Quantitative real-time PCR analysis of fecal Lactobacillus species in infants receiving a prebiotic infant formula.

Abstract:

:The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can exert beneficial effects on, for example, diarrhea and atopy. However, the Lactobacillus species distribution in breast-fed or formula-fed infants has not yet been determined in great detail. For accurate enumeration of different lactobacilli, duplex 5' nuclease assays, targeted on rRNA intergenic spacer regions, were developed for Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus reuteri, and Lactobacillus rhamnosus. The designed and validated assays were used to determine the amounts of different Lactobacillus species in fecal samples of infants receiving a standard formula (SF) or a standard formula supplemented with galacto- and fructo-oligosaccharides in a 9:1 ratio (OSF). A breast-fed group (BF) was studied in parallel as a reference. During the 6-week intervention period a significant increase was shown in total percentage of fecal lactobacilli in the BF group (0.8% +/- 0.3% versus 4.1% +/- 1.5%) and the OSF group (0.8% +/- 0.3% versus 4.4% +/- 1.4%). The Lactobacillus species distribution in the OSF group was comparable to breast-fed infants, with relatively high levels of L. acidophilus, L. paracasei, and L. casei. The SF-fed infants, on the other hand, contained more L. delbrueckii and less L. paracasei compared to breast-fed infants and OSF-fed infants. An infant milk formula containing a specific mixture of prebiotics is able to induce a microbiota that closely resembles the microbiota of BF infants.

journal_name

Appl Environ Microbiol

authors

Haarman M,Knol J

doi

10.1128/AEM.72.4.2359-2365.2006

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

2359-65

issue

4

eissn

0099-2240

issn

1098-5336

pii

72/4/2359

journal_volume

72

pub_type

杂志文章
  • Development of monoclonal antibodies that identify Vibrio species commonly isolated from infections of humans, fish, and shellfish.

    abstract::Monoclonal antibodies (MAbs) against Vibrio species that infect humans, fish, and shellfish were developed for application in rapid identifications. The pathogens included Vibrio alginolyticus, V. anguillarum, V. carchariae, V. cholerae, V. damsela, V. furnissii, V. harveyi, V. ordalii, V. parahaemolyticus, and V. vul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.11.3694-3700.1992

    authors: Chen D,Hanna PJ,Altmann K,Smith A,Moon P,Hammond LS

    更新日期:1992-11-01 00:00:00

  • Lecithin agar for detection of microbial phospholipases.

    abstract::Lecithin agar was developed on which phospholipase C produced turbid zones and phospholipase A produced clear zones. Reactions on lecithin agar agreed 74% of the time with reactions in egg yolk broth. On lecithin agar, interpretation was easier, phospholipase A was detectable, and opaque zones were visible 1 or 2 days...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.5.784-786.1976

    authors: Chrisope GL,Fox CW,Marshall RT

    更新日期:1976-05-01 00:00:00

  • Computer-assisted identification of anaerobic bacteria.

    abstract::A computer program was developed to identify anaerobic bacteria by using simultaneous pattern recognition via a Bayesian probabilistic model. The system is intended for use as a rapid, precise, and reproducible aid in the identification of unknown isolates. The program operates on a data base of 28 genera comprising 2...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.3.507-511.1978

    authors: Kelley RW,Kellogg ST

    更新日期:1978-03-01 00:00:00

  • Molecular epidemiology of Salmonella enterica serovar typhimurium isolates from cattle in hokkaido, Japan: evidence of clonal replacement and characterization of the disseminated clone.

    abstract::The molecular epidemiology of 545 Salmonella enterica serovar Typhimurium isolates collected between 1977 and 2009 from cattle in Hokkaido, Japan, was investigated using pulsed-field gel electrophoresis (PFGE). Nine main clusters were identified from 116 PFGE patterns. Cluster I comprised 248 isolates, 243 of which po...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01910-10

    authors: Tamamura Y,Uchida I,Tanaka K,Okazaki H,Tezuka S,Hanyu H,Kataoka N,Makino S,Kishima M,Kubota T,Kanno T,Hatama S,Ishihara R,Hata E,Yamada H,Nakaoka Y,Akiba M

    更新日期:2011-03-01 00:00:00

  • Root hair deformation, bacterial attachment, and plant growth in wheat-azospirillum associations.

    abstract::Seven Azospirillum strains induced more deformation of root hairs of wheat than did strains of Rhizobium leguminosarum, Azotobacter chroococcum, or Escherichia coli. Azospirillum sp. strain Sp245 caused the most deformation. Strain Sp245 (isolated from surface sterile roots of wheat) and strain Sp7 (isolated from the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.6.1208-1213.1984

    authors: Jain DK,Patriquin DG

    更新日期:1984-12-01 00:00:00

  • Regulation of cell division, biofilm formation, and virulence by FlhC in Escherichia coli O157:H7 grown on meat.

    abstract::To understand the continuous problems that Escherichia coli O157:H7 causes as food pathogen, this study assessed global gene regulation in bacteria growing on meat. Since FlhD/FlhC of E. coli K-12 laboratory strains was previously established as a major control point in transducing signals from the environment to seve...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00069-11

    authors: Sule P,Horne SM,Logue CM,Prüss BM

    更新日期:2011-06-01 00:00:00

  • Nitrogen control of atrazine utilization in Pseudomonas sp. strain ADP.

    abstract::Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that supp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.12.6987-6993.2003

    authors: García-González V,Govantes F,Shaw LJ,Burns RG,Santero E

    更新日期:2003-12-01 00:00:00

  • Characterization of DC1, a broad-host-range Bcep22-like podovirus.

    abstract::Bcep22-like phages are a recently described group of podoviruses that infect strains of Burkholderia cenocepacia. We have isolated and characterized a novel member of this group named DC1. This podovirus shows many genomic similarities to BcepIL02 and Bcep22, but it infects strains belonging to multiple Burkholderia c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07097-11

    authors: Lynch KH,Stothard P,Dennis JJ

    更新日期:2012-02-01 00:00:00

  • The motility symbiont of the termite gut flagellate Caduceia versatilis is a member of the "Synergistes" group.

    abstract::The flagellate Caduceia versatilis in the gut of the termite Cryptotermes cavifrons reportedly propels itself not by its own flagella but solely by the flagella of ectosymbiotic bacteria. Previous microscopic observations have revealed that the motility symbionts are flagellated rods partially embedded in the host cel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00750-07

    authors: Hongoh Y,Sato T,Dolan MF,Noda S,Ui S,Kudo T,Ohkuma M

    更新日期:2007-10-01 00:00:00

  • Identification of the gene encoding the major cellobiohydrolase of the white rot fungus Phanerochaete chrysosporium.

    abstract::Previous studies have shown that the cellobiohydrolases of the white rot basidiomycete Phanerochaete chrysosporium are encoded by a family of structurally related genes. In this investigation, we identified and sequenced the most highly transcribed gene, cbh1-4. Evidence suggests that in this fungus the dominant isozy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.10.3492-3494.1993

    authors: Vanden Wymelenberg A,Covert S,Cullen D

    更新日期:1993-10-01 00:00:00

  • Detection of hepatitis A virus by hybridization with single-stranded RNA probes.

    abstract::An improved method of dot-blot hybridization to detect hepatitis A virus (HAV) was developed with single-stranded RNA (ssRNA) probes. Radioactive and nonradioactive ssRNA probes were generated by in vitro transcription of HAV templates inserted into the plasmid pGEM-1. 32P-labeled ssRNA probes were at least eightfold ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.10.2487-2495.1987

    authors: Jiang X,Estes MK,Metcalf TG

    更新日期:1987-10-01 00:00:00

  • Effects of 2-bromoethanesulfonic Acid and 2- chloroethanesulfonic Acid on acetate utilization in a continuous-flow methanogenic fixed-film column.

    abstract::2-Bromoethanesulfonic acid (BESA) and 2-chloroethanesulfonic acid (CESA) have been reported to be potent inhibitors of methane formation during methanogenic decomposition in batch cultures. However, in a laboratory-scale continuous-flow methanogenic fixed-film column containing a predominance of acetate-decarboxylatin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.4.1408-1410.1983

    authors: Bouwer EJ,McCarty PL

    更新日期:1983-04-01 00:00:00

  • Analysis of the hupSL operon of the nonheterocystous cyanobacterium Lyngbya majuscula CCAP 1446/4: regulation of transcription and expression under a light-dark regimen.

    abstract::This work presents the characterization of an uptake hydrogenase from a marine filamentous nonheterocystous cyanobacterium, Lyngbya majuscula CCAP 1446/4. The structural genes encoding the uptake hydrogenase (hupSL) were isolated and characterized, and regulatory sequences were identified upstream of hupS. In silico a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4567-4576.2005

    authors: Leitão E,Oxelfelt F,Oliveira P,Moradas-Ferreira P,Tamagnini P

    更新日期:2005-08-01 00:00:00

  • Marker rescue studies of the transfer of recombinant DNA to Streptococcus gordonii in vitro, in foods and gnotobiotic rats.

    abstract::A plasmid marker rescue system based on restoration of the nptII gene was established in Streptococcus gordonii to study the transfer of bacterial and transgenic plant DNA by transformation. In vitro studies revealed that the marker rescue efficiency depends on the type of donor DNA. Plasmid and chromosomal DNA of bac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.10.6121-6127.2003

    authors: Kharazmi M,Sczesny S,Blaut M,Hammes WP,Hertel C

    更新日期:2003-10-01 00:00:00

  • Life, death, and in-between: meanings and methods in microbiology.

    abstract::Determination of microbial viability by the plate count method is routine in microbiology laboratories worldwide. However, limitations of the technique, particularly with respect to environmental microorganisms, are widely recognized. Many alternatives based upon viability staining have been proposed, and these are of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/AEM.00744-11

    authors: Davey HM

    更新日期:2011-08-15 00:00:00

  • Effect of temperature and light on growth of and photosynthesis by Synechococcus isolates typical of those predominating in the octopus spring microbial mat community of Yellowstone National Park.

    abstract::Previous molecular analysis of the Octopus Spring cyanobacterial mat revealed numerous genetically distinct 16S rRNA sequences from predominant Synechococcus populations distantly related to the readily cultivated unicellular cyanobacterium Synechococcus lividus. Patterns in genotype distribution relative to temperatu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.544-550.2006

    authors: Allewalt JP,Bateson MM,Revsbech NP,Slack K,Ward DM

    更新日期:2006-01-01 00:00:00

  • Distributions of Fecal Markers in Wastewater from Different Climatic Zones for Human Fecal Pollution Tracking in Australian Surface Waters.

    abstract::Recreational and potable water supplies polluted with human wastewater can pose a direct health risk to humans. Therefore, sensitive detection of human fecal pollution in environmental waters is very important to water quality authorities around the globe. Microbial source tracking (MST) utilizes human fecal markers (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03765-15

    authors: Ahmed W,Sidhu JPS,Smith K,Beale DJ,Gyawali P,Toze S

    更新日期:2015-12-18 00:00:00

  • Lactobacillus bulgaricus proteinase expressed in Lactococcus lactis is a powerful carrier for cell wall-associated and secreted bovine beta-lactoglobulin fusion proteins.

    abstract::Lactic acid bacteria have a good potential as agents for the delivery of heterologous proteins to the gastrointestinal mucosa and thus for the reequilibration of inappropriate immune responses to food antigens. Bovine beta-lactoglobulin (BLG) is considered a major allergen in cow's milk allergy. We have designed recom...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.6.2917-2923.2002

    authors: Bernasconi E,Germond JE,Delley M,Fritsché R,Corthésy B

    更新日期:2002-06-01 00:00:00

  • 5-keto-D-gluconate production is catalyzed by a quinoprotein glycerol dehydrogenase, major polyol dehydrogenase, in gluconobacter species.

    abstract::Acetic acid bacteria, especially Gluconobacter species, have been known to catalyze the extensive oxidation of sugar alcohols (polyols) such as D-mannitol, glycerol, D-sorbitol, and so on. Gluconobacter species also oxidize sugars and sugar acids and uniquely accumulate two different keto-D-gluconates, 2-keto-D-glucon...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.4.1959-1966.2003

    authors: Matsushita K,Fujii Y,Ano Y,Toyama H,Shinjoh M,Tomiyama N,Miyazaki T,Sugisawa T,Hoshino T,Adachi O

    更新日期:2003-04-01 00:00:00

  • Mapping of genomic regions (quantitative trait loci) controlling production and quality in industrial cultures of the edible basidiomycete Pleurotus ostreatus.

    abstract::Industrial production of the edible basidiomycete Pleurotus ostreatus (oyster mushroom) is based on a solid fermentation process in which a limited number of selected strains are used. Optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.6.3617-3625.2003

    authors: Larraya LM,Alfonso M,Pisabarro AG,Ramírez L

    更新日期:2003-06-01 00:00:00

  • Evaluation of MK filters for recovery of enteroviruses from tap water.

    abstract::The MK filter is an electropositively charged filter that can be used to concentrate enteroviruses from large volumes (400 to 1,000 liters) of water. This filter is less expensive than the commonly used 1MDS electropositive filter. In this study, we compared the recovery of poliovirus 1 (PV1) and that of coxsackieviru...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.6.1974-1977.1994

    authors: Ma JF,Naranjo J,Gerba CP

    更新日期:1994-06-01 00:00:00

  • Nonfermentative thermoalkaliphilic growth is restricted to alkaline environments.

    abstract::Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a n...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01639-09

    authors: McMillan DG,Keis S,Berney M,Cook GM

    更新日期:2009-12-01 00:00:00

  • Delignification of Wood Chips and Pulps by Using Natural and Synthetic Porphyrins: Models of Fungal Decay.

    abstract::Kraft pulps, prepared from softwoods, and small chips of birch wood were treated with heme and tert-butyl hydroperoxide in aqueous solutions at reflux temperature. Analyses of treated pulps showed decreases in kappa number (a measure of lignin content) from about 36 to less than 2, with concomitant increases in bright...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.1.62-68.1988

    authors: Paszczyński A,Crawford RL,Blanchette RA

    更新日期:1988-01-01 00:00:00

  • Metabolic pathway involved in 2-methyl-6-ethylaniline degradation by Sphingobium sp. strain MEA3-1 and cloning of the novel flavin-dependent monooxygenase system meaBA.

    abstract::2-Methyl-6-ethylaniline (MEA) is the main microbial degradation intermediate of the chloroacetanilide herbicides acetochlor and metolachlor. Sphingobium sp. strain MEA3-1 can utilize MEA and various alkyl-substituted aniline and phenol compounds as sole carbon and energy sources for growth. We isolated the mutant stra...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01883-15

    authors: Dong W,Chen Q,Hou Y,Li S,Zhuang K,Huang F,Zhou J,Li Z,Wang J,Fu L,Zhang Z,Huang Y,Wang F,Cui Z

    更新日期:2015-12-01 00:00:00

  • Formation of polyphosphate by polyphosphate kinases and its relationship to poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha strain H16.

    abstract::A protein (PhaX) that interacted with poly(3-hydroxybutyrate) (PHB) depolymerase PhaZa1 and with PHB granule-associated phasin protein PhaP2 was identified by two-hybrid analysis. Deletion of phaX resulted in an increase in the level of polyphosphate (polyP) granule formation and in impairment of PHB utilization in nu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02279-15

    authors: Tumlirsch T,Sznajder A,Jendrossek D

    更新日期:2015-12-01 00:00:00

  • Production and properties of cyanobacterial endotoxins.

    abstract::Lipopolysaccharides (LPS) were isolated from four species of cyanobacteria (Anabaena flos-aquae UTEX 1444. A. cylindrica, Oscillatoria tenuis, and O. brevis) frequently occurring in drinking-water supplies. The cyanobacterial LPS contained glucose, xylose, mannose, and rhamnose, but differed from the LPS derived from ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.1.104-109.1982

    authors: Keleti G,Sykora JL

    更新日期:1982-01-01 00:00:00

  • Transgalactosylation in a water-solvent biphasic reaction system with beta-galactosidase displayed on the surfaces of Bacillus subtilis spores.

    abstract::The ever-increasing industrial demand for biocatalysis necessitates innovations in the preparation and stabilization of biocatalysts. In this study, we demonstrated that beta-galactosidase (beta-Gal) displayed on Bacillus spores by fusion to the spore coat proteins (CotG) may be used as a whole-cell immobilized biocat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01489-06

    authors: Kwon SJ,Jung HC,Pan JG

    更新日期:2007-04-01 00:00:00

  • Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene.

    abstract::Poplar, a plant species frequently used for phytoremediation of groundwater contaminated with organic solvents, was inoculated with the endophyte Burkholderia cepacia VM1468. This strain, whose natural host is yellow lupine, contains the pTOM-Bu61 plasmid coding for constitutively expressed toluene degradation. Nonino...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8500-8505.2005

    authors: Taghavi S,Barac T,Greenberg B,Borremans B,Vangronsveld J,van der Lelie D

    更新日期:2005-12-01 00:00:00

  • Bartonella chomelii is the most frequent species infecting cattle grazing in communal mountain pastures in Spain.

    abstract::The presence of Bartonella spp. was investigated in domestic ungulates grazing in communal pastures from a mountain area in northern Spain, where 18.3% (17/93) of cattle were found to be positive by PCR combined with a reverse line blot (PCR/RLB), whereas sheep (n = 133) or horses (n = 91) were found not to be infecte...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03159-14

    authors: Antequera-Gómez ML,Lozano-Almendral L,Barandika JF,González-Martín-Niño RM,Rodríguez-Moreno I,García-Pérez AL,Gil H

    更新日期:2015-01-01 00:00:00

  • Isolation of a bacterial strain with the ability to utilize the sulfonated azo compound 4-carboxy-4'-sulfoazobenzene as the sole source of carbon and energy.

    abstract::A bacterial strain (strain S5) which grows aerobically with the sulfonated azo compound 4-carboxy-4'-sulfoazobenzene as the sole source of carbon and energy was isolated. This strain was obtained by continuous adaptation of "Hydrogenophaga palleronii" S1, which has the ability to grow aerobically with 4-aminobenzenesu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.6.2315-2317.1998

    authors: Blümel S,Contzen M,Lutz M,Stolz A,Knackmuss HJ

    更新日期:1998-06-01 00:00:00