Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.

Abstract:

:A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, distributed across the X. fastidiosa genome, clearly differentiated and clustered X. fastidiosa strains collected from grape, almond, citrus, and oleander. They are well suited for differentiating strains and studying X. fastidiosa epidemiology and population genetics.

journal_name

Appl Environ Microbiol

authors

Lin H,Civerolo EL,Hu R,Barros S,Francis M,Walker MA

doi

10.1128/AEM.71.8.4888-4892.2005

keywords:

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

4888-92

issue

8

eissn

0099-2240

issn

1098-5336

pii

71/8/4888

journal_volume

71

pub_type

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