Electrophoretic Karyotypes of Sclerotinia sclerotiorum.

abstract：:The plasmid profiles of 619 cultures of Bacillus anthracis which had been isolated and stored between 1954 and 1989 were analyzed using the Laboratory Response Network real-time PCR assay targeting a chromosomal marker and both virulence plasmids (pXO1 and pXO2). The cultures were stored at ambient temperature on tryp...

journal_title：Applied and environmental microbiology

authors： Marston CK,Hoffmaster AR,Wilson KE,Bragg SL,Plikaytis B,Brachman P,Johnson S,Kaufmann AF,Popovic T

更新日期：2005-12-01 00:00:00

abstract：:Fluorescent antibodies (FAs) prepared against 16 ammonia-oxidizing nitrifying bacteria were examined as to cross-reactivity in heterologous FA staining tests. Virtually all cross-reactions to the seven Nitrosomonas FAs were confined to the Nitrosomonas isolates. The five Nitrosospira, three Nitrosolobus, and one Nitro...

journal_title：Applied and environmental microbiology

authors： Belser LW,Schmidt EL

更新日期：1978-10-01 00:00:00

abstract：:We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, s...

journal_title：Applied and environmental microbiology

authors： Rollins DM,Coolbaugh JC,Walker RI,Weiss E

更新日期：1983-01-01 00:00:00

abstract：:Chitin is an abundant biopolymer whose degradation is mediated primarily by bacterial chitinases. We developed a degenerate PCR primer set to amplify a approximately 900-bp fragment of family 18, group I chitinase genes and used it to retrieve these gene fragments from environmental samples. Clone libraries of presump...

journal_title：Applied and environmental microbiology

authors： LeCleir GR,Buchan A,Hollibaugh JT

更新日期：2004-12-01 00:00:00

abstract：:Several investigations have identified benzothiophene-2,3-dione in the organic solvent extracts of acidified cultures degrading dibenzothiophene via the Kodama pathway. In solution at neutral pH, the 2,3-dione exists as 2-mercaptophenylglyoxylate, which cyclizes upon acidification and is extracted as the 2,3-dione. Th...

journal_title：Applied and environmental microbiology

authors： Bressler DC,Fedorak PM

更新日期：2001-11-01 00:00:00

abstract：:The esterases and lipases from the α/β hydrolase superfamily exhibit an enormous sequence diversity, fold plasticity, and activities. Here, we present the comprehensive sequence and biochemical analyses of seven distinct esterases and lipases from the metagenome of Lake Arreo, an evaporite karstic lake in Spain (42°46...

journal_title：Applied and environmental microbiology

authors： Martínez-Martínez M,Alcaide M,Tchigvintsev A,Reva O,Polaina J,Bargiela R,Guazzaroni ME,Chicote A,Canet A,Valero F,Rico Eguizabal E,Guerrero Mdel C,Yakunin AF,Ferrer M

更新日期：2013-06-01 00:00:00

abstract：:In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. strain LB126 were investigated. The alpha and beta subunits of a dioxygenase complex (FlnA1-FlnA2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran d...

journal_title：Applied and environmental microbiology

authors： Schuler L,Ní Chadhain SM,Jouanneau Y,Meyer C,Zylstra GJ,Hols P,Agathos SN

更新日期：2008-02-01 00:00:00

abstract：:In this study, a number of Listeria monocytogenes strains of different origins were evaluated for in vitro invasion capacity for various human cell types (monocytic THP-1, enterocytic Caco-2, and hepatocytic HepG2 cells) and for expression levels of specific virulence genes. For THP-1 cells, no differences between cli...

journal_title：Applied and environmental microbiology

authors： Werbrouck H,Grijspeerdt K,Botteldoorn N,Van Pamel E,Rijpens N,Van Damme J,Uyttendaele M,Herman L,Van Coillie E

更新日期：2006-06-01 00:00:00

abstract：:Leptospirosis, an emerging zoonotic disease, remains poorly understood because of a lack of genetic manipulation tools available for pathogenic leptospires. Current genetic manipulation techniques include insertion of DNA by random transposon mutagenesis and homologous recombination via suicide vectors. This study des...

journal_title：Applied and environmental microbiology

authors： Pappas CJ,Benaroudj N,Picardeau M

更新日期：2015-05-01 00:00:00

abstract：:PCR generated two distinct products from a toxic isolate of Alexandrium catenella, which had been taken from Dai Ya Bay (southern China), by using primers for large-subunit rRNA. This pattern is distinct from published data for North American Alexandrium species. Sequences of the two products suggest that the smaller ...

journal_title：Applied and environmental microbiology

authors： Yeung PK,Kong KF,Wong FT,Wong JT

更新日期：1996-11-01 00:00:00

abstract：:Polymorphism of the nuclear ribosomal DNA intergenic spacer (IGS) of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum was studied to evaluate whether this sequence could be used in field studies to estimate the diversity of strains forming mycorrhizas on individual Pinus pinaster root systems. This sequence w...

journal_title：Applied and environmental microbiology

authors： Guidot A,Lumini E,Debaud JC,Marmeisse R

更新日期：1999-03-01 00:00:00

abstract：:Thiobacillus ferrooxidans AP19-3 oxidized molybdenum blue (Mo) enzymatically. Molybdenum oxidase in the plasma membrane of this bacterium was purified ca. 77-fold compared with molybdenum oxidase in cell extract. A purified molybdenum oxidase showed characteristic absorption maxima due to reduced-type cytochrome oxida...

journal_title：Applied and environmental microbiology

authors： Sugio T,Hirayama K,Inagaki K,Tanaka H,Tano T

更新日期：1992-05-01 00:00:00

abstract：:The biodegradation of tert-butylphenyl diphenyl phosphate (BPDP) was examined in microcosms containing sediment and water from five different ecosystems as part of our studies to elucidate the environmental fate of phosphate ester flame retardants. Biodegradation of [14C]BPDP was monitored in the environmental microco...

journal_title：Applied and environmental microbiology

authors： Heitkamp MA,Freeman JP,Cerniglia CE

更新日期：1986-02-01 00:00:00

abstract：:Streptococcus iniae is a major pathogen of fish, producing fatal disease among fish species living in very diverse environments. Recently, reoccurrences of disease outbreaks were recorded in rainbow trout (Oncorhynchus mykiss, Walbaum) farms where the entire fish population was routinely vaccinated. New strains are di...

journal_title：Applied and environmental microbiology

authors： Eyngor M,Tekoah Y,Shapira R,Hurvitz A,Zlotkin A,Lublin A,Eldar A

更新日期：2008-11-01 00:00:00

abstract：:Significant knowledge gaps exist in our understanding of Campylobacter jejuni contamination of the poultry production continuum. Microbiological surveillance and genotypic characterization were undertaken on C. jejuni isolates longitudinally recovered from three poultry farms (weekly samples), the abattoir at which bi...

journal_title：Applied and environmental microbiology

authors： Inglis GD,Ramezani N,Taboada EN,Boras VF,Uwiera RRE

更新日期：2021-01-15 00:00:00

abstract：:A major barrier to the use of nitrogen-fixing inoculum strains for the enhancement of legume productivity is the inability of commercially available strains to compete with indigenous rhizobia for nodule formation. Despite extensive research on nodulation competitiveness, there are no examples of field efficacy studie...

journal_title：Applied and environmental microbiology

authors： Robleto EA,Kmiecik K,Oplinger ES,Nienhuis J,Triplett EW

更新日期：1998-07-01 00:00:00

abstract：:Escherichia coli K-12 W3110 grows in the presence of membrane-permeant organic acids that can depress cytoplasmic pH and accumulate in the cytoplasm. We conducted experimental evolution by daily diluting cultures in increasing concentrations of benzoic acid (up to 20 mM) buffered at external pH 6.5, a pH at which perm...

journal_title：Applied and environmental microbiology

authors： Creamer KE,Ditmars FS,Basting PJ,Kunka KS,Hamdallah IN,Bush SP,Scott Z,He A,Penix SR,Gonzales AS,Eder EK,Camperchioli DW,Berndt A,Clark MW,Rouhier KA,Slonczewski JL

更新日期：2016-12-30 00:00:00

abstract：:Listeria monocytogenes does not produce siderophores for iron acquisition. We demonstrate that a number of microbial siderophores and natural iron-binding compounds are able to promote the growth of iron-starved L. monocytogenes. We suggest that the ability of L. monocytogenes to use a variety of exogenous siderophore...

journal_title：Applied and environmental microbiology

authors： Simon N,Coulanges V,Andre P,Vidon DJ

更新日期：1995-04-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli O157:H7 has a natural reservoir in the intestinal tracts of cattle. Colonization is asymptomatic and transient, but it is not clear if protective immunity is induced. This study demonstrates that prior colonization induces humoral immune responses to bacterial antigens and reduces ba...

journal_title：Applied and environmental microbiology

authors： Naylor SW,Flockhart A,Nart P,Smith DG,Huntley J,Gally DL,Low JC

更新日期：2007-06-01 00:00:00

abstract：:The importance of hydrogenase activity to corrosion of steel was assessed by using mixed populations of sulfate-reducing bacteria isolated from corroded and noncorroded oil pipelines. Biofilms which developed on the steel studs contained detectable numbers of sulfate-reducing bacteria (10 increasing to 10/0.5 cm). How...

journal_title：Applied and environmental microbiology

authors： Bryant RD,Jansen W,Boivin J,Laishley EJ,Costerton JW

更新日期：1991-10-01 00:00:00

abstract：:A collection of 300 isolates of fluorescent pseudomonads was established from Douglas fir-Laccaria bicolor mycorrhizas and mycorrhizosphere and from adjacent bulk soil. These isolates were first phenotypically characterized with the Biolog method. Taxonomic identification assigned 90% of the isolates to the different ...

journal_title：Applied and environmental microbiology

authors： Frey P,Frey-Klett P,Garbaye J,Berge O,Heulin T

更新日期：1997-05-01 00:00:00

abstract：:The rhizobial bacterium Bradyrhizobium japonicum functions as a nitrogen-fixing symbiont of the soybean plant (Glycine max). Plants are capable of producing an oxidative burst, a rapid proliferation of reactive oxygen species (ROS), as a defense mechanism against pathogenic and symbiotic bacteria. Therefore, B. japoni...

journal_title：Applied and environmental microbiology

authors： Donati AJ,Jeon JM,Sangurdekar D,So JS,Chang WS

更新日期：2011-06-01 00:00:00

abstract：:The biological impact of 24-h ("chronic") chromium(VI) [Cr(VI) or chromate] exposure on Shewanella oneidensis MR-1 was assessed by analyzing cellular morphology as well as genome-wide differential gene and protein expression profiles. Cells challenged aerobically with an initial chromate concentration of 0.3 mM in com...

journal_title：Applied and environmental microbiology

authors： Chourey K,Thompson MR,Morrell-Falvey J,Verberkmoes NC,Brown SD,Shah M,Zhou J,Doktycz M,Hettich RL,Thompson DK

更新日期：2006-09-01 00:00:00

abstract：:Rhizobiophage V, isolated from soil in the vicinity of soybean roots, was strongly lytic on Bradyrhizobium japonicum 123B (USDA 123) but only mildly lytic on strain L4-4, a chemically induced small-colony mutant of 123. Numerous bacteriophage-resistant variants were isolated from L4-4 infected with phage V; two were s...

journal_title：Applied and environmental microbiology

authors： Abebe HM,Sadowsky MJ,Kinkle BK,Schmidt EL

更新日期：1992-10-01 00:00:00

abstract：:The utilization of Saccharomyces cerevisiae strains overproducing glycerol and with a reduced ethanol yield is a potentially valuable strategy for producing wine with decreased ethanol content. However, glycerol overproduction is accompanied by acetate accumulation. In this study, we evaluated the effects of the overe...

journal_title：Applied and environmental microbiology

authors： Cambon B,Monteil V,Remize F,Camarasa C,Dequin S

更新日期：2006-07-01 00:00:00

abstract：:Two new nickel-resistant strains of Alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. Alcaligenes xylosoxydans 31A is a heterotrophic bacterium, and Alcaligenes eutrophus KTO2 is an a...

journal_title：Applied and environmental microbiology

authors： Schmidt T,Stoppel RD,Schlegel HG

更新日期：1991-11-01 00:00:00

abstract：:Twenty-seven Shiga toxin-producing Escherichia coli (STEC) strains were isolated from 207 stx-positive French environmental samples. Ten of these strains were positive for stx(1), and 24 were positive for stx(2) (10 were positive for stx(2vh-a) or stx(2vh-b), 19 were positive for stx(2d), and 15 were positive for stx(...

journal_title：Applied and environmental microbiology

authors： Vernozy-Rozand C,Montet MP,Bertin Y,Trably F,Girardeau JP,Martin C,Livrelli V,Beutin L

更新日期：2004-04-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter capsulatus can grow with short- to long-chain fatty acids as the sole carbon source (R. G. Kranz, K. K. Gabbert, T. A. Locke, and M. T. Madigan, Appl. Environ. Microbiol. 63:3003-3009, 1997). Concomitant with growth on fatty acids is the production to high levels of the polyest...

journal_title：Applied and environmental microbiology

authors： Kranz RG,Gabbert KK,Madigan MT

更新日期：1997-08-01 00:00:00

abstract：:The bacterial host range of coliphage P1 was extented by using the heat-inducible phage P1clr100KM. A gene for kanamycin resistance was transferred from Escherichia coli to members of the family Enterobacteriaceae and some other genera of gram-negative bacteria. P1 phage was produced by thermal induction from the lyso...

journal_title：Applied and environmental microbiology

authors： Murooka Y,Harada T

更新日期：1979-10-01 00:00:00

abstract：:We report here a biochemical study of resistance to azole antifungal agents in a field isolate (S-27) of a fungal phytopathogen. Isolates of Septoria tritici were compared in vitro, and their responses reflected that observed in the field, with S-27 exhibiting resistance relative to RL2. In untreated cultures, both RL...

journal_title：Applied and environmental microbiology

authors： Joseph-Horne T,Hollomon D,Manning N,Kelly SL

更新日期：1996-01-01 00:00:00