Genotypic characterization of Bradyrhizobium strains nodulating endemic woody legumes of the Canary Islands by PCR-restriction fragment length polymorphism analysis of genes encoding 16S rRNA (16S rDNA) and 16S-23S rDNA intergenic spacers, repetitive extr

Abstract:

:We present a phylogenetic analysis of nine strains of symbiotic nitrogen-fixing bacteria isolated from nodules of tagasaste (Chamaecytisus proliferus) and other endemic woody legumes of the Canary Islands, Spain. These and several reference strains were characterized genotypically at different levels of taxonomic resolution by computer-assisted analysis of 16S ribosomal DNA (rDNA) PCR-restriction fragment length polymorphisms (PCR-RFLPs), 16S-23S rDNA intergenic spacer (IGS) RFLPs, and repetitive extragenic palindromic PCR (rep-PCR) genomic fingerprints with BOX, ERIC, and REP primers. Cluster analysis of 16S rDNA restriction patterns with four tetrameric endonucleases grouped the Canarian isolates with the two reference strains, Bradyrhizobium japonicum USDA 110spc4 and Bradyrhizobium sp. strain (Centrosema) CIAT 3101, resolving three genotypes within these bradyrhizobia. In the analysis of IGS RFLPs with three enzymes, six groups were found, whereas rep-PCR fingerprinting revealed an even greater genotypic diversity, with only two of the Canarian strains having similar fingerprints. Furthermore, we show that IGS RFLPs and even very dissimilar rep-PCR fingerprints can be clustered into phylogenetically sound groupings by combining them with 16S rDNA RFLPs in computer-assisted cluster analysis of electrophoretic patterns. The DNA sequence analysis of a highly variable 264-bp segment of the 16S rRNA genes of these strains was found to be consistent with the fingerprint-based classification. Three different DNA sequences were obtained, one of which was not previously described, and all belonged to the B. japonicum/Rhodopseudomonas rDNA cluster. Nodulation assays revealed that none of the Canarian isolates nodulated Glycine max or Leucaena leucocephala, but all nodulated Acacia pendula, C. proliferus, Macroptilium atropurpureum, and Vigna unguiculata.

journal_name

Appl Environ Microbiol

authors

Vinuesa P,Rademaker JL,de Bruijn FJ,Werner D

doi

10.1128/AEM.64.6.2096-2104.1998

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

2096-104

issue

6

eissn

0099-2240

issn

1098-5336

journal_volume

64

pub_type

杂志文章
  • Inactivation of biofilm bacteria.

    abstract::The current project was developed to examine inactivation of biofilm bacteria and to characterize the interaction of biocides with pipe surfaces. Unattached bacteria were quite susceptible to the variety of disinfectants tested. Viable bacterial counts were reduced 99% by exposure to 0.08 mg of hypochlorous acid (pH 7...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.10.2492-2499.1988

    authors: LeChevallier MW,Cawthon CD,Lee RG

    更新日期:1988-10-01 00:00:00

  • Degradation pathway of bisphenol A: does ipso substitution apply to phenols containing a quaternary alpha-carbon structure in the para position?

    abstract::The degradation of bisphenol A and nonylphenol involves the unusual rearrangement of stable carbon-carbon bonds. Some nonylphenol isomers and bisphenol A possess a quaternary alpha-carbon atom as a common structural feature. The degradation of nonylphenol in Sphingomonas sp. strain TTNP3 occurs via a type II ipso subs...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00329-07

    authors: Kolvenbach B,Schlaich N,Raoui Z,Prell J,Zühlke S,Schäffer A,Guengerich FP,Corvini PF

    更新日期:2007-08-01 00:00:00

  • Increased production of folate by metabolic engineering of Lactococcus lactis.

    abstract::The dairy starter bacterium Lactococcus lactis is able to synthesize folate and accumulates large amounts of folate, predominantly in the polyglutamyl form. Only small amounts of the produced folate are released in the extracellular medium. Five genes involved in folate biosynthesis were identified in a folate gene cl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.6.3069-3076.2003

    authors: Sybesma W,Starrenburg M,Kleerebezem M,Mierau I,de Vos WM,Hugenholtz J

    更新日期:2003-06-01 00:00:00

  • Rate of Pyrite Bioleaching by Thiobacillus ferrooxidans: Results of an Interlaboratory Comparison.

    abstract::Ten laboratories participated in an interlaboratory comparison of determination of bioleaching rates of a pyrite reference material. A standardized procedure and a single strain of Thiobacillus ferrooxidans were used in this study. The mean rate of bioleaching of the pyrite reference material was 12.4 mg of Fe per lit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.3.642-644.1991

    authors: Olson GJ

    更新日期:1991-03-01 00:00:00

  • Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp. strain BAV1.

    abstract::Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.6347-6351.2004

    authors: Krajmalnik-Brown R,Hölscher T,Thomson IN,Saunders FM,Ritalahti KM,Löffler FE

    更新日期:2004-10-01 00:00:00

  • Sulfate-reducing bacteria in tubes constructed by the marine infaunal polychaete Diopatra cuprea.

    abstract::Marine infaunal burrows and tubes greatly enhance solute transport between sediments and the overlying water column and are sites of elevated microbial activity. Biotic and abiotic controls of the compositions and activities of burrow and tube microbial communities are poorly understood. The microbial communities in t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.12.7053-7065.2004

    authors: Matsui GY,Ringelberg DB,Lovell CR

    更新日期:2004-12-01 00:00:00

  • Effects of Swine manure on macrolide, lincosamide, and streptogramin B antimicrobial resistance in soils.

    abstract::Current agricultural practices involve inclusion of antimicrobials in animal feed and result in manure containing antimicrobials and antimicrobial-resistant microorganisms. This work evaluated the effects of land application of swine manure on the levels of tetracycline, macrolide, and lincosamide antimicrobials and o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02183-09

    authors: Zhou Z,Raskin L,Zilles JL

    更新日期:2010-04-01 00:00:00

  • Oral toxicity of Photorhabdus luminescens W14 toxin complexes in Escherichia coli.

    abstract::Previous attempts to express the toxin complex genes of Photorhabdus luminescens W14 in Escherichia coli have failed to reconstitute their oral toxicity to the model insect Manduca sexta. Here we show that the combination of three genes, tcdA, tcdB, and tccC, is essential for oral toxicity to M. sexta when expression ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.11.5017-5024.2001

    authors: Waterfield N,Dowling A,Sharma S,Daborn PJ,Potter U,Ffrench-Constant RH

    更新日期:2001-11-01 00:00:00

  • Evidence that elevated CO2 levels can indirectly increase rhizosphere denitrifier activity.

    abstract::We examined the influence of elevated CO2 concentration on denitrifier enzyme activity in wheat rhizoplanes by using controlled environments and solution culture techniques. Potential denitrification activity was from 3 to 24 times higher on roots that were grown under an elevated CO2 concentration of 1,000 micromoles...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.11.4621-4624.1997

    authors: Smart DR,Ritchie K,Stark JM,Bugbee B

    更新日期:1997-11-01 00:00:00

  • Tremorgenic mycotoxin from Penicillium paraherquei.

    abstract::A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen. It was produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium at 28 degrees C. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.3.441-442.1976

    authors: Yoshizawa T,Morooka N,Sawada Y,Udagawa SI

    更新日期:1976-09-01 00:00:00

  • Effect of lake trophic status and rooted macrophytes on community composition and abundance of ammonia-oxidizing prokaryotes in freshwater sediments.

    abstract::Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and acidic heathland...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02806-08

    authors: Herrmann M,Saunders AM,Schramm A

    更新日期:2009-05-01 00:00:00

  • Coliform aerosols generated from the surface of dewatered sewage applied to a forest clearcut.

    abstract::Concentrations of airborne coliform bacteria as high as 1.5 X 10(4) m-3 were observed 8 cm above anaerobically digested sewage sludge applied to a forest clearcut. Dry conditions and high wind speeds tended to favor aerosol generation. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.36.6.972-974.1978

    authors: Edmonds RL,Littke W

    更新日期:1978-12-01 00:00:00

  • Subsurface microbial methanotrophic mats in the Black Sea.

    abstract::A nodule-shaped microbial mat was found subsurface in sediments of a gas seep in the anoxic Black Sea. This mat was dominated by ANME-1 archaea and consumed methane and sulfate simultaneously. We propose that such subsurface mats represent the initial stage of previously investigated microbial reefs. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.10.6375-6378.2005

    authors: Treude T,Knittel K,Blumenberg M,Seifert R,Boetius A

    更新日期:2005-10-01 00:00:00

  • Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks.

    abstract::This report demonstrates the applicability of a combination of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and chemometrics for rapid and reliable identification of vegetative cells of the causative agent of anthrax, Bacillus anthracis. Bacillus cultures were prepared ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00857-09

    authors: Lasch P,Beyer W,Nattermann H,Stämmler M,Siegbrecht E,Grunow R,Naumann D

    更新日期:2009-11-01 00:00:00

  • Persistence and differential survival of fecal indicator bacteria in subtropical waters and sediments.

    abstract::Fecal coliforms and enterococci are indicator organisms used worldwide to monitor water quality. These bacteria are used in microbial source tracking (MST) studies, which attempt to assess the contribution of various host species to fecal pollution in water. Ideally, all strains of a given indicator organism (IO) woul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.6.3041-3048.2005

    authors: Anderson KL,Whitlock JE,Harwood VJ

    更新日期:2005-06-01 00:00:00

  • Production of Clostridium bifermentans Spores as Inoculum for Bioremediation of Nitroaromatic Contaminants.

    abstract::Spores of Clostridium bifermentans KMR-1 were produced for use as a microbial inoculum for bioremediation and were preserved in both liquid and dry forms. All spore formulations showed good viability and ability to biodegrade the target compound, 2,4,6-trinitrotoluene (TNT), after 4 months of storage. For low-cost bul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.5.2100-2104.1997

    authors: Sembries S,Crawford RL

    更新日期:1997-05-01 00:00:00

  • Bacterial endosymbiont localization in Hyalesthes obsoletus, the insect vector of Bois noir in Vitis vinifera.

    abstract::One emerging disease of grapevine in Europe is Bois noir (BN), a phytoplasmosis caused by "Candidatus Phytoplasma solani" and spread in vineyards by the planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae). Here we present the first full characterization of the bacterial community of this important disease vector co...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02121-10

    authors: Gonella E,Negri I,Marzorati M,Mandrioli M,Sacchi L,Pajoro M,Crotti E,Rizzi A,Clementi E,Tedeschi R,Bandi C,Alma A,Daffonchio D

    更新日期:2011-02-01 00:00:00

  • Nitrogen Fixation and Hydrogen Metabolism in Relation to the Dissolved Oxygen Tension in Chemostat Cultures of the Wild Type and a Hydrogenase-Negative Mutant of Azorhizobium caulinodans.

    abstract::Both the wild type and an isogenic hydrogenase-negative mutant of Azorhizobium caulinodans growing ex planta on N(2) as the N source were studied in succinate-limited steady-state chemostat cultures under 0.2 to 3.0% dissolved O(2) tension. Production or consumption of O(2), H(2), and CO(2) was measured with an on-lin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.6.1859-1866.1994

    authors: Boogerd FC,Ferdinandy-van Vlerken MM,Mawadza C,Pronk AF,Stouthamer AH,van Verseveld HW

    更新日期:1994-06-01 00:00:00

  • Influence of Polyethylene Glycol on the Size of Schizosaccharomyces pombe Electropores.

    abstract::The role of polyethylene glycol (PEG) in the transformation of Schizosaccharomyces pombe by electroporation is investigated by fluorescein isothiocyanate-dextran uptake and transformation studies. It is shown that when S. pombe cells are electroporated in the presence of PEG, the permeability state created is sustaine...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.4.1201-1206.1992

    authors: Hood MT,Stachow C

    更新日期:1992-04-01 00:00:00

  • Comparison of the seasonal variations of Synechococcus assemblage structures in estuarine waters and coastal waters of Hong Kong.

    abstract::Seasonal variation in the phylogenetic composition of Synechococcus assemblages in estuarine and coastal waters of Hong Kong was examined through pyrosequencing of the rpoC1 gene. Sixteen samples were collected in 2009 from two stations representing estuarine and ocean-influenced coastal waters, respectively. Synechoc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01895-15

    authors: Xia X,Vidyarathna NK,Palenik B,Lee P,Liu H

    更新日期:2015-11-01 00:00:00

  • Lag phase of Salmonella enterica under osmotic stress conditions.

    abstract::Salmonella enterica serovar Typhimurium was grown at salt concentrations ranging from 0.5 to 7.5% in minimal medium with and without added osmoprotectant and in a rich medium. In minimal medium, the cells showed an initial decline period, and consequently the definition of the lag time of the resultant log count curve...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02629-10

    authors: Zhou K,George SM,Métris A,Li PL,Baranyi J

    更新日期:2011-03-01 00:00:00

  • Raman spectroscopy-compatible inactivation method for pathogenic endospores.

    abstract::Micro-Raman spectroscopy is a fast and sensitive tool for the detection, classification, and identification of biological organisms. The vibrational spectrum inherently serves as a fingerprint of the biochemical composition of each bacterium and thus makes identification at the species level, or even the subspecies le...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02481-09

    authors: Stöckel S,Schumacher W,Meisel S,Elschner M,Rösch P,Popp J

    更新日期:2010-05-01 00:00:00

  • Leaf-cutter ant fungus gardens are biphasic mixed microbial bioreactors that convert plant biomass to polyols with biotechnological applications.

    abstract::Leaf-cutter ants use plant matter to culture the obligate mutualistic basidiomycete Leucoagaricus gongylophorus. This fungus mediates ant nutrition on plant resources. Furthermore, other microbes living in the fungus garden might also contribute to plant digestion. The fungus garden comprises a young sector with recen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00046-15

    authors: Somera AF,Lima AM,Dos Santos-Neto ÁJ,Lanças FM,Bacci M Jr

    更新日期:2015-07-01 00:00:00

  • Cloning, characterization, and functional expression of the Klebsiella oxytoca xylodextrin utilization operon (xynTB) in Escherichia coli.

    abstract::Escherichia coli is being developed as a biocatalyst for bulk chemical production from inexpensive carbohydrates derived from lignocellulose. Potential substrates include the soluble xylodextrins (xyloside, xylooligosaccharide) and xylobiose that are produced by treatments designed to expose cellulose for subsequent e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.10.5957-5967.2003

    authors: Qian Y,Yomano LP,Preston JF,Aldrich HC,Ingram LO

    更新日期:2003-10-01 00:00:00

  • Catabolism of tritiated thymidine by aquatic microbial communities and incorporation of tritium into RNA and protein.

    abstract::The incorporation of tritiated thymidine by five microbial ecosystems and the distribution of tritium into DNA, RNA, and protein were determined. All microbial assemblages tested exhibited significant labeling of RNA and protein (i.e., nonspecific labeling), as determined by differential acid-base hydrolysis. Nonspeci...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.5.1245-1254.1990

    authors: Brittain AM,Karl DM

    更新日期:1990-05-01 00:00:00

  • Psychrotolerant Paenibacillus tundrae isolates from barley grains produce new cereulide-like depsipeptides (paenilide and homopaenilide) that are highly toxic to mammalian cells.

    abstract::Paenilide is a novel, heat-stable peptide toxin from Paenibacillus tundrae, which colonizes barley. P. tundrae produced 20 to 50 ng of the toxin mg(-1) of cells (wet weight) throughout a range of growth temperatures from +5°C to +28°C. Paenilide consisted of two substances of 1,152 Da and 1,166 Da, with masses and tan...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00049-12

    authors: Rasimus S,Mikkola R,Andersson MA,Teplova VV,Venediktova N,Ek-Kommonen C,Salkinoja-Salonen M

    更新日期:2012-05-01 00:00:00

  • Gene expression induced in Escherichia coli O157:H7 upon exposure to model apple juice.

    abstract::Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription pattern...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02841-08

    authors: Bergholz TM,Vanaja SK,Whittam TS

    更新日期:2009-06-01 00:00:00

  • Antibacterial effects of grape extracts on Helicobacter pylori.

    abstract::Anti-Helicobacter pylori activities were determined by agar dilution, confocal laser scanning microscopy, and cell proliferation assays following treatment with various grape extracts. Muscadine grape skin possessed the strongest activity, followed by grape synergy (skin and seed) and seed, suggesting that higher phen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01595-08

    authors: Brown JC,Huang G,Haley-Zitlin V,Jiang X

    更新日期:2009-02-01 00:00:00

  • Effect of storage time and temperature on the survival of Clostridium botulinum spores in acid media.

    abstract::Clostridium-botulinum type A and type B spores were stored in tomato juice (pH 4.2) and citric acid-phosphate buffer (pH 4.2) at 4, 22, and 32 degrees C for 180 days. The spore count was determined at different intervals over the 180-day storage period. There was no significant decrease in the number of type A spores ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.1.30-33.1977

    authors: Odlaug TE,Pflug IJ

    更新日期:1977-07-01 00:00:00

  • Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.

    abstract::A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR prime...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4888-4892.2005

    authors: Lin H,Civerolo EL,Hu R,Barros S,Francis M,Walker MA

    更新日期:2005-08-01 00:00:00