Engineering trehalose synthesis in Lactococcus lactis for improved stress tolerance.

abstract：:Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, an...

journal_title：Applied and environmental microbiology

authors： Petersen JM,Schriefer ME,Gage KL,Montenieri JA,Carter LG,Stanley M,Chu MC

更新日期：2004-06-01 00:00:00

abstract：:The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat...

journal_title：Applied and environmental microbiology

authors： Ranneklev SB,Bååth E

更新日期：2003-06-01 00:00:00

abstract：:In Pseudomonas sp. strain M114, the outer membrane receptor for ferric pseudobactin M114 was shown to transport ferric pseudobactins B10 and A225, in addition to its own. The gene encoding this receptor, which was previously cloned on pCUP3, was localized by Tn5 mutagenesis to a region comprising >1.6 kb of M114 DNA. ...

journal_title：Applied and environmental microbiology

authors： Morris J,O'sullivan DJ,Koster M,Leong J,Weisbeek PJ,O'gara F

更新日期：1992-02-01 00:00:00

abstract：:The existence of a hydrogen sulfide:ferric ion oxidoreductase, which catalyzes the oxidation of elemental sulfur with ferric ions as an electron acceptor to produce ferrous and sulfite ions, was assayed with washed intact cells and cell extracts of various kinds of iron-oxidizing bacteria, such as Thiobacillus ferroox...

journal_title：Applied and environmental microbiology

authors： Sugio T,White KJ,Shute E,Choate D,Blake RC

更新日期：1992-01-01 00:00:00

abstract：:Until recently, Amblyomma maculatum (the Gulf Coast tick) had garnered little attention compared to other species of human-biting ticks in the United States. A. maculatum is now recognized as the principal vector of Rickettsia parkeri, a pathogenic spotted fever group rickettsia (SFGR) that causes an eschar-associated...

journal_title：Applied and environmental microbiology

authors： Paddock CD,Fournier PE,Sumner JW,Goddard J,Elshenawy Y,Metcalfe MG,Loftis AD,Varela-Stokes A

更新日期：2010-05-01 00:00:00

abstract：:To elevate the production level of heterologous polyketide in Streptomyces venezuelae, an additional copy of the positive regulatory gene pikD was introduced into the pikromycin (Pik) polyketide synthase (PKS) deletion mutant of S. venezuelae ATCC 15439 expressing tylosin PKS genes. The resulting mutant strain showed ...

journal_title：Applied and environmental microbiology

authors： Jung WS,Jeong SJ,Park SR,Choi CY,Park BC,Park JW,Yoon YJ

更新日期：2008-04-01 00:00:00

abstract：:An L-glutamine-overproducing mutant of an Escherichia coli K-12-derived strain was selected from randomly mutagenized cells in the course of L-alanyl-L-glutamine strain development. Genome-wide mutation analysis unveiled a novel mechanism for L-glutamine overproduction in this mutant. Three mutations were identified t...

journal_title：Applied and environmental microbiology

authors： Hayashi M,Tabata K

更新日期：2013-05-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli (EHEC) is a significant zoonotic pathogen causing severe disease associated with watery and bloody diarrhea, hemorrhagic colitis, and the hemolytic-uremic syndrome (HUS) in humans. Infections are frequently associated with contact with EHEC-contaminated ruminant feces. Both natural a...

journal_title：Applied and environmental microbiology

authors： Joris MA,Vanrompay D,Verstraete K,De Reu K,De Zutter L,Cox E

更新日期：2013-06-01 00:00:00

abstract：:DNA and peptide nucleic acid (PNA) molecular beacons were successfully used to detect rRNA in solution. In addition, PNA molecular beacon hybridizations were found to be useful for the quantification of rRNA: hybridization signals increased in a linear fashion with the 16S rRNA concentrations used in this experiment (...

journal_title：Applied and environmental microbiology

authors： Xi C,Balberg M,Boppart SA,Raskin L

更新日期：2003-09-01 00:00:00

abstract：:Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We pr...

journal_title：Applied and environmental microbiology

authors： Hashimoto Si,Katsumata R

更新日期：1999-06-01 00:00:00

abstract：:The variation in manure-amended soil survival capability among 18 Escherichia coli O157 strains (8 animal, 1 food, and 9 human isolates) was studied using a single sandy soil sample and a single sample of cattle manure as the inoculum carrier. The virulence profiles of E. coli O157 strains were characterized by detect...

journal_title：Applied and environmental microbiology

authors： Franz E,van Hoek AH,Bouw E,Aarts HJ

更新日期：2011-11-01 00:00:00

abstract：:In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...

journal_title：Applied and environmental microbiology

authors： Huang J,Sonnleitner E,Ren B,Xu Y,Haas D

更新日期：2012-07-01 00:00:00

abstract：:This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology. A central composite design was used as an experimental design ...

journal_title：Applied and environmental microbiology

authors： Oh S,Rheem S,Sim J,Kim S,Baek Y

更新日期：1995-11-01 00:00:00

abstract：:Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension ana...

journal_title：Applied and environmental microbiology

authors： Labarre C,Diviès C,Guzzo J

更新日期：1996-12-01 00:00:00

abstract：:Vibrio cholerae identification based on molecular sequence data has been hampered by a lack of sequence variation from the closely related Vibrio mimicus. The two species share many genes coding for proteins, such as ctxAB, and show almost identical 16S DNA coding for rRNA (rDNA) sequences. Primers targeting conserved...

journal_title：Applied and environmental microbiology

authors： Chun J,Huq A,Colwell RR

更新日期：1999-05-01 00:00:00

abstract：:The genus Burkholderia includes over 60 species isolated from a wide range of environmental niches and can be tentatively divided into two major species clusters. The first cluster includes pathogens such as Burkholderia glumae, B. pseudomallei, and B. mallei and 17 well-studied species of the Burkholderia cepacia com...

journal_title：Applied and environmental microbiology

authors： Suárez-Moreno ZR,Devescovi G,Myers M,Hallack L,Mendonça-Previato L,Caballero-Mellado J,Venturi V

更新日期：2010-07-01 00:00:00

abstract：:The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to...

journal_title：Applied and environmental microbiology

authors： Gill AO,Holley RA

更新日期：2004-10-01 00:00:00

abstract：:Azospirillum is a rhizobacterial genus containing plant growth-promoting species associated with different crops worldwide. Azospirillum brasilense strains exhibit a growth-promoting effect by means of phytohormone production and possibly by N2 fixation. However, one of the most important factors for achieving an incr...

journal_title：Applied and environmental microbiology

authors： Stets MI,Alqueres SM,Souza EM,Pedrosa Fde O,Schmid M,Hartmann A,Cruz LM

更新日期：2015-10-01 00:00:00

abstract：:The levels of integron abundance and diversity in soil amended with pig slurry were studied. Real-time PCR illustrated a significant increase in class 1 integron prevalence after slurry application, with increased prevalence still evident at 10 months after application. Culture-dependent data revealed 10 genera, inclu...

journal_title：Applied and environmental microbiology

authors： Byrne-Bailey KG,Gaze WH,Zhang L,Kay P,Boxall A,Hawkey PM,Wellington EM

更新日期：2011-01-01 00:00:00

abstract：:Anaerobic methyl tert-butyl ether (MTBE) degradation potential was investigated in samples from a range of sources. From these 22 experimental variations, only one source (from wastewater treatment plant samples) exhibited MTBE degradation. These microcosms were methanogenic and were subjected to DNA-based stable isot...

journal_title：Applied and environmental microbiology

authors： Sun W,Sun X,Cupples AM

更新日期：2012-04-01 00:00:00

abstract：:Candida parapsilosis, C. tropicalis, and Torulopsis glabrata were the human-associated yeasts most frequently isolated from quahogs, oysters, and mussels collected from four estuarine areas along the northern shore of Long Island Sound. Some inconsistency and seasonal variation in the occurrence of these and other yea...

journal_title：Applied and environmental microbiology

authors： Buck JD,Bubucis PM,Combs TJ

更新日期：1977-02-01 00:00:00

abstract：:The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

journal_title：Applied and environmental microbiology

authors： Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

更新日期：2006-08-01 00:00:00

abstract：:Bacterioplankton community diversity was investigated in the subtropical Brisbane River-Moreton Bay estuary, Australia (27 degrees 25 minutes S, 153 degrees 5 minutes E). Bacterial communities were studied using automated rRNA intergenic spacer analysis (ARISA), which amplifies 16S-23S ribosomal DNA internally transcr...

journal_title：Applied and environmental microbiology

authors： Hewson I,Fuhrman JA

更新日期：2004-06-01 00:00:00

abstract：:The effect of addition of purified nisin Z in liposomes to cheese milk and of in situ production of nisin Z by Lactococcus lactis subsp. lactis biovar diacetylactis UL719 in the mixed starter on the inhibition of Listeria innocua in cheddar cheese was evaluated during 6 months of ripening. A cheese mixed starter cultu...

journal_title：Applied and environmental microbiology

authors： Benech RO,Kheadr EE,Laridi R,Lacroix C,Fliss I

更新日期：2002-08-01 00:00:00

abstract：:Five rumen bacteria, Selenomonas ruminantium, Bacteroides ruminicola, Megasphaera elsdenii, Butyrivibrio fibrisolvens, and Streptococcus bovis were grown in media containing nonlimiting concentrations of glucose, sucrose, maltose, cellobiose, xylose and/or lactate. Each bacterium was grown with every substrate that it...

journal_title：Applied and environmental microbiology

authors： Russell JB,Delfino FJ,Baldwin RL

更新日期：1979-03-01 00:00:00

abstract：:Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were...

journal_title：Applied and environmental microbiology

authors： Ahonen L,Tuovinen OH

更新日期：1990-02-01 00:00:00

abstract：:Renibacterium salmoninarum, a gram-positive diplococcobacillus, causes bacterial kidney disease, a condition that can result in extensive morbidity and mortality among stocks of fish. An immunodominant extracellular protein, called major soluble antigen (MSA), is encoded by two identical genes, msa1 and msa2. We found...

journal_title：Applied and environmental microbiology

authors： Rhodes LD,Coady AM,Deinhard RK

更新日期：2004-11-01 00:00:00

abstract：:Poultry are considered the major reservoir for Campylobacter jejuni, a leading bacterial cause of human food-borne diarrhea. To understand the ecology of C. jejuni and develop strategies to control C. jejuni infection in the animal reservoir, we initiated studies to examine the potential role of anti-Campylobacter mat...

journal_title：Applied and environmental microbiology

authors： Sahin O,Zhang Q,Meitzler JC,Harr BS,Morishita TY,Mohan R

更新日期：2001-09-01 00:00:00

abstract：:SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated. ...

journal_title：Applied and environmental microbiology

authors： Benbadis L,Garel JR,Hartley DL

更新日期：1991-12-01 00:00:00

abstract：:Two genetically engineered microorganisms (GEMs), Pseudomonas sp. strain B13 FR1(pFRC20P) (FR120) and Pseudomonas putida KT2440(pWWO-EB62) (EB62), were introduced into activated sludge microcosms that had the level of aeration, nutrient makeup, and microbial community structure of activated sludge reactors. FR120 cont...

journal_title：Applied and environmental microbiology

authors： Nüsslein K,Maris D,Timmis K,Dwyer DF

更新日期：1992-10-01 00:00:00