Enhancement of the efficiency of secretion of heterologous lipase in Escherichia coli by directed evolution of the ABC transporter system.

abstract：:Diversity among 130 strains of Bacillus polymyxa was studied; the bacteria were isolated by immunotrapping from nonrhizosphere soil (32 strains), rhizosphere soil (38 strains), and the rhizoplane (60 strains) of wheat plantlets growing in a growth chamber. The strains were characterized phenotypically by 63 auxanograp...

journal_title：Applied and environmental microbiology

authors： Mavingui P,Laguerre G,Berge O,Heulin T

更新日期：1992-06-01 00:00:00

abstract：:The effect of the glutathione reductase (GshR) activity of Lactobacillus sanfranciscensis DSM20451(T) on the thiol levels in fermented sourdoughs was determined, and the oxygen tolerance of the strain was also determined. The gshR gene coding for a putative GshR was sequenced and inactivated by single-crossover integr...

journal_title：Applied and environmental microbiology

authors： Jänsch A,Korakli M,Vogel RF,Gänzle MG

更新日期：2007-07-01 00:00:00

abstract：:Examination of cucumber roots (Cucumis sativus L.) grown in bark compost media and of the surrounding edaphic substrate showed profiles of polar lipid fatty acids commonly found in bacteria. The composition of fatty acids in these profiles differed significantly between roots grown in a medium naturally suppressive to...

journal_title：Applied and environmental microbiology

authors： Tunlid A,Hoitink HA,Low C,White DC

更新日期：1989-06-01 00:00:00

abstract：:The fate of 14C-labeled 2,4,6-trinitrotoluene (TNT) in an activated-sludge system was investigated. No [14C]TNT could be detected in the contents of an aerated reactor after 3 to 5 days of incubation. No significant 14CO2 was formed, and the radioactivity was about equally divided between the floc and the supernatant....

journal_title：Applied and environmental microbiology

authors： Carpenter DF,McCormick NG,Cornell JH,Kaplan AM

更新日期：1978-05-01 00:00:00

abstract：:In this article, a new mechanism influencing the transport of microorganisms through unsaturated porous media is examined, and a new method for directly visualizing bacterial behavior within a porous medium under controlled chemical and flow conditions is introduced. Resting cells of hydrophilic and relatively hydroph...

journal_title：Applied and environmental microbiology

authors： Wan J,Wilson JL,Kieft TL

更新日期：1994-02-01 00:00:00

abstract：:The production of pectolytic enzymes (pectate lyase [Pel] and polygalacturonase [Peh]), cellulase (Cel), and protease (Prt) is activated in the soft rot bacterium Erwinia carotovora subsp. carotovora by aepA (activator of extracellular protein production) and celery extract (Y. Liu, H. Murata, A. Chatterjee, and A. K....

journal_title：Applied and environmental microbiology

authors： Murata H,Chatterjee A,Liu Y,Chatterjee AK

更新日期：1994-09-01 00:00:00

abstract：:Previous studies have demonstrated that reduced humic substances (HS) can be reoxidized by anaerobic bacteria such as Geobacter, Geothrix, and Wolinella species with a suitable electron acceptor; however, little is known of the importance of this metabolism in the environment. Recently we investigated this metabolism ...

journal_title：Applied and environmental microbiology

authors： Coates JD,Cole KA,Chakraborty R,O'Connor SM,Achenbach LA

更新日期：2002-05-01 00:00:00

abstract：:Some nitrate- and Fe(III)-reducing microorganisms are capable of oxidizing Fe(II) with nitrate as the electron acceptor. This enzymatic pathway may facilitate the development of anaerobic microbial communities that take advantage of the energy available during Fe-N redox oscillations. We examined this phenomenon in sy...

journal_title：Applied and environmental microbiology

authors： Coby AJ,Picardal F,Shelobolina E,Xu H,Roden EE

更新日期：2011-09-01 00:00:00

abstract：:Trichoderma reesei can produce up to 100 g/liter of extracellular proteins. The major and industrially relevant products are cellobiohydrolase I (CBHI) and the hemicellulase XYNI. The genes encoding both enzymes are transcriptionally activated by the regulatory protein Xyr1. The first 850 nucleotides of the cbh1 promo...

journal_title：Applied and environmental microbiology

authors： Kiesenhofer DP,Mach RL,Mach-Aigner AR

更新日期：2017-12-15 00:00:00

abstract：:The ferric uptake regulator (Fur) box-like sequence was located upstream of the serine protease-encoding gene (aprII) from a marine bacterium, Alteromonas sp. strain O-7. To clarify whether the production of AprII (the gene product of aprII) is regulated by the environmental iron concentrations, this strain was cultur...

journal_title：Applied and environmental microbiology

authors： Tsujibo H,Miyamoto K,Okamoto T,Orikoshi H,Inamori Y

更新日期：2000-09-01 00:00:00

abstract：:A comprehensive assessment of bacterial diversity and community composition in arctic and antarctic pack ice was conducted through cultivation and cultivation-independent molecular techniques. We sequenced 16S rRNA genes from 115 and 87 pure cultures of bacteria isolated from arctic and antarctic pack ice, respectivel...

journal_title：Applied and environmental microbiology

authors： Brinkmeyer R,Knittel K,Jürgens J,Weyland H,Amann R,Helmke E

更新日期：2003-11-01 00:00:00

abstract：:The milk supply is considered a primary route for a bioterrorism attack with Bacillus anthracis spores because typical high-temperature short-time (HTST) pasteurization conditions cannot inactivate spores. In the event of intentional contamination, an effective method to inactivate the spores in milk under HTST proces...

journal_title：Applied and environmental microbiology

authors： Xu S,Labuza TP,Diez-Gonzalez F

更新日期：2008-06-01 00:00:00

abstract：:The polymerase chain reaction (PCR) and a radiolabeled oligonucleotide probe were used to specifically detect proteolytic and nonproteolytic Clostridium botulinum type B. Two synthetic primers deduced from the amino acid sequence data of type B neurotoxin were used to amplify a 1.5-kbp fragment corresponding to the li...

journal_title：Applied and environmental microbiology

authors： Szabo EA,Pemberton JM,Desmarchelier PM

更新日期：1992-01-01 00:00:00

abstract：:In this study, the inactivating properties of liquid hydrogen peroxide (L-H(2)O(2)), vaporized hydrogen peroxide (V-H(2)O(2)), UV light, and a combination of V-H(2)O(2) and UV light were tested on murine norovirus 1 (MNV-1) and bacteriophages (φX174 and B40-8) as models for human noroviruses. Disinfection of surfaces ...

journal_title：Applied and environmental microbiology

authors： Li D,Baert L,De Jonghe M,Van Coillie E,Ryckeboer J,Devlieghere F,Uyttendaele M

更新日期：2011-02-01 00:00:00

abstract：:Group I strains of Clostridium botulinum are known to degrade arginine by the arginine deiminase pathway. We have found that C. botulinum Okra B consumed a level of arginine (20 g/liter) higher than the basal requirement for growth (3 g/liter). Arginine was probably the preferred source of nitrogen for biosynthesis bu...

journal_title：Applied and environmental microbiology

authors： Patterson-Curtis SI,Johnson EA

更新日期：1992-07-01 00:00:00

abstract：:Erwinia carotovora subsp. atroseptica, E. carotovora subsp. betavasculorum, and E. carotovora subsp. carotovora produce high levels of extracellular enzymes, such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt), and the quorum-sensing signal N-acyl-homoserine lactone (AHL) at 28 de...

journal_title：Applied and environmental microbiology

authors： Hasegawa H,Chatterjee A,Cui Y,Chatterjee AK

更新日期：2005-08-01 00:00:00

abstract：:A bioassay method was developed to measure the average intensity within a UV disinfection reactor. The survival of spores of Bacillus subtilis was determined as a function of UV dose to prepare a standard curve. Spores were added to unknown systems, and the survival rate was used to determine the average intensity. A ...

journal_title：Applied and environmental microbiology

authors： Qualls RG,Johnson JD

更新日期：1983-03-01 00:00:00

abstract：:The so-called Fe/Mn-oxidizing bacteria have long been recognized for their potential to form extracellular iron hydroxide or manganese oxide structures in aquatic environments. Bacterial species belonging to the genus Gallionella, one type of such bacteria, oxidize iron and produce uniquely twisted extracellular stalk...

journal_title：Applied and environmental microbiology

authors： Suzuki T,Hashimoto H,Matsumoto N,Furutani M,Kunoh H,Takada J

更新日期：2011-05-01 00:00:00

abstract：:Simulation of a heat process used in the terminal dry-heat decontamination of the Viking spacecraft is reported. Naturally occurring airborne bacterial spores were collected on Teflon ribbons in selected spacecraft assembly areas and subsequently subjected to dry heat. Thermal inactivation experiments were conducted a...

journal_title：Applied and environmental microbiology

authors： Puleo JR,Bergstrom SL,Peeler JT,Oxborrow GS

更新日期：1978-09-01 00:00:00

abstract：:Enteric microbiota play a variety of roles in intestinal health and disease. While bacteria in the intestine have been broadly characterized, little is known about the abundance or diversity of enteric fungi. This study utilized a culture-independent method termed oligonucleotide fingerprinting of rRNA genes (OFRG) to...

journal_title：Applied and environmental microbiology

authors： Scupham AJ,Presley LL,Wei B,Bent E,Griffith N,McPherson M,Zhu F,Oluwadara O,Rao N,Braun J,Borneman J

更新日期：2006-01-01 00:00:00

abstract：:Pseudomonas oleovorans grows on C(6) to C(12)n-alkanes and 1-alkenes. These substrates are oxidized to the corresponding fatty acids, which are oxidized further via the beta-oxidation pathway, yielding shorter fatty acids which have lost one or more C(2) units. P. oleovorans normally utilizes beta-oxidation pathway in...

journal_title：Applied and environmental microbiology

authors： Lageveen RG,Huisman GW,Preusting H,Ketelaar P,Eggink G,Witholt B

更新日期：1988-12-01 00:00:00

abstract：:Pyocins are bacteriocins secreted by Pseudomonas aeruginosa, and they assist in the colonization of different niches. A major subset of these antibacterial proteins adopt a modular organization characteristic of polymorphic toxins. They include a receptor-binding domain, a segment enabling membrane passage, and a toxi...

journal_title：Applied and environmental microbiology

authors： Ghequire MGK,Öztürk B

更新日期：2018-08-31 00:00:00

abstract：:Rhizoremediation of organic chemicals requires high-level expression of biodegradation genes in bacterial strains that are excellent rhizosphere colonizers. Pseudomonas fluorescens F113 is a biocontrol strain that was shown to be an excellent colonizer of numerous plant rhizospheres, including alfalfa. Although a deri...

journal_title：Applied and environmental microbiology

authors： Villacieros M,Whelan C,Mackova M,Molgaard J,Sánchez-Contreras M,Lloret J,Aguirre de Cárcer D,Oruezábal RI,Bolaños L,Macek T,Karlson U,Dowling DN,Martín M,Rivilla R

更新日期：2005-05-01 00:00:00

abstract：:Vibrio cholerae can shift to a "rugose" phenotype, thereby producing copious exopolysaccharide (EPS), which promotes its environmental survival and persistence. We report conditions that promote high-frequency rugose EPS production (HFRP), whereby cells switch at high frequency (up to 80%) to rugose EPS production. HF...

journal_title：Applied and environmental microbiology

authors： Ali A,Rashid MH,Karaolis DK

更新日期：2002-11-01 00:00:00

abstract：:Strains of Xylella fastidiosa isolated from grape, almond, maple, and oleander were characterized by enterobacterial repetitive intergenic consensus sequence-, repetitive extragenic palindromic element (REP)-, and random amplified polymorphic DNA (RAPD)-PCR; contour-clamped homogeneous electric field (CHEF) gel electr...

journal_title：Applied and environmental microbiology

authors： Hendson M,Purcell AH,Chen D,Smart C,Guilhabert M,Kirkpatrick B

更新日期：2001-02-01 00:00:00

abstract：:In various sediments in which Fe(III) reduction was the terminal electron-accepting process, [C]glucose was fermented to C-fatty acids in a manner similar to that observed in methanogenic sediments. These results are consistent with the hypothesis that, in Fe(III)-reducing sediments, fermentable substrates are oxidize...

journal_title：Applied and environmental microbiology

authors： Lovley DR,Phillips EJ

更新日期：1989-12-01 00:00:00

abstract：:A database was probed with artificial neural network (ANN) and multivariate logistic regression (MLR) models to investigate the efficacy of predicting PCR-identified human adenovirus (ADV), Norwalk-like virus (NLV), and enterovirus (EV) presence or absence in shellfish harvested from diverse countries in Europe (Spain...

journal_title：Applied and environmental microbiology

authors： Brion G,Viswanathan C,Neelakantan TR,Lingireddy S,Girones R,Lees D,Allard A,Vantarakis A

更新日期：2005-09-01 00:00:00

abstract：:Oil field bacteria were characterized by cloning and sequencing of PCR-amplified 16S rRNA genes. A variety of gram-negative, sulfate-reducing bacteria was detected (16 members of the family Desulfovibrionaceae and 8 members of the family Desulfobacteriaceae). In contrast, a much more limited number of anaerobic, ferme...

journal_title：Applied and environmental microbiology

authors： Voordouw G,Armstrong SM,Reimer MF,Fouts B,Telang AJ,Shen Y,Gevertz D

更新日期：1996-05-01 00:00:00

abstract：:The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

journal_title：Applied and environmental microbiology

authors： Schendel FJ,Flickinger MC

更新日期：1992-09-01 00:00:00

abstract：:Aerobic activated sludge granules are dense, spherical biofilms which can strongly improve purification efficiency and sludge settling in wastewater treatment processes. In this study, the structure and development of different granule types were analyzed. Biofilm samples originated from lab-scale sequencing batch rea...

journal_title：Applied and environmental microbiology

authors： Weber SD,Ludwig W,Schleifer KH,Fried J

更新日期：2007-10-01 00:00:00