Effect of starter culture on staphylococcal enterotoxin and thermonuclease production in dry sausage.

abstract：:The promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene (gpd) was used to drive expression of mnp1, the gene encoding Mn peroxidase isozyme 1, in primary metabolic cultures of Phanerochaete chrysosporium. A 1,100-bp fragment of the P. chrysosporium gpd promoter region was fused upstream of the mnp1 ge...

journal_title：Applied and environmental microbiology

authors： Mayfield MB,Kishi K,Alic M,Gold MH

更新日期：1994-12-01 00:00:00

abstract：:The presence of PCR inhibitors in water samples is well known and contributes to the fact that a practical PCR assay has not been developed for legionella surveillance. In this study, we devised a new seminested PCR assay for detection of Legionella spp. in water samples as a means of overriding the PCR inhibitors wit...

journal_title：Applied and environmental microbiology

authors： Miyamoto H,Yamamoto H,Arima K,Fujii J,Maruta K,Izu K,Shiomori T,Yoshida S

更新日期：1997-07-01 00:00:00

abstract：:Blooms of the cyanobacterium Anabaena circinalis are a major worldwide problem due to their production of a range of toxins, in particular the neurotoxins anatoxin-a and paralytic shellfish poisons (PSPs). Although there is a worldwide distribution of A. circinalis, there is a geographical segregation of neurotoxin pr...

journal_title：Applied and environmental microbiology

authors： Beltran EC,Neilan BA

更新日期：2000-10-01 00:00:00

abstract：:Three species of colonic bacteria can ferment the mucopolysaccharide chondroitin sulfate: Bacteroides ovatus, Bacteroides sp. strain 3452A (an unnamed DNA homology group), and B. thetaiotaomicron. Proteins associated with the utilization of chondroitin sulfate by B. thetaiotaomicron have been characterized previously....

journal_title：Applied and environmental microbiology

authors： Lipeski L,Guthrie EP,O'Brien M,Kotarski SF,Salyers AA

更新日期：1986-05-01 00:00:00

abstract：:Biotransformation of 2-chlorophenol by a methanogenic sediment community resulted in the transient accumulation of phenol and benzoate. 3-Chlorobenzoate was a more persistent product of 2-chlorophenol metabolism. The anaerobic biotransformation of phenol to benzoate presumably occurred via para-carboxylation and dehyd...

journal_title：Applied and environmental microbiology

authors： Becker JG,Stahl DA,Rittmann BE

更新日期：1999-11-01 00:00:00

abstract：:A methylomonad culture was isolated from pond water and examined as a potential source of single-cell protein. A medium containing magnesium sulfate, ammonium hydroxide, sodium phosphate, tap water, and methanol supported the growth of the isolate. Optimal growth conditions in batch cultures for the organism were: tem...

journal_title：Applied and environmental microbiology

authors： Chen BJ,Hirt W,Lim HC,Tsao GT

更新日期：1977-02-01 00:00:00

abstract：:A dot blot hybridization procedure with 32P-labeled whole chromosomal DNA of the type strains as probes was developed as a rapid and simple method for identification of intestinal Bacteroides species. Bacterial cells were fixed onto membrane filters by slight suction, treated with 0.5 N NaOH, and hybridized with these...

journal_title：Applied and environmental microbiology

authors： Morotomi M,Ohno T,Mutai M

更新日期：1988-05-01 00:00:00

abstract：:Genomic probes were used to investigate hepatitis A virus (HAV) and enterovirus RNAs in two types of shellfish from natural beds (Atlantic coast, France). After elution concentration, nucleic acid extracted by proteinase K and purified by phenol-chloroform and ethanol precipitation was assayed by dot blot hybridizatio...

journal_title：Applied and environmental microbiology

authors： Le Guyader F,Apaire-Marchais V,Brillet J,Billaudel S

更新日期：1993-11-01 00:00:00

abstract：:Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation o...

journal_title：Applied and environmental microbiology

authors： Billi D,Wright DJ,Helm RF,Prickett T,Potts M,Crowe JH

更新日期：2000-04-01 00:00:00

abstract：:Bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. Using the full-cycle rRNA approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bott...

journal_title：Applied and environmental microbiology

authors： Loy A,Beisker W,Meier H

更新日期：2005-07-01 00:00:00

abstract：:Nineteen Tn5-induced mutants of Rhizobium fredii HH303 defective in acidic exopolysaccharide synthesis were isolated by screening for lack of Calcofluor fluorescence. They were grouped by complementation analysis by using Rhizobium meliloti cosmids carrying exo genes. All of the 19 mutants were symbiotically effective...

journal_title：Applied and environmental microbiology

authors： Kim CH,Tully RE,Keister DL

更新日期：1989-07-01 00:00:00

abstract：:When microbes lack the nutrients necessary for growth, they enter stationary phase. In cases when energy sources are still present in the environment, they must decide whether to continue to use their metabolic program to harvest the available energy. Here we characterized the metabolic response to a variety of types ...

journal_title：Applied and environmental microbiology

authors： Chubukov V,Sauer U

更新日期：2014-05-01 00:00:00

abstract：:Different nutrient receptors varied in triggering germination of Bacillus subtilis spores with a pressure of 150 MPa, the GerA receptor being more responsive than the GerB receptor and even more responsive than the GerK receptor. This hierarchy in receptor responsiveness to pressure was the same as receptor responsive...

journal_title：Applied and environmental microbiology

authors： Black EP,Koziol-Dube K,Guan D,Wei J,Setlow B,Cortezzo DE,Hoover DG,Setlow P

更新日期：2005-10-01 00:00:00

abstract：:Clostridium difficile causes a potentially fatal diarrheal disease through the production of its principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a negative regulator of toxin production. Therefore, increased toxin production, and hence increased virulence, is often inferred in stra...

journal_title：Applied and environmental microbiology

authors： Cartman ST,Kelly ML,Heeg D,Heap JT,Minton NP

更新日期：2012-07-01 00:00:00

abstract：:The release of transgenic plants and microorganisms expressing truncated genes from various subspecies of Bacillus thuringiensis that encode active insecticidal toxins rather than inactive protoxins could result in the accumulation of these active proteins in soil, especially when bound on clays and other soil particl...

journal_title：Applied and environmental microbiology

authors： Tapp H,Stotzky G

更新日期：1995-05-01 00:00:00

abstract：:Despite a wealth of studies examining the toxicity of engineered nanomaterials, current knowledge on their cytotoxic mechanisms (particularly from a physical perspective) remains limited. In this work, we imaged and quantitatively characterized the biomechanical (hardness and elasticity), adhesive, and surface electri...

journal_title：Applied and environmental microbiology

authors： Zhang W,Hughes J,Chen Y

更新日期：2012-06-01 00:00:00

abstract：:Legionella pneumophila proliferates in freshwater environments at temperatures ranging from 25 to 45°C. To investigate the preference of different sequence types (ST) for a specific temperature range, growth of L. pneumophila serogroup 1 (SG1) ST1 (environmental strains), ST47, and ST62 (disease-associated strains) wa...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Brouwer-Hanzens AJ,Veenendaal HR,Wullings BA

更新日期：2016-10-27 00:00:00

abstract：:We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex geno...

journal_title：Applied and environmental microbiology

authors： Wang S,He J,Cui Z,Li S

更新日期：2007-08-01 00:00:00

abstract：:Acid-adapted strains of Escherichia coli K-12 W3110 were obtained by serial culture in medium buffered at pH 4.6 (M. M. Harden, A. He, K. Creamer, M. W. Clark, I. Hamdallah, K. A. Martinez, R. L. Kresslein, S. P. Bush, and J. L. Slonczewski, Appl Environ Microbiol 81:1932-1941, 2015, https://doi.org/10.1128/AEM.03494-...

journal_title：Applied and environmental microbiology

authors： He A,Penix SR,Basting PJ,Griffith JM,Creamer KE,Camperchioli D,Clark MW,Gonzales AS,Chávez Erazo JS,George NS,Bhagwat AA,Slonczewski JL

更新日期：2017-05-31 00:00:00

abstract：:Whole cells of the parent strain of Beijerinckia, grown with succinate and biphenyl, oxidized dibenzo-p-dioxin and several chlorinated dioxins. The rate of oxidation of the chlorinated dibenzo-p-dioxins decreased with an increasing degree of chlorine substitution. A mutant strain (B8/36) of Beijerinckia oxidized diben...

journal_title：Applied and environmental microbiology

authors： Klecka GM,Gibson DT

更新日期：1980-02-01 00:00:00

abstract：:Here we describe novel forms of structural integration between endo- and episymbiotic microbes and an unusual new species of snail from hydrothermal vents in the Indian Ocean. The snail houses a dense population of gamma-proteobacteria within the cells of its greatly enlarged esophageal gland. This tissue setting diff...

journal_title：Applied and environmental microbiology

authors： Goffredi SK,Warén A,Orphan VJ,Van Dover CL,Vrijenhoek RC

更新日期：2004-05-01 00:00:00

abstract：:We evaluated the restriction fragment length polymorphism of genomic DNA among 53 strains of the phytopathogenic bacterium Pseudomonas syringae pv. syringae. Twenty-nine strains were isolated from beans, and the rest were isolated from 11 other hosts. Southern blots of DNA digested with EcoRI or HindIII were hybridize...

journal_title：Applied and environmental microbiology

authors： Legard DE,Aquadro CF,Hunter JE

更新日期：1993-12-01 00:00:00

abstract：:Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was also at the apex of a...

journal_title：Applied and environmental microbiology

authors： Stamps BW,Nunn HS,Petryshyn VA,Oremland RS,Miller LG,Rosen MR,Bauer KW,Thompson KJ,Tookmanian EM,Waldeck AR,Loyd SJ,Johnson HA,Stevenson BS,Berelson WM,Corsetti FA,Spear JR

更新日期：2018-10-17 00:00:00

abstract：:We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the ass...

journal_title：Applied and environmental microbiology

authors： Sharma NK,Rees CE,Dodd CE

更新日期：2000-04-01 00:00:00

abstract：:Twenty known penicillic acid (PA)-producing Aspergillus and Penicillium cultures were grown under various conditions in shaken flasks to determine the highest yielding strains and their requirements for maximum toxin production. Abilities of the cultures to utilize eight different carbon sources in Raulin-Thom medium ...

journal_title：Applied and environmental microbiology

authors： Lindenfelser LA,Ciegler A

更新日期：1977-11-01 00:00:00

abstract：:Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner and outer membranes. In the present study, we developed a method by which the porin AlgE was detect...

journal_title：Applied and environmental microbiology

authors： Maleki S,Almaas E,Zotchev S,Valla S,Ertesvåg H

更新日期：2015-12-11 00:00:00

abstract：:The present study aimed at the long-term storage of rumen protozoa as living cells in liquid nitrogen. The two-step or interrupted slow freezing procedure was used to cryopreserve six of the dominant species of rumen ciliates isolated from monofaunated animals, Dasytricha ruminantium, Entodinium caudatum, Epidinium ec...

journal_title：Applied and environmental microbiology

authors： Nsabimana E,Kisidayová S,Macheboeuf D,Newbold CJ,Jouany JP

更新日期：2003-07-01 00:00:00

abstract：:Both Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis produce mosquitocidal toxins during sporulation and are extensively used in the field for control of mosquito populations. All the known toxins of the latter organism are known to be encoded on a large plasmid, pBtoxis. In an attempt to combine the...

journal_title：Applied and environmental microbiology

authors： Gammon K,Jones GW,Hope SJ,de Oliveira CM,Regis L,Silva Filha MH,Dancer BN,Berry C

更新日期：2006-03-01 00:00:00

abstract：:The cholera toxin genes of Vibrio cholerae are encoded by CTXΦ, a lysogenic bacteriophage. Infection with this phage plays a determinant role in toxigenicity conversion and the emergence of new clones of pathogenic V. cholerae. Multiple phage alleles, defined by sequence types of the repressor gene rstR, have been fou...

journal_title：Applied and environmental microbiology

authors： Wang H,Pang B,Xiong L,Wang D,Wang X,Zhang L,Kan B

更新日期：2015-10-01 00:00:00

abstract：:Nitrobacter agilis was entrapped in calcium alginate beads and used as a floating bed supplied with a continuous flow of nitrite medium. Complete nitrite oxidation was achieved within 30 h, and the system could be maintained for at least 210 h. The immobilized Nitrobacter system was subjected to sulfur oxyanions, acid...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Schlasner SM,Tuovinen OH

更新日期：1986-12-01 00:00:00