Generation of amylosucrase variants that terminate catalysis of acceptor elongation at the di- or trisaccharide stage.

abstract：:A clinical isolate of Pseudomonas aeruginosa was found capable of utilizing salicylate by the salicylate hydroxylase and beta-ketoadipate pathway. ...

journal_title：Applied and environmental microbiology

authors： Ohta S,Matsumoto H,Terawaki Y

更新日期：1981-01-01 00:00:00

abstract：:PCR generated two distinct products from a toxic isolate of Alexandrium catenella, which had been taken from Dai Ya Bay (southern China), by using primers for large-subunit rRNA. This pattern is distinct from published data for North American Alexandrium species. Sequences of the two products suggest that the smaller ...

journal_title：Applied and environmental microbiology

authors： Yeung PK,Kong KF,Wong FT,Wong JT

更新日期：1996-11-01 00:00:00

abstract：:Recent declines in sea grass distribution underscore the importance of understanding microbial community structure-function relationships in sea grass rhizospheres that might affect the viability of these plants. Phospholipid fatty acid analyses showed that sulfate-reducing bacteria and clostridia were enriched in sed...

journal_title：Applied and environmental microbiology

authors： Küsel K,Pinkart HC,Drake HL,Devereux R

更新日期：1999-11-01 00:00:00

abstract：:Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhi...

journal_title：Applied and environmental microbiology

authors： Fan Z,Wagschal K,Chen W,Montross MD,Lee CC,Yuan L

更新日期：2009-03-01 00:00:00

abstract：:Free-living soybean rhizobia and Bradyrhizobium spp. (lupine) have the ability to catabolize ethanol. Of the 30 strains of rhizobia examined, only the fast- and slow-growing soybean rhizobia and the slow-growing Bradyrhizobium sp. (lupine) were capable of using ethanol as a sole source of carbon and energy for growth....

journal_title：Applied and environmental microbiology

authors： Sadowsky MJ,Bohlool BB

更新日期：1986-10-01 00:00:00

abstract：:DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent...

journal_title：Applied and environmental microbiology

authors： Lovell CR,Friez MJ,Longshore JW,Bagwell CE

更新日期：2001-11-01 00:00:00

abstract：:A repeated cross-sectional study was conducted to identify farm management, environment, weather, and landscape factors that predict the count of generic Escherichia coli on spinach at the preharvest level. E. coli was enumerated for 955 spinach samples collected on 12 farms in Texas and Colorado between 2010 and 2012...

journal_title：Applied and environmental microbiology

authors： Park S,Navratil S,Gregory A,Bauer A,Srinath I,Szonyi B,Nightingale K,Anciso J,Jun M,Han D,Lawhon S,Ivanek R

更新日期：2015-04-01 00:00:00

abstract：:Transfer of plasmid-borne antibiotic resistance genes in Escherichia coli wild-type strains is possible by transformation under naturally occurring conditions in oligotrophic, aquatic environments containing physiologic concentrations of calcium. In contrast, transformation is suppressed in nitrogen-rich body fluids l...

journal_title：Applied and environmental microbiology

authors： Woegerbauer M,Jenni B,Thalhammer F,Graninger W,Burgmann H

更新日期：2002-01-01 00:00:00

abstract：:Cells of a newly isolated environmental strain of Candida humicola accumulated 10-fold more polyphosphate (polyP), during active growth, when grown in complete glucose-mineral salts medium at pH 5.5 than when grown at pH 7.5. Neither phosphate starvation, nutrient limitation, nor anaerobiosis was required to induce po...

journal_title：Applied and environmental microbiology

authors： McGrath JW,Quinn JP

更新日期：2000-09-01 00:00:00

abstract：:The existence of airborne mycotoxins in mold-contaminated buildings has long been hypothesized to be a potential occupant health risk. However, little work has been done to demonstrate the presence of these compounds in such environments. The presence of airborne macrocyclic trichothecene mycotoxins in indoor environm...

journal_title：Applied and environmental microbiology

authors： Brasel TL,Martin JM,Carriker CG,Wilson SC,Straus DC

更新日期：2005-11-01 00:00:00

abstract：:Flocs consisting of Anabaena and Zoogloea spp. were used as a model system for the study of planktonic phototroph-heterotroph interactions. In CO(2)-limited continuous culture (3.2 mumol of NaHCO(3) liter h, 1.5 mumol of glucose liter h, pH 8.5, D = 0.026 h), the biomass of the phototroph increased 8.6-fold due to ass...

journal_title：Applied and environmental microbiology

authors： Schiefer GE,Caldwell DE

更新日期：1982-07-01 00:00:00

abstract：:Effects of the sesquiterpene farnesol (3,7,11-trimethyl-2,6,10-dodecatrien-1-ol) and the sesquiterpene derivative geosmin (1,10-trans-dimethyl-trans-9-decalol) were investigated in a geosmin-producing actinomycete, Streptomyces tendae. Exposure to 300 microM farnesol reduced biomass (fresh matter) accumulation by 97% ...

journal_title：Applied and environmental microbiology

authors： Dionigi CP,Millie DF,Johnsen PB

更新日期：1991-12-01 00:00:00

abstract：:Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

journal_title：Applied and environmental microbiology

authors： Ramírez-Reinat EL,Garcia-Pichel F

更新日期：2012-01-01 00:00:00

abstract：:Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. ...

journal_title：Applied and environmental microbiology

authors： King AD Jr,Hocking AD,Pitt JI

更新日期：1979-05-01 00:00:00

abstract：:Some species of ruminal bacteria are known to produce antimicrobial peptides, but the screening procedures have mostly been based on in vitro assays using standardized methods. Recent sequencing efforts have made available the genome sequences of hundreds of ruminal microorganisms. In this work, we performed genome mi...

journal_title：Applied and environmental microbiology

authors： Azevedo AC,Bento CB,Ruiz JC,Queiroz MV,Mantovani HC

更新日期：2015-10-01 00:00:00

abstract：:Salmonella enterica serovars Typhimurium (S. Typhimurium) and Enteritidis (S. Enteritidis) are foodborne pathogens, and outbreaks are often associated with poultry products. Chickens are typically asymptomatic when colonized by these serovars; however, the factors contributing to this observation are uncharacterized. ...

journal_title：Applied and environmental microbiology

authors： Troxell B,Petri N,Daron C,Pereira R,Mendoza M,Hassan HM,Koci MD

更新日期：2015-12-01 00:00:00

abstract：:Four sourdoughs (A to D) were produced under practical conditions by using a starter mixture of three commercially available sourdough starters and a baker's yeast constitutively containing various species of lactic acid bacteria (LAB). The sourdoughs were continuously propagated until the composition of the LAB flora...

journal_title：Applied and environmental microbiology

authors： Meroth CB,Walter J,Hertel C,Brandt MJ,Hammes WP

更新日期：2003-01-01 00:00:00

abstract：:A high biomasses of Cladophora, a filamentous green alga, is found mainly during the summer along the shores of Lake Michigan. In this study, the abundance and persistence of the fecal indicator bacterium Escherichia coli and sulfate-reducing bacteria (SRB) on Cladophora mats collected at Lake Michigan beaches were ev...

journal_title：Applied and environmental microbiology

authors： Olapade OA,Depas MM,Jensen ET,McLellan SL

更新日期：2006-03-01 00:00:00

abstract：:Adsorption of Agrobacterium tumefaciens cells to potato tuber disks reached equilibrium after coincubation for about 30 min. More than 10% of the number of bacteria bound at equilibrium were adsorbed within 30 s. Adsorption isotherms obtained at three temperatures showed that the heat of adsorption was nearly zero. ...

journal_title：Applied and environmental microbiology

authors： Kluepfel DA,Pueppke SG

更新日期：1986-05-01 00:00:00

abstract：:An accurate most-probable-number enumeration method was developed for counting the number of Beggiatoa trichomes from various freshwater sediments. The medium consisted of extracted hay, diluted soil extract, 0.05% acetate, and 15 to 35 U of catalase per ml. The same enrichment medium, but without the acetate, was the...

journal_title：Applied and environmental microbiology

authors： Strohl WR,Larkin JM

更新日期：1978-11-01 00:00:00

abstract：:Enzymatic digestion in vitro of the Bacillus thuringiensis protoxin presumably releases and activates the toxin in a manner analogous to that which occurs when a B. thuringiensis sporulated fermentation preparation passes through the midgut of a lepidopteran larva. Therefore, a sporulated culture of B. thuringiensis s...

journal_title：Applied and environmental microbiology

authors： Aronson JN,Arvidson HC

更新日期：1987-02-01 00:00:00

abstract：:A novel flagellatropic phage of Salmonella enterica serovar Typhimurium, called iEPS5, was isolated and characterized. iEPS5 has an icosahedral head and a long noncontractile tail with a tail fiber. Genome sequencing revealed a double-stranded DNA of 59,254 bp having 73 open reading frames (ORFs). To identify the rece...

journal_title：Applied and environmental microbiology

authors： Choi Y,Shin H,Lee JH,Ryu S

更新日期：2013-08-01 00:00:00

abstract：:7 alpha-Dehydroxylation of cholic acid and chenodeoxycholic acid by whole cells of strain c-25, a Eubacterium lentum-like intestinal anaerobe, was studied. 7 alpha-Dehydroxylase activity was observed only in whole cells grown in the presence of the primary bile acid (cholic acid or chenodeoxycholic acid). Chenodeoxych...

journal_title：Applied and environmental microbiology

authors： Masuda N,Oda H,Hirano S,Masuda M,Tanaka H

更新日期：1984-04-01 00:00:00

abstract：:A gene coding for an aminopeptidase (PepC) from Lactococcus lactis subsp. cremoris AM2 was cloned by complementation of an Escherichia coli mutant lacking aminopeptidase activity. The nucleotide sequence was determined. A portion of the predicted amino acid sequence of PepC (436 amino acids) showed strong homology to ...

journal_title：Applied and environmental microbiology

authors： Chapot-Chartier MP,Nardi M,Chopin MC,Chopin A,Gripon JC

更新日期：1993-01-01 00:00:00

abstract：:Salmonella enterica contamination in foods is a significant concern for public health. When DNA detection methods are used for analysis of foods, one of the major concerns is false-positive results from the detection of dead cells. To circumvent this crucial issue, a TaqMan quantitative real-time RT-PCR (qRT-PCR) assa...

journal_title：Applied and environmental microbiology

authors： González-Escalona N,Hammack TS,Russell M,Jacobson AP,De Jesús AJ,Brown EW,Lampel KA

更新日期：2009-06-01 00:00:00

abstract：:The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficienc...

journal_title：Applied and environmental microbiology

authors： Eom GT,Song JK,Ahn JH,Seo YS,Rhee JS

更新日期：2005-07-01 00:00:00

abstract：:The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring t...

journal_title：Applied and environmental microbiology

authors： Li J,Hu X,Yan J,Yuan Z

更新日期：2009-06-01 00:00:00

abstract：:Thirty-nine isolates of fungi obtained from foodstuffs and soil samples from various parts of the world have been identified. The isolates were grown on a solid rice medium, and extracts were prepared with 50% aqueous methanol. The extracts were examined for toxicity in the following systems: (i) cytotoxicity to cultu...

journal_title：Applied and environmental microbiology

authors： Abbas HK,Mirocha CJ,Shier WT

更新日期：1984-09-01 00:00:00

abstract：:Two methods, digestion and elution, were used to recover parasitic nematodes from 470 flatfish belonging to species in the family Pleuronectidae. Samples of similar fish were collected from market lots; half of each sample was subjected to digestion, and half was subjected to elution (sedimentation). The edible (flesh...

journal_title：Applied and environmental microbiology

authors： Jackson GJ,Bier JW,Payne WL,McClure FD

更新日期：1981-04-01 00:00:00

abstract：:Endotoxin exposure occurs in homes and occupational environments and is known to cause adverse health effects. In order to compare results from different studies and establish standards, airborne endotoxin exposures should be assessed using standardized methods. Although the European Committee for Standardization (CEN...

journal_title：Applied and environmental microbiology

authors： Spaan S,Heederik DJ,Thorne PS,Wouters IM

更新日期：2007-10-01 00:00:00