Comparative analysis of eukaryotic marine microbial assemblages from 18S rRNA gene and gene transcript clone libraries by using different methods of extraction.

abstract：:The diversity of fungi in permanently frozen soil from northeastern Siberia was studied by culture-independent PCR amplification of diverse environmental 18S rRNA genes. Elaborate protocols to avoid contamination during drilling, sampling, and amplification were used. A broad diversity of eukaryotic DNA sequences that...

journal_title：Applied and environmental microbiology

authors： Lydolph MC,Jacobsen J,Arctander P,Gilbert MT,Gilichinsky DA,Hansen AJ,Willerslev E,Lange L

更新日期：2005-02-01 00:00:00

abstract：:The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid medium (composed of ...

journal_title：Applied and environmental microbiology

authors： Sakai DK

更新日期：1985-10-01 00:00:00

abstract：:The fate of 14C-labeled 2,4,6-trinitrotoluene (TNT) in an activated-sludge system was investigated. No [14C]TNT could be detected in the contents of an aerated reactor after 3 to 5 days of incubation. No significant 14CO2 was formed, and the radioactivity was about equally divided between the floc and the supernatant....

journal_title：Applied and environmental microbiology

authors： Carpenter DF,McCormick NG,Cornell JH,Kaplan AM

更新日期：1978-05-01 00:00:00

abstract：:We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture. ...

journal_title：Applied and environmental microbiology

authors： Hirata Y,Minami J,Koyama M,Matsushita O,Katayama S,Jin F,Maeta H,Okabe A

更新日期：1995-11-01 00:00:00

abstract：:A study was conducted to determine the influence of arbuscular mycorrhizal (AM) fungi on Salmonella and enterohemorrhagic Escherichia coli O157:H7 (EHEC) in autoclaved soil and translocation into leek plants. Six-week-old leek plants (with [Myc+] or without [Myc-] AM fungi) were inoculated with composite suspensions o...

journal_title：Applied and environmental microbiology

authors： Gurtler JB,Douds DD Jr,Dirks BP,Quinlan JJ,Nicholson AM,Phillips JG,Niemira BA

更新日期：2013-03-01 00:00:00

abstract：:Anaerobic degradation of monochlorophenols and monochlorobenzoates in a variety of aquatic sediments was compared under four enrichment conditions. A broader range of compounds was degraded in enrichments inoculated with sediment exposed to industrial effluents. Degradation of chloroaromatic compounds was observed mos...

journal_title：Applied and environmental microbiology

authors： Genthner BR,Price WA,Pritchard PH

更新日期：1989-06-01 00:00:00

abstract：:A UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) d- and l-sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucose sugar-conjugated resveratrol glycosides. Significantly higher (90%) bioconversion of resverat...

journal_title：Applied and environmental microbiology

authors： Pandey RP,Parajuli P,Shin JY,Lee J,Lee S,Hong YS,Park YI,Kim JS,Sohng JK

更新日期：2014-12-01 00:00:00

abstract：:Escherichia coli JM101(pSPZ3), containing xylene monooxygenase (XMO) from Pseudomonas putida mt-2, catalyzes specific oxidations and reductions of m-nitrotoluene and derivatives thereof. In addition to reactions catalyzed by XMO, we focused on biotransformations by native enzymes of the E. coli host and their effect o...

journal_title：Applied and environmental microbiology

authors： Meyer D,Witholt B,Schmid A

更新日期：2005-11-01 00:00:00

abstract：:Beef carcass sponge samples collected from July to August 1999 at four large processing plants in the United States were surveyed for the presence of non-O157 Shiga toxin-producing Escherichia coli (STEC). Twenty-eight (93%) of 30 single-source lots surveyed included at least one sample containing non-O157 STEC. Of 33...

journal_title：Applied and environmental microbiology

authors： Arthur TM,Barkocy-Gallagher GA,Rivera-Betancourt M,Koohmaraie M

更新日期：2002-10-01 00:00:00

abstract：:Q fever, caused by Coxiella burnetii, is a zoonosis with a worldwide distribution. A large rural area in the southeast of the Netherlands was heavily affected by Q fever between 2007 and 2009. This initiated the development of a robust and internally controlled multiplex quantitative PCR (qPCR) assay for the detection...

journal_title：Applied and environmental microbiology

authors： de Bruin A,de Groot A,de Heer L,Bok J,Wielinga PR,Hamans M,van Rotterdam BJ,Janse I

更新日期：2011-09-01 00:00:00

abstract：:Gibberellins (GAs) are tetracyclic diterpenoid phytohormones that were first identified as secondary metabolites of the fungus Fusarium fujikuroi (teleomorph, Gibberella fujikuroi). GAs were also found in the cassava pathogen Sphaceloma manihoticola, but the spectrum of GAs differed from that in F. fujikuroi. In contr...

journal_title：Applied and environmental microbiology

authors： Bömke C,Rojas MC,Gong F,Hedden P,Tudzynski B

更新日期：2008-09-01 00:00:00

abstract：:In earlier studies, the pathway of toluene and m- and p-xylene degradation (TOL pathway) in Pseudomonas putida (pWW0) was found to be subject to catabolite repression when the strain was grown at the maximal rate on glucose or succinate in the presence of an inducer. This report describes catabolite repression of the ...

journal_title：Applied and environmental microbiology

authors： Duetz WA,Marqués S,Wind B,Ramos JL,van Andel JG

更新日期：1996-02-01 00:00:00

abstract：:Transformed root cultures of three nonmycotrophic and one mycotrophic plant species stimulated germination and hyphal growth of the vesicular-arbuscular mycorrhizal fungus Glomus etunicatum (Becker & Gerd.) in a gel medium. However, only roots of the mycotrophic species (carrot) supported continued hyphal exploration ...

journal_title：Applied and environmental microbiology

authors： Schreiner RP,Koide RT

更新日期：1993-08-01 00:00:00

abstract：:Xenorhabdus nematophila, a bacterium pathogenic for insects associated with the nematode Steinernema carpocapsae, releases high quantities of proteases, which may participate in the virulence against insects. Zymogram assays and cross-reactions of antibodies suggested that two distinct proteases were present. The majo...

journal_title：Applied and environmental microbiology

authors： Caldas C,Cherqui A,Pereira A,Simões N

更新日期：2002-03-01 00:00:00

abstract：:Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola....

journal_title：Applied and environmental microbiology

authors： Bian J,Fenno JC,Li C

更新日期：2012-03-01 00:00:00

abstract：:Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of al...

journal_title：Applied and environmental microbiology

authors： Bonnarme P,Psoni L,Spinnler HE

更新日期：2000-12-01 00:00:00

abstract：:A physiological, unbalanced model is presented that explicitly describes growth of the marine cyanobacterium Trichodesmium sp. at the expense of N(2) (diazotrophy). The model involves the dynamics of intracellular reserves of carbon and nitrogen and allows the uncoupling of the metabolism of these elements. The result...

journal_title：Applied and environmental microbiology

authors： Rabouille S,Staal M,Stal LJ,Soetaert K

更新日期：2006-05-01 00:00:00

abstract：:We report the isolation and characterization of a phototrophic ferrous iron [Fe(II)]-oxidizing bacterium named TIE-1 that differs from other Fe(II)-oxidizing phototrophs in that it is genetically tractable. Under anaerobic conditions, TIE-1 grows photoautotrophically with Fe(II), H2, or thiosulfate as the electron don...

journal_title：Applied and environmental microbiology

authors： Jiao Y,Kappler A,Croal LR,Newman DK

更新日期：2005-08-01 00:00:00

abstract：:Antialgal allelochemicals were isolated from Phragmites communis Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of Chlorella pyrenoidosa and Microcystis aeruginosa but had no inhibition on Chlorella vulgaris. The 50% effective concentrations (EC50) of the allelopathic fraction...

journal_title：Applied and environmental microbiology

authors： Li FM,Hu HY

更新日期：2005-11-01 00:00:00

abstract：:Rolling circle amplification (RCA) generates large single-stranded and tandem repeats of target DNA as amplicons. This technique was applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coli cells carrying a specific gene sequence. The method features (i) one short targ...

journal_title：Applied and environmental microbiology

authors： Maruyama F,Kenzaka T,Yamaguchi N,Tani K,Nasu M

更新日期：2005-12-01 00:00:00

abstract：:Our goal was to develop a field soil biodegradation assay using (13)C-labeled compounds and identify the active microorganisms by analyzing 16S rRNA genes in soil-derived (13)C-labeled DNA. Our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soi...

journal_title：Applied and environmental microbiology

authors： Padmanabhan P,Padmanabhan S,DeRito C,Gray A,Gannon D,Snape JR,Tsai CS,Park W,Jeon C,Madsen EL

更新日期：2003-03-01 00:00:00

abstract：:The probability of recovering pathogenic Escherichia coli from food by the Bacteriological Analytical Manual method was determined by the effects of several factors: the number of strains per food, the ability of pathogenic strains to survive enrichment, and the frequency of plasmid loss during enrichment. Biochemical...

journal_title：Applied and environmental microbiology

authors： Hill WE,Ferreira JL,Payne WL,Jones VM

更新日期：1985-06-01 00:00:00

abstract：:Phylogenetic relationships, diversity, and in situ identification of spirochetes in the gut of the termite Neotermes koshunensis were examined without cultivation, with an emphasis on ectosymbionts attached to flagellated protists. Spirochetes in the gut microbial community investigated so far are related to the genus...

journal_title：Applied and environmental microbiology

authors： Noda S,Ohkuma M,Yamada A,Hongoh Y,Kudo T

更新日期：2003-01-01 00:00:00

abstract：:We detected and identified genotypes of human-pathogenic microsporidia in fecal samples from 51 asymptomatic captive-bred pet parrots in South Korea. Microsporidia were identified in 8 samples (15.7%); 7 parrots tested positive for Encephalitozoon hellem, and 1 parrot tested positive for both E. hellem and Encephalito...

journal_title：Applied and environmental microbiology

authors： Lee SY,Lee SS,Lyoo YS,Park HM

更新日期：2011-12-01 00:00:00

abstract：:The alphaproteobacterium Hyphomonas neptunium proliferates by a unique budding mechanism in which daughter cells emerge from the end of a stalk-like extension emanating from the mother cell body. Studies of this species so far have been hampered by the lack of a genetic system and of molecular tools allowing the regul...

journal_title：Applied and environmental microbiology

authors： Jung A,Eisheuer S,Cserti E,Leicht O,Strobel W,Möll A,Schlimpert S,Kühn J,Thanbichler M

更新日期：2015-01-01 00:00:00

abstract：:The genetic diversity of 35 Bacillus sphaericus strains was analyzed by a newly developed multilocus sequence typing (MLST) scheme, toxin gene pool survey, and mosquito bioassay. The results demonstrated that strains assigned to the same sequence type (ST) had the same occurrence of toxin genes. Further sequence analy...

journal_title：Applied and environmental microbiology

authors： Ge Y,Hu X,Zheng D,Wu Y,Yuan Z

更新日期：2011-08-01 00:00:00

abstract：:A method was developed to follow bacterial nitrate reduction in freshwater sediments by using common high-performance liquid chromatographic equipment. The low detection limit (14 pmol) of the method enabled us to study concentration profiles and reaction kinetics under natural conditions. Significant nitrate concentr...

journal_title：Applied and environmental microbiology

authors： Hordijk CA,Snieder M,van Engelen JJ,Cappenberg TE

更新日期：1987-02-01 00:00:00

abstract：:There is a growing interest in the use of microbial fermentation for the generation of high-demand, high-purity chemicals using cheap feedstocks in an environmentally friendly manner. One example explored here is the production of isoprene (C5H8), a hemiterpene, which is primarily polymerized to polyisoprene in synthe...

journal_title：Applied and environmental microbiology

authors： Diner BA,Fan J,Scotcher MC,Wells DH,Whited GM

更新日期：2017-12-15 00:00:00

abstract：:In Pseudomonas sp. strain M114, the outer membrane receptor for ferric pseudobactin M114 was shown to transport ferric pseudobactins B10 and A225, in addition to its own. The gene encoding this receptor, which was previously cloned on pCUP3, was localized by Tn5 mutagenesis to a region comprising >1.6 kb of M114 DNA. ...

journal_title：Applied and environmental microbiology

authors： Morris J,O'sullivan DJ,Koster M,Leong J,Weisbeek PJ,O'gara F

更新日期：1992-02-01 00:00:00

abstract：:We investigated the genetic diversity of symbiotic bacteria associated with two newly discovered species of Osedax from Monterey Canyon, CA, at 1,017-m (Osedax Monterey Bay sp. 3 "rosy" [Osedax sp. MB3]) and 381-m (Osedax Monterey Bay sp. 4 "yellow collar") depths. Quantitative PCR and clone libraries of 16S rRNA gene...

journal_title：Applied and environmental microbiology

authors： Goffredi SK,Johnson SB,Vrijenhoek RC

更新日期：2007-04-01 00:00:00