Microbial transformation of 14C-labeled 2,4,6-trinitrotoluene in an activated-sludge system.

Abstract:

:The fate of 14C-labeled 2,4,6-trinitrotoluene (TNT) in an activated-sludge system was investigated. No [14C]TNT could be detected in the contents of an aerated reactor after 3 to 5 days of incubation. No significant 14CO2 was formed, and the radioactivity was about equally divided between the floc and the supernatant. The radioactive carbon present in the microflora was mainly associated with the lipid and protein components, but the characteristic constituents of these compounds (e.g., fatty acids and amino acids) were not radioactive. The major part of the 14C present in the lipid and protein fractions was found in precipitates that formed in both fractions. The solubility properties and infrared spectra of these precipitates suggested that they are macromolecular structures of the polyamide type formed by the reaction of TNT biotransformation products with lipids, fatty acids, and protein constituents of the microbial flora. This hypothesis is further supported by the correspondence of the infrared spectrum of the lipid precipitate with that of a model compound synthesized from TNT transformation products and lipid precursors. The resistance of these macromolecules to further biodegradation was paralleled by the reported resistance to microbial attack of polyamides containing similar linkages.

journal_name

Appl Environ Microbiol

authors

Carpenter DF,McCormick NG,Cornell JH,Kaplan AM

doi

10.1128/AEM.35.5.949-954.1978

subject

Has Abstract

pub_date

1978-05-01 00:00:00

pages

949-54

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

35

pub_type

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