa F

更新日期：2009-02-01 00:00:00

abstract：:We tested the sensitivity of coastal picophytoplankton exposed to natural sunlight in short-term experiments. Cell abundance and cell-specific chlorophyll fluorescence were significantly reduced in Prochlorococcus spp. but not in Synechococcus, whereas picoeukaryotes had an intermediate response. These results are the...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Hofer JS,Alonso-Sáez L,Gasol JM

更新日期：2005-04-01 00:00:00

abstract：:Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway of ethane oxidation via ethanol, acetaldehyde, and acetic acid. In vitro studies indicated that ethane-oxidizing activi...

journal_title：Applied and environmental microbiology

authors： Davies JS,Wellman AM,Zajic JE

更新日期：1976-07-01 00:00:00

abstract：:This work presents data on the application of a bacterial luciferase used to monitor gene expression of Streptococcus thermophilus in the digestive tract. The main result is that the bacterium was able to produce an active beta-galactosidase in the digestive tract, although it did not multiply during its transit. This...

journal_title：Applied and environmental microbiology

authors： Drouault S,Anba J,Corthier G

更新日期：2002-02-01 00:00:00

abstract：:The effects of UV-B radiation on the heterotrophic nanoflagellate Bodo saltans (Kinetoplastida) were examined under controlled conditions with artificial UV sources and also under natural solar radiation in an oligotrophic lake. In both types of experiments, the characteristic elongated cell morphology of this flagell...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Oberleiter A,Psenner R

更新日期：1996-12-01 00:00:00

abstract：:The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film electrochemical flow cell. The influence of the electrochemical potential was evaluated at two different ionic strengths (0.01 and 0.1 M NaCl; pH...

journal_title：Applied and environmental microbiology

authors： Busalmen JP,de Sánchez SR

更新日期：2001-07-01 00:00:00

abstract：:A granulovirus (GV) isolated from Epinotia aporema (Lepidoptera: Tortricidae)-a major soybean pest-was studied in terms of its main morphological, biochemical, and biological properties. The ovoidal occlusion bodies were 466 by 296 nm in size, and their most prominent protein had an apparent molecular mass of 29 kDa. ...

journal_title：Applied and environmental microbiology

authors： Sciocco-Cap A,Parola AD,Goldberg AV,Ghiringhelli PD,Romanowski V

更新日期：2001-08-01 00:00:00

abstract：:Information on the interaction between mixed populations in the rumen and plant phenolics is required to fully elucidate the limitations of phenolic compounds on forage digestibility. The objective of this study was to examine the degradation of Italian ryegrass (Lolium multiflorum L.) hay incubated with mixed ruminal...

journal_title：Applied and environmental microbiology

authors： Theodorou MK,Gascoyne DJ,Akin DE,Hartley RD

更新日期：1987-05-01 00:00:00

abstract：:DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

journal_title：Applied and environmental microbiology

authors： Coleman SS,Melanson DM,Biosca EG,Oliver JD

更新日期：1996-04-01 00:00:00