Influences of biofilm structure and antibiotic resistance mechanisms on indirect pathogenicity in a model polymicrobial biofilm.

abstract：:Monoclonal antibodies (MAbs) against Vibrio species that infect humans, fish, and shellfish were developed for application in rapid identifications. The pathogens included Vibrio alginolyticus, V. anguillarum, V. carchariae, V. cholerae, V. damsela, V. furnissii, V. harveyi, V. ordalii, V. parahaemolyticus, and V. vul...

journal_title：Applied and environmental microbiology

authors： Chen D,Hanna PJ,Altmann K,Smith A,Moon P,Hammond LS

更新日期：1992-11-01 00:00:00

abstract：:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

journal_title：Applied and environmental microbiology

authors： Lauber CL,Hamady M,Knight R,Fierer N

更新日期：2009-08-01 00:00:00

abstract：:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium which can grow at NaCl concentrations up to 3.0 M and at pH values up to 11. The genome sequence revealed that the cyanobacterium Synechocystis sp. strain PCC 6803 contains five putative Na+/H+ antiporters, two of which are homologous to NhaP of Pse...

journal_title：Applied and environmental microbiology

authors： Wutipraditkul N,Waditee R,Incharoensakdi A,Hibino T,Tanaka Y,Nakamura T,Shikata M,Takabe T,Takabe T

更新日期：2005-08-01 00:00:00

abstract：:Enterococcus faecalis encodes a biotin-dependent oxaloacetate decarboxylase (OAD), which is constituted by four subunits: E. faecalis carboxyltransferase subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and the novel subunit Ef-H. Our results show that in E. faecalis, subunits Ef-A, Ef-D, ...

journal_title：Applied and environmental microbiology

authors： Repizo GD,Blancato VS,Mortera P,Lolkema JS,Magni C

更新日期：2013-05-01 00:00:00

abstract：:The extremely thermophilic anaerobic archaeon strain B1001 was isolated from a hot-spring environment in Japan. The cells were irregular cocci, 0.5 to 1.0 micrometers in diameter. The new isolate grew at temperatures between 60 and 95 degrees C (optimum, 85 degrees C), from pH 5.0 to 9.0 (optimum, pH 7.0), and from 1....

journal_title：Applied and environmental microbiology

authors： Tachibana Y,Kuramura A,Shirasaka N,Suzuki Y,Yamamoto T,Fujiwara S,Takagi M,Imanaka T

更新日期：1999-05-01 00:00:00

abstract：:In this article, a new mechanism influencing the transport of microorganisms through unsaturated porous media is examined, and a new method for directly visualizing bacterial behavior within a porous medium under controlled chemical and flow conditions is introduced. Resting cells of hydrophilic and relatively hydroph...

journal_title：Applied and environmental microbiology

authors： Wan J,Wilson JL,Kieft TL

更新日期：1994-02-01 00:00:00

abstract：:Genome-wide analysis has revealed abundant FabG (beta-ketoacyl-ACP reductase) paralogs, with uncharacterized biological functions, in several halophilic archaea. In this study, we identified for the first time that the fabG1 gene, but not the other five fabG paralogs, encodes the polyhydroxyalkanoate (PHA)-specific ac...

journal_title：Applied and environmental microbiology

authors： Han J,Lu Q,Zhou L,Liu H,Xiang H

更新日期：2009-10-01 00:00:00

abstract：:Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. cris...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Lipman LJ,van Knapen F,Biesterveld S

更新日期：2002-02-01 00:00:00

abstract：:The use of bioluminescence as a sensitive marker for detection of Pseudomonas spp. in the rhizosphere was investigated. Continuous expression of the luxCDABE genes, required for bioluminescence, was not detectable in the rhizosphere. However, when either a naphthalene-inducible luxCDABE construct or a constitutive lux...

journal_title：Applied and environmental microbiology

authors： de Weger LA,Dunbar P,Mahafee WF,Lugtenberg BJ,Sayler GS

更新日期：1991-12-01 00:00:00

abstract：:A latent virus-like agent, which we designated zooxanthella filamentous virus 1 (ZFV1), was isolated from Symbiodinium sp. strain CCMP 2465 and characterized. Transmission electron microscopy and analytical flow cytometry revealed the presence of a new group of distinctive filamentous virus-like particles after exposu...

journal_title：Applied and environmental microbiology

authors： Lohr J,Munn CB,Wilson WH

更新日期：2007-05-01 00:00:00

abstract：:Cold shock at 0 to 15 degrees C for 1 to 3 h increased the thermal sensitivity of Listeria monocytogenes. In a model broth system, thermal death time at 60 degrees C was reduced by up to 45% after L. monocytogenes Scott A was cold shocked for 3 h. The duration of the cold shock affected thermal tolerance more than did...

journal_title：Applied and environmental microbiology

authors： Miller AJ,Bayles DO,Eblen BS

更新日期：2000-10-01 00:00:00

abstract：:Exopolysaccharide-producing Lactobacillus casei CG11 was isolated from soft, white, homemade cheese. In basal minimal medium, it produces a neutral heteropolysaccharide consisting predominantly of glucose (about 75%) and rhamnose (about 15%). Plasmid curing experiments revealed that exopolysaccharide production by str...

journal_title：Applied and environmental microbiology

authors： Kojic M,Vujcic M,Banina A,Cocconcelli P,Cerning J,Topisirovic L

更新日期：1992-12-01 00:00:00

abstract：:A bacterium, strain BIS-6, that grew aerobically on dimethylsulfoniopropionate (DMSP) was isolated from an intertidal mud sample. Strain BIS-6 quantitatively demethylated DMSP and 3-methiolpropionate to 3-mercaptopropionate. Strain BIS-6 was a versatile methylotroph growing on the osmolytes DMSP and glycine betaine an...

journal_title：Applied and environmental microbiology

authors： Visscher PT,Taylor BF

更新日期：1994-12-01 00:00:00

abstract：:A plasmid marker rescue system based on restoration of the nptII gene was established in Streptococcus gordonii to study the transfer of bacterial and transgenic plant DNA by transformation. In vitro studies revealed that the marker rescue efficiency depends on the type of donor DNA. Plasmid and chromosomal DNA of bac...

journal_title：Applied and environmental microbiology

authors： Kharazmi M,Sczesny S,Blaut M,Hammes WP,Hertel C

更新日期：2003-10-01 00:00:00

abstract：:Oxolinic acid (OA), a quinolone, inhibits the activity of DNA gyrase composed of GyrA and GyrB and shows antibacterial activity against Burkholderia glumae. Since B. glumae causes bacterial seedling rot and grain rot of rice, both of which are devastating diseases, the emergence of OA-resistant bacteria has important ...

journal_title：Applied and environmental microbiology

authors： Maeda Y,Kiba A,Ohnishi K,Hikichi Y

更新日期：2004-09-01 00:00:00

abstract：:The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of cdh-1 and cdh-2 exhibit 97% similarity. A total of eighty-six point mutations between cdh-1 and cdh-2 are observed. Both...

journal_title：Applied and environmental microbiology

authors： Li B,Nagalla SR,Renganathan V

更新日期：1997-02-01 00:00:00

abstract：:The prokaryote community activity and structural characteristics within marine sediment sampled across a continental shelf area located off eastern Antarctica (66 degrees S, 143 degrees E; depth range, 709 to 964 m) were studied. Correlations were found between microbial biomass and aminopeptidase and chitinase rates,...

journal_title：Applied and environmental microbiology

authors： Bowman JP,McCammon SA,Gibson JA,Robertson L,Nichols PD

更新日期：2003-05-01 00:00:00

abstract：:The diversity of the predominant bacteria in the human gastrointestinal tract was studied by using 16S rRNA-based approaches. PCR amplicons of the V6 to V8 regions of fecal 16S rRNA and ribosomal DNA (rDNA) were analyzed by temperature gradient gel electrophoresis (TGGE). TGGE of fecal 16S rDNA amplicons from 16 indiv...

journal_title：Applied and environmental microbiology

authors： Zoetendal EG,Akkermans AD,De Vos WM

更新日期：1998-10-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris has been utilized for heterologous protein expression for over 30 years. Because P. pastoris secretes few of its own proteins, the exported recombinant protein is the major polypeptide in the extracellular medium, making purification relatively easy. Unfortunately, some recombi...

journal_title：Applied and environmental microbiology

authors： Naranjo CA,Jivan AD,Vo MN,de Sa Campos KH,Deyarmin JS,Hekman RM,Uribe C,Hang A,Her K,Fong MM,Choi JJ,Chou C,Rabara TR,Myers G,Moua P,Thor,Risser DD,Vierra CA,Franz AH,Lin-Cereghino J,Lin-Cereghino GP

更新日期：2019-11-27 00:00:00

abstract：:The Alvord Basin in southeast Oregon contains a variety of hydrothermal features which have never been microbiologically characterized. A sampling of Murky Pot (61 degrees C; pH 7.1) led to the isolation of a novel arsenic-metabolizing organism (YeAs) which produces an arsenic sulfide mineral known as beta-realgar, a ...

journal_title：Applied and environmental microbiology

authors： Ledbetter RN,Connon SA,Neal AL,Dohnalkova A,Magnuson TS

更新日期：2007-09-01 00:00:00

abstract：:The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was no...

journal_title：Applied and environmental microbiology

authors： Elsen A,Declerck S,De Waele D

更新日期：2003-07-01 00:00:00

abstract：:The genes encoding NAD(+)-dependent alanine dehydrogenases (AlaDHs) (EC 1.4.1.1) from the Antarctic bacterial organisms Shewanella sp. strain Ac10 (SheAlaDH) and Carnobacterium sp. strain St2 (CarAlaDH) were cloned and expressed in Escherichia coli. Of all of the AlaDHs that have been sequenced, SheAlaDH exhibited the...

journal_title：Applied and environmental microbiology

authors： Galkin A,Kulakova L,Ashida H,Sawa Y,Esaki N

更新日期：1999-09-01 00:00:00

abstract：:"Caldocellum saccharolyticum" is an obligatory anaerobic thermophilic bacterium. A gene from this organism, designated celB, has been cloned in Escherichia coli as part of a bacteriophage lambda gene library. This gene produces a thermostable cellulase that shows both endoglucanase and exoglucanase activities on test ...

journal_title：Applied and environmental microbiology

authors： Saul DJ,Williams LC,Grayling RA,Chamley LW,Love DR,Bergquist PL

更新日期：1990-10-01 00:00:00

abstract：:Electrophoretic karyotypes (EKs) of 83 isolates were variable within agricultural and natural populations of Sclerotinia sclerotiorum, as well as among S. sclerotiorum, Sclerotinia minor, and Sclerotinia trifoliorum. Variation in EKs was not observed within six mitotic or three meiotic lineages of isolates. EKs of 8 t...

journal_title：Applied and environmental microbiology

authors： Errampalli D,Kohn LM

更新日期：1996-11-01 00:00:00

abstract：:The interaction of the peptide antibiotic nisin with liposomes has been studied. The effect of this interaction was analyzed on the membrane potential (inside negative) and the pH gradient (inside alkaline) in liposomes made from Escherichia coli phosphatidylethanolamine and egg phosphatidylcholine (9:1, wt/wt). The m...

journal_title：Applied and environmental microbiology

authors： Gao FH,Abee T,Konings WN

更新日期：1991-08-01 00:00:00

abstract：:The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

journal_title：Applied and environmental microbiology

authors： Schendel FJ,Flickinger MC

更新日期：1992-09-01 00:00:00

abstract：:Botulinum neurotoxin (BoNT) serotype B (BoNT/B) is one of the serotypes of BoNT that causes deadly human botulism, though it is used clinically for treatment of many neuromuscular diseases. BoNT/B is produced by Clostridium botulinum, and it is secreted along with a group of neurotoxin-associated proteins (NAPs) in th...

journal_title：Applied and environmental microbiology

authors： Wang HH,Riding S,Lindo P,Singh BR

更新日期：2010-10-01 00:00:00

abstract：:A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed di...

journal_title：Applied and environmental microbiology

authors： Clough SJ,Flavier AB,Schell MA,Denny TP

更新日期：1997-03-01 00:00:00

abstract：:Values of Delta(34)S (=delta(34)S(HS)-delta(34)S(SO(4)), where delta(34)S(HS) and delta(34)S(SO(4)) indicate the differences in the isotopic compositions of the HS(-) and SO(4)(2-) in the eluent, respectively) for many modern marine sediments are in the range of -55 to -75 per thousand, much greater than the -2 to -46...

journal_title：Applied and environmental microbiology

authors： Davidson MM,Bisher ME,Pratt LM,Fong J,Southam G,Pfiffner SM,Reches Z,Onstott TC

更新日期：2009-09-01 00:00:00

abstract：:Phytoplankton replace phosphorus-containing lipids (P-lipids) with non-P analogues, boosting growth in P-limited oceans. In the model diatom Thalassiosira pseudonana, the substitution dynamics of lipid headgroups are well described, but those of the individual lipids, differing in fatty acid composition, are unknown. ...

journal_title：Applied and environmental microbiology

authors： Hunter JE,Brandsma J,Dymond MK,Koster G,Moore CM,Postle AD,Mills RA,Attard GS

更新日期：2018-03-01 00:00:00