Abstract:
:Biofilms were grown from wild-type (WT) Pseudomonas aeruginosa PAO1 and the cell signaling lasI mutant PAO1-JP1 under laminar and turbulent flows to investigate the relative contributions of hydrodynamics and cell signaling for biofilm formation. Various biofilm morphological parameters were quantified using Image Structure Analyzer software. Multivariate analysis demonstrated that both cell signaling and hydrodynamics significantly (P < 0.000) influenced biofilm structure. In turbulent flow, both biofilms formed streamlined patches, which in some cases developed ripple-like wave structures which flowed downstream along the surface of the flow cell. In laminar flow, both biofilms formed monolayers interspersed with small circular microcolonies. Ripple-like structures also formed in four out of six WT biofilms, although their velocity was approximately 10 times less than that of those that formed in the turbulent flow cells. The movement of biofilm cell clusters over solid surfaces may have important clinical implications for the dissemination of biofilm subject to fluid shear, such as that found in catheters. The ability of the cell signaling mutant to form biofilms in high shear flow demonstrates that signaling mechanisms are not required for the formation of strongly adhered biofilms. Similarity between biofilm morphologies in WT and mutant biofilms suggests that the dilution of signal molecules by mass transfer effects in faster flowing systems mollifies the dramatic influence of signal molecules on biofilm structure reported in previous studies.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Purevdorj B,Costerton JW,Stoodley Pdoi
10.1128/aem.68.9.4457-4464.2002keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
4457-64issue
9eissn
0099-2240issn
1098-5336journal_volume
68pub_type
杂志文章abstract::In the present work, we aimed at determining the relationship between the hepatitis A virus (HAV) numbers in imported frozen coquina clams involved in two hepatitis outbreaks, as well as the risk for human health. Due to HAV unculturability, a standardized TaqMan real-time reverse transcription-PCR controlling the vir...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01177-09
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pub_type: 杂志文章
doi:10.1128/aem.70.1.96-103.2004
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.4.1221-1226.1994
更新日期:1994-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.11.6698-6702.2003
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.4.971-976.1990
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.2.294-298.1984
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.6.2011-2015.1992
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.5.1056-1062.1986
更新日期:1986-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.6.1505-1508.1985
更新日期:1985-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.38.4.734-739.1979
更新日期:1979-10-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06434-11
更新日期:2011-12-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
doi:10.1128/aem.66.2.571-577.2000
更新日期:2000-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.63.2.474-481.1997
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.9.3454-3456.1995
更新日期:1995-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.12.5303-5306.1999
更新日期:1999-12-01 00:00:00
abstract::Escherichia coli KO11 was previously constructed to produce ethanol from acid hydrolysates of hemicellulose (pentoses and hexoses) by the chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB). Klebsiella oxytoca P2 was constructed in an analogous fash...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.12.4633-4637.1997
更新日期:1997-12-01 00:00:00
abstract::Thirty-three enteric isolates from Australian (Escherichia coli only) and United Kingdom (U.K.) (Salmonella sp., Citrobacter spp., and E. coli) piggeries were characterized with respect to their copper resistance. The copper resistance phenotypes of four new Australian E. coli isolates were comparable with that of the...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.8.2531-2537.1993
更新日期:1993-08-01 00:00:00
abstract::The utilization of gels, which are used for fluid drilling of seeds, as carriers of Bradyrhizobium japonicum for soybean (Glycine max (L.) Merr.) inoculation was studied. Gels of various chemical composition (magnesium silicate, potassium acrylate-acrylamide, grafted starch, and hydroxyethyl cellulose) were used, alth...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.3.617-622.1989
更新日期:1989-03-01 00:00:00
abstract::One emerging disease of grapevine in Europe is Bois noir (BN), a phytoplasmosis caused by "Candidatus Phytoplasma solani" and spread in vineyards by the planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae). Here we present the first full characterization of the bacterial community of this important disease vector co...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02121-10
更新日期:2011-02-01 00:00:00
abstract::A number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. No single one of the indicators tested to date has been able to determine the source of fecal pollution in water. However, the combined use of different indicators has been demonstrat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02350-09
更新日期:2010-03-01 00:00:00