Abstract:
:A method was developed for extraction of DNA from Chroococcidiopsis that overcomes obstacles posed by bacterial contamination and the presence of a thick envelope surrounding the cyanobacterial cells. The method is based on the resistance of Chroococcidiopsis to lysozyme and consists of a lysozyme treatment followed by osmotic shock that reduces the bacterial contamination by 3 orders of magnitude. Then DNase treatment is performed to eliminate DNA from the bacterial lysate. Lysis of Chroococcidiopsis cells is achieved by grinding with glass beads in the presence of hot phenol. Extracted DNA is further purified by cesium-chloride density gradient ultracentrifugation. This method permitted the first molecular approach to the study of Chroococcidiopsis, and a 570-bp fragment of the gene ftsZ was cloned and sequenced.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Billi D,Grilli Caiola M,Paolozzi L,Ghelardini Pdoi
10.1128/AEM.64.10.4053-4056.1998subject
Has Abstractpub_date
1998-10-01 00:00:00pages
4053-6issue
10eissn
0099-2240issn
1098-5336journal_volume
64pub_type
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