Aerobic denitrifying bacteria that produce low levels of nitrous oxide.


:Most denitrifiers produce nitrous oxide (N(2)O) instead of dinitrogen (N(2)) under aerobic conditions. We isolated and characterized novel aerobic denitrifiers that produce low levels of N(2)O under aerobic conditions. We monitored the denitrification activities of two of the isolates, strains TR2 and K50, in batch and continuous cultures. Both strains reduced nitrate (NO(3)(-)) to N(2) at rates of 0.9 and 0.03 micro mol min(-1) unit of optical density at 540 nm(-1) at dissolved oxygen (O(2)) (DO) concentrations of 39 and 38 micro mol liter(-1), respectively. At the same DO level, the typical denitrifier Pseudomonas stutzeri and the previously described aerobic denitrifier Paracoccus denitrificans did not produce N(2) but evolved more than 10-fold more N(2)O than strains TR2 and K50 evolved. The isolates denitrified NO(3)(-) with concomitant consumption of O(2). These results indicated that strains TR2 and K50 are aerobic denitrifiers. These two isolates were taxonomically placed in the beta subclass of the class Proteobacteria and were identified as P. stutzeri TR2 and Pseudomonas sp. strain K50. These strains should be useful for future investigations of the mechanisms of denitrifying bacteria that regulate N(2)O emission, the single-stage process for nitrogen removal, and microbial N(2)O emission into the ecosystem.


Appl Environ Microbiol


Takaya N,Catalan-Sakairi MA,Sakaguchi Y,Kato I,Zhou Z,Shoun H





Has Abstract


2003-06-01 00:00:00












  • Diverse and unique picocyanobacteria in Chesapeake Bay, revealed by 16S-23S rRNA internal transcribed spacer sequences.

    abstract::rRNA internal transcribed spacer phylogeny showed that Chesapeake Bay is populated with diverse Synechococcus strains, including members of the poorly studied marine cluster B. Marine cluster B prevailed in the upper bay, while marine cluster A was common in the lower bay. Interestingly, marine cluster B Synechococcus...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Chen F,Wang K,Kan J,Suzuki MT,Wommack KE

    更新日期:2006-03-01 00:00:00

  • Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides.

    abstract::Aryl-alcohol oxidase (AAO) is an extracellular flavoprotein that supplies ligninolytic peroxidases with H2O2 during natural wood decay. With a broad substrate specificity and highly stereoselective reaction mechanism, AAO is an attractive candidate for studies into organic synthesis and synthetic biology, and yet the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Viña-Gonzalez J,Gonzalez-Perez D,Ferreira P,Martinez AT,Alcalde M

    更新日期:2015-09-01 00:00:00

  • Development and characterization of lactose-positive pediococcus species for milk fermentation.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Caldwell SL,McMahon DJ,Oberg CJ,Broadbent JR

    更新日期:1996-03-01 00:00:00

  • Microbial community composition and denitrifying enzyme activities in salt marsh sediments.

    abstract::Denitrifying microbial communities and denitrification in salt marsh sediments may be affected by many factors, including environmental conditions, nutrient availability, and levels of pollutants. The objective of this study was to examine how microbial community composition and denitrification enzyme activities (DEA)...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Cao Y,Green PG,Holden PA

    更新日期:2008-12-01 00:00:00

  • Mosquitocidal activity of the CryIC delta-endotoxin from Bacillus thuringiensis subsp. aizawai.

    abstract::The cloned 135-kDa CryIC delta-endotoxin from Bacillus thuringiensis is a lepidopteran-active toxin, displaying high activity in vivo against Spodoptera litoralis and Spodoptera frugiperda larvae and in vitro against the S. frugiperda Sf9 cell line. Here, we report that the CryIC delta-endotoxin cloned from B. thuring...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Smith GP,Merrick JD,Bone EJ,Ellar DJ

    更新日期:1996-02-01 00:00:00

  • Identification of disulfides from the biodegradation of dibenzothiophene.

    abstract::Several investigations have identified benzothiophene-2,3-dione in the organic solvent extracts of acidified cultures degrading dibenzothiophene via the Kodama pathway. In solution at neutral pH, the 2,3-dione exists as 2-mercaptophenylglyoxylate, which cyclizes upon acidification and is extracted as the 2,3-dione. Th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bressler DC,Fedorak PM

    更新日期:2001-11-01 00:00:00

  • Effects of High Pressure on Inactivation Kinetics and Events Related to Proton Efflux in Lactobacillus plantarum.

    abstract::Knowledge of the mechanism of pressure-induced inactivation of microorganisms could be helpful in defining an effective, relatively mild pressure treatment as a means of decontamination, especially in combination with other physical treatments or antimicrobial agents. We have studied the effect of high pressure on Lac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wouters PC,Glaasker E,Smelt JP

    更新日期:1998-02-01 00:00:00

  • Nitrate-dependent ferrous iron oxidation by anaerobic ammonium oxidation (anammox) bacteria.

    abstract::We examined nitrate-dependent Fe(2+) oxidation mediated by anaerobic ammonium oxidation (anammox) bacteria. Enrichment cultures of "Candidatus Brocadia sinica" anaerobically oxidized Fe(2+) and reduced NO3(-) to nitrogen gas at rates of 3.7 ± 0.2 and 1.3 ± 0.1 (mean ± standard deviation [SD]) nmol mg protein(-1) min(-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Oshiki M,Ishii S,Yoshida K,Fujii N,Ishiguro M,Satoh H,Okabe S

    更新日期:2013-07-01 00:00:00

  • Moderate prevalence of antimicrobial resistance in Escherichia coli isolates from lettuce, irrigation water, and soil.

    abstract::Fresh produce is known to carry nonpathogenic epiphytic microorganisms. During agricultural production and harvesting, leafy greens can become contaminated with antibiotic-resistant pathogens or commensals from animal and human sources. As lettuce does not undergo any inactivation or preservation treatment during proc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Holvoet K,Sampers I,Callens B,Dewulf J,Uyttendaele M

    更新日期:2013-11-01 00:00:00

  • Toolbox Approaches Using Molecular Markers and 16S rRNA Gene Amplicon Data Sets for Identification of Fecal Pollution in Surface Water.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ahmed W,Staley C,Sadowsky MJ,Gyawali P,Sidhu JP,Palmer A,Beale DJ,Toze S

    更新日期:2015-10-01 00:00:00

  • Viability of Rhizobium bacteroids.

    abstract::Bacteroids prepared from nodules of soybean and bean were tested for viability. Contrary to the prevailing view that bacteroids are nonviable, it was found that bacteroids averaged 90% viability, irrespective of Rhizobium strain, nodule age, or nodule environment. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Tsien HC,Cain PS,Schmidt EL

    更新日期:1977-12-01 00:00:00

  • Penicillic acid production in submerged culture.

    abstract::Twenty known penicillic acid (PA)-producing Aspergillus and Penicillium cultures were grown under various conditions in shaken flasks to determine the highest yielding strains and their requirements for maximum toxin production. Abilities of the cultures to utilize eight different carbon sources in Raulin-Thom medium ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Lindenfelser LA,Ciegler A

    更新日期:1977-11-01 00:00:00

  • Interactions of Salmonella enterica Serovar Typhimurium and Pectobacterium carotovorum within a Tomato Soft Rot.

    abstract::Salmonella spp. are remarkably adaptable pathogens, and this adaptability allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amid the native microbiota remain poorly understood. Here, we aimed to uncover the mechanisms that enable ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: George AS,Cox CE,Desai P,Porwollik S,Chu W,de Moraes MH,McClelland M,Brandl MT,Teplitski M

    更新日期:2018-02-14 00:00:00

  • Phylogenetic analysis and in situ identification of bacteria community composition in an acidic Sphagnum peat bog.

    abstract::The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was clos...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Dedysh SN,Pankratov TA,Belova SE,Kulichevskaya IS,Liesack W

    更新日期:2006-03-01 00:00:00

  • Influence of the membrane on T-2 toxin toxicity in Saccharomyces spp.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Schappert KT,Khachatourians GG

    更新日期:1984-04-01 00:00:00

  • Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence.

    abstract::Entomopathogenic fungi can produce a series of chitinases, some of which act synergistically with proteases to degrade insect cuticle. However, chitinase involvement in insect fungus pathogenesis has not been fully characterized. In this paper, an endochitinase, Bbchit1, was purified to homogeneity from liquid culture...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Fang W,Leng B,Xiao Y,Jin K,Ma J,Fan Y,Feng J,Yang X,Zhang Y,Pei Y

    更新日期:2005-01-01 00:00:00

  • Role of fadR and atoC(Con) mutations in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in recombinant pha+ Escherichia coli.

    abstract::Recombinant Escherichia coli fadR atoC(Con) mutants containing the polyhydroxyalkanoate (PHA) biosynthesis genes from Alcaligenes eutrophus are able to incorporate significant levels of 3-hydroxyvalerate (3HV) into the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)]. We have used E. coli fadR (F...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Rhie HG,Dennis D

    更新日期:1995-07-01 00:00:00

  • Gray mold populations in german strawberry fields are resistant to multiple fungicides and dominated by a novel clade closely related to Botrytis cinerea.

    abstract::The gray mold fungus Botrytis cinerea is a major threat to fruit and vegetable production. Strawberry fields usually receive several fungicide treatments against Botrytis per season. Gray mold isolates from several German strawberry-growing regions were analyzed to determine their sensitivity against botryticides. Fun...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Leroch M,Plesken C,Weber RW,Kauff F,Scalliet G,Hahn M

    更新日期:2013-01-01 00:00:00

  • Phylogeny of acetate-utilizing microorganisms in soils along a nutrient gradient in the Florida Everglades.

    abstract::The consumption of acetate in soils taken from a nutrient gradient in the northern Florida Everglades was studied by stable isotope probing. Bacterial and archaeal 16S rRNA gene clone libraries from eutrophic and oligotrophic soil microcosms strongly suggest that a significant amount of acetate is consumed by syntroph...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Chauhan A,Ogram A

    更新日期:2006-10-01 00:00:00

  • Potential role of Diploscapter sp. strain LKC25, a bacterivorous nematode from soil, as a vector of food-borne pathogenic bacteria to preharvest fruits and vegetables.

    abstract::Diploscapter, a thermotolerant, free-living soil bacterial-feeding nematode commonly found in compost, sewage, and agricultural soil in the United States, was studied to determine its potential role as a vehicle of Salmonella enterica serotype Poona, enterohemorrhagic Escherichia coli O157:H7, and Listeria monocytogen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Gibbs DS,Anderson GL,Beuchat LR,Carta LK,Williams PL

    更新日期:2005-05-01 00:00:00

  • Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain.

    abstract::Stepwise deletions in the only plasmid in Thermus thermophilus HB27, megaplasmid pTT27, showed that two distantly located loci were important for maintenance of the plasmid. One is a minimum replicon including one gene, repT, coding a replication initiator, and the other encodes subunits of class I ribonucleotide redu...

    journal_title:Applied and environmental microbiology

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    authors: Ohtani N,Tomita M,Itaya M

    更新日期:2015-12-28 00:00:00

  • Heat-stable proteases from psychrotrophic pseudomonads: comparison of immunological properties.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Jackman DM,Bartlett FM,Patel TR

    更新日期:1983-07-01 00:00:00

  • Novel Modifications of Nonribosomal Peptides from Brevibacillus laterosporus MG64 and Investigation of Their Mode of Action.

    abstract::Nonribosomal peptides (NRPs) are a class of secondary metabolites usually produced by microorganisms. They are of paramount importance in different applications, including biocontrol and pharmacy. Brevibacillus spp. are a rich source of NRPs yet have received little attention. In this study, we characterize four novel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Li Z,de Vries RH,Chakraborty P,Song C,Zhao X,Scheffers DJ,Roelfes G,Kuipers OP

    更新日期:2020-11-24 00:00:00

  • Gene identification and substrate regulation provide insights into sulfur accumulation during bioleaching with the psychrotolerant acidophile Acidithiobacillus ferrivorans.

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    pub_type: 杂志文章


    authors: Liljeqvist M,Rzhepishevska OI,Dopson M

    更新日期:2013-02-01 00:00:00

  • Comparative analysis of antimicrobial activities of valinomycin and cereulide, the Bacillus cereus emetic toxin.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Tempelaars MH,Rodrigues S,Abee T

    更新日期:2011-04-01 00:00:00

  • Important Metabolic Pathways and Biological Processes Expressed by Chicken Cecal Microbiota.

    abstract::The gut microbiota plays important roles in its host. However, how each microbiota member contributes to the behavior of the whole population is not known. In this study, we therefore determined protein expression in the cecal microbiota in chickens of selected ages and in 7-day-old chickens inoculated with different ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Polansky O,Sekelova Z,Faldynova M,Sebkova A,Sisak F,Rychlik I

    更新日期:2015-12-28 00:00:00

  • Serotyping, stx2 subtyping, and characterization of the locus of enterocyte effacement island of shiga toxin-producing Escherichia coli and E. coli O157:H7 strains isolated from the environment in France.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Vernozy-Rozand C,Montet MP,Bertin Y,Trably F,Girardeau JP,Martin C,Livrelli V,Beutin L

    更新日期:2004-04-01 00:00:00

  • Influence of environmental factors and human activity on the presence of Salmonella serovars in a marine environment.

    abstract::The temporal and spatial distribution of Salmonella contamination in the coastal waters of Galicia (northwestern Spain) relative to contamination events with different environmental factors (temperature, wind, hours of sunlight, rainfall, and river flow) were investigated over a 4-year period. Salmonellae were isolate...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Martinez-Urtaza J,Saco M,de Novoa J,Perez-Piñeiro P,Peiteado J,Lozano-Leon A,Garcia-Martin O

    更新日期:2004-04-01 00:00:00

  • Methanogenic transformation of methylfurfural compounds to furfural.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Boopathy R

    更新日期:1996-09-01 00:00:00

  • Survival of Azospirillum brasilense in the Bulk Soil and Rhizosphere of 23 Soil Types.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bashan Y,Puente ME,Rodriguez-Mendoza MN,Toledo G,Holguin G,Ferrera-Cerrato R,Pedrin S

    更新日期:1995-05-01 00:00:00