Effects of bacterial antibiotic production on rhizosphere microbial communities from a culture-independent perspective

abstract：:Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We pr...

journal_title：Applied and environmental microbiology

authors： Hashimoto Si,Katsumata R

更新日期：1999-06-01 00:00:00

abstract：:Biofilm-associated bacteria are less sensitive to antibiotics than free-living (planktonic) cells. Furthermore, with variations in the concentration of antibiotics throughout a biofilm, microbial cells are often exposed to levels below inhibitory concentrations and may develop resistance. This, as well as the irrespon...

journal_title：Applied and environmental microbiology

authors： Algburi A,Comito N,Kashtanov D,Dicks LMT,Chikindas ML

更新日期：2017-01-17 00:00:00

abstract：:The lactose operon from Lactobacillus casei is regulated by very tight glucose repression and substrate induction mechanisms, which made it a tempting candidate system for the expression of foreign genes or metabolic engineering. An integrative vector was constructed, allowing stable gene insertion in the chromosomal ...

journal_title：Applied and environmental microbiology

authors： Gosalbes MJ,Esteban CD,Galán JL,Pérez-Martínez G

更新日期：2000-11-01 00:00:00

abstract：:Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

journal_title：Applied and environmental microbiology

authors： Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

更新日期：2006-06-01 00:00:00

abstract：:A spoilage organism isolated from turbid beer is described. The bacterium was gram negative, catalase negative, strictly anaerobic, and rod shaped, having flagella only on one side of the cell. The main metabolic product was propionic acid. In addition acetic, succinic, and lactic acids and acetoin were formed. Malona...

journal_title：Applied and environmental microbiology

authors： Haikara A,Penttilä L,Enari TM,Lounatmaa K

更新日期：1981-02-01 00:00:00

abstract：:A group-specific PCR-based denaturing gradient gel electrophoresis (DGGE) method was developed and combined with group-specific clone library analysis to investigate the diversity of the Clostridium leptum subgroup in human feces. PCR products (length, 239 bp) were amplified using C. leptum cluster-specific primers an...

journal_title：Applied and environmental microbiology

authors： Shen J,Zhang B,Wei G,Pang X,Wei H,Li M,Zhang Y,Jia W,Zhao L

更新日期：2006-08-01 00:00:00

abstract：:Rhodococcus equi is a leading cause of severe pneumonia in foals. Standard treatment is dual antimicrobial therapy with a macrolide and rifampin, but the emergence of macrolide- and rifampin-resistant R. equi isolates is an increasing problem. The objective of this study was to determine the effect of macrolide and/or...

journal_title：Applied and environmental microbiology

authors： Willingham-Lane JM,Berghaus LJ,Berghaus RD,Hart KA,Giguère S

更新日期：2019-03-22 00:00:00

abstract：:Saturation point mutagenesis was carried out at position 479 in the polyhydroxyalkanoate (PHA) synthase from Chromobacterium sp. strain USM2 (PhaC(Cs)) with specificities for short-chain-length (SCL) [(R)-3-hydroxybutyrate (3HB) and (R)-3-hydroxyvalerate (3HV)] and medium-chain-length (MCL) [(R)-3-hydroxyhexanoate (3H...

journal_title：Applied and environmental microbiology

authors： Chuah JA,Tomizawa S,Yamada M,Tsuge T,Doi Y,Sudesh K,Numata K

更新日期：2013-06-01 00:00:00

abstract：:SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated. ...

journal_title：Applied and environmental microbiology

authors： Benbadis L,Garel JR,Hartley DL

更新日期：1991-12-01 00:00:00

abstract：:Viruses are the most abundant and diverse biological entities within soils, yet their ecological impact is largely unknown. Defining how soil viral communities change with perturbation or across environments will contribute to understanding the larger ecological significance of soil viruses. A new approach to examinin...

journal_title：Applied and environmental microbiology

authors： Srinivasiah S,Lovett J,Polson S,Bhavsar J,Ghosh D,Roy K,Fuhrmann JJ,Radosevich M,Wommack KE

更新日期：2013-09-01 00:00:00

abstract：:Biodegradability screening tests of soil commonly measure 14CO2 evolution from radiolabeled test compounds, and glucose has often served as a positive control. When constant amounts of radiolabel were added to soil in combination with increasing amounts of unlabeled substrates, glucose and some related hexoses behaved...

journal_title：Applied and environmental microbiology

authors： Shen J,Bartha R

更新日期：1996-07-01 00:00:00

abstract：:Perchlorate contamination can be microbially respired to innocuous chloride and thus can be treated effectively. However, monitoring a bioremediative strategy is often difficult due to the complexities of environmental samples. Here we demonstrate that microbial respiration of perchlorate results in a significant frac...

journal_title：Applied and environmental microbiology

authors： Coleman ML,Ader M,Chaudhuri S,Coates JD

更新日期：2003-08-01 00:00:00

abstract：:pAM beta 1, a 26.5-kilobase plasmid originally isolated from Streptococcus faecalis, was conjugally transferred from Streptococcus lactis to Bacillus subtilis. No conjugal transfer of pAM beta 1 from B. subtilis to S. lactis was observed. In addition, pAM beta 1 which had been reintroduced in S. lactis after cycling t...

journal_title：Applied and environmental microbiology

authors： van der Lelie D,Venema G

更新日期：1987-10-01 00:00:00

abstract：:Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samp...

journal_title：Applied and environmental microbiology

authors： Wahlbom CF,Cordero Otero RR,van Zyl WH,Hahn-Hägerdal B,Jönsson LJ

更新日期：2003-02-01 00:00:00

abstract：:We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex geno...

journal_title：Applied and environmental microbiology

authors： Wang S,He J,Cui Z,Li S

更新日期：2007-08-01 00:00:00

abstract：:Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and phylogenetic analysis of 18S rRNA gene fragments, as well as culturing, were employed to obtain insight into the sedimen...

journal_title：Applied and environmental microbiology

authors： Brad T,Braster M,van Breukelen BM,van Straalen NM,Röling WF

更新日期：2008-07-01 00:00:00

abstract：:The extremely thermoacidophilic archaeon Metallosphaera sedula (optimum growth temperature, 73°C, pH 2.0) grows chemolithoautotrophically on metal sulfides or molecular hydrogen by employing the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) carbon fixation cycle. This cycle adds two CO2 molecules to acetyl coenzyme ...

journal_title：Applied and environmental microbiology

authors： Hawkins AB,Adams MW,Kelly RM

更新日期：2014-04-01 00:00:00

abstract：:The environmental transport of Cryptosporidium spp. through combined sewer overflow (CSO) and the occurrence of several emerging human-pathogenic Cryptosporidium species in developing countries remain unclear. In this study, we collected 40 CSO samples and 40 raw wastewater samples from Shanghai, China, and examined t...

journal_title：Applied and environmental microbiology

authors： Huang C,Hu Y,Wang L,Wang Y,Li N,Guo Y,Feng Y,Xiao L

更新日期：2017-08-01 00:00:00

abstract：:A field collected population of Plutella xylostella (SERD4) was selected in the laboratory with Bacillus thuringiensis endotoxins Cry1Ac (Cry1Ac-SEL) and Cry1Ab (Cry1Ab-SEL). Both subpopulations showed similar phenotypes: high resistance to the Cry1A toxins and little cross-resistance to Cry1Ca or Cry1D. A previous an...

journal_title：Applied and environmental microbiology

authors： Sayyed AH,Gatsi R,Ibiza-Palacios MS,Escriche B,Wright DJ,Crickmore N

更新日期：2005-11-01 00:00:00

abstract：:Laboratory strains of Saccharomyces cerevisiae have been widely used as a model for studying eukaryotic cells and mapping the molecular mechanisms of many different human diseases. Industrial wine yeasts, on the other hand, have been selected on the basis of their adaptation to stringent environmental conditions and t...

journal_title：Applied and environmental microbiology

authors： Pizarro FJ,Jewett MC,Nielsen J,Agosin E

更新日期：2008-10-01 00:00:00

abstract：:Biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. Solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. A solvent-tolerant desulfurizing bacterium, Pseudomonas putida A4, was constructed by int...

journal_title：Applied and environmental microbiology

authors： Tao F,Yu B,Xu P,Ma CQ

更新日期：2006-07-01 00:00:00

abstract：:Most denitrifiers produce nitrous oxide (N(2)O) instead of dinitrogen (N(2)) under aerobic conditions. We isolated and characterized novel aerobic denitrifiers that produce low levels of N(2)O under aerobic conditions. We monitored the denitrification activities of two of the isolates, strains TR2 and K50, in batch an...

journal_title：Applied and environmental microbiology

authors： Takaya N,Catalan-Sakairi MA,Sakaguchi Y,Kato I,Zhou Z,Shoun H

更新日期：2003-06-01 00:00:00

abstract：:We recently showed that expressing an H(2)O-NADH oxidase in Saccharomyces cerevisiae drastically reduces the intracellular NADH concentration and substantially alters the distribution of metabolic fluxes in the cell. Although the engineered strain produces a reduced amount of ethanol, a high level of acetaldehyde accu...

journal_title：Applied and environmental microbiology

authors： Heux S,Sablayrolles JM,Cachon R,Dequin S

更新日期：2006-09-01 00:00:00

abstract：:Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

journal_title：Applied and environmental microbiology

authors： Rapala J,Sivonen K,Lyra C,Niemelä SI

更新日期：1997-06-01 00:00:00

abstract：:The subsurface of a tidal-flat sediment was analyzed down to 360 cm in depth by molecular and geochemical methods. A community structure analysis of all three domains of life was performed using domain-specific PCR followed by denaturing gradient gel electrophoresis analysis and sequencing of characteristic bands. The...

journal_title：Applied and environmental microbiology

authors： Wilms R,Sass H,Köpke B,Köster J,Cypionka H,Engelen B

更新日期：2006-04-01 00:00:00

abstract：:Smectite clay minerals are abundant in soils and sediments worldwide and are typically rich in Fe. While recent investigations have shown that the structural Fe(III) bound in clay minerals is reduced by microorganisms, previous studies have not tested growth with clay minerals as the sole electron acceptor. Here we ha...

journal_title：Applied and environmental microbiology

authors： Kostka JE,Dalton DD,Skelton H,Dollhopf S,Stucki JW

更新日期：2002-12-01 00:00:00

abstract：:Clostridium perfringens food poisoning is caused mainly by enterotoxigenic type A isolates that typically possess high spore heat resistance. Previous studies have shown that alpha/beta-type small, acid-soluble proteins (SASP) play a major role in the resistance of Bacillus subtilis and C. perfringens spores to moist ...

journal_title：Applied and environmental microbiology

authors： Paredes-Sabja D,Sarker N,Setlow B,Setlow P,Sarker MR

更新日期：2008-06-01 00:00:00

abstract：:The isophthalate (IPA) degradation gene cluster (iphACBDR) responsible for the conversion of IPA into protocatechuate (PCA) was isolated from Comamonas sp. strain E6, which utilizes phthalate isomers as sole carbon and energy sources via the PCA 4,5-cleavage pathway. Based on amino acid sequence similarity, the iphA, ...

journal_title：Applied and environmental microbiology

authors： Fukuhara Y,Inakazu K,Kodama N,Kamimura N,Kasai D,Katayama Y,Fukuda M,Masai E

更新日期：2010-01-01 00:00:00

abstract：:A number of peptides were evaluated as chemoattractants for Pseudomonas aeruginosa. Several strains recognized tri-, tetra-, penta-, and hexapeptides in a capillary tube assay. Tripeptides altered at the carboxyl terminus were good attractants, whereas tripeptides altered at the amino terminus did not serve as chemoat...

journal_title：Applied and environmental microbiology

authors： Kelly-Wintenberg K,Montie TC

更新日期：1994-01-01 00:00:00

abstract：:For decades, untreated sewage flowing northward from Tijuana, Mexico, via the Tijuana River has adversely affected the water quality of the recreational beaches of San Diego, California. We used quantitative reverse transcription-PCR to measure the levels of hepatitis A virus (HAV) and enteroviruses in coastal waters ...

journal_title：Applied and environmental microbiology

authors： Gersberg RM,Rose MA,Robles-Sikisaka R,Dhar AK

更新日期：2006-12-01 00:00:00