Lactobacillus bulgaricus proteinase expressed in Lactococcus lactis is a powerful carrier for cell wall-associated and secreted bovine beta-lactoglobulin fusion proteins.

abstract：:Very little is known about the interaction of bryophytes with bacteria. Therefore, we analyzed bacteria associated with three bryophyte species, Tortula ruralis, Aulacomnium palustre, and Sphagnum rubellum, which represent typical moss species of three nutrient-poor plant communities at the southern Baltic Sea coast i...

journal_title：Applied and environmental microbiology

authors： Opelt K,Berg G

更新日期：2004-11-01 00:00:00

abstract：:Relationships between the hyphae of Agaricus bisporus (Lang) Sing and bacteria from the mushroom bed casing layer were examined with a scanning electron microscope. Hyphae growing in the casing layer differed morphologically from compost-grown hyphae. Whereas the compost contained thin single hyphae surrounded by calc...

journal_title：Applied and environmental microbiology

authors： Masaphy S,Levanon D,Tchelet R,Henis Y

更新日期：1987-05-01 00:00:00

abstract：:A study was conducted to determine the influence of arbuscular mycorrhizal (AM) fungi on Salmonella and enterohemorrhagic Escherichia coli O157:H7 (EHEC) in autoclaved soil and translocation into leek plants. Six-week-old leek plants (with [Myc+] or without [Myc-] AM fungi) were inoculated with composite suspensions o...

journal_title：Applied and environmental microbiology

authors： Gurtler JB,Douds DD Jr,Dirks BP,Quinlan JJ,Nicholson AM,Phillips JG,Niemira BA

更新日期：2013-03-01 00:00:00

abstract：:The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an...

journal_title：Applied and environmental microbiology

authors： Gagnevin L,Leach JE,Pruvost O

更新日期：1997-01-01 00:00:00

abstract：:Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV...

journal_title：Applied and environmental microbiology

authors： Morick D,Faigenbaum O,Smirnov M,Fellig Y,Inbal A,Kotler M

更新日期：2015-05-15 00:00:00

abstract：:Vibrio tubiashii is a recently reemerging pathogen of larval bivalve mollusks, causing both toxigenic and invasive disease. Marine Vibrio spp. produce an array of extracellular products as potential pathogenicity factors. Culture supernatants of V. tubiashii have been shown to be toxic to oyster larvae and were report...

journal_title：Applied and environmental microbiology

authors： Hasegawa H,Lind EJ,Boin MA,Häse CC

更新日期：2008-07-01 00:00:00

abstract：:Five media, formulated from the seeds of five legume varieties, dried cow blood, and mineral salts, were assessed for the growth and production of insecticidal properties of Bacillus thuringiensis subsp. israelensis. Bacterial powders prepared from the broth cultures were assayed against the larvae of Aedes aegypti, C...

journal_title：Applied and environmental microbiology

authors： Obeta JA,Okafor N

更新日期：1984-04-01 00:00:00

abstract：:The cry19A operon of Bacillus thuringiensis subsp. jegathesan encodes two proteins, mosquitocidal Cry19A (ORF1; 75 kDa) and an ORF2 (60 kDa) of unknown function. Expression of the cry19A operon in an acrystalliferous strain of B. thuringiensis (4Q7) yielded one small crystal per cell, whereas no crystals were produced...

journal_title：Applied and environmental microbiology

authors： Barboza-Corona JE,Park HW,Bideshi DK,Federici BA

更新日期：2012-03-01 00:00:00

abstract：:Pectin is a complex uronic acid-containing polysaccharide typically found in plant cell walls, though forms of pectin are also found in marine diatoms and seagrasses. Genetic loci that target pectin have recently been identified in two phyla of marine bacteria. These loci appear to encode a pectin saccharification pat...

journal_title：Applied and environmental microbiology

authors： Hobbs JK,Hettle AG,Vickers C,Boraston AB

更新日期：2018-12-13 00:00:00

abstract：:CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The Methanosarcina acetivorans CRISPR-Cas9 system was repurposed by replacing Cas9 with the ca...

journal_title：Applied and environmental microbiology

authors： Dhamad AE,Lessner DJ

更新日期：2020-10-15 00:00:00

abstract：:Iron-oxidizing thiobacilli were adapted to grow on a chalcopyrite and a galena ore concentrate. When grown on the chalcopyrite concentrate, the bacteria exhibited a doubling time of 38.4 +/- 2.9 h, with a final cellular protein concentration of 185 mug/ml and solubilization of 10.3 g of copper per liter. When grown on...

journal_title：Applied and environmental microbiology

authors： Kingma JG,Silver M

更新日期：1980-03-01 00:00:00

abstract：:Phormium yellow leaf (PYL) phytoplasma causes a lethal disease of the monocotyledon, New Zealand flax (Phormium tenax). The 16S rRNA genes of PYL phytoplasma were amplified from infected flax by PCR and cloned, and the nucleotide sequences were determined. DNA sequencing and Southern hybridization analysis of genomic ...

journal_title：Applied and environmental microbiology

authors： Liefting LW,Andersen MT,Beever RE,Gardner RC,Forster RL

更新日期：1996-09-01 00:00:00

abstract：:Vibrio cholerae identification based on molecular sequence data has been hampered by a lack of sequence variation from the closely related Vibrio mimicus. The two species share many genes coding for proteins, such as ctxAB, and show almost identical 16S DNA coding for rRNA (rDNA) sequences. Primers targeting conserved...

journal_title：Applied and environmental microbiology

authors： Chun J,Huq A,Colwell RR

更新日期：1999-05-01 00:00:00

abstract：:Pyrobaculum islandicum uses iron, thiosulfate, and elemental sulfur for anaerobic respiration, while Pyrobaculum aerophilum uses iron and nitrate; however, the constraints on these processes and their physiological mechanisms for iron and sulfur reduction are not well understood. Growth rates on sulfur compounds are h...

journal_title：Applied and environmental microbiology

authors： Feinberg LF,Srikanth R,Vachet RW,Holden JF

更新日期：2008-01-01 00:00:00

abstract：:We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, s...

journal_title：Applied and environmental microbiology

authors： Rollins DM,Coolbaugh JC,Walker RI,Weiss E

更新日期：1983-01-01 00:00:00

abstract：:The isophthalate (IPA) degradation gene cluster (iphACBDR) responsible for the conversion of IPA into protocatechuate (PCA) was isolated from Comamonas sp. strain E6, which utilizes phthalate isomers as sole carbon and energy sources via the PCA 4,5-cleavage pathway. Based on amino acid sequence similarity, the iphA, ...

journal_title：Applied and environmental microbiology

authors： Fukuhara Y,Inakazu K,Kodama N,Kamimura N,Kasai D,Katayama Y,Fukuda M,Masai E

更新日期：2010-01-01 00:00:00

abstract：:The production of microcystins (MC) from Microcystis aeruginosa UTEX 2388 was investigated in a P-limited continuous culture. MC (MC-LR, MC-RR, and MC-YR) from lyophilized M. aeruginosa were extracted with 5% acetic acid, purified by a Sep-Pak C(18) cartridge, and then analyzed by high-performance liquid chromatograph...

journal_title：Applied and environmental microbiology

authors： Oh HM,Lee SJ,Jang MH,Yoon BD

更新日期：2000-01-01 00:00:00

abstract：:Smectite clay minerals are abundant in soils and sediments worldwide and are typically rich in Fe. While recent investigations have shown that the structural Fe(III) bound in clay minerals is reduced by microorganisms, previous studies have not tested growth with clay minerals as the sole electron acceptor. Here we ha...

journal_title：Applied and environmental microbiology

authors： Kostka JE,Dalton DD,Skelton H,Dollhopf S,Stucki JW

更新日期：2002-12-01 00:00:00

abstract：:The vaginal mucosa of ovariectomized female rats has been examined by scanning electron microscopy before and after estrogen [1,3,5(10)-estratriene-3,17beta-diol] treatment. Without estrogen stimulation vaginal colonization is minimal and the epithelium is characterized by a layer of epithelial cells covered with smal...

journal_title：Applied and environmental microbiology

authors： Larsen B,Markovetz AJ,Galask RP

更新日期：1977-07-01 00:00:00

abstract：:The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was clos...

journal_title：Applied and environmental microbiology

authors： Dedysh SN,Pankratov TA,Belova SE,Kulichevskaya IS,Liesack W

更新日期：2006-03-01 00:00:00

abstract：:Phylogenetic and "fingerprinting" analyses of the 16S rRNA genes of prokaryotes have been a mainstay of microbial ecology during the last two decades. However, many methods and results from studies that rely on the 16S rRNA gene for detection and quantification of specific microbial taxa have seemingly received only c...

journal_title：Applied and environmental microbiology

authors： Morales SE,Holben WE

更新日期：2009-05-01 00:00:00

abstract：:The bifidobacterial and lactobacillus populations of fecal samples collected from two human subjects during a 12-month period were studied. The total numbers of bifidobacteria were stable throughout the study period in both subjects, but lactobacillus numbers were less constant. Analysis of the composition of the bifi...

journal_title：Applied and environmental microbiology

authors： McCartney AL,Wenzhi W,Tannock GW

更新日期：1996-12-01 00:00:00

abstract：:Samples of a sandy loam soil taken from a long-term liming experiment in southeast England were amended with solutions of metal sulfate salts. Soils with a range of pHs were amended to contain Cu, Cd, or Zn at concentrations around the maximum permissible values for these metals in agricultural land receiving sewage s...

journal_title：Applied and environmental microbiology

authors： Knight BP,McGrath SP,Chaudri AM

更新日期：1997-01-01 00:00:00

abstract：:A large, obligately anaerobic spirochete (strain PB) was isolated from bovine rumen fluid by a procedure involving rifampin as a selective agent. The helical cells measured 0.6 to 0.7 micron by 12 to 20 micron and possessed approximately 16 periplasmic flagella inserted near each end of the protoplasmic cylinder. The ...

journal_title：Applied and environmental microbiology

authors： Paster BJ,Canale-Parola E

更新日期：1985-08-01 00:00:00

abstract：:Human norovirus (NoV) is the leading cause of nonbacterial acute gastroenteritis epidemics worldwide. High-pressure processing (HPP) has been considered a promising nonthermal processing technology to inactivate food- and waterborne viral pathogens. Due to the lack of an effective cell culture method for human NoV, th...

journal_title：Applied and environmental microbiology

authors： Lou F,Huang P,Neetoo H,Gurtler JB,Niemira BA,Chen H,Jiang X,Li J

更新日期：2012-08-01 00:00:00

abstract：:Cultures of Anabaena variabilis were exposed to different light intensities, and the time course of photoadaptation was measured by photosynthetic rate and changes in pigmentation. A shift down in intensity of 284 muEin . m . sec caused a temporary decrease in the photosynthetic response followed by gradual adaptation...

journal_title：Applied and environmental microbiology

authors： Collins CD,Boylen CW

更新日期：1982-07-01 00:00:00

abstract：:Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain v...

journal_title：Applied and environmental microbiology

authors： Begley M,Cotter PD,Hill C,Ross RP

更新日期：2010-10-01 00:00:00

abstract：:Diverse nifH and nifH-like gene sequences were obtained from the deep-sea surface sediments of the methane hydrate-bearing Okhotsk Sea. Some sequences formed novel families of the NifH or NifH-like proteins, of currently unresolved bacterial or archaeal origin. Comparison with other marine environments indicates envir...

journal_title：Applied and environmental microbiology

authors： Dang H,Luan X,Zhao J,Li J

更新日期：2009-04-01 00:00:00

abstract：:Cyanase catalyzes the decomposition of cyanate into CO(2) and ammonium, with carbamate as an unstable intermediate. The cyanase of Pseudomonas pseudoalcaligenes CECT5344 was negatively regulated by ammonium and positively regulated by cyanate, cyanide, and some cyanometallic complexes. Cyanase activity was not detecte...

journal_title：Applied and environmental microbiology

authors： Luque-Almagro VM,Huertas MJ,Sáez LP,Luque-Romero MM,Moreno-Vivián C,Castillo F,Roldán MD,Blasco R

更新日期：2008-10-01 00:00:00

abstract：:To produce high levels of β-lactams, the filamentous fungus Penicillium rubens (previously named Penicillium chrysogenum) has been subjected to an extensive classical strain improvement (CSI) program during the last few decades. This has led to the accumulation of many mutations that were spread over the genome. Detai...

journal_title：Applied and environmental microbiology

authors： Wu M,Crismaru CG,Salo O,Bovenberg RAL,Driessen AJM

更新日期：2020-01-21 00:00:00