Cloning and nucleotide sequencing of a Staphylococcus aureus gene encoding a branched-chain-amino-acid transporter.

abstract：:This study was initiated to determine if reduced sulfur contained in coal gasifier ash and slag was available for microbial and chemical oxidation because eventual large-quantity landfill disposal of these solid wastes is expected. Continuous application of distilled water to a column containing a high-sulfur-content ...

journal_title：Applied and environmental microbiology

authors： Strayer RF,Davis EC

更新日期：1983-03-01 00:00:00

abstract：:The ammonia-oxidizing prokaryote (AOP) community in three groundwater treatment plants and connected distribution systems was analyzed by quantitative real-time PCR and sequence analysis targeting the amoA gene of ammonia-oxidizing bacteria (AOB) and archaea (AOA). Results demonstrated that AOB and AOA numbers increas...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Voost S,van der Kooij D

更新日期：2009-07-01 00:00:00

abstract：:A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon in...

journal_title：Applied and environmental microbiology

authors： Bouhenni R,Gehrke A,Saffarini D

更新日期：2005-08-01 00:00:00

abstract：:Perchlorate contamination can be microbially respired to innocuous chloride and thus can be treated effectively. However, monitoring a bioremediative strategy is often difficult due to the complexities of environmental samples. Here we demonstrate that microbial respiration of perchlorate results in a significant frac...

journal_title：Applied and environmental microbiology

authors： Coleman ML,Ader M,Chaudhuri S,Coates JD

更新日期：2003-08-01 00:00:00

abstract：:With the submersible JAGO and by scuba diving we discovered three remarkable geothermal cones, rising 33, 25, and 45 m from the seafloor at a depth of 65 m in Eyjafjordur, northern Iceland. The greatest geothermal activity was on the highest cone, which discharged up to 50 liters of freshwater per s at 72 degrees C an...

journal_title：Applied and environmental microbiology

authors： Marteinsson VT,Kristjánsson JK,Kristmannsdóttir H,Dahlkvist M,Saemundsson K,Hannington M,Pétursdóttir SK,Geptner A,Stoffers P

更新日期：2001-02-01 00:00:00

abstract：:Lysostaphin (Lst) is a potent bacteriolytic enzyme that kills Staphylococcus aureus, a common bacterial pathogen of humans and animals. With high activity against both planktonic cells and biofilms, Lst has the potential to be used in industrial products, such as commercial cleansers, for decontamination. However, Lst...

journal_title：Applied and environmental microbiology

authors： Wu X,Kwon SJ,Kim D,Zha J,Mora-Pale M,Dordick JS

更新日期：2018-07-02 00:00:00

abstract：:Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion of glycerol to ethanol. By using nine gene knockout mutations, the functional space of the central metabolism of E. coli was reduced from over 15,000 possible pathways to a total of 28 glycerol-utilizing pathways that s...

journal_title：Applied and environmental microbiology

authors： Trinh CT,Srienc F

更新日期：2009-11-01 00:00:00

abstract：:Motile phototrophic consortia are highly regular associations in which numerous cells of green sulfur bacteria surround a flagellated colorless beta-proteobacterium in the center. To date, seven different morphological types of such consortia have been described. In addition, two immotile associations involving green ...

journal_title：Applied and environmental microbiology

authors： Glaeser J,Overmann J

更新日期：2004-08-01 00:00:00

abstract：:The fate of Listeria monocytogenes during refrigerated storage was determined on several processed meat products, including ham, bologna, wieners, sliced chicken, sliced turkey, fermented semidried sausage, bratwurst, and cooked roast beef. The meats were surface inoculated with a five-strain mixture of less than or e...

journal_title：Applied and environmental microbiology

authors： Glass KA,Doyle MP

更新日期：1989-06-01 00:00:00

abstract：:The contribution of the biochemical pathways nitrification, denitrification, and dissimilatory NO3- reduction to NH4+ (DNRA) to the accumulation of NO2- in freshwaters is governed by the species compositions of the bacterial populations resident in the sediments, available carbon (C) and nitrogen (N) substrates, and e...

journal_title：Applied and environmental microbiology

authors： Kelso BHL,Smith RV,Laughlin RJ

更新日期：1999-01-01 00:00:00

abstract：:Cells of the gram-negative bacterium Ralstonia sp. strain SBUG 290 grown in the presence of biphenyl are able to cooxidize dibenzofuran which has been 1,2-hydroxylated. Meta cleavage of the 1, 2-dihydroxydibenzofuran between carbon atoms 1 and 9b produced 2-hydroxy-4-(3'-oxo-3'H-benzofuran-2'-yliden)but-2-enoic acid, ...

journal_title：Applied and environmental microbiology

authors： Becher D,Specht M,Hammer E,Francke W,Schauer F

更新日期：2000-10-01 00:00:00

abstract：:Sclerotinia sclerotiorum acidifies its ambient environment by producing oxalic acid. This production of oxalic acid during plant infection has been implicated as a primary determinant of pathogenicity in this and other phytopathogenic fungi. We found that ambient pH conditions affect multiple processes in S. sclerotio...

journal_title：Applied and environmental microbiology

authors： Rollins JA,Dickman MB

更新日期：2001-01-01 00:00:00

abstract：:The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat. ...

journal_title：Applied and environmental microbiology

authors： Makino S,Okada Y,Maruyama T

更新日期：1995-10-01 00:00:00

abstract：:Of the toxins produced by Bacillus cereus, the emetic toxin is likely the most dangerous but, due to the lack of a suitable assay, the least well known. In this paper, a new, sensitive, inexpensive, and rapid bioassay for detection of the emetic toxin of B. cereus is described. The assay is based on the loss of motili...

journal_title：Applied and environmental microbiology

authors： Andersson MA,Mikkola R,Helin J,Andersson MC,Salkinoja-Salonen M

更新日期：1998-04-01 00:00:00

abstract：:PCR-based methods have been developed to rapidly screen for Legionella pneumophila in water as an alternative to time-consuming culture techniques. However, these methods fail to discriminate between live and dead bacteria. Here, we report a viability assay (viability PCR [v-PCR]) for L. pneumophila that combines ethi...

journal_title：Applied and environmental microbiology

authors： Delgado-Viscogliosi P,Solignac L,Delattre JM

更新日期：2009-06-01 00:00:00

abstract：:A purified lipase from the yeast Cryptococcus sp. strain S-2 exhibited remote homology to proteins belonging to the cutinase family rather than to lipases. This enzyme could effectively degrade the high-molecular-weight compound polylactic acid, as well as other biodegradable plastics, including polybutylene succinate...

journal_title：Applied and environmental microbiology

authors： Masaki K,Kamini NR,Ikeda H,Iefuji H

更新日期：2005-11-01 00:00:00

abstract：:A basal (BC) medium devoid of added carbohydrates, a complete (CC) medium containing nine carbohydrates were developed for enumerating rumen bacteria. The colony counts on the BC medium were 85 to 100% of those obtained on the CC medium. These colonies were pinpoint size (less than or equal to mm in diameter) but incr...

journal_title：Applied and environmental microbiology

authors： Leedle JA,Hespell RB

更新日期：1980-04-01 00:00:00

abstract：:Twenty known penicillic acid (PA)-producing Aspergillus and Penicillium cultures were grown under various conditions in shaken flasks to determine the highest yielding strains and their requirements for maximum toxin production. Abilities of the cultures to utilize eight different carbon sources in Raulin-Thom medium ...

journal_title：Applied and environmental microbiology

authors： Lindenfelser LA,Ciegler A

更新日期：1977-11-01 00:00:00

abstract：:The alphaproteobacterium Magnetospirillum gryphiswaldense has the intriguing ability to navigate within magnetic fields, a behavior named magnetotaxis, governed by the formation of magnetosomes, intracellular membrane-enveloped crystals of magnetite. Magnetosomes are aligned in chains along the cell's motility axis by...

journal_title：Applied and environmental microbiology

authors： Pfeiffer D,Schüler D

更新日期：2020-01-21 00:00:00

abstract：:We report the characterization of the ccpA gene of Lactobacillus plantarum, coding for catabolite control protein A. The gene is linked to the pepQ gene, encoding a proline peptidase, in the order ccpA-pepQ, with the two genes transcribed in tandem from the same strand as distinct transcriptional units. Two ccpA trans...

journal_title：Applied and environmental microbiology

authors： Muscariello L,Marasco R,De Felice M,Sacco M

更新日期：2001-07-01 00:00:00

abstract：:Different amounts of enterotoxin A-, B-, and C1-producing staphylococci were added to dry sausage prepared by normal processes, either alone or in conjunction with a starter culture (micrococci and lactobacilli). The sausage was examined after 0, 3, 7, 14, and 30 days for staphylococci, micrococci, and lactobacilli, a...

journal_title：Applied and environmental microbiology

authors： Niskanen A,Nurmi E

更新日期：1976-01-01 00:00:00

abstract：:The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficienc...

journal_title：Applied and environmental microbiology

authors： Eom GT,Song JK,Ahn JH,Seo YS,Rhee JS

更新日期：2005-07-01 00:00:00

abstract：:The biological impact of 24-h ("chronic") chromium(VI) [Cr(VI) or chromate] exposure on Shewanella oneidensis MR-1 was assessed by analyzing cellular morphology as well as genome-wide differential gene and protein expression profiles. Cells challenged aerobically with an initial chromate concentration of 0.3 mM in com...

journal_title：Applied and environmental microbiology

authors： Chourey K,Thompson MR,Morrell-Falvey J,Verberkmoes NC,Brown SD,Shah M,Zhou J,Doktycz M,Hettich RL,Thompson DK

更新日期：2006-09-01 00:00:00

abstract：:Mineral soil-based inoculants of Rhizobium meliloti and Rhizobium phaseoli survived better at 4 degrees C than at higher temperatures, but ca. 15% of the cells were viable at 37 degrees C after 27 days. Soil-based inoculants of R. meliloti, R. phaseoli, Rhizobium japonicum, and a cowpea Rhizobium sp. applied to seeds ...

journal_title：Applied and environmental microbiology

authors： Chao WL,Alexander M

更新日期：1984-01-01 00:00:00

abstract：:The effects of various nitrogen sources and of the cultivation time on the biosynthesis of N-labelled azoverdin were studied in batch cultures of Azomonas macrocytogenes ATCC 12334. Growth for 48 h in the presence of ammonium sulfate (0.2 g/liter) resulted in 95% labelling of azoverdin with N. A growth-related synthes...

journal_title：Applied and environmental microbiology

authors： Dobisová M,Abdallah MA,Kyslík P

更新日期：1994-12-01 00:00:00

abstract：:We investigated previously unknown associations between bacterial endosymbionts and bat flies of the subfamily Nycterophiliinae (Diptera, Streblidae). Molecular analyses revealed a novel clade of Gammaproteobacteria in Nycterophilia bat flies. This clade was not closely related to Arsenophonus-like microbes found in i...

journal_title：Applied and environmental microbiology

authors： Morse SF,Dick CW,Patterson BD,Dittmar K

更新日期：2012-12-01 00:00:00

abstract：:A negative enrichment procedure was developed which was capable of isolating Legionella pneumophila directly from seeded air-conditioning cooling tower water onto laboratory media. This procedure was based on an 8-h incubation under conditions that were bactericidal to the indigenous water microflora but merely bacter...

journal_title：Applied and environmental microbiology

authors： Thorpe TC,Miller RD

更新日期：1980-10-01 00:00:00

abstract：:Total extracted DNA from two heavily polychlorobiphenyl-contaminated soils was analyzed with respect to biphenyl dioxygenase sequences and activities. This was done by PCR amplification and cloning of a DNA segment encoding the active site of the enzyme. The translated sequences obtained fell into three similarity clu...

journal_title：Applied and environmental microbiology

authors： Standfuss-Gabisch C,Al-Halbouni D,Hofer B

更新日期：2012-04-01 00:00:00

abstract：:β-Aminopeptidases have the unique capability to hydrolyze N-terminal β-amino acids, with varied preferences for the nature of β-amino acid side chains. This unique capability makes them useful as biocatalysts for synthesis of β-peptides and to kinetically resolve β-peptides and amides for the production of enantiopure...

journal_title：Applied and environmental microbiology

authors： John-White M,Gardiner J,Johanesen P,Lyras D,Dumsday G

更新日期：2019-07-18 00:00:00

abstract：:Toxin-producing isolates of Bacillus licheniformis were obtained from foods involved in food poisoning incidents, from raw milk, and from industrially produced baby food. The toxin detection method, based on the inhibition of boar spermatozoan motility, has been shown previously to be a sensitive assay for the emetic ...

journal_title：Applied and environmental microbiology

authors： Salkinoja-Salonen MS,Vuorio R,Andersson MA,Kämpfer P,Andersson MC,Honkanen-Buzalski T,Scoging AC

更新日期：1999-10-01 00:00:00