Contribution of chitinases to Listeria monocytogenes pathogenesis.

abstract：:Vibrio vulnificus naturally inhabits a variety of aquatic organisms, including oysters, and is the leading cause of seafood-related death in the United States. Strains of this bacterium are genetically classified into environmental (E) and clinical (C) genotypes, which correlate with source of isolation. E-genotype st...

journal_title：Applied and environmental microbiology

authors： Williams TC,Ayrapetyan M,Oliver JD

更新日期：2014-03-01 00:00:00

abstract：:We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide so...

journal_title：Applied and environmental microbiology

authors： Liles MR,Williamson LL,Rodbumrer J,Torsvik V,Goodman RM,Handelsman J

更新日期：2008-05-01 00:00:00

abstract：:It has been suggested (L. H. Harris, R. P. Desai, N. E. Welker, and E. T. Papoutsakis, Biotechnol. Bioeng. 67:1-11, 2000) that butyryl phosphate (BuP) is a regulator of solventogenesis in Clostridium acetobutylicum. Here, we determined BuP and acetyl phosphate (AcP) levels in fermentations of C. acetobutylicum wild ty...

journal_title：Applied and environmental microbiology

authors： Zhao Y,Tomas CA,Rudolph FB,Papoutsakis ET,Bennett GN

更新日期：2005-01-01 00:00:00

abstract：:Listeria monocytogenes does not produce siderophores for iron acquisition. We demonstrate that a number of microbial siderophores and natural iron-binding compounds are able to promote the growth of iron-starved L. monocytogenes. We suggest that the ability of L. monocytogenes to use a variety of exogenous siderophore...

journal_title：Applied and environmental microbiology

authors： Simon N,Coulanges V,Andre P,Vidon DJ

更新日期：1995-04-01 00:00:00

abstract：:Suspension of adenovirus type 5 in 2.0 ml of cell culture fluid at 37 degrees C were subjected to smoke from four cigarettes over a 4-h period. The cigarettes were smoked in a normal manner, and the inhaled smoke was exhaled through glass tubing into the virus-containing fluid. The virus suspensions were then titrated...

journal_title：Applied and environmental microbiology

authors： Bardell D

更新日期：1978-11-01 00:00:00

abstract：:The ability of fractions of dissolved organic carbon (DOC) of different molecular weights (MW) to support bacterial growth was studied in batch culture experiments. Natural pelagic bacteria were inoculated into particle-free (0.2-mum filtered) water, taken from 10 oligotrophic lakes of differing humic content, and eit...

journal_title：Applied and environmental microbiology

authors： Tranvik LJ

更新日期：1990-06-01 00:00:00

abstract：:Pfiesteria piscicida is a heterotrophic dinoflagellate widely distributed along the middle Atlantic shore of the United States and associated with fish kills in the Neuse River (North Carolina) and the Chesapeake Bay (Maryland and Virginia). We constructed a genomic DNA library from clonally cultured P. piscicida and ...

journal_title：Applied and environmental microbiology

authors： Saito K,Drgon T,Robledo JA,Krupatkina DN,Vasta GR

更新日期：2002-11-01 00:00:00

abstract：:The form of a previously developed Bĕlehrádek type of growth rate model was used to develop a probability model for defining the growth/no growth interface as a function of temperature (10 to 37 degrees C), pH (pH 2.8 to 6.9), lactic acid concentration (0 to 500 mM), and water activity (0.955 to 0.999; NaCl was used a...

journal_title：Applied and environmental microbiology

authors： Presser KA,Ross T,Ratkowsky DA

更新日期：1998-05-01 00:00:00

abstract：:Nitrate was formed from ammonium at pH 3.2 to 6.1 in suspensions of a naturally acid forest soil; the maximum rates of formation occurred at ca. pH 4 to 5. Nitrate was also formed from soil nitrogen in suspensions incubated at 50 degrees C. Autotrophic nitrifying bacteria could not be isolated from this soil. Enrichme...

journal_title：Applied and environmental microbiology

authors： Stroo HF,Klein TM,Alexander M

更新日期：1986-11-01 00:00:00

abstract：:Shigella flexneri and Shigella sonnei bacteria cause the majority of all shigellosis cases worldwide. However, their distributions differ, with S. sonnei predominating in middle- and high-income countries and S. flexneri predominating in low-income countries. One proposed explanation for the continued range expansion ...

journal_title：Applied and environmental microbiology

authors： Watson J,Jenkins C,Clements A

更新日期：2018-04-16 00:00:00

abstract：:The utilization of gels, which are used for fluid drilling of seeds, as carriers of Bradyrhizobium japonicum for soybean (Glycine max (L.) Merr.) inoculation was studied. Gels of various chemical composition (magnesium silicate, potassium acrylate-acrylamide, grafted starch, and hydroxyethyl cellulose) were used, alth...

journal_title：Applied and environmental microbiology

authors： Jawson MD,Franzluebbers AJ,Berg RK

更新日期：1989-03-01 00:00:00

abstract：:Streptococcus mutans 6715-15 and Streptococcus sanguis 10558 were grown together in continuous culture with glucose as the limiting carbon source. The relationship of growth rate to substrate concentration was determined for pure cultures of each organism in continuous and batch cultures. A model based on competition ...

journal_title：Applied and environmental microbiology

authors： Kemp CW,Robrish SA,Curtis MA,Sharer SA,Bowen WH

更新日期：1983-04-01 00:00:00

abstract：:Male-specific RNA coliphages (F-RNA coliphages) have been proposed as a potential viral indicator of fecal contamination in water and foods because they are easy to culture and are a normal component of the mammalian gut flora. F-RNA coliphage plaque numbers are typically obtained by directly plating a 10-fold dilutio...

journal_title：Applied and environmental microbiology

authors： Jones TH,Johns MW

更新日期：2009-10-01 00:00:00

abstract：:A simple and rapid radioimmunoassay was developed for the quantitative determination of ochratoxin A in barley. [14C]ochratoxin A, with a specific activity of 130 Ci/mol, was used as the tracer. Toxin levels below 100 ng/ml required a cleanup step. Three methods (the Association of Official Analytical Chemists cleanup...

journal_title：Applied and environmental microbiology

authors： Rousseau DM,Slegers GA,Van Peteghem CH

更新日期：1985-08-01 00:00:00

abstract：:Cells of Bradyrhizobium japonicum were grown in media containing either 1.0 mM or 0.5 muM phosphorus. In growth pouch experiments, infection of the primary root of soybean (Glycine max (L.) Merr.) by B. japonicum USDA 31, 110, and 142 was significantly delayed when P-limited cells were applied to the root. In a greenh...

journal_title：Applied and environmental microbiology

authors： Mullen MD,Israel DW,Wollum AG

更新日期：1988-10-01 00:00:00

abstract：:Spontaneous vegetable fermentations, with their rich flavors and postulated health benefits, are regaining popularity. However, their microbiology is still poorly understood, therefore raising concerns about food safety. In addition, such spontaneous fermentations form interesting cases of man-made microbial ecosystem...

journal_title：Applied and environmental microbiology

authors： Wuyts S,Van Beeck W,Oerlemans EFM,Wittouck S,Claes IJJ,De Boeck I,Weckx S,Lievens B,De Vuyst L,Lebeer S

更新日期：2018-05-31 00:00:00

abstract：:Seabird ticks are known reservoirs of bacterial pathogens of medical importance; however, ticks parasitizing tropical seabirds have received less attention than their counterparts from temperate and subpolar regions. Recently, Rickettsia africae was described to infect seabird ticks of the western Indian Ocean and New...

journal_title：Applied and environmental microbiology

authors： Wilkinson DA,Dietrich M,Lebarbenchon C,Jaeger A,Le Rouzic C,Bastien M,Lagadec E,McCoy KD,Pascalis H,Le Corre M,Dellagi K,Tortosa P

更新日期：2014-06-01 00:00:00

abstract：:The biodegradation of polycyclic aromatic hydrocarbon pollutants is constrained, in part, by their solid physical state and very low water solubility. Searching for ways to overcome these limitations, we isolated from soil a bacterium capable of growing on pyrene as a sole source of carbon and energy. Acid-fast stain,...

journal_title：Applied and environmental microbiology

authors： Jimenez IY,Bartha R

更新日期：1996-07-01 00:00:00

abstract：:Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 microns in diameter and theoretically could contain several hundred bacteria. Viable L. pneumophila cells were observed within vesicles which had been exposed to two co...

journal_title：Applied and environmental microbiology

authors： Berk SG,Ting RS,Turner GW,Ashburn RJ

更新日期：1998-01-01 00:00:00

abstract：:The need for protozoa for the proliferation of Legionella pneumophila in aquatic habitats is still not fully understood and is even questioned by some investigators. This study shows the in vivo growth of L. pneumophila in protozoa in aquatic biofilms developing at high concentrations on plasticized polyvinyl chloride...

journal_title：Applied and environmental microbiology

authors： Kuiper MW,Wullings BA,Akkermans AD,Beumer RR,van der Kooij D

更新日期：2004-11-01 00:00:00

abstract：:Five strains of Aspergillus fumigatus (A, B, D, H, and K) isolated from sawmills were used to expose groups of three rats by intratracheal intubation. The dose was 10(9) spores per rat. At 48 h after administration, two rats from the D group and all rats from the K group died with symptoms of strong dyspnea and tachyp...

journal_title：Applied and environmental microbiology

authors： Land CJ,Sostarić B,Fuchs R,Lundström H,Hult K

更新日期：1989-11-01 00:00:00

abstract：:The effects of sublethal concentrations of a variety of chemicals on the susceptibility of rainbow trout (Oncorhynchus mykiss) juveniles to Saprolegnia parasitica infection was examined. Sublethal concentrations of un-ionized ammonia (0.05 mg/liter) and nitrite (0.12 mg/liter) increased fish susceptibility after 10 da...

journal_title：Applied and environmental microbiology

authors： Carballo M,Muñoz MJ

更新日期：1991-06-01 00:00:00

abstract：:Marine infaunal burrows and tubes greatly enhance solute transport between sediments and the overlying water column and are sites of elevated microbial activity. Biotic and abiotic controls of the compositions and activities of burrow and tube microbial communities are poorly understood. The microbial communities in t...

journal_title：Applied and environmental microbiology

authors： Matsui GY,Ringelberg DB,Lovell CR

更新日期：2004-12-01 00:00:00

abstract：:To identify genes that are important for class IIa bacteriocin interaction and resistance in Listeria species, transposon Tn917 knockout libraries were constructed for Listeria innocua strain Lin11 and screened for mutants that are resistant to pediocin AcH. A highly resistant mutant (G7) (MIC > 20 microg/ml; 1,000-fo...

journal_title：Applied and environmental microbiology

authors： Xue J,Hunter I,Steinmetz T,Peters A,Ray B,Miller KW

更新日期：2005-03-01 00:00:00

abstract：:A simple gel immunodiffusion agar procedure was developed for detecting toxigenic strains of Clostridium botulinum type A. The method consisted of overlaying colonies grown on thin-layer tryptone-peptone-glucose-yeast extract agar with gel diffusion agar containing desired levels of C. botulinum type A antitoxin. Conc...

journal_title：Applied and environmental microbiology

authors： Ferreira JL,Hamdy MK,Zapatka FA,Hebert WO

更新日期：1981-12-01 00:00:00

abstract：:Reporter gene technology was used to observe the regulation of the alginate biosynthesis gene, algC in a mucoid strain of Pseudomonas aeruginosa in developing and mature biofilms in continuous culture on Teflon and glass substrata. The plasmid pNZ63, carrying an algC-lacZ transcriptional fusion, was shown to not be di...

journal_title：Applied and environmental microbiology

authors： Davies DG,Geesey GG

更新日期：1995-03-01 00:00:00

abstract：:This study was aimed at assessing whether the repeated exposure of 12 strains of Salmonella spp., Escherichia coli, and Listeria monocytogenes to alternative nonthermal decontamination techniques with UV light (UV-C) and nonthermal atmospheric plasma (NTAP) may cause the emergence of variants showing increased resista...

journal_title：Applied and environmental microbiology

authors： Álvarez-Molina A,de Toro M,Ruiz L,López M,Prieto M,Alvarez-Ordóñez A

更新日期：2020-04-17 00:00:00

abstract：:The culturability of three Campylobacter jejuni strains and their infectivity for day-old chicks were assessed following storage of the strains in saline. The potential for colonization of chicks was weakened during the storage period and terminated 3 to 4 weeks before the strains became nonculturable. The results fro...

journal_title：Applied and environmental microbiology

authors： Hald B,Knudsen K,Lind P,Madsen M

更新日期：2001-05-01 00:00:00

abstract：:Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was ligated to the commercial vector pCR2.1-TOPO, and selectable markers were added so that T. kodakaraensis transformants could...

journal_title：Applied and environmental microbiology

authors： Santangelo TJ,Cubonová L,Reeve JN

更新日期：2008-05-01 00:00:00

abstract：:The ability of microorganisms to perform dissimilatory (per)chlorate reduction is, for most species, known to be oxygen sensitive. Consequently, bioremediation processes for the removal of oxochlorates will be disturbed if oxygen is present. We measured the expression of chlorite dismutase and chlorate reductase in th...

journal_title：Applied and environmental microbiology

authors： Lindqvist MH,Johansson N,Nilsson T,Rova M

更新日期：2012-06-01 00:00:00