Sequence heterogeneity in the two 16S rRNA genes of Phormium yellow leaf phytoplasma.

abstract：:In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...

journal_title：Applied and environmental microbiology

authors： Huang J,Sonnleitner E,Ren B,Xu Y,Haas D

更新日期：2012-07-01 00:00:00

abstract：:In Pseudomonas fluorescens CHA0, mutation of the GacA-controlled aprA gene (encoding the major extracellular protease) or the gacA regulatory gene resulted in reduced biocontrol activity against the root-knot nematode Meloidogyne incognita during tomato and soybean infection. Culture supernatants of strain CHA0 inhibi...

journal_title：Applied and environmental microbiology

authors： Siddiqui IA,Haas D,Heeb S

更新日期：2005-09-01 00:00:00

abstract：:Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroet...

journal_title：Applied and environmental microbiology

authors： Zylstra GJ,Wackett LP,Gibson DT

更新日期：1989-12-01 00:00:00

abstract：:Mono Lake is an alkaline hypersaline lake that supports high methane oxidation rates. Retrieved pmoA sequences showed a broad diversity of aerobic methane oxidizers including the type I methanotrophs Methylobacter (the dominant genus), Methylomicrobium, and Methylothermus, and the type II methanotroph Methylocystis. S...

journal_title：Applied and environmental microbiology

authors： Lin JL,Joye SB,Scholten JC,Schäfer H,McDonald IR,Murrell JC

更新日期：2005-10-01 00:00:00

abstract：:Microbacterium thermosphactum requires cysteine, alpha-lipoate, nicotinate, pantothenate, p-aminobenzoate, biotin, and thiamin for aerobic growth in glucose-mineral salts medium. Glucose cannot be replaced by Casamino Acids or acids of the tricarboxylic acid cycle as sole carbon and energy sources. The organism can al...

journal_title：Applied and environmental microbiology

authors： Grau FH

更新日期：1979-11-01 00:00:00

abstract：:A defined medium for Aquaspirillum serpens VHL allows the replacement of the complex media now in use. It was developed by batch culture methods but supports growth in continuous culture. A basal salts medium supplemented with L-aspartic acid, L-alanine, and L-glutamic acid provided the best growth (turbidity), as lon...

journal_title：Applied and environmental microbiology

authors： Whitby GE,Murray RG

更新日期：1980-01-01 00:00:00

abstract：:Aflatoxin B1 in a 5-micrograms/ml water solution was sensitive to ionizing radiation. Inactivation was assayed by the Ames microsome mutagenicity test and confirmed by thin-layer chromatography. Destruction of aflatoxin B1 had already begun at 2.5 kilograms (kGy; 1 kGy = 0.1 Mrad), but a dose exceeding 10 kGy was nece...

journal_title：Applied and environmental microbiology

authors： Van Dyck PJ,Tobback P,Feyes M,van de Voorde H

更新日期：1982-06-01 00:00:00

abstract：:Although there have been several reports on the efficacy assessment of a Salmonella enterica serovar Enteritidis vaccine against intestinal and parenchymatous organ diseases of laying hens, no public health risk characterization of its long-term effect on eggs has been reported. In this study, we attempted to assess t...

journal_title：Applied and environmental microbiology

authors： Toyota-Hanatani Y,Ekawa T,Ohta H,Igimi S,Hara-Kudo Y,Sasai K,Baba E

更新日期：2009-02-01 00:00:00

abstract：:Simkania negevensis is a recently discovered chlamydia-like intracellular microorganism which has been associated with bronchiolitis in infants and with community-acquired pneumonia in adults; a high seroprevalence of antibodies to the microorganism has been found in various population groups. S. negevensis can be gro...

journal_title：Applied and environmental microbiology

authors： Kahane S,Platzner N,Dvoskin B,Itzhaki A,Friedman MG

更新日期：2004-06-01 00:00:00

abstract：:The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the...

journal_title：Applied and environmental microbiology

authors： Dehority BA,Tirabasso PA

更新日期：1989-12-01 00:00:00

abstract：:The anaerobic bacterium Eubacterium lentum, a common constituent of the intestinal microflora, inactivates digoxin by reducing the unsaturated lactone ring. Reduction of the cardiac glycoside by growing cultures of E. lentum ATCC 25559 proceeded in a stereospecific manner, with the 20R-dihydrodigoxin constituting more...

journal_title：Applied and environmental microbiology

authors： Robertson LW,Chandrasekaran A,Reuning RH,Hui J,Rawal BD

更新日期：1986-06-01 00:00:00

abstract：:The goal of this field study was to provide insight into three distinct populations of microorganisms involved in in situ metabolism of phenol. Our approach measured 13CO2 respired from [13C]phenol and stable isotope probing (SIP) of soil DNA at an agricultural field site. Traditionally, SIP-based investigations have ...

journal_title：Applied and environmental microbiology

authors： DeRito CM,Pumphrey GM,Madsen EL

更新日期：2005-12-01 00:00:00

abstract：UNLABELLED:The efficiency of direct steam injection (DSI) at 105 °C for 3 s to inactivate Mycobacterium avium subsp. paratuberculosis in milk at a pilot-plant scale was investigated. Milk samples were artificially contaminated with M. avium subsp. paratuberculosis and also with cow fecal material naturally infected wit...

journal_title：Applied and environmental microbiology

authors： Peterz M,Butot S,Jagadeesan B,Bakker D,Donaghy J

更新日期：2016-04-18 00:00:00

abstract：:Hybrubins are "unnatural" alkaloids with the same 4'-methoxy-2,2'-bipyrrole-5'-methine moiety found in prodiginines and a different ring derived from tetramic acids. Here, we demonstrated that RedH, a homologue of prodigiosin synthetase PigC, was responsible for the biosynthesis of hybrubins A and B in Streptomyces li...

journal_title：Applied and environmental microbiology

authors： Long Q,Jeffries DE,Lin S,Chen X,He W,Wang Y,Deng Z,Lindsley CW,Tao M

更新日期：2020-01-07 00:00:00

abstract：:Demineralization reduced heat resistance of B. subtilis spores, but the pattern and magnitude of the reduction depended on sporulation temperature and on heating menstruum pH. The differences in heat resistance of native spores caused by sporulation temperature almost disappeared after demineralization. Demineralized ...

journal_title：Applied and environmental microbiology

authors： Palop A,Sala FJ,Condón S

更新日期：1999-03-01 00:00:00

abstract：:During batch fermentation, the rate of ethanol production per milligram of cell protein is maximal for a brief period early in this process and declines progressively as ethanol accumulates in the surrounding broth. Our studies demonstrate that the removal of this accumulated ethanol does not immediately restore ferme...

journal_title：Applied and environmental microbiology

authors： Dombek KM,Ingram LO

更新日期：1987-06-01 00:00:00

abstract：:Presumptive bacteriophage P1 transductants of Escherichia coli, isolated from soil inoculated with lysates of transducing phage P1 and E. coli, were confirmed to be lysogenic for phage P1 by hybridization with a biotinylated DNA probe prepared from the 1.2-kilobase-pair HindIII 3 fragment of bacteriophage P1. No P1 ly...

journal_title：Applied and environmental microbiology

authors： Zeph LR,Stotzky G

更新日期：1989-03-01 00:00:00

abstract：:Current methods for detection of Cryptosporidium parvum oocysts in water are time-consuming and difficult. We have developed a reverse transcription (RT)-PCR which can detect the presence of a single viable oocyst spiked into concentrated environmental water samples. The test is based on the detection of mRNA from a C...

journal_title：Applied and environmental microbiology

authors： Stinear T,Matusan A,Hines K,Sandery M

更新日期：1996-09-01 00:00:00

abstract：:The use of two semisolid media (semisolid Rappaport and semisolid Rappaport-Vassiliadis) for the isolation of Salmonella spp. from coastal waters was compared with the use of conventional media. Two hundred and fifty six samples were studied; Salmonella spp. were detected in 83. The semisolid media were the most sensi...

journal_title：Applied and environmental microbiology

authors： Perales I,Audicana A

更新日期：1989-11-01 00:00:00

abstract：:We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...

journal_title：Applied and environmental microbiology

authors： Bulat SA,Lübeck M,Alekhina IA,Jensen DF,Knudsen IM,Lübeck PS

更新日期：2000-11-01 00:00:00

abstract：:Several wild-type isolates of marine bdellovibrios formed stable bdelloplasts when they infected gram-negative bacterial prey under certain culture conditions. Synchronous predator-prey cultures and low nutrient concentrations increased the yield of stable bdelloplasts. The bdellovibrio cells retained in the stable bd...

journal_title：Applied and environmental microbiology

authors： Sánchez-Amat A,Torrella F

更新日期：1990-09-01 00:00:00

abstract：:1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been consider...

journal_title：Applied and environmental microbiology

authors： Monincová M,Prokop Z,Vévodová J,Nagata Y,Damborsky J

更新日期：2007-03-01 00:00:00

abstract：:Arthrobacter sp. strain PBA metabolized phenylboronic acid to phenol. The oxygen atom in phenol was shown to be derived from the atmosphere using (18)O(2). 1-Naphthalene-, 2-naphthalene-, 3-cyanophenyl-, 2,5-fluorophenyl-, and 3-thiophene-boronic acids were also transformed to monooxygenated products. The oxygen atom ...

journal_title：Applied and environmental microbiology

authors： Negrete-Raymond AC,Weder B,Wackett LP

更新日期：2003-07-01 00:00:00

abstract：:Human noroviruses (HuNoVs) are the leading cause of food-borne illness, accounting for 58% of U.S. cases. Because HuNoVs are unculturable, surrogates are needed to investigate transmission routes and evaluate disinfection methods. However, the current surrogates, feline calicivirus (FCV) and murine NoV (MNV), are less...

journal_title：Applied and environmental microbiology

authors： Wang Q,Zhang Z,Saif LJ

更新日期：2012-06-01 00:00:00

abstract：:Volume 61, no. 4, p. 1211, column 2, line 40: this sentence should read as follows. "The viable-culture methods for Legionella sp. detection, however, often fail, and the Centers for Disease Control and Prevention has turned, when necessary, to PCR for epidemiological investigations of Legionnaires' disease and Pontia...

journal_title：Applied and environmental microbiology

authors： Atlas RM,Williams JF,Huntington MK

更新日期：1996-04-01 00:00:00

abstract：:Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA...

journal_title：Applied and environmental microbiology

authors： McAnulla C,Woodall CA,McDonald IR,Studer A,Vuilleumier S,Leisinger T,Murrell JC

更新日期：2001-01-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive identificat...

journal_title：Applied and environmental microbiology

authors： Valentine N,Wunschel S,Wunschel D,Petersen C,Wahl K

更新日期：2005-01-01 00:00:00

abstract：:Methylosinus trichosporium OB3b produces an extracellular copper-binding ligand (CBL) with high affinity for copper. Wild-type cells and mutants that express soluble methane monooxygenase (sMMO) in the presence and absence of copper (sMMOc) were used to obtain cell exudates that were separated and analyzed by size exc...

journal_title：Applied and environmental microbiology

authors： Téllez CM,Gaus KP,Graham DW,Arnold RG,Guzman RZ

更新日期：1998-03-01 00:00:00

abstract：:Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F(70)(10) of 1, 3, and 5 min), ...

journal_title：Applied and environmental microbiology

authors： Seyer K,Lessard M,Piette G,Lacroix M,Saucier L

更新日期：2003-06-01 00:00:00

abstract：:The effects of antibiotic production on rhizosphere microbial communities of field-grown Phaseolus vulgaris were assessed by using ribosomal intergenic spacer analysis. Inoculum strains of Rhizobium etli CE3 differing only in trifolitoxin production were used. Trifolitoxin production dramatically reduced the diversity...

journal_title：Applied and environmental microbiology

authors： Robleto EA,Borneman J,Triplett EW

更新日期：1998-12-01 00:00:00