Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp.

Abstract:

:The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP with stoichiometric release of nitrite. During induction of the enzymes required for growth on PNP, 1,2,4-benzenetriol was identified as an intermediate by gas chromatography-mass spectroscopy (GC-MS) and radiotracer studies. 1,2,4-Benzenetriol was converted to maleylacetic acid, which was further degraded by the beta-ketoadipate pathway. Conversion of PNP to 1,2,4-benzenetriol is catalyzed by a monooxygenase system in strain JS443 through the formation of 4-nitrocatechol, 4-nitroresorcinol, or both. Our results clearly indicate the existence of an alternative pathway for the biodegradation of PNP.

journal_name

Appl Environ Microbiol

authors

Jain RK,Dreisbach JH,Spain JC

doi

10.1128/AEM.60.8.3030-3032.1994

subject

Has Abstract

pub_date

1994-08-01 00:00:00

pages

3030-2

issue

8

eissn

0099-2240

issn

1098-5336

journal_volume

60

pub_type

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