Formation of polyphosphate by polyphosphate kinases and its relationship to poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha strain H16.

abstract：:Two Baeyer-Villiger monooxygenases (BVMOs), designated BoBVMO and AmBVMO, were discovered from Bradyrhizobium oligotrophicum and Aeromicrobium marinum, respectively. Both monooxygenases displayed novel features for catalyzing the asymmetric sulfoxidation of bulky and pharmaceutically relevant thioethers. Evolutionary ...

journal_title：Applied and environmental microbiology

authors： Zhang Y,Liu F,Xu N,Wu YQ,Zheng YC,Zhao Q,Lin G,Yu HL,Xu JH

更新日期：2018-07-02 00:00:00

abstract：:The antimicrobial activities of sucrose monolaurate and a novel ester, lactose monolaurate (LML), were tested. Gram-positive bacteria were more susceptible than Gram-negative bacteria to both esters. The minimal bactericidal concentrations of LML were 5 to 9.5 mM for Listeria monocytogenes isolates and 0.2 to 2 mM for...

journal_title：Applied and environmental microbiology

authors： Wagh A,Shen S,Shen FA,Miller CD,Walsh MK

更新日期：2012-05-01 00:00:00

abstract：:A novel single-step procedure for the purification of staphylococcal enterotoxin A (SEA), namely, dye ligand affinity chromatography with the triazine dye Red A, was developed. SEA purified by this method produced a single band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The yield from...

journal_title：Applied and environmental microbiology

authors： Reynolds D,Tranter HS,Sage R,Hambleton P

更新日期：1988-07-01 00:00:00

abstract：:The acI lineage of freshwater Actinobacteria is a cosmopolitan and often numerically dominant member of lake bacterial communities. We conducted a survey of acI 16S rRNA genes and 16S-23S rRNA internal transcribed spacer regions from 18 Wisconsin lakes and used standard nonphylogenetic and phylogenetic statistical app...

journal_title：Applied and environmental microbiology

authors： Newton RJ,Jones SE,Helmus MR,McMahon KD

更新日期：2007-11-01 00:00:00

abstract：:The addition of positively charged colloidal materials to the growth medium markedly increased the concentration of cellulase enzymes produced by Trichoderma reesei Rut-C30. Filter paper activities of up to 4 and 13 IU/ml have been achieved by the addition of colloidal materials, using 3% lactose and 3% cellulose, res...

journal_title：Applied and environmental microbiology

authors： Duff SJ,Cooper DG,Fuller OM

更新日期：1985-04-01 00:00:00

abstract：:The pathogenicity of Listeria monocytogenes as influenced by growth on crabmeat at 5 and 10 degrees C was studied. Crabmeat was inoculated with L. monocytogenes V7 (ca. 10(4) CFU/g) and incubated for up to 14 days at 5 and 10 degrees C. At selected incubation times, L. monocytogenes was removed from crabmeat by washin...

journal_title：Applied and environmental microbiology

authors： Brackett RE,Beuchat LR

更新日期：1990-05-01 00:00:00

abstract：:This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology. A central composite design was used as an experimental design ...

journal_title：Applied and environmental microbiology

authors： Oh S,Rheem S,Sim J,Kim S,Baek Y

更新日期：1995-11-01 00:00:00

abstract：:Flavobacteria are a poorly understood and speciated group of commensal bacteria and opportunistic pathogens. The psychrotroph Flavobacterium psychrophilum is the etiological agent of rainbow trout fry syndrome and bacterial cold water disease, septicemic diseases that heavily impact salmonids. Consequently, two verifi...

journal_title：Applied and environmental microbiology

authors： Crump EM,Perry MB,Clouthier SC,Kay WW

更新日期：2001-02-01 00:00:00

abstract：:Consumption of poultry contaminated with Campylobacter jejuni is a risk factor for human gastrointestinal disease. The rational development of control strategies for Campylobacter within chickens requires an understanding of the colonization process at the molecular and population levels, both within and between hosts...

journal_title：Applied and environmental microbiology

authors： Coward C,van Diemen PM,Conlan AJ,Gog JR,Stevens MP,Jones MA,Maskell DJ

更新日期：2008-06-01 00:00:00

abstract：:During malolactic fermentation in wine by Oenococcus oeni, the degradation of citric acid was delayed compared to the degradation of malic acid. The maximum concentration of diacetyl, an intermediary compound in the citric acid metabolism with a buttery or nutty flavor, coincided with the exhaustion of malic acid in t...

journal_title：Applied and environmental microbiology

authors： Nielsen JC,Richelieu M

更新日期：1999-02-01 00:00:00

abstract：:Rapid pathogen detection is crucial for the timely introduction of therapeutics. Two groups (one in the United Kingdom and one in the United States) independently evaluated inhibitor-resistant PCR reagents for the direct testing of substrates. In the United Kingdom, a multiplexed Bacillus anthracis (target) and Bacill...

journal_title：Applied and environmental microbiology

authors： Minogue TD,Rachwal PA,Trombley Hall A,Koehler JW,Weller SA

更新日期：2014-02-01 00:00:00

abstract：:A separation procedure for the analysis of the enzyme components of the hyperthermophilic bacterium Thermotoga maritima involved in cellulose and xylan degradation was developed. Resolution of the enzymes was achieved by a combination of fast protein liquid chromatography anion exchange and hydrophobic interaction chr...

journal_title：Applied and environmental microbiology

authors： Bronnenmeier K,Kern A,Liebl W,Staudenbauer WL

更新日期：1995-04-01 00:00:00

abstract：:The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring t...

journal_title：Applied and environmental microbiology

authors： Li J,Hu X,Yan J,Yuan Z

更新日期：2009-06-01 00:00:00

abstract：:Fungal attacks on stored vegetable and fruits are responsible for losses of products. There is an active research field to develop alternative strategies for postharvest disease management, and the use of biocontrol agents represents a promising approach. Understanding the molecular bases of the biocontrol activity of...

journal_title：Applied and environmental microbiology

authors： Castoria R,Miccoli C,Barone G,Palmieri D,De Curtis F,Lima G,Heitman J,Ianiri G

更新日期：2021-01-15 00:00:00

abstract：:Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatographically at the rat...

journal_title：Applied and environmental microbiology

authors： David KA,Apte SK,Banerji A,Thomas J

更新日期：1980-05-01 00:00:00

abstract：:Unspecific peroxygenases (UPOs) constitute a new family of fungal heme-thiolate enzymes in which there is high biotechnological interest. Although several thousand genes encoding hypothetical UPO-type proteins have been identified in sequenced fungal genomes and other databases, only a few UPO enzymes have been experi...

journal_title：Applied and environmental microbiology

authors： Linde D,Olmedo A,González-Benjumea A,Estévez M,Renau-Mínguez C,Carro J,Fernández-Fueyo E,Gutiérrez A,Martínez AT

更新日期：2020-03-18 00:00:00

abstract：:Tn5 insertion mutants of a soil isolate, Pseudomonas fluorescens Pf0-1, were selected for decreased ability to adhere to quartz sand in a column assay. Three adhesion-deficient mutants that differed in the location of the Tn5 insertion in the chromosome were isolated and compared with the wild-type strain. One mutant,...

journal_title：Applied and environmental microbiology

authors： Deflaun MF,Marshall BM,Kulle EP,Levy SB

更新日期：1994-07-01 00:00:00

abstract：:The actinomycete Streptomyces setonii 75Vi2 demethylates vanillic acid and guaiacol to protocatechuic acid and catechol, respectively, and then metabolizes the products by the beta-ketoadipate pathway. UV spectroscopy showed that this strain could also metabolize veratrole (1,2-dimethoxybenzene). When grown in veratro...

journal_title：Applied and environmental microbiology

authors： Sutherland JB

更新日期：1986-07-01 00:00:00

abstract：:Sludges derived from wastewater treatment are foul-smelling, biologically unstable substances. As well as containing numerous pathogenic microorganisms, they also consist of organic matter that can be used as agricultural fertilizer. Legislation nevertheless requires sludges to be virologically tested prior to spreadi...

journal_title：Applied and environmental microbiology

authors： Monpoeho S,Maul A,Bonnin C,Patria L,Ranarijaona S,Billaudel S,Ferré V

更新日期：2004-09-01 00:00:00

abstract：:Pseudomonas putida KT2440, a saprophytic soil bacterium that colonizes the plant root, is a suitable microorganism for the removal of pollutants and a stable host for foreign genes used in biotransformation processes. Because of its potential use in agriculture and industry, we investigated the conditions for the opti...

journal_title：Applied and environmental microbiology

authors： Muñoz-Rojas J,Bernal P,Duque E,Godoy P,Segura A,Ramos JL

更新日期：2006-01-01 00:00:00

abstract：:Progesterone was converted to 5alpha-pregnane-3alpha-ol-20-one, delta4-pregnene-20alpha-ol-3-one, delta4-pregnene-14alpha-ol-3,20-dione, delta4-pregnene-7beta,14alpha-diol-3,20-dione, and delta4-pregnene-6beta,11alpha-diol-3,20-dione by cell cultures of Lycopersicon esculentum. Cell cultures of Capsicum frutescens (gr...

journal_title：Applied and environmental microbiology

authors： Yagen B,Gallili GE,Mateles RI

更新日期：1978-08-01 00:00:00

abstract：:The sensitivity of two recently isolated antigenic variants of echovirus type 25 (Montpellier 76-1262 and Thionville 86-222) to glutaraldehyde (GTA) at low concentrations was compared with that of the JV-4 prototype strain. The purified viruses were treated under the same conditions with GTA at concentrations ranging ...

journal_title：Applied and environmental microbiology

authors： Chambon M,Bailly JL,Peigue-Lafeuille H

更新日期：1994-02-01 00:00:00

abstract：:A variety of lactic acid bacteria were screened for their ability to produce folate intracellularly and/or extracellularly. Lactococcus lactis, Streptococcus thermophilus, and Leuconostoc spp. all produced folate, while most Lactobacillus spp., with the exception of Lactobacillus plantarum, were not able to produce fo...

journal_title：Applied and environmental microbiology

authors： Sybesma W,Starrenburg M,Tijsseling L,Hoefnagel MH,Hugenholtz J

更新日期：2003-08-01 00:00:00

abstract：:This investigation introduces the use of an immunoglobulin A mouse myeloma protein for the detection of Salmonella spp. in milk. The immunoglobulin A protein M467 reacts with flagellin from a wide variety of serotypes. Two assays were developed which used an enzyme-linked immunosorbent assay (ELISA) and M467. Alkaline...

journal_title：Applied and environmental microbiology

authors： Smith AM,Jones C

更新日期：1983-10-01 00:00:00

abstract：:Laboratory conditions have been identified that cause the rapid death of cultures of cyanobacteria producing urease. Once the death phase had initiated in the stationary growth phase, cells were rapidly bleached of all pigmentation. Null mutations in the ureC gene, encoding the alpha subunit of urease, were constructe...

journal_title：Applied and environmental microbiology

authors： Sakamoto T,Delgaizo VB,Bryant DA

更新日期：1998-07-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity was determined during the growth cycle of unheated and heat-injured cells of Staphylococcus aureus MF-31. SOd activity levels dropped in unheated cells during the lag phase, increased during logarithmic phase, and became constant in the stationary phase. Cells which were sublethally...

journal_title：Applied and environmental microbiology

authors： Bucker ER,Martin SE

更新日期：1981-03-01 00:00:00

abstract：:The consumption of acetate in soils taken from a nutrient gradient in the northern Florida Everglades was studied by stable isotope probing. Bacterial and archaeal 16S rRNA gene clone libraries from eutrophic and oligotrophic soil microcosms strongly suggest that a significant amount of acetate is consumed by syntroph...

journal_title：Applied and environmental microbiology

authors： Chauhan A,Ogram A

更新日期：2006-10-01 00:00:00

abstract：:The practical task of adapting an original potentiometric technique to the bacteriological analysis of water is discussed. Various laboratory strains of organisms belonging to the usual aquatic flora were inoculated one by one in a minimal lactose broth supplied with lipoic (thioctic) acid. The time evolution of the r...

journal_title：Applied and environmental microbiology

authors： Charriere G,Jouenne T,Lemeland JF,Selegny E,Junter GA

更新日期：1984-01-01 00:00:00

abstract：:Information on the interaction between mixed populations in the rumen and plant phenolics is required to fully elucidate the limitations of phenolic compounds on forage digestibility. The objective of this study was to examine the degradation of Italian ryegrass (Lolium multiflorum L.) hay incubated with mixed ruminal...

journal_title：Applied and environmental microbiology

authors： Theodorou MK,Gascoyne DJ,Akin DE,Hartley RD

更新日期：1987-05-01 00:00:00

abstract：:In Lactococcus lactis, which is widely used as a starter in the cheese industry, the first step of aromatic and branched-chain amino acid degradation is a transamination which is catalyzed by two major aminotransferases. We have previously purified and characterized biochemically and genetically the aromatic aminotran...

journal_title：Applied and environmental microbiology

authors： Yvon M,Chambellon E,Bolotin A,Roudot-Algaron F

更新日期：2000-02-01 00:00:00