Treponema saccharophilum sp. nov., a large pectinolytic spirochete from the bovine rumen.

abstract：:An amylosucrase gene was subjected to high-rate segmental random mutagenesis, which was directed toward a segment encoding amino acids that influence the interaction with substrate molecules in subsites -1 to +3. A screen was used to identify enzyme variants with compromised glucan chain elongation. With an average mu...

journal_title：Applied and environmental microbiology

authors： Schneider J,Fricke C,Overwin H,Hofmann B,Hofer B

更新日期：2009-12-01 00:00:00

abstract：:In earlier studies, the pathway of toluene and m- and p-xylene degradation (TOL pathway) in Pseudomonas putida (pWW0) was found to be subject to catabolite repression when the strain was grown at the maximal rate on glucose or succinate in the presence of an inducer. This report describes catabolite repression of the ...

journal_title：Applied and environmental microbiology

authors： Duetz WA,Marqués S,Wind B,Ramos JL,van Andel JG

更新日期：1996-02-01 00:00:00

abstract：:This study was performed to determine the effects of feeding a fiber-rich fraction of Brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (SCFA) production in normal mice. The boiled-water-insoluble fraction of Brassica rapa L. (nozawana), which consists mainly of ...

journal_title：Applied and environmental microbiology

authors： Tanaka S,Yamamoto K,Yamada K,Furuya K,Uyeno Y

更新日期：2016-04-18 00:00:00

abstract：:Several transposable elements were isolated from the genome of Xanthomonas oryzae pv. oryzae. These elements and an avirulence gene isolated from X. oryzae pv. oryzae were used as hybridization probes for a collection of X. oryzae pv. oryzae strains from the Philippines. Each of the sequences was present in multiple c...

journal_title：Applied and environmental microbiology

authors： Nelson RJ,Baraoidan MR,Cruz CM,Yap IV,Leach JE,Mew TW,Leung H

更新日期：1994-09-01 00:00:00

abstract：:Salmonella enterica serovar Typhimurium was grown at salt concentrations ranging from 0.5 to 7.5% in minimal medium with and without added osmoprotectant and in a rich medium. In minimal medium, the cells showed an initial decline period, and consequently the definition of the lag time of the resultant log count curve...

journal_title：Applied and environmental microbiology

authors： Zhou K,George SM,Métris A,Li PL,Baranyi J

更新日期：2011-03-01 00:00:00

abstract：:Total DNA from 21 collection or industrial Bifidobacterium strains was cleaved with various restriction endonucleases. Following electrophoresis, the fragments were subjected to Southern blot hybridization with a heterologous [alpha-32P]dCTP-labeled rDNA (genes coding for rRNA) 23S gene probe. The ribosomal patterns a...

journal_title：Applied and environmental microbiology

authors： Mangin I,Bourget N,Bouhnik Y,Bisetti N,Simonet JM,Decaris B

更新日期：1994-05-01 00:00:00

abstract：:The effects of various nitrogen sources and of the cultivation time on the biosynthesis of N-labelled azoverdin were studied in batch cultures of Azomonas macrocytogenes ATCC 12334. Growth for 48 h in the presence of ammonium sulfate (0.2 g/liter) resulted in 95% labelling of azoverdin with N. A growth-related synthes...

journal_title：Applied and environmental microbiology

authors： Dobisová M,Abdallah MA,Kyslík P

更新日期：1994-12-01 00:00:00

abstract：:A high-throughput method which is applicable for rapid screening, identification, and delineation of isolates of Vibrio cholerae, sensitive to genome variation, and capable of providing phylogenetic inferences enhances environmental monitoring of this bacterium. We have developed and optimized a method for genomic fin...

journal_title：Applied and environmental microbiology

authors： Chokesajjawatee N,Zo YG,Colwell RR

更新日期：2008-09-01 00:00:00

abstract：:Yeast extract agar, pork infusion agar, and modifications of these media were used to recover heated Clostridium botulinum spores. The D- and z-values were determined. Two type A strains and one type B strain of C. botulinum were studied. In all cases the D-values were largest when the spores were recovered in yeast e...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Pflug IJ

更新日期：1977-10-01 00:00:00

abstract：:Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We pr...

journal_title：Applied and environmental microbiology

authors： Hashimoto Si,Katsumata R

更新日期：1999-06-01 00:00:00

abstract：:A long-term epidemiological study of Cryptosporidium molnari in aquacultured European sea bass (ESB) and gilthead sea bream (GSB) was performed in different types of facilities on the Atlantic, Cantabric, and Mediterranean coasts. Four types of studies were carried out. In study A, fish raised from juveniles to market...

journal_title：Applied and environmental microbiology

authors： Sitjà-Bobadilla A,Padrós F,Aguilera C,Alvarez-Pellitero P

更新日期：2005-01-01 00:00:00

abstract：:Polyclonal antibodies of predetermined specificity for pediocin PA-1 (pedA1) have been generated by immunization of rabbits with a chemically synthesized C-terminal fragment of this bacteriocin (PH2) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and specificity of the PH2-KLH-gener...

journal_title：Applied and environmental microbiology

authors： Martínez JM,Martínez MI,Suárez AM,Herranz C,Casaus P,Cintas LM,Rodríguez JM,Hernández PE

更新日期：1998-11-01 00:00:00

abstract：:To produce high levels of β-lactams, the filamentous fungus Penicillium rubens (previously named Penicillium chrysogenum) has been subjected to an extensive classical strain improvement (CSI) program during the last few decades. This has led to the accumulation of many mutations that were spread over the genome. Detai...

journal_title：Applied and environmental microbiology

authors： Wu M,Crismaru CG,Salo O,Bovenberg RAL,Driessen AJM

更新日期：2020-01-21 00:00:00

abstract：:Evaluation of the fate and transport of biological warfare (BW) agents in landfills requires the development of specific and sensitive detection assays. The objective of the current study was to develop and validate SYBR green quantitative real-time PCR (Q-PCR) assays for the specific detection and quantification of s...

journal_title：Applied and environmental microbiology

authors： Saikaly PE,Barlaz MA,de Los Reyes FL 3rd

更新日期：2007-10-01 00:00:00

abstract：:Stored sugarbeet roots began fermenting within 24 h after oxygen was depleted at 26 C when the resident bacterial populations increased dramatically. Most of the bacteria present after anaerobic storage for 7 days at 26 C could hydrolyze sucrose in vitro. Although pH and sucrose levels decreased and invert sugar level...

journal_title：Applied and environmental microbiology

authors： Cole DF,Bugbee WM

更新日期：1976-05-01 00:00:00

abstract：:2-Methyl-6-ethylaniline (MEA) is the main microbial degradation intermediate of the chloroacetanilide herbicides acetochlor and metolachlor. Sphingobium sp. strain MEA3-1 can utilize MEA and various alkyl-substituted aniline and phenol compounds as sole carbon and energy sources for growth. We isolated the mutant stra...

journal_title：Applied and environmental microbiology

authors： Dong W,Chen Q,Hou Y,Li S,Zhuang K,Huang F,Zhou J,Li Z,Wang J,Fu L,Zhang Z,Huang Y,Wang F,Cui Z

更新日期：2015-12-01 00:00:00

abstract：:Escherichia coli O157:H7 causes hemorrhagic colitis and life-threatening complications. Because healthy cattle are reservoirs for the bacterium, ruminant infection models have applications in analyzing the relationship between cattle and this human pathogen and in testing interventions to reduce or prevent bovine colo...

journal_title：Applied and environmental microbiology

authors： Sheng H,Davis MA,Knecht HJ,Hovde CJ

更新日期：2004-08-01 00:00:00

abstract：:Noroviruses, which are members of the Caliciviridae family, represent the leading cause of nonbacterial gastroenteritis in developed countries; such norovirus infections result in high economic costs for health protection. Person-to-person contact, contaminated water, and foods, especially raw shellfish, vegetables, a...

journal_title：Applied and environmental microbiology

authors： Buckow R,Isbarn S,Knorr D,Heinz V,Lehmacher A

更新日期：2008-02-01 00:00:00

abstract：:The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Ghazally S,Walter J,Loach D,Brooks H,Cook G,Surette M,Simmers C,Bremer P,Dal Bello F,Hertel C

更新日期：2005-12-01 00:00:00

abstract：:Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distributi...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Heijnen L,van der Kooij D

更新日期：2013-10-01 00:00:00

abstract：:Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by het...

journal_title：Applied and environmental microbiology

authors： van der Kaaij H,Desiere F,Mollet B,Germond JE

更新日期：2004-03-01 00:00:00

abstract：:Streptococcus agalactiae (group B Streptococcus [GBS]) is a cause of severe infections, particularly during the newborn period. While methods exist for generating chromosomal mutations in GBS, they are cumbersome and inefficient and present significant challenges if the goal is to study subtle mutations, such as singl...

journal_title：Applied and environmental microbiology

authors： Hooven TA,Bonakdar M,Chamby AB,Ratner AJ

更新日期：2019-03-22 00:00:00

abstract：:Bacterium T-52, cultured on ethylene glycol, readily oxidized glycolate and glyoxylate and exhibited elevated activities of ethylene glycol dehydrogenase and glycolate oxidase. Labeled glyoxylate was identified in reaction mixtures containing [C]-ethylene glycol, but no glycolate was detected. The most likely pathway ...

journal_title：Applied and environmental microbiology

authors： Caskey WH,Taber WA

更新日期：1981-07-01 00:00:00

abstract：:The bacteriocin nisin is produced only by some strains of Lactococcus lactis, and to date production in other lactic acid bacteria has not been achieved. Enterococcus sp. strain N12beta is a nisin-immune transconjugant obtained from a nisin-producing donor (L. lactis ATCC 11454) and a dairy recipient (Enterococcus sp....

journal_title：Applied and environmental microbiology

authors： Li H,O'Sullivan DJ

更新日期：2002-07-01 00:00:00

abstract：:A polyphasic approach involving cultivation, direct viable counts, rRNA-based phylogenetic classification, and in situ probing was applied for the characterization of the dominant microbial population in a municipal drinking water distribution system. A total of 234 bacterial strains cultivated on R2A medium were scre...

journal_title：Applied and environmental microbiology

authors： Kalmbach S,Manz W,Szewzyk U

更新日期：1997-11-01 00:00:00

abstract：:Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, an...

journal_title：Applied and environmental microbiology

authors： Petersen JM,Schriefer ME,Gage KL,Montenieri JA,Carter LG,Stanley M,Chu MC

更新日期：2004-06-01 00:00:00

abstract：:Yeasts able to grow on d-xylose were screened for the ability to hydrolyze xylan. Xylanase activity was found to be rare; a total of only 19 of more than 250 strains yielded a positive test result. The activity was localized largely in the genus Cryptococcus and in Pichia stipitis and its anamorph Candida shehatae. Th...

journal_title：Applied and environmental microbiology

authors： Lee H,Biely P,Latta RK,Barbosa MF,Schneider H

更新日期：1986-08-01 00:00:00

abstract：:"Dehalococcoides ethenogenes" 195 can reductively dechlorinate tetrachloroethene (PCE) completely to ethene (ETH). When PCE-grown strain 195 was transferred (2% [vol/vol] inoculum) into growth medium amended with trichloroethene (TCE), cis-dichloroethene (DCE), 1,1-DCE, or 1,2-dichloroethane (DCA) as an electron accep...

journal_title：Applied and environmental microbiology

authors： Maymó-Gatell X,Anguish T,Zinder SH

更新日期：1999-07-01 00:00:00

abstract：:The differential resistance of substratum-attached, detached, and planktonic cells of Salmonella enteritidis phage type 4 was studied by using several inimical processes and in vivo bioluminescence as a nondestructive, real-time reporter of metabolic activity. Bioluminescence in this strain was mediated by a construct...

journal_title：Applied and environmental microbiology

authors： Dhir VK,Dodd CE

更新日期：1995-05-01 00:00:00

abstract：:Symbiotic microorganisms have been found in the hemolymph (blood) of many aquatic invertebrates, such as crabs, shrimp, and oysters. Hemolymph is a critical site in the host immune response. Currently, studies on hemolymph microorganisms are mostly performed with culture-dependent strategies using selective media (e.g...

journal_title：Applied and environmental microbiology

authors： Zhang X,Sun Z,Zhang X,Zhang M,Li S

更新日期：2018-04-02 00:00:00