Positive selection systems for discovery of novel polyester biosynthesis genes based on fatty acid detoxification.

abstract：:The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface...

journal_title：Applied and environmental microbiology

authors： Kaneko T,Holder-Franklin M,Franklin M

更新日期：1977-04-01 00:00:00

abstract：:A simple, efficient procedure for removing lactic acid and for reducing nonprotein nitrogen and ash in lactic acid whey has been developed. The procedure consists of culturing Candida ingens on the whey. This organism could assimilate >98% of the lactic acid and approximately 40% of the nonprotein nitrogen. Ash reduct...

journal_title：Applied and environmental microbiology

authors： Ruiz LP,Gurnsey JC,Short JL

更新日期：1978-04-01 00:00:00

abstract：:The effects of various nitrogen sources and of the cultivation time on the biosynthesis of N-labelled azoverdin were studied in batch cultures of Azomonas macrocytogenes ATCC 12334. Growth for 48 h in the presence of ammonium sulfate (0.2 g/liter) resulted in 95% labelling of azoverdin with N. A growth-related synthes...

journal_title：Applied and environmental microbiology

authors： Dobisová M,Abdallah MA,Kyslík P

更新日期：1994-12-01 00:00:00

abstract：:Ensifer adhaerens is a soil bacterium that attaches to other bacteria and may cause lysis of these other bacteria. Based on the sequence of its small-subunit rRNA gene, E. adhaerens is related to Sinorhizobium spp. E. adhaerens ATCC 33499 did not nodulate Phaseolus vulgaris (bean) or Leucaena leucocephala, but with sy...

journal_title：Applied and environmental microbiology

authors： Rogel MA,Hernández-Lucas I,Kuykendall LD,Balkwill DL,Martinez-Romero E

更新日期：2001-07-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis strain E40 filaments were developed under conditions of a reduced water activity (a(w)) of 0.95 in tryptic soy broth (TSB) or tryptic soy agar (TSA) supplemented with 8% or 7% NaCl, respectively. Filament formation was accompanied by an increase of biomass without an increase in...

journal_title：Applied and environmental microbiology

authors： Stackhouse RR,Faith NG,Kaspar CW,Czuprynski CJ,Wong AC

更新日期：2012-04-01 00:00:00

abstract：:The distribution of Clostridium botulinum serotypes A, B, E, and F in Finnish trout farms was examined. A total of 333 samples were tested with a neurotoxin-specific PCR assay. C. botulinum type E was found in 68% of the farm sediment samples, in 15% of the fish intestinal samples, and in 5% of the fish skin samples. ...

journal_title：Applied and environmental microbiology

authors： Hielm S,Björkroth J,Hyytiä E,Korkeala H

更新日期：1998-11-01 00:00:00

abstract：:In two previous studies dealing with lactic acid bacteria (LAB) from modified-atmosphere-packaged (MAP) broiler products and a broiler processing plant, several isolates remained unidentified. According to 16S rRNA gene sequence analysis, 36 isolates were assigned to the genus Enterococcus. Numerical analysis of combi...

journal_title：Applied and environmental microbiology

authors： Rahkila R,Johansson P,Säde E,Björkroth J

更新日期：2011-02-01 00:00:00

abstract：:The chlorine resistance of cysts of the flagellate protozoan Giardia muris was examined. This organism, which is pathogenic to mice, is being considered as a model for the inactivation of the human pathogen Giardia lamblia. Excystation was used as the criterion for cyst viability. Experiments were performed at pH 5, 7...

journal_title：Applied and environmental microbiology

authors： Leahy JG,Rubin AJ,Sproul OJ

更新日期：1987-07-01 00:00:00

abstract：:Marine viruses are an important component of the microbial food web, influencing microbial diversity and contributing to bacterial mortality rates. Resistance to cooccurring cyanophages has been reported for natural communities of Synechococcus spp.; however, little is known about the nature of this resistance. This s...

journal_title：Applied and environmental microbiology

authors： Stoddard LI,Martiny JB,Marston MF

更新日期：2007-09-01 00:00:00

abstract：:The industrial production of beta-carotene with the zygomycete Blakeslea trispora involves the joint cultivation of mycelia of opposite sex in the presence of beta-ionone and other chemical activators. We have obtained improved strains by mutation and heterokaryosis. We chose wild strains on the basis of their growth ...

journal_title：Applied and environmental microbiology

authors： Mehta BJ,Obraztsova IN,Cerdá-Olmedo E

更新日期：2003-07-01 00:00:00

abstract：:The ability of phages to specifically interact with and lyse their host bacteria makes them ideal antibacterial agents. The range of applications of bacteriophage can be extended by their immobilization on inert surfaces. A novel method for the oriented immobilization of bacteriophage has been developed. The method wa...

journal_title：Applied and environmental microbiology

authors： Anany H,Chen W,Pelton R,Griffiths MW

更新日期：2011-09-01 00:00:00

abstract：:Selectively regulating gene expression is an essential molecular tool that is lacking for many pathogenic gram-positive bacteria. In this report, we describe the evaluation of a series of promoters regulated by the bacteriophage P1 temperature-sensitive C1 repressor in Enterococcus faecium, Enterococcus faecalis, and ...

journal_title：Applied and environmental microbiology

authors： Schofield DA,Westwater C,Hoel BD,Werner PA,Norris JS,Schmidt MG

更新日期：2003-06-01 00:00:00

abstract：:The lantibiotic nisin is produced by several strains of Lactococcus lactis. The complete gene cluster for nisin biosynthesis in L. lactis 6F3 comprises 15 kb of DNA. As described previously, the structural gene nisA is followed by the genes nisB, nisT, nisC, nisI, nisP, nisR, and nisK. Further analysis revealed three ...

journal_title：Applied and environmental microbiology

authors： Siegers K,Entian KD

更新日期：1995-03-01 00:00:00

abstract：:Erysipelothrix rhusiopathiae causes swine erysipelas, an important infectious disease in the swine industry. In Japan, the incidence of acute swine erysipelas due to E. rhusiopathiae serovar 1a has recently increased markedly. To study the genetic relatedness of the strains from the recent cases, we analyzed 34 E. rhu...

journal_title：Applied and environmental microbiology

authors： Ogawa Y,Shiraiwa K,Ogura Y,Ooka T,Nishikawa S,Eguchi M,Hayashi T,Shimoji Y

更新日期：2017-05-17 00:00:00

abstract：:A protein (PhaX) that interacted with poly(3-hydroxybutyrate) (PHB) depolymerase PhaZa1 and with PHB granule-associated phasin protein PhaP2 was identified by two-hybrid analysis. Deletion of phaX resulted in an increase in the level of polyphosphate (polyP) granule formation and in impairment of PHB utilization in nu...

journal_title：Applied and environmental microbiology

authors： Tumlirsch T,Sznajder A,Jendrossek D

更新日期：2015-12-01 00:00:00

abstract：:The successful nodulation of legumes by a Rhizobium strain is determined by the competitive ability of that strain against the mixture of other native and inoculant rhizobia. Competition among six Leucaena rhizobial strains in single and multistrain inoculants were studied. Field inoculation trials were conducted in a...

journal_title：Applied and environmental microbiology

authors： Moawad H,Bohlool BB

更新日期：1984-07-01 00:00:00

abstract：:The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can ...

journal_title：Applied and environmental microbiology

authors： Haarman M,Knol J

更新日期：2006-04-01 00:00:00

abstract：:A method was developed for extraction of DNA from Chroococcidiopsis that overcomes obstacles posed by bacterial contamination and the presence of a thick envelope surrounding the cyanobacterial cells. The method is based on the resistance of Chroococcidiopsis to lysozyme and consists of a lysozyme treatment followed b...

journal_title：Applied and environmental microbiology

authors： Billi D,Grilli Caiola M,Paolozzi L,Ghelardini P

更新日期：1998-10-01 00:00:00

abstract：:Pure nonhydrolyzed inulin was directly converted to ethanol in a simultaneous saccharification and fermentation process. An inulinase-hyperproducing mutant, Aspergillus niger 817, was grown in a submerged culture at 30 degrees C for 5 days. The inulin-digestive liquid culture (150 ml) was supplemented with 45 g of inu...

journal_title：Applied and environmental microbiology

authors： Ohta K,Hamada S,Nakamura T

更新日期：1993-03-01 00:00:00

abstract：:The bifidobacterial and lactobacillus populations of fecal samples collected from 10 human subjects were studied. The numbers of bifidobacteria were similar in the fecal samples of all of the subjects, but lactobacillus numbers varied, even between samples collected from the same individual. Analysis of the compositio...

journal_title：Applied and environmental microbiology

authors： Kimura K,McCartney AL,McConnell MA,Tannock GW

更新日期：1997-09-01 00:00:00

abstract：:Protein disulfide isomerase (PDI) is important in assisting the folding and maturation of secretory proteins in eukaryotes. A gene, pdiA, encoding PDIA was previously isolated from Aspergillus niger, and we report its functional characterization here. Functional analysis of PDIA showed that it catalyzes the refolding ...

journal_title：Applied and environmental microbiology

authors： Ngiam C,Jeenes DJ,Punt PJ,Van Den Hondel CA,Archer DB

更新日期：2000-02-01 00:00:00

abstract：:A real-time PCR assay was developed for the quantitative detection of Campylobacter jejuni in foods after enrichment culture. The specificity of the assay for C. jejuni was demonstrated with a diverse range of Campylobacter species, related organisms, and unrelated genera. The assay had a linear range of quantificatio...

journal_title：Applied and environmental microbiology

authors： Sails AD,Fox AJ,Bolton FJ,Wareing DR,Greenway DL

更新日期：2003-03-01 00:00:00

abstract：:Of 98 suspected food-borne Clostridium perfringens isolates obtained from a nationwide survey by the Food and Consumer Product Safety Authority in The Netherlands, 59 strains were identified as C. perfringens type A. Using PCR-based techniques, the cpe gene encoding enterotoxin was detected in eight isolates, showing ...

journal_title：Applied and environmental microbiology

authors： Xiao Y,Wagendorp A,Moezelaar R,Abee T,Wells-Bennik MH

更新日期：2012-10-01 00:00:00

abstract：:A physiological, unbalanced model is presented that explicitly describes growth of the marine cyanobacterium Trichodesmium sp. at the expense of N(2) (diazotrophy). The model involves the dynamics of intracellular reserves of carbon and nitrogen and allows the uncoupling of the metabolism of these elements. The result...

journal_title：Applied and environmental microbiology

authors： Rabouille S,Staal M,Stal LJ,Soetaert K

更新日期：2006-05-01 00:00:00

abstract：:Rotavirus SA11, suspended in tryptose phosphate broth with 2.5 mg of rhodamine B per ml, was aerosolized (Collison nebulizer) into a rotating drum, and the aerosols were held at 20 +/- 1 degree C with the desired relative humidity (RH). An all-glass impinger with tryptose phosphate broth was used to collect 1-min (5.6...

journal_title：Applied and environmental microbiology

authors： Sattar SA,Ijaz MK,Johnson-Lussenburg CM,Springthorpe VS

更新日期：1984-04-01 00:00:00

abstract：:A biofilm-forming marine bacterium, D2, isolated from the surface of the tunicate Ciona intestinalis, was found to produce a novel, 190-kDa protein with antibacterial activity. The protein contained at least two subunits of 60 and 80 kDa, joined together by noncovalent bonds, and was shown to be released by D2 cells i...

journal_title：Applied and environmental microbiology

authors： James SG,Holmström C,Kjelleberg S

更新日期：1996-08-01 00:00:00

abstract：:We have previously reported that an acid tolerance response (ATR) can be induced in Streptococcus macedonicus cells at mid-log phase after autoacidification, transient exposure to acidic pH, or acid habituation, as well as at stationary phase. Here, we compared the transcriptional profiles of these epigenetic phenotyp...

journal_title：Applied and environmental microbiology

authors： Papadimitriou K,Boutou E,Zoumpopoulou G,Tarantilis PA,Polissiou M,Vorgias CE,Tsakalidou E

更新日期：2008-10-01 00:00:00

abstract：:We analyzed changes in bacterioplankton morphology and composition during enhanced protozoan grazing by image analysis and fluorescent in situ hybridization with group-specific rRNA-targeted oligonucleotide probes. Enclosure experiments were conducted in a small, fishless freshwater pond which was dominated by the cla...

journal_title：Applied and environmental microbiology

authors： Jürgens K,Pernthaler J,Schalla S,Amann R

更新日期：1999-03-01 00:00:00

abstract：:An extracellular H(inf2)O(inf2)-requiring Remazol brilliant blue R (RBBR) decolorizing enzymatic activity was found in the culture medium of Pleurotus ostreatus. The enzymatic activity was maximally obtained in idiophase, and the optimum C/N ratio was 24. High C/N ratios repressed the enzymatic activity, and addition ...

journal_title：Applied and environmental microbiology

authors： Shin K,Oh I,Kim C

更新日期：1997-05-01 00:00:00

abstract：:Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and acidic heathland...

journal_title：Applied and environmental microbiology

authors： Herrmann M,Saunders AM,Schramm A

更新日期：2009-05-01 00:00:00