Prevalence, antigenic specificity, and bactericidal activity of poultry anti-Campylobacter maternal antibodies.

Abstract:

:Poultry are considered the major reservoir for Campylobacter jejuni, a leading bacterial cause of human food-borne diarrhea. To understand the ecology of C. jejuni and develop strategies to control C. jejuni infection in the animal reservoir, we initiated studies to examine the potential role of anti-Campylobacter maternal antibodies in protecting young broiler chickens from infection by C. jejuni. Using an enzyme-linked immunosorbent assay (ELISA), the prevalence of anti-C. jejuni antibodies in breeder chickens, egg yolks, and broilers from multiple flocks of different farms were examined. High levels of antibodies to the organism were detected in serum samples of breeder chickens and in egg yolk contents. To determine the dynamics of anti-Campylobacter maternal antibody transferred from yolks to hatchlings, serum samples collected from five broiler flocks at weekly intervals from 1 to 28 or 42 days of age were also examined by ELISA. Sera from the 1-day and 7-day-old chicks showed high titers of antibodies to C. jejuni. Thereafter, antibody titers decreased substantially and were not detected during the third and fourth weeks of age. The disappearance of anti-Campylobacter maternal antibodies during 3 to 4 weeks of age coincides with the appearance of C. jejuni infections observed in many broiler chicken flocks. As shown by immunoblotting, the maternally derived antibodies recognized multiple membrane proteins of C. jejuni ranging from 19 to 107 kDa. Moreover, in vitro serum bactericidal assays showed that anti-Campylobacter maternal antibodies were active in antibody-dependent complement-mediated killing of C. jejuni. Together, these results highlight the widespread presence of functional anti-Campylobacter antibodies in the poultry production system and provide a strong rationale for further investigation of the potential role of anti-C. jejuni maternal antibodies in protecting young chickens from infection by C. jejuni.

journal_name

Appl Environ Microbiol

authors

Sahin O,Zhang Q,Meitzler JC,Harr BS,Morishita TY,Mohan R

doi

10.1128/aem.67.9.3951-3957.2001

keywords:

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

3951-7

issue

9

eissn

0099-2240

issn

1098-5336

journal_volume

67

pub_type

杂志文章
  • Differential Expression of Virulence Genes and Motility in Ralstonia (Pseudomonas) solanacearum during Exponential Growth.

    abstract::A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed di...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.3.844-850.1997

    authors: Clough SJ,Flavier AB,Schell MA,Denny TP

    更新日期:1997-03-01 00:00:00

  • Life, death, and in-between: meanings and methods in microbiology.

    abstract::Determination of microbial viability by the plate count method is routine in microbiology laboratories worldwide. However, limitations of the technique, particularly with respect to environmental microorganisms, are widely recognized. Many alternatives based upon viability staining have been proposed, and these are of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/AEM.00744-11

    authors: Davey HM

    更新日期:2011-08-15 00:00:00

  • Ecological constraints limit the fitness of fungal hybrids in the Heterobasidion annosum species complex.

    abstract::The ability of two closely related species to maintain species boundaries in spite of retained interfertility between them is a documented driving force of speciation. Experimental evidence to support possible interspecific postzygotic isolation mechanisms for organisms belonging to the kingdom Fungi is still missing....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01135-07

    authors: Garbelotto M,Gonthier P,Nicolotti G

    更新日期:2007-10-01 00:00:00

  • Medium-chain fatty acids affect citrinin production in the filamentous fungus Monascus ruber.

    abstract::During submerged culture in the presence of glucose and glutamate, the filamentous fungus Monascus ruber produces water-soluble red pigments together with citrinin, a mycotoxin with nephrotoxic and hepatoxic effects on animals. Analysis of the (13)C-pigment molecules from mycelia cultivated with [1-(13)C]-, [2-(13)C]-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.3.1120-1125.2000

    authors: Hajjaj H,Klaébé A,Goma G,Blanc PJ,Barbier E,François J

    更新日期:2000-03-01 00:00:00

  • Critical evaluation of membrane supports for use in quantitative hybridizations.

    abstract::The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher & Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of dete...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.1.300-303.1996

    authors: Raskin L,Capman WC,Kane MD,Rittmann BE,Stahl DA

    更新日期:1996-01-01 00:00:00

  • Enhancement of the efficiency of secretion of heterologous lipase in Escherichia coli by directed evolution of the ABC transporter system.

    abstract::The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficienc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.7.3468-3474.2005

    authors: Eom GT,Song JK,Ahn JH,Seo YS,Rhee JS

    更新日期:2005-07-01 00:00:00

  • Heat inactivation of Mycobacterium avium-Mycobacterium intracellulare complex organisms in meat products.

    abstract::Wieners and sausages were prepared which contained the most heat-tolerant representative of the Mycobacterium avium-Mycobacterium intracellulare complex we were able to obtain. They also were prepared with infected tissues obtained from tuberculous swine. Processing conditions were as varied as possible. Neither incor...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.5.831-835.1979

    authors: Merkal RS,Crawford JA,Whipple DL

    更新日期:1979-11-01 00:00:00

  • Stereochemistry of furfural reduction by a Saccharomyces cerevisiae aldehyde reductase that contributes to in situ furfural detoxification.

    abstract::Ari1p from Saccharomyces cerevisiae, recently identified as an intermediate-subclass short-chain dehydrogenase/reductase, contributes in situ to the detoxification of furfural. Furfural inhibits efficient ethanol production by yeast, particularly when the carbon source is acid-treated lignocellulose, which contains fu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00542-10

    authors: Bowman MJ,Jordan DB,Vermillion KE,Braker JD,Moon J,Liu ZL

    更新日期:2010-08-01 00:00:00

  • Microbial diversity of septic tank effluent and a soil biomat.

    abstract::Microbial diversity of septic tank effluent (STE) and the biomat that is formed as a result of STE infiltration on soil were characterized by 16S rRNA gene sequence analysis. Results indicate that microbial communities are different within control soil, STE, and the biomat and that microbes found in STE are not found ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00560-08

    authors: Tomaras J,Sahl JW,Siegrist RL,Spear JR

    更新日期:2009-05-01 00:00:00

  • Chlorine injury and the enumeration of waterborne coliform bacteria.

    abstract::Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.3.633-641.1979

    authors: Camper AK,McFeters GA

    更新日期:1979-03-01 00:00:00

  • Use of root organ cultures to investigate the interaction between Glomus intraradices and Pratylenchus coffeae.

    abstract::The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was no...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.4308-4311.2003

    authors: Elsen A,Declerck S,De Waele D

    更新日期:2003-07-01 00:00:00

  • A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin.

    abstract::A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.3.1150-1152.1995

    authors: Loessner MJ,Schneider A,Scherer S

    更新日期:1995-03-01 00:00:00

  • Relationships of a novel Lyme disease spirochete, Borrelia spielmani sp. nov., with its hosts in Central Europe.

    abstract::To determine whether the pathogenic variant of Lyme disease spirochetes, isolate A14S, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various Central European sites for a related spirochete and determined its host association. A14S-like spirochetes infect numerous questing ti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.11.6414-6419.2004

    authors: Richter D,Schlee DB,Allgöwer R,Matuschka FR

    更新日期:2004-11-01 00:00:00

  • Prevalence of Clostridium botulinum in Finnish trout farms: pulsed-field gel electrophoresis typing reveals extensive genetic diversity among type E isolates.

    abstract::The distribution of Clostridium botulinum serotypes A, B, E, and F in Finnish trout farms was examined. A total of 333 samples were tested with a neurotoxin-specific PCR assay. C. botulinum type E was found in 68% of the farm sediment samples, in 15% of the fish intestinal samples, and in 5% of the fish skin samples. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.11.4161-4167.1998

    authors: Hielm S,Björkroth J,Hyytiä E,Korkeala H

    更新日期:1998-11-01 00:00:00

  • Percent G+C profiling accurately reveals diet-related differences in the gastrointestinal microbial community of broiler chickens.

    abstract::Broiler chickens from eight commercial farms in Southern Finland were analyzed for the structure of their gastrointestinal microbial community by a nonselective DNA-based method, percent G+C-based profiling. The bacteriological impact of the feed source and in-farm whole-wheat amendment of the diet was assessed by per...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.12.5656-5667.2001

    authors: Apajalahti JH,Kettunen A,Bedford MR,Holben WE

    更新日期:2001-12-01 00:00:00

  • Silage collected from dairy farms harbors an abundance of listeriaphages with considerable host range and genome size diversity.

    abstract::Since the food-borne pathogen Listeria monocytogenes is common in dairy farm environments, it is likely that phages infecting this bacterium ("listeriaphages") are abundant on dairy farms. To better understand the ecology and diversity of listeriaphages on dairy farms and to develop a diverse phage collection for furt...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01859-12

    authors: Vongkamjan K,Switt AM,den Bakker HC,Fortes ED,Wiedmann M

    更新日期:2012-12-01 00:00:00

  • Susceptibility of a field-derived, Bacillus thuringiensis-resistant strain of diamondback moth to in vitro-activated Cry1Ac toxin.

    abstract::Resistant and susceptible populations of the diamondback moth (Plutella xylostella) were tested with crystalline, solubilized, and partially and fully activated forms of the Bacillus thuringiensis Cry1Ac delta-endotoxin. Fully activated toxin greatly reduced the resistance ratio (ratio of the 50% lethal concentration ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.67.9.4372-4373.2001

    authors: Sayyed AH,Gatsi R,Kouskoura T,Wright DJ,Crickmore N

    更新日期:2001-09-01 00:00:00

  • Contribution of chitinases to Listeria monocytogenes pathogenesis.

    abstract::Listeria monocytogenes secretes two chitinases and one chitin binding protein. Mutants lacking chiA, chiB, or lmo2467 exhibited normal growth in cultured cells but were defective for growth in the livers and spleens of mice. Mammals lack chitin; thus, L. monocytogenes may have adapted chitinases to recognize alternati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01338-10

    authors: Chaudhuri S,Bruno JC,Alonzo F 3rd,Xayarath B,Cianciotto NP,Freitag NE

    更新日期:2010-11-01 00:00:00

  • Cyanuric Acid Biodegradation via Biuret: Physiology, Taxonomy, and Geospatial Distribution.

    abstract::Cyanuric acid is an industrial chemical produced during the biodegradation of s-triazine pesticides. The biodegradation of cyanuric acid has been elucidated using a single model system, Pseudomonas sp. strain ADP, in which cyanuric acid hydrolase (AtzD) opens the s-triazine ring and AtzEG deaminates the ring-opened pr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01964-19

    authors: Aukema KG,Tassoulas LJ,Robinson SL,Konopatski JF,Bygd MD,Wackett LP

    更新日期:2020-01-07 00:00:00

  • Sucker-like structures on the pathogenic amoeba Naegleria fowleri.

    abstract::Using scanning electron microscopy, we observed sucker-like structures on amoebae of 13 human isolates of Naegleria fowleri. The number of suckers per amoeba seemed to vary according to the virulence of the strain. We propose the term amoebastome to describe this unique sucker-like structure of N. fowleri. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.1.12-14.1984

    authors: John DT,Cole TB Jr,Marciano-Cabral FM

    更新日期:1984-01-01 00:00:00

  • Influence of hydrodynamics and cell signaling on the structure and behavior of Pseudomonas aeruginosa biofilms.

    abstract::Biofilms were grown from wild-type (WT) Pseudomonas aeruginosa PAO1 and the cell signaling lasI mutant PAO1-JP1 under laminar and turbulent flows to investigate the relative contributions of hydrodynamics and cell signaling for biofilm formation. Various biofilm morphological parameters were quantified using Image Str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.9.4457-4464.2002

    authors: Purevdorj B,Costerton JW,Stoodley P

    更新日期:2002-09-01 00:00:00

  • Metabolic engineering of a 1,2-propanediol pathway in Escherichia coli.

    abstract::1,2-Propanediol (1,2-PD) is a major commodity chemical that is currently derived from propylene, a nonrenewable resource. A goal of our research is to develop fermentation routes to 1,2-PD from renewable resources. Here we report the production of enantiomerically pure R-1,2-PD from glucose in Escherichia coli express...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.3.1180-1185.1999

    authors: Altaras NE,Cameron DC

    更新日期:1999-03-01 00:00:00

  • Production of a new D-amino acid oxidase from the fungus Fusarium oxysporum.

    abstract::The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.8.3750-3753.1999

    authors: Gabler M,Fischer L

    更新日期:1999-08-01 00:00:00

  • Identification of enterococci from broiler products and a broiler processing plant and description of Enterococcus viikkiensis sp. nov.

    abstract::In two previous studies dealing with lactic acid bacteria (LAB) from modified-atmosphere-packaged (MAP) broiler products and a broiler processing plant, several isolates remained unidentified. According to 16S rRNA gene sequence analysis, 36 isolates were assigned to the genus Enterococcus. Numerical analysis of combi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02412-10

    authors: Rahkila R,Johansson P,Säde E,Björkroth J

    更新日期:2011-02-01 00:00:00

  • Use of the Escherichia coli beta-Glucuronidase (gusA) Gene as a Reporter Gene for Analyzing Promoters in Lactic Acid Bacteria.

    abstract::[This corrects the article on p. 588 in vol. 60.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.6.2207-2207.1994

    authors:

    更新日期:1994-06-01 00:00:00

  • Characterization of a new pathway for epichlorohydrin degradation by whole cells of xanthobacter strain py2.

    abstract::The degradation of epichlorohydrin (3-chloropropylene oxide or 1-chloro-2,3-epoxypropane) by whole-cell suspensions of Xanthobacter strain Py2 was investigated. Cell suspensions prepared from cultures grown with propylene as the carbon source readily degraded epichlorohydrin. The ability to degrade epichlorohydrin cor...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.4.1507-1513.1995

    authors: Small FJ,Tilley JK,Ensign SA

    更新日期:1995-04-01 00:00:00

  • Hydrogen production by geobacter species and a mixed consortium in a microbial electrolysis cell.

    abstract::A hydrogen utilizing exoelectrogenic bacterium (Geobacter sulfurreducens) was compared to both a nonhydrogen oxidizer (Geobacter metallireducens) and a mixed consortium in order to compare the hydrogen production rates and hydrogen recoveries of pure and mixed cultures in microbial electrolysis cells (MECs). At an app...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01760-09

    authors: Call DF,Wagner RC,Logan BE

    更新日期:2009-12-01 00:00:00

  • Characterization of marine temperate phage-host systems isolated from Mamala Bay, Oahu, Hawaii.

    abstract::To understand the ecological and genetic role of viruses in the marine environment, it is critical to know the infectivity of viruses and the types of interactions that occur between marine viruses and their hosts. We isolated four marine phages from turbid plaques by using four indigenous bacterial hosts obtained fro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.2.535-542.1998

    authors: Jiang SC,Kellogg CA,Paul JH

    更新日期:1998-02-01 00:00:00

  • The weak acid preservative sorbic acid inhibits conidial germination and mycelial growth of Aspergillus niger through intracellular acidification.

    abstract::The growth of the filamentous fungus Aspergillus niger, a common food spoilage organism, is inhibited by the weak acid preservative sorbic acid (trans-trans-2,4-hexadienoic acid). Conidia inoculated at 10(5)/ml of medium showed a sorbic acid MIC of 4.5 mM at pH 4.0, whereas the MIC for the amount of mycelia at 24 h de...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.6.3506-3511.2004

    authors: Plumridge A,Hesse SJ,Watson AJ,Lowe KC,Stratford M,Archer DB

    更新日期:2004-06-01 00:00:00

  • Novel Trifunctional Xylanolytic Enzyme Axy43A from Paenibacillus curdlanolyticus Strain B-6 Exhibiting Endo-Xylanase, β-d-Xylosidase, and Arabinoxylan Arabinofuranohydrolase Activities.

    abstract::The axy43A gene encoding the intracellular trifunctional xylanolytic enzyme from Paenibacillus curdlanolyticus B-6 was cloned and expressed in Escherichia coli Recombinant PcAxy43A consisting of a glycoside hydrolase family 43 and a family 6 carbohydrate-binding module exhibited endo-xylanase, β-xylosidase, and arabin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02256-16

    authors: Teeravivattanakit T,Baramee S,Phitsuwan P,Waeonukul R,Pason P,Tachaapaikoon C,Sakka K,Ratanakhanokchai K

    更新日期:2016-12-01 00:00:00