Abstract:
:A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, distributed across the X. fastidiosa genome, clearly differentiated and clustered X. fastidiosa strains collected from grape, almond, citrus, and oleander. They are well suited for differentiating strains and studying X. fastidiosa epidemiology and population genetics.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Lin H,Civerolo EL,Hu R,Barros S,Francis M,Walker MAdoi
10.1128/AEM.71.8.4888-4892.2005keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
4888-92issue
8eissn
0099-2240issn
1098-5336pii
71/8/4888journal_volume
71pub_type
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