Abstract:
:In this article, a new mechanism influencing the transport of microorganisms through unsaturated porous media is examined, and a new method for directly visualizing bacterial behavior within a porous medium under controlled chemical and flow conditions is introduced. Resting cells of hydrophilic and relatively hydrophobic bacterial strains isolated from groundwater were used as model microorganisms. The degree of hydrophobicity was determined by contact-angle measurements. Glass micromodels allowed the direct observation of bacterial behavior on a pore scale, and three types of sand columns with different gas saturations provided quantitative measurements of the observed phenomena on a porous medium scale. The reproducibility of each break-through curve was established in three to five repeated experiments. The data collected from the column experiments can be explained by phenomena directly observed in the micromodel experiments. The retention rate of bacteria is proportional to the gas saturation in porous media because of the preferential sorption of bacteria onto the gas-water interface over the solid-water interface. The degree of sorption is controlled mainly by cell surface hydrophobicity under the simulated groundwater conditions because of hydrophobic forces between the organisms and the interfaces. The sorption onto the gas-water interface is essentially irreversible because of capillary forces. This preferential and irreversible sorption at the gas-water interface strongly influences the movement and spatial distribution of microorganisms.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Wan J,Wilson JL,Kieft TLdoi
10.1128/AEM.60.2.509-516.1994keywords:
subject
Has Abstractpub_date
1994-02-01 00:00:00pages
509-16issue
2eissn
0099-2240issn
1098-5336journal_volume
60pub_type
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更新日期:1984-01-01 00:00:00
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更新日期:1995-12-01 00:00:00
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更新日期:1994-08-01 00:00:00
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更新日期:1979-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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